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The map label for this gene is tag [H]

Identifier: 167621949

GI number: 167621949

Start: 10636

End: 11196

Strand: Direct

Name: tag [H]

Synonym: Shal_0008

Alternate gene names: 167621949

Gene position: 10636-11196 (Clockwise)

Preceding gene: 167621946

Following gene: 167621958

Centisome position: 0.2

GC content: 41.89

Gene sequence:

>561_bases
ATGGAAGTAACGCGTTGCGACTGGGTTGGGGCGGATCCCATCTATCAAGAGTACCATGATAAGCTATGGGGAAAGCCTGT
GTTTGATAGTCTAGAGCTGTTTGAAAAGCTTTGTTTAGACGGTCAACAAGCGGGATTATCTTGGATTACCATTCTCAAGA
AACAGCAGAACTATGAAGCCGCATTTGCTAATTTTGACCCTAAAATTATTGTGACTTTTGACGATGAAAAGATAGAAGAG
TTGATGCTCAATCCAGGAATCGTACGTAATCGATTAAAGATAAACTCGATCATCAAAAACGCTAGGGCGTACCTTGAGTT
TATTGAGCAGGGTAATGATTTTTCAGCATTTTTATGGGAGTTTGTCGGCGGGGAACCTAAGCTTAATCACTTCACTTCTA
TGAGTGAGTTACCCGCGCAAACTCCAGAGTCAGAGGCTATGTCTAAGGCGTTAAAAAAACTAGGGTTCAACTTTGTCGGC
CCAACGATTTGCTACGCTTTTATGCAGGCCGTGGGTATGGTGAATGATCACACCACAGACTGTTTTTGTTATCAAAAATA
A

Upstream 100 bases:

>100_bases
TGGAAGCCTTAATAGAAAATGGTTTCGATTATACCTTGTAGAATAAGGCTTATGTAGGTTATTTTTTATCTGGTAGGTTA
AAAATTTAGGAATTAGGGCT

Downstream 100 bases:

>100_bases
CACGCATATTCTAGGAGTTTAAGGTTGGGTCATTACAAGTTGTGATGGATCTTCTGAATGCAAATAGATAGCTTTGAACT
CAACTTCACCCGCTGCATTA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MEVTRCDWVGADPIYQEYHDKLWGKPVFDSLELFEKLCLDGQQAGLSWITILKKQQNYEAAFANFDPKIIVTFDDEKIEE
LMLNPGIVRNRLKINSIIKNARAYLEFIEQGNDFSAFLWEFVGGEPKLNHFTSMSELPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHTTDCFCYQK

Sequences:

>Translated_186_residues
MEVTRCDWVGADPIYQEYHDKLWGKPVFDSLELFEKLCLDGQQAGLSWITILKKQQNYEAAFANFDPKIIVTFDDEKIEE
LMLNPGIVRNRLKINSIIKNARAYLEFIEQGNDFSAFLWEFVGGEPKLNHFTSMSELPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHTTDCFCYQK
>Mature_186_residues
MEVTRCDWVGADPIYQEYHDKLWGKPVFDSLELFEKLCLDGQQAGLSWITILKKQQNYEAAFANFDPKIIVTFDDEKIEE
LMLNPGIVRNRLKINSIIKNARAYLEFIEQGNDFSAFLWEFVGGEPKLNHFTSMSELPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHTTDCFCYQK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=54.4444444444444, Blast_Score=219, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21328; Mature: 21328

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTRCDWVGADPIYQEYHDKLWGKPVFDSLELFEKLCLDGQQAGLSWITILKKQQNYEA
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
AFANFDPKIIVTFDDEKIEELMLNPGIVRNRLKINSIIKNARAYLEFIEQGNDFSAFLWE
HHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FVGGEPKLNHFTSMSELPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHTTD
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
CFCYQK
EEEECC
>Mature Secondary Structure
MEVTRCDWVGADPIYQEYHDKLWGKPVFDSLELFEKLCLDGQQAGLSWITILKKQQNYEA
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
AFANFDPKIIVTFDDEKIEELMLNPGIVRNRLKINSIIKNARAYLEFIEQGNDFSAFLWE
HHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FVGGEPKLNHFTSMSELPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHTTD
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
CFCYQK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]