Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 167040213

GI number: 167040213

Start: 1595063

End: 1596211

Strand: Direct

Name: dacB [H]

Synonym: Teth514_1575

Alternate gene names: 167040213

Gene position: 1595063-1596211 (Clockwise)

Preceding gene: 167040212

Following gene: 167040214

Centisome position: 64.91

GC content: 34.12

Gene sequence:

>1149_bases
ATGTATAAAATGAAAAAATTTGTGCTTTATCTTTTTATTTTGCTGTATTTTATGTCTTCTGTAAAAGTGTATGCTGAGAG
CTATCCTTCTATCTCTGCTAAAGCAGCAATTGTAATGGACCAAGAGACAGGGAGAGTGTTATATGAAAAAAATCCTCATG
AAAAGCTGCCAATGGCAAGCACCACAAAAATAATGACTTTATTGGTGGCTTTAGAAAAAGGTAATTTAAATGATATTGTA
ACTGTCAGCAAGAGGGCTGCAAGTGTTGGAGGTTCGTCTATTTGGCTTTCGCCAGGAGAAAAAATTGATATGGAAAGCTT
GCTTTATGGACTTATGCTAAACTCAGGGAACGATGCTGCCACAGCTATTGCAGAACATATAGGTGGTAGTGTAGAAAATT
TTATCGAAATTATGAACCAAAAAGCAAGGGAAATAGGGGCATATAATACACATTTTGTGACACCTTCTGGTTTAGATATT
GGTATTGATGACCATTATACTACCGCTTATGATTTAGCCTTAATAACGAGGTATGCTTTTAGATATCCTAAATTTGCAGA
GATTGTATCAACAAAAGAAAAGACAATTCCTTGGGAAGGGAAAGAATGGGATAGATATTTGAGGAATAAAAATAAGCTCT
TATGGATATACGAAGGAGCCGATGGGGTAAAAACTGGATTTACTAATAAAGCAGGTAGGTGTCTTGTATCTTCTGCTACA
AGAGATGGAAGGAGATTTATTGCAGTTGTTTTAAATAGTCCTCCTATGTGGGAAGATTCCATGAAAATATTAGACTATGC
TTTTTCAAAGTACAAACCCTATAAAGTTTTAGAAAAAGGGGAATTAATTAAGAAGATAAAAGTAGAAGGTGGAAAAGAAA
ATAGTGTATCGGTTGTAAGTGAGAGAGAATGTATCATTCCGGCATTAGAAGATGAGAAGCAGGATGTGAATTTAGAATTT
CATTTTCCCAAAAGTGTGAAAGCTCCTGTTGAAAAAGGTGAAAAAATAGGTTATGTGACAGTAAAAATAGGAGAAGAAAA
AATTTGTGATGTTAATTGTGTAGCCTTAAAGAAGGTGGAACAAGAAGATTTTGGTTACAATTTTCAAAAGATAATAAAAA
ATTGGTTAAAGGTGCTAAATCCCTCTTGA

Upstream 100 bases:

>100_bases
TTTTTTGGTGCCCAAATATACTAAACTAAAAATTGACAAAGTCAATATTGATGTGGAAAAGAAGCATCATAAGTGAGGTG
TAAAAAACTATAACTATAAC

Downstream 100 bases:

>100_bases
GTATAACTCAAGAGGGTTATTTGTTTTGTAATAGTTTTTAAGTTTAAAGAATATTTTATTAGTGTGAAATTGGAGGTGAG
ATAAATTGGCAAATAGATTA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MYKMKKFVLYLFILLYFMSSVKVYAESYPSISAKAAIVMDQETGRVLYEKNPHEKLPMASTTKIMTLLVALEKGNLNDIV
TVSKRAASVGGSSIWLSPGEKIDMESLLYGLMLNSGNDAATAIAEHIGGSVENFIEIMNQKAREIGAYNTHFVTPSGLDI
GIDDHYTTAYDLALITRYAFRYPKFAEIVSTKEKTIPWEGKEWDRYLRNKNKLLWIYEGADGVKTGFTNKAGRCLVSSAT
RDGRRFIAVVLNSPPMWEDSMKILDYAFSKYKPYKVLEKGELIKKIKVEGGKENSVSVVSERECIIPALEDEKQDVNLEF
HFPKSVKAPVEKGEKIGYVTVKIGEEKICDVNCVALKKVEQEDFGYNFQKIIKNWLKVLNPS

