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The map label for this gene is rluB [H]

Identifier: 167040216

GI number: 167040216

Start: 1598567

End: 1599277

Strand: Direct

Name: rluB [H]

Synonym: Teth514_1578

Alternate gene names: 167040216

Gene position: 1598567-1599277 (Clockwise)

Preceding gene: 167040215

Following gene: 167040217

Centisome position: 65.05

GC content: 33.9

Gene sequence:

>711_bases
ATGGAAAGGTTGCAAAAATACTTAGCAGAATGTGGAATAGCCTCACGAAGAAAGTGCGAAGAGTATATTTTACAAGGCCG
TGTAAAAGTAAATGGAAAAGTAGTAAAAGAGTTGGGAGTAAAAATCAATCCTGATGTTGACATAATTGAATTTGATGATA
AAATTGTGAGAAGAGAAAAGAAAAAAGTTTATATTATGTTAAATAAGCCAACAGGATATATTACTTCCGTAAAAGACCAG
TTTGGCAGGCCTACAGTGATGGACTTAGTTAAAGTTAAGGAAAGAATATATCCTGTTGGCCGCTTAGATTATGATACATC
AGGGCTTCTGTTACTCACAAATGATGGGGAGATAGCCAATATCTTAATGCATCCTCGACATGAAATTGTGAAGACGTATA
TTGCAAAAATCAAAGGAATTCCAACACAAGAAGAGTTGGAAAGGTTTAGAAATGGACTTTTGATAGGAGATTATTTGACT
TCGAAAGCAGAAATAGAGATTTTAGCAGTTAAAAATGGGACAAGTGTTGTAGAAATAAAAATTCATGAAGGGAAAAATAG
ACAAGTTAGAAGAATGTGTGAGGCTATTGGACATCCTGTTATTTCTCTTACAAGAACTAAAATTGGTGACTTGTCTTTAG
AAGGGTTAAAACCAGGAAAGTGGAGGTATTTGACACAAAAGGAAATAGATTATTTAAAAAGCTTGAGGTGA

Upstream 100 bases:

>100_bases
ACTACTGATACCAACTTGATAAAAGAAAGGATCATGAGAAATAATTTATTAAAAATTTATGACGAAAAACTTGTATAAAC
GAAAAAATGGGTGAACACAA

Downstream 100 bases:

>100_bases
AGTATGTTTTCAGTTAACTTTGCTAAAGCAGAGGATTTCGAAATTATTAAAAAAATTGCTAATGAGTGTAATTTAAAGGG
AACTTTAAATTTTTTTGAAG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MERLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGVKINPDVDIIEFDDKIVRREKKKVYIMLNKPTGYITSVKDQ
FGRPTVMDLVKVKERIYPVGRLDYDTSGLLLLTNDGEIANILMHPRHEIVKTYIAKIKGIPTQEELERFRNGLLIGDYLT
SKAEIEILAVKNGTSVVEIKIHEGKNRQVRRMCEAIGHPVISLTRTKIGDLSLEGLKPGKWRYLTQKEIDYLKSLR

Sequences:

>Translated_236_residues
MERLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGVKINPDVDIIEFDDKIVRREKKKVYIMLNKPTGYITSVKDQ
FGRPTVMDLVKVKERIYPVGRLDYDTSGLLLLTNDGEIANILMHPRHEIVKTYIAKIKGIPTQEELERFRNGLLIGDYLT
SKAEIEILAVKNGTSVVEIKIHEGKNRQVRRMCEAIGHPVISLTRTKIGDLSLEGLKPGKWRYLTQKEIDYLKSLR
>Mature_236_residues
MERLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGVKINPDVDIIEFDDKIVRREKKKVYIMLNKPTGYITSVKDQ
FGRPTVMDLVKVKERIYPVGRLDYDTSGLLLLTNDGEIANILMHPRHEIVKTYIAKIKGIPTQEELERFRNGLLIGDYLT
SKAEIEILAVKNGTSVVEIKIHEGKNRQVRRMCEAIGHPVISLTRTKIGDLSLEGLKPGKWRYLTQKEIDYLKSLR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=35.8974358974359, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=38.0165289256198, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=34.051724137931, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=38.0681818181818, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27107; Mature: 27107

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGVKINPDVDIIEFDDKIVRREK
CHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCEEHHHCCCEECCCEEEEECCHHHHHCCC
KKVYIMLNKPTGYITSVKDQFGRPTVMDLVKVKERIYPVGRLDYDTSGLLLLTNDGEIAN
CEEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
ILMHPRHEIVKTYIAKIKGIPTQEELERFRNGLLIGDYLTSKAEIEILAVKNGTSVVEIK
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHCCCCEEEEEEEECCCEEEEEE
IHEGKNRQVRRMCEAIGHPVISLTRTKIGDLSLEGLKPGKWRYLTQKEIDYLKSLR
EECCCCHHHHHHHHHHCCCEEEEEHHHCCCEEECCCCCCCEEEECHHHHHHHHHCC
>Mature Secondary Structure
MERLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGVKINPDVDIIEFDDKIVRREK
CHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCEEHHHCCCEECCCEEEEECCHHHHHCCC
KKVYIMLNKPTGYITSVKDQFGRPTVMDLVKVKERIYPVGRLDYDTSGLLLLTNDGEIAN
CEEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
ILMHPRHEIVKTYIAKIKGIPTQEELERFRNGLLIGDYLTSKAEIEILAVKNGTSVVEIK
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHCCCCEEEEEEEECCCEEEEEE
IHEGKNRQVRRMCEAIGHPVISLTRTKIGDLSLEGLKPGKWRYLTQKEIDYLKSLR
EECCCCHHHHHHHHHHCCCEEEEEHHHCCCEEECCCCCCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]