Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 167040775

GI number: 167040775

Start: 2155188

End: 2156771

Strand: Reverse

Name: appA [H]

Synonym: Teth514_2152

Alternate gene names: 167040775

Gene position: 2156771-2155188 (Counterclockwise)

Preceding gene: 167040776

Following gene: 167040774

Centisome position: 87.77

GC content: 29.29

Gene sequence:

>1584_bases
ATGAGAAAATTTATTATATTATCTTTCATTTTGATGTTTATTTTTACAGGATGCAAAACCACTCCACAAAATATGGTAAA
TTTGCCGCAAGCTTCTGAGGATAAAACTAAGATTGAAGAAGAAAAGCCACTAGAGGGTGGCACTTTAAGAGTAAATATTA
CTTCTTTTGATACGTTAAATCCTTTTTTAAACAGCAATGAACGAGTAAGGCAAATGTTGAATTTGTCATTGGAAGGATTA
GTTACTTTAGATAAAACTTTAAAACCTGTTCCTCAATTAGCAGAAAAGTGGGAGATAAATGGACTTAATATAAAATTTTA
TTTAAAAAAGAATGTTAAATGGCAGGATGGAGTAAGTTTTACAGCTAATGATGTAAAATTTACTTTTGATTCTTTTAAAA
GTAAGGAGGTAAAAAGCCCTTATAAGGACATTCTCATTAACTATCTCTCCTCTTATAAGACAAACGGTGATTATGAATTT
GAAGTTGTTTTAAATAAACCTGCTGCAAATCCAATAGCTTTATTTACTTTTCCTATTTTAGCCGAGCACCAATACAAGAG
CAAGGAAGATATTTTAAATAAAGACATCGTACCTATTGGTACAGGACCATATAAGATATCTTCTTACAGTGTTAGCAGAG
AGGTTATTTTTGAAAAAAATCCTTATTATAGAGGCGACAAACCCTATATAGATAATATAGTATTTAAAATTGTGCCTAAT
GAAAATGCCATGATAACATCTTTTCAAAGCAAAGAAGCAGATTTTACTTTTTTAAGTGACATAGACTGGGATAAATACAA
AGAGCTTTCAAATGTAAATATTTATAAATACGTTATGCAAGATTATGTATTTATGGCTCCTAATTATCAAAATCCAGCGT
TGAAAGATTCAAATGTCAGGAAAGCGATGTGTTATGGAATAGATACTGACAAAATTTTGAGAGATGTATATTTTGGTCAT
GGGTTAAAGTCATGCATCCCAGTCCGCCCTGATTCATGGCTTTATAGTAGTAAAATAGTAGCTCATAATTACGATATAAA
AGAGGCAAATAAAATATTAGAGGAAAATGGTTGGAATTTAGTTAAAGGGATAAGGAACAATGGAACTTATCAACTTAAAT
TTGATTTGATAGTAAATGTAAATAATCCTTATTTAATTAAGACTGCTCAAATTATAAAGAATAATTTAAAAAGCATAGGA
ATTGAAATAAAAATTGTACCAAAAGATTGGGATAATTTACTAAGTTCTGTGTATTCGGGTAAGTTTGATTTGGTGCTAAT
GGAGTGGAATTTAAGTTATAATCAAGATATGTCTGCGATGTTTATGACAAAAGGCAAAGATAATTTTATGGGTTATAGTA
ATTCTAAAGTTGATGAAATATATAGTAGAATTTTTTATGATTTAGAAGAAAATTCTTTAAAAGCAGATTATCAAGTTTTG
GAACAAGTTTTTTTAGAGGAACAACCTATAATTGGCCTTTTCTATATTGAAGGAGCAGTTATGGCATATGACAATGTAAA
AGGTGTGGACCCTACTGGATTTAATGTTTTCGACAATATAGAAAAATGGTATATAAAAAAGTAG

Upstream 100 bases:

>100_bases
GAAGTAGAAGGGGTACCTGTTGCTAAAAGAGGGAGAATTCCTGGTATTATAGAAAATAAAAAATTGAAAAAGATAAAATA
GTTTTCGGAGATGATAGTTA

Downstream 100 bases:

