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The map label for this gene is topA [H]

Identifier: 167040337

GI number: 167040337

Start: 1709588

End: 1711669

Strand: Reverse

Name: topA [H]

Synonym: Teth514_1700

Alternate gene names: 167040337

Gene position: 1711669-1709588 (Counterclockwise)

Preceding gene: 167040338

Following gene: 167040336

Centisome position: 69.66

GC content: 35.59

Gene sequence:

>2082_bases
ATGGCAAAATCTCTCGTTATTGTGGAATCTCCAGCTAAAGCTAAGACCATCTCTAAATTTTTAGGCAAAAATTTTAAAGT
AGCAGCCTCCATGGGACATGTTAGGGATTTGCCTAAAAGTCAATTAGGTATCGATATTGAAAATAATTTTGCTCCTAAGT
ACATAACTATCAGGGGAAAAGGGGCTATTATAGAGAATTTAAAAAAAGAAGCAAAGGATGCAGATAAAATTTTTTTGGCC
ACTGACCCTGACAGAGAAGGAGAAGCAATTTCCTGGCATATTGCCCAATTGTTAAATTTGAATATTGAGGAGCCTTGTCG
CATAGAATTTCACGAGATTACAAAAAATGCAGTGCAAACTGCTCTTAAAAGCCCTCGCTCTATAGATTTAAATTTGGTAG
ATGCGCAACAAGCCAGGAGAATTCTTGATAGATTGGTAGGATATAAAATAAGTCCCCTTCTTTGGAAAAAAATAAAGAGG
GGACTAAGTGCAGGCAGAGTTCAATCAGTAGCAGCTAGGCTTATCTGCGACAGAGAAAGAGAAATAGAAGCCTTTGTGCC
AGAAGAATATTGGAGTATTACCGCGATTCTCTCTGATGAGAAAAACTCTGGCAAGTTTGAGGCAAAATTCTACGGTACCA
GAGAGGATAAAGTTGAGTTAAAGACAAAAGAAGATGTAGACAAAATATTAGCAGCATTGTCTGAAGAATACATTATAGAT
AAAGTAAAAACAGGTGTAAAAAAGAGGAATCCTTCTCCACCTTTTATCACTAGTACTTTGCAACAAGAAGCTTCGAGAAA
ATTGGGATTTACTGCAAAAAAAACTATGTTAATCGCACAACAATTATATGAAGGCGTAGAAATCAAAGGAGAAGGCAGTG
TAGGTCTTATTACTTATATAAGGACTGATTCTACAAGAGTCGCAAATGAAGCTAAGGCTGAAGTCTATAAATACATAGTT
GAAAAGTTTGGAAAAGAGTATACCAATCCTGAAGCAAATTATGCTTCTAAAAGAGCCAATGTACAGGATGCTCACGAGGC
TATAAGACCTACTTCTATTTATAGGGATCCGGAAAGTATAAAAGATTCTCTAACGCCAGACCAATATAAATTGTACAAAC
TAGTTTGGAGTAGATTCCTAGCAAGTCAAATGGCACCTGCGATTTACGATACTGTTTCTATGGATATAGTAAATAATGGA
TATGTTTTTAAAGCCTCCGGTTCTAGCATTAAATTTTTAGGTTTTATGACAGTGTATGTAGAAGGAACAGATATGCAAGA
AGAGGAAGAAAAAACTGTACCTGTGCTTCAAGAAGGAACCAAATTGTTTTTAGAGGAATTAAAGCCAATGCAACATTTTA
CCCAGCCTCCGCCTCGATATACAGAAGCTTCTTTGATTAAAGAATTAGAAGAAAAAGGAATAGGCCGTCCCAGTACTTAT
GCTCCTACTATCTCTACTTTGTTAGAAAGAGGATATGTGGTAAAAGATAAAAAGAATTTAAAACCTACAGAATTAGGTTT
TATTGTGACAGACGCATTAAAGGAGTTCTTTTCCAAAATTGTAGATGTAAAATTTACTGCTGAGATGGAAGAGCAGCTGG
ATAAGATTGAAGAAGGTCAAGTGAAGTGGTATGAAGTGGTGCAAGAGTTTTATGAAGACTTTTATAATACTTTGAAAATT
GCTGAAGAACAAATGGAAGAGATAGACGTAAAGGAAGAAGTAGAAGTAACTGACATTAAATGTGAACTTTGCGGGAGAAA
TATGGTTGTAAAGAAAGGCAGGTATGGAAAATTTCTTGCTTGTTCTGGTTTTCCTGAATGTAAAAATACAAAACCTTTGT
ACGAAAAGGTTGGTGTGAAATGCCCTAAATGTGGTGGAGAAATTGTAAAGAAAAAGAGCAAAAAAGGGCGAACGTATTAC
GCTTGCGAAAATGCACCTAATTGCGATTTCATATTGTGGGATAAGCCCGTTGATGAAAAATGTCCTGTTTGTGGTAGCTT
GTTAGTAGAAAAAAATACAAAAAATGGTCATATACTAAAATGCTCTAATCCAGAATGTGATTATCAAAAAGAAGTTAAAT
GA

