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The map label for this gene is mcrB [H]

Identifier: 167040884

GI number: 167040884

Start: 2267742

End: 2270153

Strand: Reverse

Name: mcrB [H]

Synonym: Teth514_2266

Alternate gene names: 167040884

Gene position: 2270153-2267742 (Counterclockwise)

Preceding gene: 167040885

Following gene: 167040883

Centisome position: 92.39

GC content: 30.27

Gene sequence:

>2412_bases
ATGAAGATTGAAGATTTAAAAGGAAAATTTTTTATGGACAATTATATGGACATTGCTATTGAGGAATATTTAAAGTTCAA
AGAAACTGTGGATTACGATGAAAAGTATAAAAGAGAAGTTTTAAGCGAAATAAATGATTATTTAAAAGACAAGCGTATTG
CTGGGGAGAATGTTATAGAAATAATTAAGTATTTTCAAAGTAAAAATCCACAAAAGGGTGGTTTTGTTCATTGGTCGAAT
TTAGATGATTTATATAAATATGCAGAAGAAAAACCTTCAGAAGTAGCTGAATTGTTTAACTATCTATATGATGATTCGGC
AGATTTAGAAGATAGAATACGAAGATTTGTGAATACTGGGGAAAGATATAATACTACTATTAAATTTGATACTTCTCTTT
TTGGATATTTATTAGCAGGTTTTAGTTTAGAGAAGTATCCCTTGTATAGGGATCAGGCATTTAGAGAGTTTTTAAATTCT
TTTGGAATTAATGAGTCTTTAACTGAAGATGTAGCAGATAAGTATTCTACCTATTATGAAGTTTGTAATATTTTGCTCGA
TTATTTTAAAGAGAAAAATTATATAAAAAATCCAGATATGTTGGATGCACAGGATTTCATTTATTGTCTTACCAACTACA
AGGAACTGTATGTAAAAGTAAGTGTAAAGTATCTCTATGAACATGCAAAGGTATTGTATTCTTTTAGTAAGGATATAAAT
GCTTTTTTGGAATATTTAAAGGAATTAGATAGAGAATATTTACAAGTTTTATATGAAAGATACAAAGGTGATGAAAAAGT
CAATGAAATTAGATTTAGAGTGTGTGAGATGATTTTAAATGGTGATTATGTGGATGAGAAAAATTTGGAGAGCATAAAGA
ATGAAGTTGCAAATAAACATGAAAAAGATATTTTAAAATCATGGAATGATTTTAAGATTTTGTTTCCATTATATTATGAA
AAGTATAAGGAAAAAGTGAATATAGAAATTAGAAAAATTTACAATAGTATTAAATCGATATTTCCTAATTTTAAATTTAA
AGAAAATAAATATATTAGTGATTTTTATGGACCACAGAATTTTGGAGGGAGTAGATGTTGGTTTGCAATATATCCCGAAA
AGAAAAAAACACATAAAGAAGCTGCTCAGTTATTTTTTACAATTGGGGGAGATGAAACGGAAAAGTATTCTAATATTTCG
TATGGTTTATATATAGGTGATGATATTAATAAAATTCTTGAAGAGAAAGGCACCAAAGAACACGATGATATAGAAACCAT
TGATAACCCTAATGATTTTACATATAAGAAAATGGTGAAGAAGTTTATTGAAGTTTTTGATAGGTTTAAAAAGATAAATG
GTATAAATAGAGAGGACATTATTAAGGTGTCTCATGGGGAAAAGGATAAAAGCGGCGAGGAGGAGAAAGATGGCTTTCTT
CATGAAATTGATTTTGAAAGAGAAATTAAAATAAATAATTTATTTTTTGAGGATGCAGAGGTTTTATCAAAGCAAATATC
TACTGCTTTAAAATCAGGTAAACACATAATATTGGTAGGTCCTCCTGGAACAGGCAAGTCCAAACTAGCTAAAGAAATAT
GTAAAAGTTACGGAGTAGAATACGAAATGGTGACTGCCATGTCCGATTGGTCTACTTATGATACTATTGGTGGTTATAAA
CCTGATAGGGATGGAACTTTGTATTTTGATGAAGGCGTATTTTTACGGCTTTTTAAAAATAAGAAAAATAACAATTCCAA
TATCAAGTGGCTAATTATAGATGAAATAAACAGAGCTGATATTGACAAAGCTTTTGGTGTATTATTTTCTGCCTTAACAG
GGGACAGGGTGACACTGAGTTTTAAGTCAAAATCTGGAGAGAATATAGTCATACGTCCGGAGGAAGATGATGAAGAAGAG
GTAGAGATACAAGAACATGGGTATGTAATTCCTAAAGATTTTCGCATTATTGGGACTATGAACACTTACGACAAAACTTC
TTTATATGAAATGAGCTATGCCTTTATGAGGCGATTTGCTTTTATTCCTGTTGGTGTGCCAAAAAACATTGATAAGGACC
TTGTTGCGAAATATCTTAATATATGGGGGATAGAGGATAAAAGCATCAATAATGTGGATTTAAAAGAGGGACTGGCGGAG
GTTTGGAATATTATTAACGAATACAGAAGAGTTGGACCTGCTATTATTGAAGACATTGCCAGATATGTTAGCGTTGAAGG
GGATTACACTTCTGCTGTTATACTTTATGTCCTGCCGCAGTTCGAAGGCGTTATGGAAAATGATATTAAAAAATTTGCAG
AGGAATTGTATAAAAGCAGTATAGGAGATTTTTCTAGACAAAAGGATAGATTGGATGGATTCATAAAAGACTTTTTTGGA
ATAAAAATGTGA

