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The map label for this gene is ppdK [H]

Identifier: 167039992

GI number: 167039992

Start: 1386624

End: 1389257

Strand: Direct

Name: ppdK [H]

Synonym: Teth514_1351

Alternate gene names: 167039992

Gene position: 1386624-1389257 (Clockwise)

Preceding gene: 167039991

Following gene: 167039993

Centisome position: 56.43

GC content: 38.04

Gene sequence:

>2634_bases
ATGAGTAAAAAGTATGTGTATTTATTTAGTGAAGGCGATGCTTCAATGAGAGAACTCTTAGGAGGAAAAGGCGCAAATCT
TGCTGAAATGACAAAGTTGGGTTTACCTGTTCCCCAAGGATTTACAGTCACCACTGAGGCTTGTACAAGGTATTATCAAG
ATGGTAAGACTATAGCTCCAGAAATTGTTGAACAAATCTATGAATATATGGCTAAATTAGAAGAAATTACAGGTAAAAAA
TTTGGAGACCCAACAAATCCTTTATTAGTATCTGTAAGGTCTGGCGCGAGAGTTTCAATGCCAGGTATGATGGATACTAT
TTTAAACCTTGGTTTAAACGACGAGACAGTAGAAGGCCTTGCTAAAGCTACAAACAATGAAAGATTTGCCTATGACAGCT
ATAGAAGATTTATTCAAATGTTCTCAGATGTTGTAATGGGAATTGACAAGAATAAATTTGAAGCTATTTTAGATGAAATA
AAAGAGGAAAATGGTGCAAAATTTGATACAGATTTAACAGCTGAAAACCTCAAAGAGGTAGTAAAAAGATTTAAAGAACT
TTATAAAAAAGAAATGGGAGTAGACTTCCCACAAGATCCAAAAGATCAACTTCTAGAAGCTGTAAAAGCAGTATTTAGGT
CTTGGGATAATCCAAGGGCTATAGTGTACAGAAGACTTAACGACATACCAAGTGATTGGGGTACAGCAGTAAATGTCCAA
TCAATGGTATTTGGCAACATGGGCAATGACTCAGGTACAGGTGTTGCTTTCACAAGAAATCCTGCAACTGGTGAAAAAGC
TTTATTTGGCGAATTCTTGATGAATGCTCAAGGTGAAGACGTTGTTGCAGGTATTAGAACGCCTCAGCCAATTTCAACTT
TGAAAGAGACTATGCCAGAAGTATATAACCAATTCGTAGAAATTGCTGAGAAATTGGAGAAACACTACAAAGACATGCAG
GACATAGAGTTTACTATAGAAAGAGGTAAACTCTACATGCTACAGACAAGAAACGGAAAGAGAACTGCTCAAGCAGCTTT
GAAAGTAGCTGTAGACTTAGTGGCTGAAGGCTTAATAGATGAAAAGACAGCAGTCTTAAGAGTAGATCCGAAACAATTGG
ACCAGTTATTGCATCCAACCTTTGAGCCAAATGCGTTGAAAGCTGCTAAGCCTGTTGCAAAAGGTTTACCTGCTTCTCCT
GGTGCTGCATCAGGAAAAGTATATTTTACTGCTGATGAGGCAATAGAAGCTGCAAAATCAAGAGGAGAAAAAGTAATTCT
TGTAAGAACAGAGACATCTCCAGATGACATTGAAGGTATGTCTGTAGCACAAGGAATACTTACAACTCGTGGCGGTATGA
CATCTCATGCAGCTGTTGTGGCAAGAGGTATGGGTACAGCTTGCGTAGTAGGTTGTGGCGATGCAAAGATAGACGAGCAA
GCAAAAGTAATGAGAATTGGCGATATAGAAGTTCATGAGGGAGACTACATATCTATAGATGGTAGCACAGGTAATGTGTA
CATTGGTGAAATAAAGACTGTTACACCTGAGCTAACTGGAGATTTTGCAACTCTCATGAGCTGGGCAGACAAATATAGAA
AATTAAAAGTAAAAACTAATGCAGATATACCAAGAGATGCAAAAGTTGCAGTTCAGTTTGGTGCTGAAGGAATAGGCCTT
TGCAGGACAGAGCATATGTTCTTTGATGAAGATAGAATTCCTGCTATGAGAGAAATGATTGTCTCAAAGACAGAAGAACA
AAGAAGAAAAGCTTTAGAAAAACTATTGCCTATGCAAAGGTCAGACTTTGAAGGTTTGTTTGAAGTTATGGGTGAATTCC
CTGTTACTATACGCCTTTTAGATCCACCACTGCATGAATTCTTACCTCATACAGATGAAGAAATTAAAGAACTTGCAGAA
AACATGGGTATAACTTTTGAAGAGCTAAAAGCAACAGTAGAAAGCTTAAAAGAGTTTAACCCAATGTTAGGTCATAGAGG
CTGTAGATTAGCTATAACCTATCCAGAAATTGCTGAAATGCAGACAAGAGCTATTATAGAAGCTGCTATAAACGTAAAGA
AGAAAACAGGCAAAGACGTAAAACCGCAAATTATGATACCACTTGTAGGTGAACTTAAAGAGCTTAAATACCTCAAAGAC
ATAATAGTAAAAGTAGCTGACGAAGTTATAAAAGAAAACAATGTTGAGATAAAATACCTTGTAGGAACAATGATAGAGGT
ACCAAGAGCAGCTCTTACAGCTGACCAAATTGCTAAGGAAGCAGAATTCTTCTCCTTTGGTACTAATGACTTGACTCAGA
TGACTTTCGGTTTCTCAAGGGATGATGCTGGCAAGTTCCTTGAAACCTATTATGAAAAGAAGATATATGAATTTGATCCA
TTTGCTAAGCTTGACCAAGAGGGTGTTGGCAAGTTAGTAGAAATGGGTACTAAACTTGGAAGACAGACAAGACCTGACCT
TGAAGTAGGTATATGTGGTGAGCACGGTGGAGACCCATCTTCTATTGAGTTCTGCCACAAAGTAGGTCTTGATTATGTCT
CTGCTTCACCTTACAGAGTACCTATTGCAAGACTGGCTGCTGCTCAGGCGGCAATAAAATACGATAATAAATAA