Upstream 100 bases:

>100_bases
GTATGATTGAAAAACTTCCTGGCAATAAATATGAGAAAAAAATATTTTGATAATTGTCAAAAACTTTGATATAATTATAA
ATTGTTTTGGAGGTGTCGAA

Downstream 100 bases:

>100_bases
ATTTAACAAAAATTTTGAATTAATTCTTGAAGCATTGTGAAAAATGTGTTAATATTAAACTATCTAAAGGGGTGTAAACA
TGTTTAAGGGTACTACCATA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MAKSLVIVESPAKAKTISKFLGKNFKVAASMGHVRDLPKSQLGIDIENNFAPKYITIRGKGAIIENLKKEAKDADKIFLA
TDPDREGEAISWHIAQLLNLNIEEPCRIEFHEITKNAVQTALKSPRSIDLNLVDAQQARRILDRLVGYKISPLLWKKIKR
GLSAGRVQSVAARLICDREREIEAFVPEEYWSITAILSDEKNSGKFEAKFYGTREDKVELKTKEDVDKILAALSEEYIID
KVKTGVKKRNPSPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGVEIKGEGSVGLITYIRTDSTRVANEAKAEVYKYIV
EKFGKEYTNPEANYASKRANVQDAHEAIRPTSIYRDPESIKDSLTPDQYKLYKLVWSRFLASQMAPAIYDTVSMDIVNNG
YVFKASGSSIKFLGFMTVYVEGTDMQEEEEKTVPVLQEGTKLFLEELKPMQHFTQPPPRYTEASLIKELEEKGIGRPSTY
APTISTLLERGYVVKDKKNLKPTELGFIVTDALKEFFSKIVDVKFTAEMEEQLDKIEEGQVKWYEVVQEFYEDFYNTLKI
AEEQMEEIDVKEEVEVTDIKCELCGRNMVVKKGRYGKFLACSGFPECKNTKPLYEKVGVKCPKCGGEIVKKKSKKGRTYY
ACENAPNCDFILWDKPVDEKCPVCGSLLVEKNTKNGHILKCSNPECDYQKEVK

Sequences:

>Translated_693_residues
MAKSLVIVESPAKAKTISKFLGKNFKVAASMGHVRDLPKSQLGIDIENNFAPKYITIRGKGAIIENLKKEAKDADKIFLA
TDPDREGEAISWHIAQLLNLNIEEPCRIEFHEITKNAVQTALKSPRSIDLNLVDAQQARRILDRLVGYKISPLLWKKIKR
GLSAGRVQSVAARLICDREREIEAFVPEEYWSITAILSDEKNSGKFEAKFYGTREDKVELKTKEDVDKILAALSEEYIID
KVKTGVKKRNPSPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGVEIKGEGSVGLITYIRTDSTRVANEAKAEVYKYIV
EKFGKEYTNPEANYASKRANVQDAHEAIRPTSIYRDPESIKDSLTPDQYKLYKLVWSRFLASQMAPAIYDTVSMDIVNNG
YVFKASGSSIKFLGFMTVYVEGTDMQEEEEKTVPVLQEGTKLFLEELKPMQHFTQPPPRYTEASLIKELEEKGIGRPSTY
APTISTLLERGYVVKDKKNLKPTELGFIVTDALKEFFSKIVDVKFTAEMEEQLDKIEEGQVKWYEVVQEFYEDFYNTLKI
AEEQMEEIDVKEEVEVTDIKCELCGRNMVVKKGRYGKFLACSGFPECKNTKPLYEKVGVKCPKCGGEIVKKKSKKGRTYY
ACENAPNCDFILWDKPVDEKCPVCGSLLVEKNTKNGHILKCSNPECDYQKEVK
>Mature_692_residues
AKSLVIVESPAKAKTISKFLGKNFKVAASMGHVRDLPKSQLGIDIENNFAPKYITIRGKGAIIENLKKEAKDADKIFLAT
DPDREGEAISWHIAQLLNLNIEEPCRIEFHEITKNAVQTALKSPRSIDLNLVDAQQARRILDRLVGYKISPLLWKKIKRG
LSAGRVQSVAARLICDREREIEAFVPEEYWSITAILSDEKNSGKFEAKFYGTREDKVELKTKEDVDKILAALSEEYIIDK
VKTGVKKRNPSPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGVEIKGEGSVGLITYIRTDSTRVANEAKAEVYKYIVE
KFGKEYTNPEANYASKRANVQDAHEAIRPTSIYRDPESIKDSLTPDQYKLYKLVWSRFLASQMAPAIYDTVSMDIVNNGY
VFKASGSSIKFLGFMTVYVEGTDMQEEEEKTVPVLQEGTKLFLEELKPMQHFTQPPPRYTEASLIKELEEKGIGRPSTYA
PTISTLLERGYVVKDKKNLKPTELGFIVTDALKEFFSKIVDVKFTAEMEEQLDKIEEGQVKWYEVVQEFYEDFYNTLKIA
EEQMEEIDVKEEVEVTDIKCELCGRNMVVKKGRYGKFLACSGFPECKNTKPLYEKVGVKCPKCGGEIVKKKSKKGRTYYA
CENAPNCDFILWDKPVDEKCPVCGSLLVEKNTKNGHILKCSNPECDYQKEVK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=631, Percent_Identity=26.6244057052298, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI4507635, Length=623, Percent_Identity=23.2744783306581, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1787529, Length=762, Percent_Identity=39.8950131233596, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788061, Length=542, Percent_Identity=23.9852398523985, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI48994931, Length=112, Percent_Identity=36.6071428571429, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17555378, Length=518, Percent_Identity=26.4478764478764, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=561, Percent_Identity=24.9554367201426, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=554, Percent_Identity=25.4512635379061, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585251, Length=640, Percent_Identity=25.9375, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640096, Length=652, Percent_Identity=21.0122699386503, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78634; Mature: 78503

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSLVIVESPAKAKTISKFLGKNFKVAASMGHVRDLPKSQLGIDIENNFAPKYITIRGK
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHCCCHHHCCEEECCCCCCCEEEEECC
GAIIENLKKEAKDADKIFLATDPDREGEAISWHIAQLLNLNIEEPCRIEFHEITKNAVQT
CHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ALKSPRSIDLNLVDAQQARRILDRLVGYKISPLLWKKIKRGLSAGRVQSVAARLICDRER
HHCCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EIEAFVPEEYWSITAILSDEKNSGKFEAKFYGTREDKVELKTKEDVDKILAALSEEYIID
CHHHCCCHHHEEEEEEEECCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHH
KVKTGVKKRNPSPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGVEIKGEGSVGLITYI
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEEE
RTDSTRVANEAKAEVYKYIVEKFGKEYTNPEANYASKRANVQDAHEAIRPTSIYRDPESI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCCHHHCCCHHHH
KDSLTPDQYKLYKLVWSRFLASQMAPAIYDTVSMDIVNNGYVFKASGSSIKFLGFMTVYV
HHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHEEEEECCCEEEEECCCCEEEEEEEEEEE
EGTDMQEEEEKTVPVLQEGTKLFLEELKPMQHFTQPPPRYTEASLIKELEEKGIGRPSTY
ECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
APTISTLLERGYVVKDKKNLKPTELGFIVTDALKEFFSKIVDVKFTAEMEEQLDKIEEGQ
CHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCC
VKWYEVVQEFYEDFYNTLKIAEEQMEEIDVKEEVEVTDIKCELCGRNMVVKKGRYGKFLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEEEEECCCCEEEECCCCCCEEE
CSGFPECKNTKPLYEKVGVKCPKCGGEIVKKKSKKGRTYYACENAPNCDFILWDKPVDEK
ECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCC
CPVCGSLLVEKNTKNGHILKCSNPECDYQKEVK
CCCCCHHEEECCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKSLVIVESPAKAKTISKFLGKNFKVAASMGHVRDLPKSQLGIDIENNFAPKYITIRGK
CCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHCCCHHHCCEEECCCCCCCEEEEECC
GAIIENLKKEAKDADKIFLATDPDREGEAISWHIAQLLNLNIEEPCRIEFHEITKNAVQT
CHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ALKSPRSIDLNLVDAQQARRILDRLVGYKISPLLWKKIKRGLSAGRVQSVAARLICDRER
HHCCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EIEAFVPEEYWSITAILSDEKNSGKFEAKFYGTREDKVELKTKEDVDKILAALSEEYIID
CHHHCCCHHHEEEEEEEECCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHH
KVKTGVKKRNPSPPFITSTLQQEASRKLGFTAKKTMLIAQQLYEGVEIKGEGSVGLITYI
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEEE
RTDSTRVANEAKAEVYKYIVEKFGKEYTNPEANYASKRANVQDAHEAIRPTSIYRDPESI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCCHHHCCCHHHH
KDSLTPDQYKLYKLVWSRFLASQMAPAIYDTVSMDIVNNGYVFKASGSSIKFLGFMTVYV
HHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHEEEEECCCEEEEECCCCEEEEEEEEEEE
EGTDMQEEEEKTVPVLQEGTKLFLEELKPMQHFTQPPPRYTEASLIKELEEKGIGRPSTY
ECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
APTISTLLERGYVVKDKKNLKPTELGFIVTDALKEFFSKIVDVKFTAEMEEQLDKIEEGQ
CHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCC
VKWYEVVQEFYEDFYNTLKIAEEQMEEIDVKEEVEVTDIKCELCGRNMVVKKGRYGKFLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEEEEECCCCEEEECCCCCCEEE
CSGFPECKNTKPLYEKVGVKCPKCGGEIVKKKSKKGRTYYACENAPNCDFILWDKPVDEK
ECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCC
CPVCGSLLVEKNTKNGHILKCSNPECDYQKEVK
CCCCCHHEEECCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]