Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 167040728

GI number: 167040728

Start: 2113315

End: 2114580

Strand: Direct

Name: dacB [H]

Synonym: Teth514_2105

Alternate gene names: 167040728

Gene position: 2113315-2114580 (Clockwise)

Preceding gene: 167040726

Following gene: 167040742

Centisome position: 86.0

GC content: 33.81

Gene sequence:

>1266_bases
ATGAAAAAGATAATAGCTTCTTTGCTCACACTATTTATTTTGGTAGTGCAAATAACTCCTGTATACGCTAATGTGCCACC
ACCACCTGAAATTGTAGGCCCAACTGCAGTTTTAATGGATTTTACAACAGGTCAAGTATTGTATGACAAAAATATGCACA
AAAGAATGTACCCCGCAAGCACAACTAAAATTCTTACTGCAATTATTGCTATAGAAAAGGGAAACTTAAACGATATTGTA
ACTGCTAGCAATAATGTTACAAAAATAGATGGAAATTCCATATTTCTTTCTCCTGGTGAGCAACTTACGTTAGAACAATT
ACTTTATGCGATTATGTTACAATCTGCCAATGATGCAGCAATAGCAATTGCTGAACACATTGGTGGAAGCGTAGAAAACT
TTGCTAAAATGATGAATGAAAAAGCTAAAGAAATTGGAGCTTATGATAGCCATTTTGTAAATCCAAATGGTCTTCCTGAT
GACAATCATTATTCCACCCCTTATGATATGGCATTAATAGCAAGGTATGCAATGCAAAATCCAGAATTTAGAAAAATAGT
AACTACAATACATTATCAAATTCCTCCTACTAATAAGTTTGATAAAGTAAGAGACCTATGGTTAAGCAATCGCTTAATTA
AACCTTCAAGTTTTCGCTATGAAGGAGCTGATGGAGTAAAAACAGGAGACACGGTTGCAGCTGGTCAAGTTTTAGCAGCT
TCTGCAACAAGAGATGGTCGCAGACTTATATCCGTCATAATGGGAGACCAAGGAACAAATATCTGGACAGATACTATAAA
ACTTTTGGACTACGGGTTTCAAAACTTTAGTTTAGTGAAAAAAACAGAAAAGGGTGAAACAATAACATATATCGACTTAG
GAAAGTCCCACTTTAAACTTCCTCTTATTGCAAAAGAAGACTTTTACTACGAAGTTCCTAAAGGTCAAGAAAATTCTATA
GAGACCCAAATAATTCTAAATAAGGATATCAAAACGCCAATAAATAAAGGAGATGTGTTAGGGTACATCAAATATACTTT
AGATGGCAACGAAATTGGTACAAATCCTCTCATTGCAGCCGAATCTGTTTATAAAAACTTTTGGGGAAACTATTATACGG
AGGTACAATCTTCTGGTGTAAAAAATACAATGTATACTAACCCCGTAAAATTTATTGCTGGAACAACAATTTTAGGAATA
GCTTTTATCCTTTGGAGAAGACGGAAAATTAGAAATAGAAAAAAATATATCTTCTCAAAATATTGA

Upstream 100 bases:

>100_bases
TTACAAGCTCCTTTATTTCTACATAAATCAAAAAATTCCTTTTTATTTTTCGCGTTTTTTTGTTATACTTTAAGTATCAG
CATAAATTGGAGGCATAAAA

Downstream 100 bases:

>100_bases
TTTAAAAAGAGGGCAGAATGCCCTCTTTTTATGCATATATTTGGTTGTCATTGAGGCTTCTACTTCTTCCCATGTCCTTT
ATAAAAGAATCAACATTAAA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKKIIASLLTLFILVVQITPVYANVPPPPEIVGPTAVLMDFTTGQVLYDKNMHKRMYPASTTKILTAIIAIEKGNLNDIV
TASNNVTKIDGNSIFLSPGEQLTLEQLLYAIMLQSANDAAIAIAEHIGGSVENFAKMMNEKAKEIGAYDSHFVNPNGLPD
DNHYSTPYDMALIARYAMQNPEFRKIVTTIHYQIPPTNKFDKVRDLWLSNRLIKPSSFRYEGADGVKTGDTVAAGQVLAA
SATRDGRRLISVIMGDQGTNIWTDTIKLLDYGFQNFSLVKKTEKGETITYIDLGKSHFKLPLIAKEDFYYEVPKGQENSI
ETQIILNKDIKTPINKGDVLGYIKYTLDGNEIGTNPLIAAESVYKNFWGNYYTEVQSSGVKNTMYTNPVKFIAGTTILGI
AFILWRRRKIRNRKKYIFSKY

Sequences:

>Translated_421_residues
MKKIIASLLTLFILVVQITPVYANVPPPPEIVGPTAVLMDFTTGQVLYDKNMHKRMYPASTTKILTAIIAIEKGNLNDIV
TASNNVTKIDGNSIFLSPGEQLTLEQLLYAIMLQSANDAAIAIAEHIGGSVENFAKMMNEKAKEIGAYDSHFVNPNGLPD
DNHYSTPYDMALIARYAMQNPEFRKIVTTIHYQIPPTNKFDKVRDLWLSNRLIKPSSFRYEGADGVKTGDTVAAGQVLAA
SATRDGRRLISVIMGDQGTNIWTDTIKLLDYGFQNFSLVKKTEKGETITYIDLGKSHFKLPLIAKEDFYYEVPKGQENSI
ETQIILNKDIKTPINKGDVLGYIKYTLDGNEIGTNPLIAAESVYKNFWGNYYTEVQSSGVKNTMYTNPVKFIAGTTILGI
AFILWRRRKIRNRKKYIFSKY
>Mature_421_residues
MKKIIASLLTLFILVVQITPVYANVPPPPEIVGPTAVLMDFTTGQVLYDKNMHKRMYPASTTKILTAIIAIEKGNLNDIV
TASNNVTKIDGNSIFLSPGEQLTLEQLLYAIMLQSANDAAIAIAEHIGGSVENFAKMMNEKAKEIGAYDSHFVNPNGLPD
DNHYSTPYDMALIARYAMQNPEFRKIVTTIHYQIPPTNKFDKVRDLWLSNRLIKPSSFRYEGADGVKTGDTVAAGQVLAA
SATRDGRRLISVIMGDQGTNIWTDTIKLLDYGFQNFSLVKKTEKGETITYIDLGKSHFKLPLIAKEDFYYEVPKGQENSI
ETQIILNKDIKTPINKGDVLGYIKYTLDGNEIGTNPLIAAESVYKNFWGNYYTEVQSSGVKNTMYTNPVKFIAGTTILGI
AFILWRRRKIRNRKKYIFSKY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=360, Percent_Identity=31.6666666666667, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786851, Length=385, Percent_Identity=30.6493506493506, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082022, Length=390, Percent_Identity=30.2564102564103, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87082054, Length=242, Percent_Identity=26.0330578512397, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47181; Mature: 47181

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIASLLTLFILVVQITPVYANVPPPPEIVGPTAVLMDFTTGQVLYDKNMHKRMYPAS
CHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHCCCCH
TTKILTAIIAIEKGNLNDIVTASNNVTKIDGNSIFLSPGEQLTLEQLLYAIMLQSANDAA
HHHHHHHHEEEECCCCCCEEECCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCE
IAIAEHIGGSVENFAKMMNEKAKEIGAYDSHFVNPNGLPDDNHYSTPYDMALIARYAMQN
EEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PEFRKIVTTIHYQIPPTNKFDKVRDLWLSNRLIKPSSFRYEGADGVKTGDTVAAGQVLAA
CHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEE
SATRDGRRLISVIMGDQGTNIWTDTIKLLDYGFQNFSLVKKTEKGETITYIDLGKSHFKL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCEEE
PLIAKEDFYYEVPKGQENSIETQIILNKDIKTPINKGDVLGYIKYTLDGNEIGTNPLIAA
EEEECCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHH
ESVYKNFWGNYYTEVQSSGVKNTMYTNPVKFIAGTTILGIAFILWRRRKIRNRKKYIFSK
HHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
Y
C
>Mature Secondary Structure
MKKIIASLLTLFILVVQITPVYANVPPPPEIVGPTAVLMDFTTGQVLYDKNMHKRMYPAS
CHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHCCCCH
TTKILTAIIAIEKGNLNDIVTASNNVTKIDGNSIFLSPGEQLTLEQLLYAIMLQSANDAA
HHHHHHHHEEEECCCCCCEEECCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCE
IAIAEHIGGSVENFAKMMNEKAKEIGAYDSHFVNPNGLPDDNHYSTPYDMALIARYAMQN
EEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PEFRKIVTTIHYQIPPTNKFDKVRDLWLSNRLIKPSSFRYEGADGVKTGDTVAAGQVLAA
CHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEE
SATRDGRRLISVIMGDQGTNIWTDTIKLLDYGFQNFSLVKKTEKGETITYIDLGKSHFKL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCEEE
PLIAKEDFYYEVPKGQENSIETQIILNKDIKTPINKGDVLGYIKYTLDGNEIGTNPLIAA
EEEECCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHH
ESVYKNFWGNYYTEVQSSGVKNTMYTNPVKFIAGTTILGIAFILWRRRKIRNRKKYIFSK
HHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]