>100_bases
TATTAAAAAATAGGTATTGACACTGACCTAAAAAGATTATATAATTCATAGTGTGACGAAATTAAAAAAGCGTGCGGTGG
TGGCGGAACTGGCAGACGCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRKFIILSFILMFIFTGCKTTPQNMVNLPQASEDKTKIEEEKPLEGGTLRVNITSFDTLNPFLNSNERVRQMLNLSLEGL
VTLDKTLKPVPQLAEKWEINGLNIKFYLKKNVKWQDGVSFTANDVKFTFDSFKSKEVKSPYKDILINYLSSYKTNGDYEF
EVVLNKPAANPIALFTFPILAEHQYKSKEDILNKDIVPIGTGPYKISSYSVSREVIFEKNPYYRGDKPYIDNIVFKIVPN
ENAMITSFQSKEADFTFLSDIDWDKYKELSNVNIYKYVMQDYVFMAPNYQNPALKDSNVRKAMCYGIDTDKILRDVYFGH
GLKSCIPVRPDSWLYSSKIVAHNYDIKEANKILEENGWNLVKGIRNNGTYQLKFDLIVNVNNPYLIKTAQIIKNNLKSIG
IEIKIVPKDWDNLLSSVYSGKFDLVLMEWNLSYNQDMSAMFMTKGKDNFMGYSNSKVDEIYSRIFYDLEENSLKADYQVL
EQVFLEEQPIIGLFYIEGAVMAYDNVKGVDPTGFNVFDNIEKWYIKK

Sequences:

>Translated_527_residues
MRKFIILSFILMFIFTGCKTTPQNMVNLPQASEDKTKIEEEKPLEGGTLRVNITSFDTLNPFLNSNERVRQMLNLSLEGL
VTLDKTLKPVPQLAEKWEINGLNIKFYLKKNVKWQDGVSFTANDVKFTFDSFKSKEVKSPYKDILINYLSSYKTNGDYEF
EVVLNKPAANPIALFTFPILAEHQYKSKEDILNKDIVPIGTGPYKISSYSVSREVIFEKNPYYRGDKPYIDNIVFKIVPN
ENAMITSFQSKEADFTFLSDIDWDKYKELSNVNIYKYVMQDYVFMAPNYQNPALKDSNVRKAMCYGIDTDKILRDVYFGH
GLKSCIPVRPDSWLYSSKIVAHNYDIKEANKILEENGWNLVKGIRNNGTYQLKFDLIVNVNNPYLIKTAQIIKNNLKSIG
IEIKIVPKDWDNLLSSVYSGKFDLVLMEWNLSYNQDMSAMFMTKGKDNFMGYSNSKVDEIYSRIFYDLEENSLKADYQVL
EQVFLEEQPIIGLFYIEGAVMAYDNVKGVDPTGFNVFDNIEKWYIKK
>Mature_527_residues
MRKFIILSFILMFIFTGCKTTPQNMVNLPQASEDKTKIEEEKPLEGGTLRVNITSFDTLNPFLNSNERVRQMLNLSLEGL
VTLDKTLKPVPQLAEKWEINGLNIKFYLKKNVKWQDGVSFTANDVKFTFDSFKSKEVKSPYKDILINYLSSYKTNGDYEF
EVVLNKPAANPIALFTFPILAEHQYKSKEDILNKDIVPIGTGPYKISSYSVSREVIFEKNPYYRGDKPYIDNIVFKIVPN
ENAMITSFQSKEADFTFLSDIDWDKYKELSNVNIYKYVMQDYVFMAPNYQNPALKDSNVRKAMCYGIDTDKILRDVYFGH
GLKSCIPVRPDSWLYSSKIVAHNYDIKEANKILEENGWNLVKGIRNNGTYQLKFDLIVNVNNPYLIKTAQIIKNNLKSIG
IEIKIVPKDWDNLLSSVYSGKFDLVLMEWNLSYNQDMSAMFMTKGKDNFMGYSNSKVDEIYSRIFYDLEENSLKADYQVL
EQVFLEEQPIIGLFYIEGAVMAYDNVKGVDPTGFNVFDNIEKWYIKK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=479, Percent_Identity=26.3048016701461, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=23.6897274633124, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789887, Length=407, Percent_Identity=22.6044226044226, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787052, Length=493, Percent_Identity=24.1379310344828, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787762, Length=417, Percent_Identity=20.863309352518, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787551, Length=445, Percent_Identity=22.0224719101124, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789397, Length=473, Percent_Identity=22.6215644820296, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081878, Length=472, Percent_Identity=21.1864406779661, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61032; Mature: 61032

