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The map label for this gene is purA

Identifier: 167040965

GI number: 167040965

Start: 2396099

End: 2397379

Strand: Reverse

Name: purA

Synonym: Teth514_2354

Alternate gene names: 167040965

Gene position: 2397379-2396099 (Counterclockwise)

Preceding gene: 167040966

Following gene: 167040964

Centisome position: 97.56

GC content: 36.46

Gene sequence:

>1281_bases
ATGTCAACATTAGTTATAGTTGGTACCCAATGGGGAGATGAAGGGAAAGGGAAAATTACTGATTATCTTGCGGAAAAGGC
AGATGTAGTCGTAAGATACCAAGGAGGGAACAACGCAGGGCATACTGTTGAAAAAGAAGGTGTCCAGTATAAACTTCATT
TAATTCCTTCTGGAATATTGTACTCCGAAAAAATTTGCATAATAGGTAATGGGGTAGTGGTTGACCCTGCTTCTTTGATT
GAGGAAATAGAAAATTTACAAAAACAAGGAATTAGCGTGGATAATTTAAAGATAAGCGATAGAGCCCATATCGTTTTTCC
TTATCACATAAAGCAAGATGAACTTGAAGAGATTTCAAAAGGGAAAAACGACTTAGGGACAACAAAAAGAGGAATTGGTC
CTTGTTACATGGATAAGTCAGAAAGAATAGGGATAAGGGTTTGTGACCTTCTAAAACCCAAAGTTTTTGAAGAAAAACTT
AGAAGAAATGTGGAAAAGAAAAACAAACTTTTTAAGGAGCTATATGGAGCAGAAGGATTTGACTTTGAAGAAATGTACCA
AAAATACTTGGAGTATGCTGAAAAAATAAAACCCTTTGTTACTGATACAACAGTGCTTTTATACGATTTAATTAAAAGTG
GCAAAAAAGTTTTATTTGAAGGAGCACAGGGGACACTTCTTGATTTAGATTTGGGTACCTATCCTTATGTTACTGCTTCC
CATCCTATAGCAGGCGGTGTTACAGTGGGGGCTGGCATAGGACCCACGATGATTGATGAAGTTATGGGAGTTGTGAAGGC
GTATACTACGAGGGTTGGAAAAGGCCCATTTCCTACAGAGCTTTTTGATGAAAATGGTGAATTTTTAAGAGAAAAAGGCC
ATGAATACGGTACTACAACGGGAAGAGCCAGAAGATGTGGCTGGCTAGATGCTGTGATTCTTAAATACTCTGTAAGAGTA
TCCGGCATAACACATTTTGCTTTGACAAAACTTGATACCCTGACAGGGCTTAAAAAAATAAAAATTTGTACAGGATATAA
ATTTAATGGCAGAATAATAACAGATTTTCCCGCAAGTCTTGAGGATTTGGCTCAATGTGAACCTGTATATGAAGAATTTG
ACGGGTGGGAAGAAGATATACAAGGAGCAAAAACTTTTGACGACTTACCTTATAATGCTCAAAAATATATAAGAAGAATT
GAAGAATTGATAGGCATTAAAGCAGCAATAATATCTGTAGGACCAGAAAGAAATCAGACTATTGTTTTAAGGGATTTTTG
A

Upstream 100 bases:

>100_bases
TGGAGCAGGCATTACACGGGGACTGGCACAAGCCTCTGTATCTGGCGTTGTAGTTGCAAGAGAAATATTGGAAAGAGTTA
AATAAGGAAGGAGAATAATT

Downstream 100 bases:

>100_bases
TGAAGTTTCATCAAAATCCCTTTTTGTTTGTTGACTGTAACAACCTTAAATGGTATATTTTAAATATAAAGGTTTAACTA
AAAGGGGGATTGCAAATTGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSTLVIVGTQWGDEGKGKITDYLAEKADVVVRYQGGNNAGHTVEKEGVQYKLHLIPSGILYSEKICIIGNGVVVDPASLI
EEIENLQKQGISVDNLKISDRAHIVFPYHIKQDELEEISKGKNDLGTTKRGIGPCYMDKSERIGIRVCDLLKPKVFEEKL
RRNVEKKNKLFKELYGAEGFDFEEMYQKYLEYAEKIKPFVTDTTVLLYDLIKSGKKVLFEGAQGTLLDLDLGTYPYVTAS
HPIAGGVTVGAGIGPTMIDEVMGVVKAYTTRVGKGPFPTELFDENGEFLREKGHEYGTTTGRARRCGWLDAVILKYSVRV
SGITHFALTKLDTLTGLKKIKICTGYKFNGRIITDFPASLEDLAQCEPVYEEFDGWEEDIQGAKTFDDLPYNAQKYIRRI
EELIGIKAAIISVGPERNQTIVLRDF

Sequences:

>Translated_426_residues
MSTLVIVGTQWGDEGKGKITDYLAEKADVVVRYQGGNNAGHTVEKEGVQYKLHLIPSGILYSEKICIIGNGVVVDPASLI
EEIENLQKQGISVDNLKISDRAHIVFPYHIKQDELEEISKGKNDLGTTKRGIGPCYMDKSERIGIRVCDLLKPKVFEEKL
RRNVEKKNKLFKELYGAEGFDFEEMYQKYLEYAEKIKPFVTDTTVLLYDLIKSGKKVLFEGAQGTLLDLDLGTYPYVTAS
HPIAGGVTVGAGIGPTMIDEVMGVVKAYTTRVGKGPFPTELFDENGEFLREKGHEYGTTTGRARRCGWLDAVILKYSVRV
SGITHFALTKLDTLTGLKKIKICTGYKFNGRIITDFPASLEDLAQCEPVYEEFDGWEEDIQGAKTFDDLPYNAQKYIRRI
EELIGIKAAIISVGPERNQTIVLRDF
>Mature_425_residues
STLVIVGTQWGDEGKGKITDYLAEKADVVVRYQGGNNAGHTVEKEGVQYKLHLIPSGILYSEKICIIGNGVVVDPASLIE
EIENLQKQGISVDNLKISDRAHIVFPYHIKQDELEEISKGKNDLGTTKRGIGPCYMDKSERIGIRVCDLLKPKVFEEKLR
RNVEKKNKLFKELYGAEGFDFEEMYQKYLEYAEKIKPFVTDTTVLLYDLIKSGKKVLFEGAQGTLLDLDLGTYPYVTASH
PIAGGVTVGAGIGPTMIDEVMGVVKAYTTRVGKGPFPTELFDENGEFLREKGHEYGTTTGRARRCGWLDAVILKYSVRVS
GITHFALTKLDTLTGLKKIKICTGYKFNGRIITDFPASLEDLAQCEPVYEEFDGWEEDIQGAKTFDDLPYNAQKYIRRIE
ELIGIKAAIISVGPERNQTIVLRDF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=46.8384074941452, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=47.7855477855478, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=45.7286432160804, Blast_Score=339, Evalue=3e-93,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=49.0610328638498, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=45.9016393442623, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=45.9016393442623, Blast_Score=356, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=44.2890442890443, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=44.8931116389549, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THEP3 (B0K8D7)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001666205.1
- ProteinModelPortal:   B0K8D7
- SMR:   B0K8D7
- GeneID:   5873740
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_2248
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   TPSE340099:TETH39_2248-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47632; Mature: 47501

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVIVGTQWGDEGKGKITDYLAEKADVVVRYQGGNNAGHTVEKEGVQYKLHLIPSGIL
CCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCEEEEEEECCCCE
YSEKICIIGNGVVVDPASLIEEIENLQKQGISVDNLKISDRAHIVFPYHIKQDELEEISK
EECEEEEEECCEEECHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GKNDLGTTKRGIGPCYMDKSERIGIRVCDLLKPKVFEEKLRRNVEKKNKLFKELYGAEGF
CCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DFEEMYQKYLEYAEKIKPFVTDTTVLLYDLIKSGKKVLFEGAQGTLLDLDLGTYPYVTAS
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCEEECC
HPIAGGVTVGAGIGPTMIDEVMGVVKAYTTRVGKGPFPTELFDENGEFLREKGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCCCCCCCC
GRARRCGWLDAVILKYSVRVSGITHFALTKLDTLTGLKKIKICTGYKFNGRIITDFPASL
CCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCCEEEEEECEEECCEEEECCCCCH
EDLAQCEPVYEEFDGWEEDIQGAKTFDDLPYNAQKYIRRIEELIGIKAAIISVGPERNQT
HHHHHCCHHHHHHCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCE
IVLRDF
EEEECC
>Mature Secondary Structure 
STLVIVGTQWGDEGKGKITDYLAEKADVVVRYQGGNNAGHTVEKEGVQYKLHLIPSGIL
CEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCEEEEEEECCCCE
YSEKICIIGNGVVVDPASLIEEIENLQKQGISVDNLKISDRAHIVFPYHIKQDELEEISK
EECEEEEEECCEEECHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GKNDLGTTKRGIGPCYMDKSERIGIRVCDLLKPKVFEEKLRRNVEKKNKLFKELYGAEGF
CCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DFEEMYQKYLEYAEKIKPFVTDTTVLLYDLIKSGKKVLFEGAQGTLLDLDLGTYPYVTAS
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCEEECC
HPIAGGVTVGAGIGPTMIDEVMGVVKAYTTRVGKGPFPTELFDENGEFLREKGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCCCCCCCC
GRARRCGWLDAVILKYSVRVSGITHFALTKLDTLTGLKKIKICTGYKFNGRIITDFPASL
CCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCCEEEEEECEEECCEEEECCCCCH
EDLAQCEPVYEEFDGWEEDIQGAKTFDDLPYNAQKYIRRIEELIGIKAAIISVGPERNQT
HHHHHCCHHHHHHCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCE
IVLRDF
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA