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The map label for this gene is yqjC [H]

Identifier: 167040483

GI number: 167040483

Start: 1868689

End: 1869090

Strand: Reverse

Name: yqjC [H]

Synonym: Teth514_1852

Alternate gene names: 167040483

Gene position: 1869090-1868689 (Counterclockwise)

Preceding gene: 167040484

Following gene: 167040482

Centisome position: 76.06

GC content: 47.76

Gene sequence:

>402_bases
ATGAATATCCTGAAAATAGACCACATAGGCATAGCCGTGAACAGCATAGAAGAGGCATCCAAAATATACACAGAACTTCT
GGGACTTGAGATGCACGGTGTGGAGGAAGTGGCGGAGCAGAAAGTAAAAGTGGCCTTCATACCGGTTGGGGAAAGCGAAG
TGGAACTTTTAGAATCCACCGATCCCGAAGGCCCCATCGCACGGTTCATAGAAAAAAATGGTGAGGGTGTACAGCACATA
GCCTTCCGGGTGGAGGACATCGAAAAAGCTCTAGAAGAACTCAAGCAAAAGGGTGTAAGGCTCATCGACGAAAAACCAAG
GTATGGTGCCGGGGGTGCAAAAATCGCCTTCATCCACCCCAAAGCCACAAAAGGCGTTCTCATAGAATTATGCGAGAGAT
AA

Upstream 100 bases:

>100_bases
AAAATAGACATGCTGTTACAGGATATATCCAAGAAGGCGGTTGATCCGTATACGGCCCTTGATATTCTTACAAGAGAAAT
CCTTTAGGAGGTAAAAAGCC

Downstream 100 bases:

>100_bases
AAGGGGAGTGAAAGGGCAAAAGAAGGGGATGAAAAAATGATAGAAGCATTAAGTACCACTGTTTTAGGCTTTGGAATAAC
CCTCATCTTCCTTTACCTGC

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MNILKIDHIGIAVNSIEEASKIYTELLGLEMHGVEEVAEQKVKVAFIPVGESEVELLESTDPEGPIARFIEKNGEGVQHI
AFRVEDIEKALEELKQKGVRLIDEKPRYGAGGAKIAFIHPKATKGVLIELCER

Sequences:

>Translated_133_residues
MNILKIDHIGIAVNSIEEASKIYTELLGLEMHGVEEVAEQKVKVAFIPVGESEVELLESTDPEGPIARFIEKNGEGVQHI
AFRVEDIEKALEELKQKGVRLIDEKPRYGAGGAKIAFIHPKATKGVLIELCER
>Mature_133_residues
MNILKIDHIGIAVNSIEEASKIYTELLGLEMHGVEEVAEQKVKVAFIPVGESEVELLESTDPEGPIARFIEKNGEGVQHI
AFRVEDIEKALEELKQKGVRLIDEKPRYGAGGAKIAFIHPKATKGVLIELCER

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=131, Percent_Identity=35.8778625954198, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506387, Length=127, Percent_Identity=37.007874015748, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14701; Mature: 14701

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILKIDHIGIAVNSIEEASKIYTELLGLEMHGVEEVAEQKVKVAFIPVGESEVELLEST
CCEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCHHHHHHHCC
DPEGPIARFIEKNGEGVQHIAFRVEDIEKALEELKQKGVRLIDEKPRYGAGGAKIAFIHP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEECC
KATKGVLIELCER
CCCHHHHHHHHCC
>Mature Secondary Structure
MNILKIDHIGIAVNSIEEASKIYTELLGLEMHGVEEVAEQKVKVAFIPVGESEVELLEST
CCEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCHHHHHHHCC
DPEGPIARFIEKNGEGVQHIAFRVEDIEKALEELKQKGVRLIDEKPRYGAGGAKIAFIHP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEECC
KATKGVLIELCER
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]