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The map label for this gene is pgdS [H]

Identifier: 167040774

GI number: 167040774

Start: 2153575

End: 2154849

Strand: Reverse

Name: pgdS [H]

Synonym: Teth514_2151

Alternate gene names: 167040774

Gene position: 2154849-2153575 (Counterclockwise)

Preceding gene: 167040775

Following gene: 167040773

Centisome position: 87.69

GC content: 37.96

Gene sequence:

>1275_bases
ATGCAATCTTGCCGAATCCGAAAGGTACGGAGGAACCGCTTTTTGGGGTTAATCTGCAGTGAAGCTGCAGTAGGGATACC
TTCTGTCCCGCACCCGACAGCTAACTCCGGAGGCAATAAAGGAAGGAGATTTGCAGTGGATCAGCGAATAGGAAAGATGA
TTTTTGGAATTTCTGTGTTTGGCGCGACGCTGATTGGTAGTTCTTTTTTGAACCCAGCGTTTGCAGAAGGATTGGGAGTT
GGAAAGATTACGGGAAATTATGTAAATGTACGAACGCAGGGCAGTTTATCAGGAAGCATTATTACACGGCTAAATTTAAA
TGATACTGTGACTGTGTTAGATCAGCAAAATGGATGGTACAAGATTAAGCTTTCAGATGGAAAAGAAGGTTGGGTTTTTG
GAGAGTATTTAGCATTAGTTAATGGCCAAAATGCACCGGTAACAGGAGTTGGTACTGTGACCGGTAGTAGAGTGAACGTA
AGAAGTGCAGCCAGTTTATCTGCAAGCATAATAACACAACTTGCAAAAAATACTGTAGTAGATGTTTTAGGAAAGCAAAA
TGATTGGTACAAAGTAAGACTTTCTAACAACAAAGAAGGATGGATTTACAGTCAATATTTAGCAGTAAAAAGTGTAGATA
CTACTGTATCAAGAGGAAGTGTTAATAGAACACCAATAGCTGTTGGCATAGTAACAGGTAGTGTAGTAAATGTGAGAAGT
GCAGGTAATATATCTGCCAATGTGATAGCACAAGTTACAAAAAATACAAAAGTGGATGTATTAGGAAATCAAAATGGATG
GTACAATATAAGGCTTTCAGATGGAAGAGAAGGTTGGATATATGGCCAATATTTATCAGTAGGAACACAAACGATTGTAT
CAAGAGGAGACGTAGATAGAAGTGTGGTAAACAAGCTTATAGAATTTGCTAAATCTCTTTTGGGAACCAAGTATGTGTAT
GGAGGTTCATCTCCTGCAGGGTTTGATTGTTCCGGTTTTGTTCAATATGTTTTTAAGAATTTTGATATTAATTTACCAAG
AACGGCGAAGGACCAATCTACTGTAGGAGAATATGTAAGTTATAGCAACCTTCAACCAGGAGATTTGGTATTTTTTAAAA
CTTTGGGAAGTAGTGTGATAAATCACTCAGGAATATATATAGGCAATGGTGAGTTTATACACAGCTCTTCAGGGGCTGGA
AAAGTGATAATAAGTAATATAACTTCGGGATATTATAAAGACCATTATACTACAGCACGACGCGTTATAAGATAA

Upstream 100 bases:

>100_bases
TATAAAATATATTAAAAAAAGATTAAAAAGTTTAAGAAAATGTAATGTAAACATTGACTTTGACTGTAGGATATGATAAG
ATAAGACAAAAGGACAAAAT

Downstream 100 bases:

>100_bases
GCATAAAAAAAGAGTACTCCAAGGAGTGCTCTTTTTTATATTGACTATTTTTATTCCTTGATATATCATATAATACTGTA
TACGCGATATAGCCTATTTT

Product: NLP/P60 protein

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MQSCRIRKVRRNRFLGLICSEAAVGIPSVPHPTANSGGNKGRRFAVDQRIGKMIFGISVFGATLIGSSFLNPAFAEGLGV
GKITGNYVNVRTQGSLSGSIITRLNLNDTVTVLDQQNGWYKIKLSDGKEGWVFGEYLALVNGQNAPVTGVGTVTGSRVNV
RSAASLSASIITQLAKNTVVDVLGKQNDWYKVRLSNNKEGWIYSQYLAVKSVDTTVSRGSVNRTPIAVGIVTGSVVNVRS
AGNISANVIAQVTKNTKVDVLGNQNGWYNIRLSDGREGWIYGQYLSVGTQTIVSRGDVDRSVVNKLIEFAKSLLGTKYVY
GGSSPAGFDCSGFVQYVFKNFDINLPRTAKDQSTVGEYVSYSNLQPGDLVFFKTLGSSVINHSGIYIGNGEFIHSSSGAG
KVIISNITSGYYKDHYTTARRVIR

Sequences:

>Translated_424_residues
MQSCRIRKVRRNRFLGLICSEAAVGIPSVPHPTANSGGNKGRRFAVDQRIGKMIFGISVFGATLIGSSFLNPAFAEGLGV
GKITGNYVNVRTQGSLSGSIITRLNLNDTVTVLDQQNGWYKIKLSDGKEGWVFGEYLALVNGQNAPVTGVGTVTGSRVNV
RSAASLSASIITQLAKNTVVDVLGKQNDWYKVRLSNNKEGWIYSQYLAVKSVDTTVSRGSVNRTPIAVGIVTGSVVNVRS
AGNISANVIAQVTKNTKVDVLGNQNGWYNIRLSDGREGWIYGQYLSVGTQTIVSRGDVDRSVVNKLIEFAKSLLGTKYVY
GGSSPAGFDCSGFVQYVFKNFDINLPRTAKDQSTVGEYVSYSNLQPGDLVFFKTLGSSVINHSGIYIGNGEFIHSSSGAG
KVIISNITSGYYKDHYTTARRVIR
>Mature_424_residues
MQSCRIRKVRRNRFLGLICSEAAVGIPSVPHPTANSGGNKGRRFAVDQRIGKMIFGISVFGATLIGSSFLNPAFAEGLGV
GKITGNYVNVRTQGSLSGSIITRLNLNDTVTVLDQQNGWYKIKLSDGKEGWVFGEYLALVNGQNAPVTGVGTVTGSRVNV
RSAASLSASIITQLAKNTVVDVLGKQNDWYKVRLSNNKEGWIYSQYLAVKSVDTTVSRGSVNRTPIAVGIVTGSVVNVRS
AGNISANVIAQVTKNTKVDVLGNQNGWYNIRLSDGREGWIYGQYLSVGTQTIVSRGDVDRSVVNKLIEFAKSLLGTKYVY
GGSSPAGFDCSGFVQYVFKNFDINLPRTAKDQSTVGEYVSYSNLQPGDLVFFKTLGSSVINHSGIYIGNGEFIHSSSGAG
KVIISNITSGYYKDHYTTARRVIR

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=135, Percent_Identity=41.4814814814815, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787944, Length=126, Percent_Identity=40.4761904761905, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788001, Length=123, Percent_Identity=40.650406504065, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786421, Length=114, Percent_Identity=37.719298245614, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 45717; Mature: 45717

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSCRIRKVRRNRFLGLICSEAAVGIPSVPHPTANSGGNKGRRFAVDQRIGKMIFGISVF
CCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
GATLIGSSFLNPAFAEGLGVGKITGNYVNVRTQGSLSGSIITRLNLNDTVTVLDQQNGWY
HHHHHHHHHCCHHHHHCCCCCEEECCEEEEEECCCCCCEEEEEEECCCEEEEEECCCCEE
KIKLSDGKEGWVFGEYLALVNGQNAPVTGVGTVTGSRVNVRSAASLSASIITQLAKNTVV
EEEECCCCCCCEEEEEEEEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHH
DVLGKQNDWYKVRLSNNKEGWIYSQYLAVKSVDTTVSRGSVNRTPIAVGIVTGSVVNVRS
HHHCCCCCEEEEEECCCCCCEEEEEEEEEEECCCHHCCCCCCCCCEEEEEEECCEEEEEC
AGNISANVIAQVTKNTKVDVLGNQNGWYNIRLSDGREGWIYGQYLSVGTQTIVSRGDVDR
CCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCEEEEEHHHHCHHHHHHCCCCCH
SVVNKLIEFAKSLLGTKYVYGGSSPAGFDCSGFVQYVFKNFDINLPRTAKDQSTVGEYVS
HHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
YSNLQPGDLVFFKTLGSSVINHSGIYIGNGEFIHSSSGAGKVIISNITSGYYKDHYTTAR
CCCCCCCCEEEEEHHHHHHHCCCCEEECCCCEEECCCCCCEEEEECCCCCHHHHHHHHHH
RVIR
HHCC
>Mature Secondary Structure
MQSCRIRKVRRNRFLGLICSEAAVGIPSVPHPTANSGGNKGRRFAVDQRIGKMIFGISVF
CCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
GATLIGSSFLNPAFAEGLGVGKITGNYVNVRTQGSLSGSIITRLNLNDTVTVLDQQNGWY
HHHHHHHHHCCHHHHHCCCCCEEECCEEEEEECCCCCCEEEEEEECCCEEEEEECCCCEE
KIKLSDGKEGWVFGEYLALVNGQNAPVTGVGTVTGSRVNVRSAASLSASIITQLAKNTVV
EEEECCCCCCCEEEEEEEEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHH
DVLGKQNDWYKVRLSNNKEGWIYSQYLAVKSVDTTVSRGSVNRTPIAVGIVTGSVVNVRS
HHHCCCCCEEEEEECCCCCCEEEEEEEEEEECCCHHCCCCCCCCCEEEEEEECCEEEEEC
AGNISANVIAQVTKNTKVDVLGNQNGWYNIRLSDGREGWIYGQYLSVGTQTIVSRGDVDR
CCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCEEEEEHHHHCHHHHHHCCCCCH
SVVNKLIEFAKSLLGTKYVYGGSSPAGFDCSGFVQYVFKNFDINLPRTAKDQSTVGEYVS
HHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
YSNLQPGDLVFFKTLGSSVINHSGIYIGNGEFIHSSSGAGKVIISNITSGYYKDHYTTAR
CCCCCCCCEEEEEHHHHHHHCCCCEEECCCCEEECCCCCCEEEEECCCCCHHHHHHHHHH
RVIR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]