| Definition | Shewanella halifaxensis HAW-EB4 chromosome, complete genome. |
|---|---|
| Accession | NC_010334 |
| Length | 5,226,917 |
Click here to switch to the map view.
The map label for this gene is gyrB [H]
Identifier: 167621945
GI number: 167621945
Start: 3881
End: 6322
Strand: Direct
Name: gyrB [H]
Synonym: Shal_0004
Alternate gene names: 167621945
Gene position: 3881-6322 (Clockwise)
Preceding gene: 167621944
Following gene: 167621946
Centisome position: 0.07
GC content: 44.35
Gene sequence:
>2442_bases TTGATAAACCGACGAGAGAATAATATGTCAGAGAATAGTTACGATTCTTCGAGCATCAAGGTTCTAAAGGGCCTTGATGC GGTACGTAAGAGACCAGGAATGTACATCGGTGACACCGATGATGGTACAGGTCTACATCACATGGTATTCGAAGTGGTCG ATAACTCTATCGATGAAGCGTTAGCGGGCCACTGTAACGACGTCATAATTACCATTCATGCCGATGGTTCTGTATCGGTT CGAGATGACGGCCGTGGTATTCCGGTATCGATCCATGAAGAGGAAGGTATCTCTGCTGCTGAAGTTATTATGACGGTACT TCACGCTGGTGGTAAATTCGATGATAACTCTTATAAAGTATCGGGTGGTTTGCACGGTGTGGGCGTGTCAGTTGTAAACG CACTATCTTCAAAATTGCAGATGACTATTCGTCGTGACGGCAAGGTATATGAGCAGTTTTATACTCACGGTGTACCTGAT GCTCCGATTGCACCTATTGGTGAAGCGACTAAGACTGGTACTGAAATCCGTTTTTGGCCAAGTACAGAAACCTTTACTGA TGTCTTATTCCATTTTGATATTCTAGCTAAGCGTGTTCGTGAACTGTCATTTTTGAACTCAGGTGTTGGTATTCGTCTTG TTGACGAGCGTGATAACCGTGATGAGTTTTTCAAATACGAAGGCGGTATCAGTGCATTTGTTGACTACTTAAACGTTAAC AAAACACCGGTAAACAAAGAAGTATTCCACTTCTGCCAAGAGCGTGAAGACGGTATTACCGTTGAAGTCGCTATGCAGTG GAACGACGGTTTCCAAGAAAACATTTTCTGTTTTACCAACAACATTCCACAGCGCGATGGTGGTACTCACTTAGCGGGCT TCCGTGGTGCGTTGACACGTAACCTAAATAACTACATGGAACGTGAAGGGTATAACAAGAAAGGTAAGACTGCTGCAACG GGTGACGATGCTCGTGAAGGTTTAACGGCTGTTATCTCTGTTAAAGTACCTGATCCTAAGTTCAGCTCTCAGACTAAAGA CAAGCTTGTTTCAAGTGAAGTTAAGTCGGCAGTAGAACAAACAATGGGTGAGCAGTTAGGTGATTACCTAATGGAGCATC CAAATGAAGCTAAGATGATCGTTGGTAAGATTATCGACGCTGCGCGTGCTCGTGAAGCGGCGCGTAAAGCCCGTGAAATG ACTCGTCGTAAAGGCGCATTAGATTTAGGTGGTTTGCCAGGTAAACTTGCTGACTGCCAAGAGAAGGACCCTGGTCTTTC TGAAATTTACATAGTGGAGGGTGATTCTGCGGGCGGTAGTGCTAAGCAGGGTCGTAACCGTAAGAACCAAGCGATTCTGC CACTTAAAGGTAAGATTCTAAACGTAGAAAAAGCCCGTTTTGACAAGATGCTATCTTCTCAAGAAGTGGCAACACTGATC ACCGCACTAGGTTGTGGTATTGGTCGTGATGAATATAACCCAGATAAGACGCGTTACCATAACATCGTCATCATGACCGA TGCTGACGTCGACGGCTCGCACATTCGTACACTGCTGTTGACCTTCTTCTTCCGTCAAATGCCAGAGCTGATTGAACGTG GTTATGTTTATATTGCACAGCCACCTTTGTACAAAGTTAAAAAGGGTAAGCAAGAGCAGTATCTGAAAGATGATCCTGCA TTGACTGAGTACCTAACGACTCAAGCACTAGATAATGCCAATATCTATCCGTCTCAAGGTGCACCAGGCATGTCTGGTCA GCCACTAGAGCGTTTAGTTAATCAATATCGTGAAGTTGAATCTGTCATTGCTCGTCTAGAGCAGCGTTACCCAACTAACA TCACTGATCTAATGCTATATCACCCTGCAATCACTAATGAGATGTTGAGTGATGAAGCGCAAATTAAAGCTTGGACTGAT GTGTTTGTCGCTGACTTAATTGACAAAGAGTCTGGTGGTATCTTATATAGTGCTGAGCCAGTACTCGATCCTGAGCGTCA GGTTTACTTACCAAAGATTACTATCCGTAAGCATGGTATCGATACACACTACATGTTCTCGTATGACTTCTTCCAGTCTA CCGATTACCAGCGCATCGTTAAGATGGGTGCAGAAATCAATGGTATGGTTGAAGCGAGTGGTTACGTGCAACGTGGTGAG CGTACTAAGGATGTAACAAGCTTTGTTGAAGCTCTTGATTGGTTGATGAACGAATCTAAACGTGGTTTATACATTCAGCG TTATAAGGGATTGGGTGAGATGAACCCTGAGCAGTTGTGGGAAACGACAATGGATCCTGAATCTCGTCGCATGTTGCAAG TGACTATTGAAGACGCGATTGGTGCAGATCAACTGTTTACCTGTTTGATGGGTGATCAAGTAGAACCTCGTCGTGATTTC ATTGAAGCCAATGCTCTGAATGTGGCGAACCTCGACGTTTAA
Upstream 100 bases:
>100_bases CAAGTTTTTGTCACCGCTATCGAGCCTGCAGCAATAGTCGATTCGTTAGCCACACCACCAAGTAAGATGTTCCATGTGGA ACAGGGGTGTGTAACGGTAA
Downstream 100 bases:
>100_bases GTTTATTATAGGTAAACTTAGAAATTGAGTGAAATCGGTGCTATATGCACCGATTTTTTTTAATAATTCTGGCGTGGTTA TAAGTAAATTGTTTAGTAAA
Product: DNA gyrase subunit B
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 813; Mature: 813
Protein sequence:
>813_residues MINRRENNMSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDVIITIHADGSVSV RDDGRGIPVSIHEEEGISAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQMTIRRDGKVYEQFYTHGVPD APIAPIGEATKTGTEIRFWPSTETFTDVLFHFDILAKRVRELSFLNSGVGIRLVDERDNRDEFFKYEGGISAFVDYLNVN KTPVNKEVFHFCQEREDGITVEVAMQWNDGFQENIFCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEREGYNKKGKTAAT GDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEQLGDYLMEHPNEAKMIVGKIIDAARAREAARKAREM TRRKGALDLGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLI TALGCGIGRDEYNPDKTRYHNIVIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKQEQYLKDDPA LTEYLTTQALDNANIYPSQGAPGMSGQPLERLVNQYREVESVIARLEQRYPTNITDLMLYHPAITNEMLSDEAQIKAWTD VFVADLIDKESGGILYSAEPVLDPERQVYLPKITIRKHGIDTHYMFSYDFFQSTDYQRIVKMGAEINGMVEASGYVQRGE RTKDVTSFVEALDWLMNESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIGADQLFTCLMGDQVEPRRDF IEANALNVANLDV
Sequences:
>Translated_813_residues MINRRENNMSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDVIITIHADGSVSV RDDGRGIPVSIHEEEGISAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQMTIRRDGKVYEQFYTHGVPD APIAPIGEATKTGTEIRFWPSTETFTDVLFHFDILAKRVRELSFLNSGVGIRLVDERDNRDEFFKYEGGISAFVDYLNVN KTPVNKEVFHFCQEREDGITVEVAMQWNDGFQENIFCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEREGYNKKGKTAAT GDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEQLGDYLMEHPNEAKMIVGKIIDAARAREAARKAREM TRRKGALDLGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLI TALGCGIGRDEYNPDKTRYHNIVIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKQEQYLKDDPA LTEYLTTQALDNANIYPSQGAPGMSGQPLERLVNQYREVESVIARLEQRYPTNITDLMLYHPAITNEMLSDEAQIKAWTD VFVADLIDKESGGILYSAEPVLDPERQVYLPKITIRKHGIDTHYMFSYDFFQSTDYQRIVKMGAEINGMVEASGYVQRGE RTKDVTSFVEALDWLMNESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIGADQLFTCLMGDQVEPRRDF IEANALNVANLDV >Mature_813_residues MINRRENNMSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDVIITIHADGSVSV RDDGRGIPVSIHEEEGISAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQMTIRRDGKVYEQFYTHGVPD APIAPIGEATKTGTEIRFWPSTETFTDVLFHFDILAKRVRELSFLNSGVGIRLVDERDNRDEFFKYEGGISAFVDYLNVN KTPVNKEVFHFCQEREDGITVEVAMQWNDGFQENIFCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEREGYNKKGKTAAT GDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEQLGDYLMEHPNEAKMIVGKIIDAARAREAARKAREM TRRKGALDLGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLI TALGCGIGRDEYNPDKTRYHNIVIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKQEQYLKDDPA LTEYLTTQALDNANIYPSQGAPGMSGQPLERLVNQYREVESVIARLEQRYPTNITDLMLYHPAITNEMLSDEAQIKAWTD VFVADLIDKESGGILYSAEPVLDPERQVYLPKITIRKHGIDTHYMFSYDFFQSTDYQRIVKMGAEINGMVEASGYVQRGE RTKDVTSFVEALDWLMNESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIGADQLFTCLMGDQVEPRRDF IEANALNVANLDV
Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]
COG id: COG0187
COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI19913406, Length=572, Percent_Identity=23.7762237762238, Blast_Score=124, Evalue=3e-28, Organism=Homo sapiens, GI19913408, Length=562, Percent_Identity=25.2669039145907, Blast_Score=121, Evalue=3e-27, Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=71.1083437110834, Blast_Score=1192, Evalue=0.0, Organism=Escherichia coli, GI1789408, Length=557, Percent_Identity=40.2154398563734, Blast_Score=378, Evalue=1e-105, Organism=Caenorhabditis elegans, GI17535065, Length=549, Percent_Identity=26.4116575591985, Blast_Score=132, Evalue=9e-31, Organism=Caenorhabditis elegans, GI212645845, Length=510, Percent_Identity=25.6862745098039, Blast_Score=115, Evalue=1e-25, Organism=Caenorhabditis elegans, GI212645657, Length=165, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6324241, Length=575, Percent_Identity=25.3913043478261, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI17136538, Length=546, Percent_Identity=26.1904761904762, Blast_Score=136, Evalue=5e-32,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003594 - InterPro: IPR011557 - InterPro: IPR020568 - InterPro: IPR014721 - InterPro: IPR001241 - InterPro: IPR013759 - InterPro: IPR002288 - InterPro: IPR013506 - InterPro: IPR013760 - InterPro: IPR018522 - InterPro: IPR006171 [H]
Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]
EC number: =5.99.1.3 [H]
Molecular weight: Translated: 90975; Mature: 90975
Theoretical pI: Translated: 5.04; Mature: 5.04
Prosite motif: PS00177 TOPOISOMERASE_II
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MINRRENNMSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH LAGHCNDVIITIHADGSVSVRDDGRGIPVSIHEEEGISAAEVIMTVLHAGGKFDDNSYKV HHCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEE SGGLHGVGVSVVNALSSKLQMTIRRDGKVYEQFYTHGVPDAPIAPIGEATKTGTEIRFWP CCCCCHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC STETFTDVLFHFDILAKRVRELSFLNSGVGIRLVDERDNRDEFFKYEGGISAFVDYLNVN CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEECCCHHHHHHHHCCC KTPVNKEVFHFCQEREDGITVEVAMQWNDGFQENIFCFTNNIPQRDGGTHLAGFRGALTR CCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH NLNNYMEREGYNKKGKTAATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQ HHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH TMGEQLGDYLMEHPNEAKMIVGKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQ HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC EKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLI CCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHH TALGCGIGRDEYNPDKTRYHNIVIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQ HHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEC PPLYKVKKGKQEQYLKDDPALTEYLTTQALDNANIYPSQGAPGMSGQPLERLVNQYREVE CCCHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH SVIARLEQRYPTNITDLMLYHPAITNEMLSDEAQIKAWTDVFVADLIDKESGGILYSAEP HHHHHHHHHCCCCHHHHHEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC VLDPERQVYLPKITIRKHGIDTHYMFSYDFFQSTDYQRIVKMGAEINGMVEASGYVQRGE CCCCCCEEEECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHCCCCCCEEECCCHHHCCC RTKDVTSFVEALDWLMNESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAI CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEHHHC GADQLFTCLMGDQVEPRRDFIEANALNVANLDV CHHHHHHHHHCCCCCHHHHHHHCCCCCEECCCC >Mature Secondary Structure MINRRENNMSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH LAGHCNDVIITIHADGSVSVRDDGRGIPVSIHEEEGISAAEVIMTVLHAGGKFDDNSYKV HHCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEE SGGLHGVGVSVVNALSSKLQMTIRRDGKVYEQFYTHGVPDAPIAPIGEATKTGTEIRFWP CCCCCHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC STETFTDVLFHFDILAKRVRELSFLNSGVGIRLVDERDNRDEFFKYEGGISAFVDYLNVN CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEECCCHHHHHHHHCCC KTPVNKEVFHFCQEREDGITVEVAMQWNDGFQENIFCFTNNIPQRDGGTHLAGFRGALTR CCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH NLNNYMEREGYNKKGKTAATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQ HHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH TMGEQLGDYLMEHPNEAKMIVGKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQ HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC EKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLI CCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHH TALGCGIGRDEYNPDKTRYHNIVIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQ HHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEC PPLYKVKKGKQEQYLKDDPALTEYLTTQALDNANIYPSQGAPGMSGQPLERLVNQYREVE CCCHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH SVIARLEQRYPTNITDLMLYHPAITNEMLSDEAQIKAWTDVFVADLIDKESGGILYSAEP HHHHHHHHHCCCCHHHHHEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC VLDPERQVYLPKITIRKHGIDTHYMFSYDFFQSTDYQRIVKMGAEINGMVEASGYVQRGE CCCCCCEEEECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHCCCCCCEEECCCHHHCCC RTKDVTSFVEALDWLMNESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAI CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEHHHC GADQLFTCLMGDQVEPRRDFIEANALNVANLDV CHHHHHHHHHCCCCCHHHHHHHCCCCCEECCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]