Upstream 100 bases:

>100_bases
GGTAGTGCCTTTATTTTTTATGGAAATATAAAGGATTTTCAATAAATTTATAGAATAGATAATATAACATACATTAATGT
AGTTTTAGGGGGATCTTAGG

Downstream 100 bases:

>100_bases
TTTTTAGGGGATTTGACTATGAAGACAAGTAAAGAAGTGCTTTTAGAATCTATCAAAGAGGATTTATTGGTATATTTAAA
GTCTGGCAAATTATCTCCGC

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MKIEDLKGKFFMDNYMDIAIEEYLKFKETVDYDEKYKREVLSEINDYLKDKRIAGENVIEIIKYFQSKNPQKGGFVHWSN
LDDLYKYAEEKPSEVAELFNYLYDDSADLEDRIRRFVNTGERYNTTIKFDTSLFGYLLAGFSLEKYPLYRDQAFREFLNS
FGINESLTEDVADKYSTYYEVCNILLDYFKEKNYIKNPDMLDAQDFIYCLTNYKELYVKVSVKYLYEHAKVLYSFSKDIN
AFLEYLKELDREYLQVLYERYKGDEKVNEIRFRVCEMILNGDYVDEKNLESIKNEVANKHEKDILKSWNDFKILFPLYYE
KYKEKVNIEIRKIYNSIKSIFPNFKFKENKYISDFYGPQNFGGSRCWFAIYPEKKKTHKEAAQLFFTIGGDETEKYSNIS
YGLYIGDDINKILEEKGTKEHDDIETIDNPNDFTYKKMVKKFIEVFDRFKKINGINREDIIKVSHGEKDKSGEEEKDGFL
HEIDFEREIKINNLFFEDAEVLSKQISTALKSGKHIILVGPPGTGKSKLAKEICKSYGVEYEMVTAMSDWSTYDTIGGYK
PDRDGTLYFDEGVFLRLFKNKKNNNSNIKWLIIDEINRADIDKAFGVLFSALTGDRVTLSFKSKSGENIVIRPEEDDEEE
VEIQEHGYVIPKDFRIIGTMNTYDKTSLYEMSYAFMRRFAFIPVGVPKNIDKDLVAKYLNIWGIEDKSINNVDLKEGLAE
VWNIINEYRRVGPAIIEDIARYVSVEGDYTSAVILYVLPQFEGVMENDIKKFAEELYKSSIGDFSRQKDRLDGFIKDFFG
IKM

Sequences:

>Translated_803_residues
MKIEDLKGKFFMDNYMDIAIEEYLKFKETVDYDEKYKREVLSEINDYLKDKRIAGENVIEIIKYFQSKNPQKGGFVHWSN
LDDLYKYAEEKPSEVAELFNYLYDDSADLEDRIRRFVNTGERYNTTIKFDTSLFGYLLAGFSLEKYPLYRDQAFREFLNS
FGINESLTEDVADKYSTYYEVCNILLDYFKEKNYIKNPDMLDAQDFIYCLTNYKELYVKVSVKYLYEHAKVLYSFSKDIN
AFLEYLKELDREYLQVLYERYKGDEKVNEIRFRVCEMILNGDYVDEKNLESIKNEVANKHEKDILKSWNDFKILFPLYYE
KYKEKVNIEIRKIYNSIKSIFPNFKFKENKYISDFYGPQNFGGSRCWFAIYPEKKKTHKEAAQLFFTIGGDETEKYSNIS
YGLYIGDDINKILEEKGTKEHDDIETIDNPNDFTYKKMVKKFIEVFDRFKKINGINREDIIKVSHGEKDKSGEEEKDGFL
HEIDFEREIKINNLFFEDAEVLSKQISTALKSGKHIILVGPPGTGKSKLAKEICKSYGVEYEMVTAMSDWSTYDTIGGYK
PDRDGTLYFDEGVFLRLFKNKKNNNSNIKWLIIDEINRADIDKAFGVLFSALTGDRVTLSFKSKSGENIVIRPEEDDEEE
VEIQEHGYVIPKDFRIIGTMNTYDKTSLYEMSYAFMRRFAFIPVGVPKNIDKDLVAKYLNIWGIEDKSINNVDLKEGLAE
VWNIINEYRRVGPAIIEDIARYVSVEGDYTSAVILYVLPQFEGVMENDIKKFAEELYKSSIGDFSRQKDRLDGFIKDFFG
IKM
>Mature_803_residues
MKIEDLKGKFFMDNYMDIAIEEYLKFKETVDYDEKYKREVLSEINDYLKDKRIAGENVIEIIKYFQSKNPQKGGFVHWSN
LDDLYKYAEEKPSEVAELFNYLYDDSADLEDRIRRFVNTGERYNTTIKFDTSLFGYLLAGFSLEKYPLYRDQAFREFLNS
FGINESLTEDVADKYSTYYEVCNILLDYFKEKNYIKNPDMLDAQDFIYCLTNYKELYVKVSVKYLYEHAKVLYSFSKDIN
AFLEYLKELDREYLQVLYERYKGDEKVNEIRFRVCEMILNGDYVDEKNLESIKNEVANKHEKDILKSWNDFKILFPLYYE
KYKEKVNIEIRKIYNSIKSIFPNFKFKENKYISDFYGPQNFGGSRCWFAIYPEKKKTHKEAAQLFFTIGGDETEKYSNIS
YGLYIGDDINKILEEKGTKEHDDIETIDNPNDFTYKKMVKKFIEVFDRFKKINGINREDIIKVSHGEKDKSGEEEKDGFL
HEIDFEREIKINNLFFEDAEVLSKQISTALKSGKHIILVGPPGTGKSKLAKEICKSYGVEYEMVTAMSDWSTYDTIGGYK
PDRDGTLYFDEGVFLRLFKNKKNNNSNIKWLIIDEINRADIDKAFGVLFSALTGDRVTLSFKSKSGENIVIRPEEDDEEE
VEIQEHGYVIPKDFRIIGTMNTYDKTSLYEMSYAFMRRFAFIPVGVPKNIDKDLVAKYLNIWGIEDKSINNVDLKEGLAE
VWNIINEYRRVGPAIIEDIARYVSVEGDYTSAVILYVLPQFEGVMENDIKKFAEELYKSSIGDFSRQKDRLDGFIKDFFG
IKM

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=202, Percent_Identity=33.1683168316832, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 94525; Mature: 94525

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEDLKGKFFMDNYMDIAIEEYLKFKETVDYDEKYKREVLSEINDYLKDKRIAGENVIE
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IIKYFQSKNPQKGGFVHWSNLDDLYKYAEEKPSEVAELFNYLYDDSADLEDRIRRFVNTG
HHHHHHCCCCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
ERYNTTIKFDTSLFGYLLAGFSLEKYPLYRDQAFREFLNSFGINESLTEDVADKYSTYYE
CCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VCNILLDYFKEKNYIKNPDMLDAQDFIYCLTNYKELYVKVSVKYLYEHAKVLYSFSKDIN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHEEHHHHHHHHHHHHHHHHHHHHH
AFLEYLKELDREYLQVLYERYKGDEKVNEIRFRVCEMILNGDYVDEKNLESIKNEVANKH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EKDILKSWNDFKILFPLYYEKYKEKVNIEIRKIYNSIKSIFPNFKFKENKYISDFYGPQN
HHHHHCCCCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
FGGSRCWFAIYPEKKKTHKEAAQLFFTIGGDETEKYSNISYGLYIGDDINKILEEKGTKE
CCCCEEEEEECCCHHHHHHHHHHEEEEECCCCCHHHCCCEEEEEECCHHHHHHHHCCCCC
HDDIETIDNPNDFTYKKMVKKFIEVFDRFKKINGINREDIIKVSHGEKDKSGEEEKDGFL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCHHCCCE
HEIDFEREIKINNLFFEDAEVLSKQISTALKSGKHIILVGPPGTGKSKLAKEICKSYGVE
EECCCCCEEEECCEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCC
YEMVTAMSDWSTYDTIGGYKPDRDGTLYFDEGVFLRLFKNKKNNNSNIKWLIIDEINRAD
EEEEEEHHCCCCHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCEEEEEEECCCHHH
IDKAFGVLFSALTGDRVTLSFKSKSGENIVIRPEEDDEEEVEIQEHGYVIPKDFRIIGTM
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCEECCCCCEEEECC
NTYDKTSLYEMSYAFMRRFAFIPVGVPKNIDKDLVAKYLNIWGIEDKSINNVDLKEGLAE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VWNIINEYRRVGPAIIEDIARYVSVEGDYTSAVILYVLPQFEGVMENDIKKFAEELYKSS
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHEEHEEEHHHHHHHHHHHHHHHHHHHHHHH
IGDFSRQKDRLDGFIKDFFGIKM
HCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIEDLKGKFFMDNYMDIAIEEYLKFKETVDYDEKYKREVLSEINDYLKDKRIAGENVIE
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IIKYFQSKNPQKGGFVHWSNLDDLYKYAEEKPSEVAELFNYLYDDSADLEDRIRRFVNTG
HHHHHHCCCCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
ERYNTTIKFDTSLFGYLLAGFSLEKYPLYRDQAFREFLNSFGINESLTEDVADKYSTYYE
CCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VCNILLDYFKEKNYIKNPDMLDAQDFIYCLTNYKELYVKVSVKYLYEHAKVLYSFSKDIN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHEEHHHHHHHHHHHHHHHHHHHHH
AFLEYLKELDREYLQVLYERYKGDEKVNEIRFRVCEMILNGDYVDEKNLESIKNEVANKH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EKDILKSWNDFKILFPLYYEKYKEKVNIEIRKIYNSIKSIFPNFKFKENKYISDFYGPQN
HHHHHCCCCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
FGGSRCWFAIYPEKKKTHKEAAQLFFTIGGDETEKYSNISYGLYIGDDINKILEEKGTKE
CCCCEEEEEECCCHHHHHHHHHHEEEEECCCCCHHHCCCEEEEEECCHHHHHHHHCCCCC
HDDIETIDNPNDFTYKKMVKKFIEVFDRFKKINGINREDIIKVSHGEKDKSGEEEKDGFL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCHHCCCE
HEIDFEREIKINNLFFEDAEVLSKQISTALKSGKHIILVGPPGTGKSKLAKEICKSYGVE
EECCCCCEEEECCEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCC
YEMVTAMSDWSTYDTIGGYKPDRDGTLYFDEGVFLRLFKNKKNNNSNIKWLIIDEINRAD
EEEEEEHHCCCCHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCEEEEEEECCCHHH
IDKAFGVLFSALTGDRVTLSFKSKSGENIVIRPEEDDEEEVEIQEHGYVIPKDFRIIGTM
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCEECCCCCEEEECC
NTYDKTSLYEMSYAFMRRFAFIPVGVPKNIDKDLVAKYLNIWGIEDKSINNVDLKEGLAE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VWNIINEYRRVGPAIIEDIARYVSVEGDYTSAVILYVLPQFEGVMENDIKKFAEELYKSS
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHEEHEEEHHHHHHHHHHHHHHHHHHHHHHH
IGDFSRQKDRLDGFIKDFFGIKM
HCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]