Sequences:

>Translated_382_residues
MYKMKKFVLYLFILLYFMSSVKVYAESYPSISAKAAIVMDQETGRVLYEKNPHEKLPMASTTKIMTLLVALEKGNLNDIV
TVSKRAASVGGSSIWLSPGEKIDMESLLYGLMLNSGNDAATAIAEHIGGSVENFIEIMNQKAREIGAYNTHFVTPSGLDI
GIDDHYTTAYDLALITRYAFRYPKFAEIVSTKEKTIPWEGKEWDRYLRNKNKLLWIYEGADGVKTGFTNKAGRCLVSSAT
RDGRRFIAVVLNSPPMWEDSMKILDYAFSKYKPYKVLEKGELIKKIKVEGGKENSVSVVSERECIIPALEDEKQDVNLEF
HFPKSVKAPVEKGEKIGYVTVKIGEEKICDVNCVALKKVEQEDFGYNFQKIIKNWLKVLNPS
>Mature_382_residues
MYKMKKFVLYLFILLYFMSSVKVYAESYPSISAKAAIVMDQETGRVLYEKNPHEKLPMASTTKIMTLLVALEKGNLNDIV
TVSKRAASVGGSSIWLSPGEKIDMESLLYGLMLNSGNDAATAIAEHIGGSVENFIEIMNQKAREIGAYNTHFVTPSGLDI
GIDDHYTTAYDLALITRYAFRYPKFAEIVSTKEKTIPWEGKEWDRYLRNKNKLLWIYEGADGVKTGFTNKAGRCLVSSAT
RDGRRFIAVVLNSPPMWEDSMKILDYAFSKYKPYKVLEKGELIKKIKVEGGKENSVSVVSERECIIPALEDEKQDVNLEF
HFPKSVKAPVEKGEKIGYVTVKIGEEKICDVNCVALKKVEQEDFGYNFQKIIKNWLKVLNPS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=31.7333333333333, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082022, Length=379, Percent_Identity=29.287598944591, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786851, Length=400, Percent_Identity=30.5, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87082054, Length=248, Percent_Identity=29.8387096774194, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43130; Mature: 43130

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKMKKFVLYLFILLYFMSSVKVYAESYPSISAKAAIVMDQETGRVLYEKNPHEKLPMAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCH
TTKIMTLLVALEKGNLNDIVTVSKRAASVGGSSIWLSPGEKIDMESLLYGLMLNSGNDAA
HHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHH
TAIAEHIGGSVENFIEIMNQKAREIGAYNTHFVTPSGLDIGIDDHYTTAYDLALITRYAF
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHH
RYPKFAEIVSTKEKTIPWEGKEWDRYLRNKNKLLWIYEGADGVKTGFTNKAGRCLVSSAT
CCCHHHHHHCCHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCC
RDGRRFIAVVLNSPPMWEDSMKILDYAFSKYKPYKVLEKGELIKKIKVEGGKENSVSVVS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
ERECIIPALEDEKQDVNLEFHFPKSVKAPVEKGEKIGYVTVKIGEEKICDVNCVALKKVE
CCCEECCCCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHH
QEDFGYNFQKIIKNWLKVLNPS
HHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKMKKFVLYLFILLYFMSSVKVYAESYPSISAKAAIVMDQETGRVLYEKNPHEKLPMAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCH
TTKIMTLLVALEKGNLNDIVTVSKRAASVGGSSIWLSPGEKIDMESLLYGLMLNSGNDAA
HHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHH
TAIAEHIGGSVENFIEIMNQKAREIGAYNTHFVTPSGLDIGIDDHYTTAYDLALITRYAF
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHH
RYPKFAEIVSTKEKTIPWEGKEWDRYLRNKNKLLWIYEGADGVKTGFTNKAGRCLVSSAT
CCCHHHHHHCCHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCC
RDGRRFIAVVLNSPPMWEDSMKILDYAFSKYKPYKVLEKGELIKKIKVEGGKENSVSVVS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
ERECIIPALEDEKQDVNLEFHFPKSVKAPVEKGEKIGYVTVKIGEEKICDVNCVALKKVE
CCCEECCCCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHH
QEDFGYNFQKIIKNWLKVLNPS
HHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]