Upstream 100 bases:

>100_bases
TGATGAAGAGATTAGGGTGTACTGTAATCGATGTTACAAATAAAGCTGTAGAAGAAACAGCTAATGTGATATTAAACGTA
TTAAAAGGAGGAGAGATTTC

Downstream 100 bases:

>100_bases
TTAATTTCAAAGGCAGCAAATCAAAATTTGCTGCCTTTGAGCATTGTTTAAGGTTATTGTTTTTTGACCATTCATGTTAT
AATATTAAGTAAAATTGGTA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MSKKYVYLFSEGDASMRELLGGKGANLAEMTKLGLPVPQGFTVTTEACTRYYQDGKTIAPEIVEQIYEYMAKLEEITGKK
FGDPTNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLAKATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEI
KEENGAKFDTDLTAENLKEVVKRFKELYKKEMGVDFPQDPKDQLLEAVKAVFRSWDNPRAIVYRRLNDIPSDWGTAVNVQ
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQ
DIEFTIERGKLYMLQTRNGKRTAQAALKVAVDLVAEGLIDEKTAVLRVDPKQLDQLLHPTFEPNALKAAKPVAKGLPASP
GAASGKVYFTADEAIEAAKSRGEKVILVRTETSPDDIEGMSVAQGILTTRGGMTSHAAVVARGMGTACVVGCGDAKIDEQ
AKVMRIGDIEVHEGDYISIDGSTGNVYIGEIKTVTPELTGDFATLMSWADKYRKLKVKTNADIPRDAKVAVQFGAEGIGL
CRTEHMFFDEDRIPAMREMIVSKTEEQRRKALEKLLPMQRSDFEGLFEVMGEFPVTIRLLDPPLHEFLPHTDEEIKELAE
NMGITFEELKATVESLKEFNPMLGHRGCRLAITYPEIAEMQTRAIIEAAINVKKKTGKDVKPQIMIPLVGELKELKYLKD
IIVKVADEVIKENNVEIKYLVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDP
FAKLDQEGVGKLVEMGTKLGRQTRPDLEVGICGEHGGDPSSIEFCHKVGLDYVSASPYRVPIARLAAAQAAIKYDNK

Sequences:

>Translated_877_residues
MSKKYVYLFSEGDASMRELLGGKGANLAEMTKLGLPVPQGFTVTTEACTRYYQDGKTIAPEIVEQIYEYMAKLEEITGKK
FGDPTNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLAKATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEI
KEENGAKFDTDLTAENLKEVVKRFKELYKKEMGVDFPQDPKDQLLEAVKAVFRSWDNPRAIVYRRLNDIPSDWGTAVNVQ
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQ
DIEFTIERGKLYMLQTRNGKRTAQAALKVAVDLVAEGLIDEKTAVLRVDPKQLDQLLHPTFEPNALKAAKPVAKGLPASP
GAASGKVYFTADEAIEAAKSRGEKVILVRTETSPDDIEGMSVAQGILTTRGGMTSHAAVVARGMGTACVVGCGDAKIDEQ
AKVMRIGDIEVHEGDYISIDGSTGNVYIGEIKTVTPELTGDFATLMSWADKYRKLKVKTNADIPRDAKVAVQFGAEGIGL
CRTEHMFFDEDRIPAMREMIVSKTEEQRRKALEKLLPMQRSDFEGLFEVMGEFPVTIRLLDPPLHEFLPHTDEEIKELAE
NMGITFEELKATVESLKEFNPMLGHRGCRLAITYPEIAEMQTRAIIEAAINVKKKTGKDVKPQIMIPLVGELKELKYLKD
IIVKVADEVIKENNVEIKYLVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDP
FAKLDQEGVGKLVEMGTKLGRQTRPDLEVGICGEHGGDPSSIEFCHKVGLDYVSASPYRVPIARLAAAQAAIKYDNK
>Mature_876_residues
SKKYVYLFSEGDASMRELLGGKGANLAEMTKLGLPVPQGFTVTTEACTRYYQDGKTIAPEIVEQIYEYMAKLEEITGKKF
GDPTNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLAKATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIK
EENGAKFDTDLTAENLKEVVKRFKELYKKEMGVDFPQDPKDQLLEAVKAVFRSWDNPRAIVYRRLNDIPSDWGTAVNVQS
MVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQD
IEFTIERGKLYMLQTRNGKRTAQAALKVAVDLVAEGLIDEKTAVLRVDPKQLDQLLHPTFEPNALKAAKPVAKGLPASPG
AASGKVYFTADEAIEAAKSRGEKVILVRTETSPDDIEGMSVAQGILTTRGGMTSHAAVVARGMGTACVVGCGDAKIDEQA
KVMRIGDIEVHEGDYISIDGSTGNVYIGEIKTVTPELTGDFATLMSWADKYRKLKVKTNADIPRDAKVAVQFGAEGIGLC
RTEHMFFDEDRIPAMREMIVSKTEEQRRKALEKLLPMQRSDFEGLFEVMGEFPVTIRLLDPPLHEFLPHTDEEIKELAEN
MGITFEELKATVESLKEFNPMLGHRGCRLAITYPEIAEMQTRAIIEAAINVKKKTGKDVKPQIMIPLVGELKELKYLKDI
IVKVADEVIKENNVEIKYLVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDPF
AKLDQEGVGKLVEMGTKLGRQTRPDLEVGICGEHGGDPSSIEFCHKVGLDYVSASPYRVPIARLAAAQAAIKYDNK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=455, Percent_Identity=26.5934065934066, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1789193, Length=469, Percent_Identity=24.5202558635394, Blast_Score=104, Evalue=3e-23,
Organism=Escherichia coli, GI1787994, Length=703, Percent_Identity=24.1820768136558, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI48994992, Length=434, Percent_Identity=24.8847926267281, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788726, Length=459, Percent_Identity=23.5294117647059, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97406; Mature: 97275