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFIILSFILMFIFTGCKTTPQNMVNLPQASEDKTKIEEEKPLEGGTLRVNITSFDTLN
CCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHCHHCCCCCCCEEEEEEECCCCCC
PFLNSNERVRQMLNLSLEGLVTLDKTLKPVPQLAEKWEINGLNIKFYLKKNVKWQDGVSF
HHHCCCHHHHHHHCCCHHHEEEEHHHHCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEE
TANDVKFTFDSFKSKEVKSPYKDILINYLSSYKTNGDYEFEVVLNKPAANPIALFTFPIL
EECCEEEEHHCCCCHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEHHH
AEHQYKSKEDILNKDIVPIGTGPYKISSYSVSREVIFEKNPYYRGDKPYIDNIVFKIVPN
HCCCCCCHHHHHCCCCEEECCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEEECC
ENAMITSFQSKEADFTFLSDIDWDKYKELSNVNIYKYVMQDYVFMAPNYQNPALKDSNVR
CCEEEEEECCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCEEEECCCCCCCCCCCCCCC
KAMCYGIDTDKILRDVYFGHGLKSCIPVRPDSWLYSSKIVAHNYDIKEANKILEENGWNL
EEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCEEECEEEEECCCHHHHHHHHHHCCCCE
VKGIRNNGTYQLKFDLIVNVNNPYLIKTAQIIKNNLKSIGIEIKIVPKDWDNLLSSVYSG
EHHHCCCCEEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCC
KFDLVLMEWNLSYNQDMSAMFMTKGKDNFMGYSNSKVDEIYSRIFYDLEENSLKADYQVL
CEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
EQVFLEEQPIIGLFYIEGAVMAYDNVKGVDPTGFNVFDNIEKWYIKK
HHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCHHHCCHHHEECC
>Mature Secondary Structure
MRKFIILSFILMFIFTGCKTTPQNMVNLPQASEDKTKIEEEKPLEGGTLRVNITSFDTLN
CCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHCHHCCCCCCCEEEEEEECCCCCC
PFLNSNERVRQMLNLSLEGLVTLDKTLKPVPQLAEKWEINGLNIKFYLKKNVKWQDGVSF
HHHCCCHHHHHHHCCCHHHEEEEHHHHCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEE
TANDVKFTFDSFKSKEVKSPYKDILINYLSSYKTNGDYEFEVVLNKPAANPIALFTFPIL
EECCEEEEHHCCCCHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEHHH
AEHQYKSKEDILNKDIVPIGTGPYKISSYSVSREVIFEKNPYYRGDKPYIDNIVFKIVPN
HCCCCCCHHHHHCCCCEEECCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEEECC
ENAMITSFQSKEADFTFLSDIDWDKYKELSNVNIYKYVMQDYVFMAPNYQNPALKDSNVR
CCEEEEEECCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCEEEECCCCCCCCCCCCCCC
KAMCYGIDTDKILRDVYFGHGLKSCIPVRPDSWLYSSKIVAHNYDIKEANKILEENGWNL
EEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCEEECEEEEECCCHHHHHHHHHHCCCCE
VKGIRNNGTYQLKFDLIVNVNNPYLIKTAQIIKNNLKSIGIEIKIVPKDWDNLLSSVYSG
EHHHCCCCEEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCC
KFDLVLMEWNLSYNQDMSAMFMTKGKDNFMGYSNSKVDEIYSRIFYDLEENSLKADYQVL
CEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
EQVFLEEQPIIGLFYIEGAVMAYDNVKGVDPTGFNVFDNIEKWYIKK
HHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCHHHCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]