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYVYLFSEGDASMRELLGGKGANLAEMTKLGLPVPQGFTVTTEACTRYYQDGKTIAP
CCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCHH
EIVEQIYEYMAKLEEITGKKFGDPTNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHH
AKATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIKEENGAKFDTDLTAENLKEV
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VKRFKELYKKEMGVDFPQDPKDQLLEAVKAVFRSWDNPRAIVYRRLNDIPSDWGTAVNVQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEH
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPE
HHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
VYNQFVEIAEKLEKHYKDMQDIEFTIERGKLYMLQTRNGKRTAQAALKVAVDLVAEGLID
HHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
EKTAVLRVDPKQLDQLLHPTFEPNALKAAKPVAKGLPASPGAASGKVYFTADEAIEAAKS
CCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHC
RGEKVILVRTETSPDDIEGMSVAQGILTTRGGMTSHAAVVARGMGTACVVGCGDAKIDEQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCC
AKVMRIGDIEVHEGDYISIDGSTGNVYIGEIKTVTPELTGDFATLMSWADKYRKLKVKTN
CCEEEECCEEEECCCEEEEECCCCCEEEEEEEEECCHHCCHHHHHHHHHHHHHEEEEECC
ADIPRDAKVAVQFGAEGIGLCRTEHMFFDEDRIPAMREMIVSKTEEQRRKALEKLLPMQR
CCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCC
SDFEGLFEVMGEFPVTIRLLDPPLHEFLPHTDEEIKELAENMGITFEELKATVESLKEFN
CCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PMLGHRGCRLAITYPEIAEMQTRAIIEAAINVKKKTGKDVKPQIMIPLVGELKELKYLKD
HHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHH
IIVKVADEVIKENNVEIKYLVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
DDAGKFLETYYEKKIYEFDPFAKLDQEGVGKLVEMGTKLGRQTRPDLEVGICGEHGGDPS
CHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCH
SIEFCHKVGLDYVSASPYRVPIARLAAAQAAIKYDNK
HHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKKYVYLFSEGDASMRELLGGKGANLAEMTKLGLPVPQGFTVTTEACTRYYQDGKTIAP
CCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCHH
EIVEQIYEYMAKLEEITGKKFGDPTNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHH
AKATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIKEENGAKFDTDLTAENLKEV
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VKRFKELYKKEMGVDFPQDPKDQLLEAVKAVFRSWDNPRAIVYRRLNDIPSDWGTAVNVQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEH
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPE
HHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
VYNQFVEIAEKLEKHYKDMQDIEFTIERGKLYMLQTRNGKRTAQAALKVAVDLVAEGLID
HHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
EKTAVLRVDPKQLDQLLHPTFEPNALKAAKPVAKGLPASPGAASGKVYFTADEAIEAAKS
CCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHC
RGEKVILVRTETSPDDIEGMSVAQGILTTRGGMTSHAAVVARGMGTACVVGCGDAKIDEQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCC
AKVMRIGDIEVHEGDYISIDGSTGNVYIGEIKTVTPELTGDFATLMSWADKYRKLKVKTN
CCEEEECCEEEECCCEEEEECCCCCEEEEEEEEECCHHCCHHHHHHHHHHHHHEEEEECC
ADIPRDAKVAVQFGAEGIGLCRTEHMFFDEDRIPAMREMIVSKTEEQRRKALEKLLPMQR
CCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCC
SDFEGLFEVMGEFPVTIRLLDPPLHEFLPHTDEEIKELAENMGITFEELKATVESLKEFN
CCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PMLGHRGCRLAITYPEIAEMQTRAIIEAAINVKKKTGKDVKPQIMIPLVGELKELKYLKD
HHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHH
IIVKVADEVIKENNVEIKYLVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
DDAGKFLETYYEKKIYEFDPFAKLDQEGVGKLVEMGTKLGRQTRPDLEVGICGEHGGDPS
CHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCH
SIEFCHKVGLDYVSASPYRVPIARLAAAQAAIKYDNK
HHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]