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The map label for this gene is thrS [H]

Identifier: 167039934

GI number: 167039934

Start: 1326926

End: 1328569

Strand: Direct

Name: thrS [H]

Synonym: Teth514_1290

Alternate gene names: 167039934

Gene position: 1326926-1328569 (Clockwise)

Preceding gene: 167039932

Following gene: 167039935

Centisome position: 54.0

GC content: 33.7

Gene sequence:

>1644_bases
ATGAAAATTGCTATTGCGGGTGATATTTATGAATACCCAGAAGGGACAAAATTAGAGGATATTGCCAAAGATTTTGAGCA
TCTTTACAATGGGATAATAGTAGCTGCAAAAGTGGATAATGAGCTAAAAGATTTAAACTGCACAGTTTCAAAGGATAGCA
CAGTGGAATTTGTAGATACAACGATTGAGGAAGGTACGAGGATTTACAGAAGGAGTTTAACTTTTGTTTTTATTAAAGCT
GTAAAAGAGCTACTTCCCGGTGCTACTGTAACTATAGAACACTCACTAGGTAAGGGTTTATACTGCGAAATACATGGTTA
CTCTTTAAACAATAAAATTGTCTCTATGATTAAAAAGCGGATGGAAGAAATAATAAAAAAAGACTTCAAAATAGAAAGAA
AAATGTTACCTAAAGAAGAGGCAATAAAATTATTTAAAAAAGAAGGATTGACTGAGAAAATAAAATTGTTTAAAGATACT
GACAAAGAGAAGGTTTCTGTTTATTATTGTGATGGTACAGTAGATTTTTTCTACAGCCCTTGTGTACCTTCTACAGGGTA
TCTAAAAGTATTTGATATACGTTTTTATTTTCCAGGCATTATTTTAATTGCTCCAGATGTATATAATCCCCGCTCTTTGC
CTGTATTTGTGGATGTTCCAAAACTTGCTTCAATTTTTAAAGAGGCAGAGGACTGGGCAAGTATTTTAAACATAAGTTAT
GTTTCTTCTTTGAACGACATGATAAAACAAGGAAGAGGTAGAGATTTAATATTGGTTTCTGAAGCATTTCATGAGAAGAA
AATATCCAAAATTGCAGATTATATAGCTTCAAACAAAATGATAAAAGTAGTGCTTATTGCTGGACCCTCTTCTTCAGGGA
AAACCTCTTTTATTCATAGACTTAGTGTGCAGTTGAGGGTAAACGGACTAAAGCCTTTTCCGATATCATTAGACAATTAT
TTTGTGCCAAGAGAACTTACTCCGAAAGATGAGTTTGGAAATTACGACTTTGAGTCCATAGATGCATTAGATTTGCCACT
TTTCAATGAGCATTTAATAAAGCTTATTCAAGGGGAAGAAGTAGAAATCCCTATTTTTAACTTTAAAACAGGGGAAAGAG
AGCCAGAAGGAAGAAAAGTAAAACTTGACAAAAATCAAATTATTTTGATGGAAGGGATTCACGGTCTAAATGAAAAATTG
ACATTGCAAATACCCAAAGATAATAAATACAAAATATATGTAAGTGCTATTACGCAATTAAATCTTGACGAGCACAACAG
AATACCTACTACTCAAACGAGGTTGATACGACGCATAGTGAGAGATAGCCAATTCCGGTCCAGCGATGCTGCAGAGACTA
TAAACATGTGGCCTATGGTCAGAAAAGGAGAAGAAAAATGGATTTTCCCTTATCAAGAACAAGCGGATGTCATGTTTAAC
TCTTTTTTACCTTATGAGCTACCTGTATTAAAAAAATATGCAGAGCCTTTGCTTAAGAAAGTTTCCCAAGACAATCCTGC
TTATTCTATCGCTAAAGAAATATTAGAGTTTTTGAGTTATTTCCTTCCATTAGAAGATGAATTAGCAATACCTCCCAATT
CTATCATAAGAGAATTTATAGGCGGCAGTTGCCTTGATGTTTAA

Upstream 100 bases:

>100_bases
ATAGCCTTACGGCTTCGTGGGACAAAAATTAAAAGTCCTTCCGGAAGGAAGGGCTTTTAATTTTTGTTACAATAATGTAT
AATCCTAGTGAGGTGGTTTT

Downstream 100 bases:

>100_bases
GTTTAGGAATCCTCAATGGATTCCTACTCGATTTTGTAGAATTTTTGCAAAAAAAGAAGGAATATTTAAAGTTTTGTAGA
AATAAAGAGAAAAGGCTTTA

Product: phosphoribulokinase/uridine kinase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MKIAIAGDIYEYPEGTKLEDIAKDFEHLYNGIIVAAKVDNELKDLNCTVSKDSTVEFVDTTIEEGTRIYRRSLTFVFIKA
VKELLPGATVTIEHSLGKGLYCEIHGYSLNNKIVSMIKKRMEEIIKKDFKIERKMLPKEEAIKLFKKEGLTEKIKLFKDT
DKEKVSVYYCDGTVDFFYSPCVPSTGYLKVFDIRFYFPGIILIAPDVYNPRSLPVFVDVPKLASIFKEAEDWASILNISY
VSSLNDMIKQGRGRDLILVSEAFHEKKISKIADYIASNKMIKVVLIAGPSSSGKTSFIHRLSVQLRVNGLKPFPISLDNY
FVPRELTPKDEFGNYDFESIDALDLPLFNEHLIKLIQGEEVEIPIFNFKTGEREPEGRKVKLDKNQIILMEGIHGLNEKL
TLQIPKDNKYKIYVSAITQLNLDEHNRIPTTQTRLIRRIVRDSQFRSSDAAETINMWPMVRKGEEKWIFPYQEQADVMFN
SFLPYELPVLKKYAEPLLKKVSQDNPAYSIAKEILEFLSYFLPLEDELAIPPNSIIREFIGGSCLDV

Sequences:

>Translated_547_residues
MKIAIAGDIYEYPEGTKLEDIAKDFEHLYNGIIVAAKVDNELKDLNCTVSKDSTVEFVDTTIEEGTRIYRRSLTFVFIKA
VKELLPGATVTIEHSLGKGLYCEIHGYSLNNKIVSMIKKRMEEIIKKDFKIERKMLPKEEAIKLFKKEGLTEKIKLFKDT
DKEKVSVYYCDGTVDFFYSPCVPSTGYLKVFDIRFYFPGIILIAPDVYNPRSLPVFVDVPKLASIFKEAEDWASILNISY
VSSLNDMIKQGRGRDLILVSEAFHEKKISKIADYIASNKMIKVVLIAGPSSSGKTSFIHRLSVQLRVNGLKPFPISLDNY
FVPRELTPKDEFGNYDFESIDALDLPLFNEHLIKLIQGEEVEIPIFNFKTGEREPEGRKVKLDKNQIILMEGIHGLNEKL
TLQIPKDNKYKIYVSAITQLNLDEHNRIPTTQTRLIRRIVRDSQFRSSDAAETINMWPMVRKGEEKWIFPYQEQADVMFN
SFLPYELPVLKKYAEPLLKKVSQDNPAYSIAKEILEFLSYFLPLEDELAIPPNSIIREFIGGSCLDV
>Mature_547_residues
MKIAIAGDIYEYPEGTKLEDIAKDFEHLYNGIIVAAKVDNELKDLNCTVSKDSTVEFVDTTIEEGTRIYRRSLTFVFIKA
VKELLPGATVTIEHSLGKGLYCEIHGYSLNNKIVSMIKKRMEEIIKKDFKIERKMLPKEEAIKLFKKEGLTEKIKLFKDT
DKEKVSVYYCDGTVDFFYSPCVPSTGYLKVFDIRFYFPGIILIAPDVYNPRSLPVFVDVPKLASIFKEAEDWASILNISY
VSSLNDMIKQGRGRDLILVSEAFHEKKISKIADYIASNKMIKVVLIAGPSSSGKTSFIHRLSVQLRVNGLKPFPISLDNY
FVPRELTPKDEFGNYDFESIDALDLPLFNEHLIKLIQGEEVEIPIFNFKTGEREPEGRKVKLDKNQIILMEGIHGLNEKL
TLQIPKDNKYKIYVSAITQLNLDEHNRIPTTQTRLIRRIVRDSQFRSSDAAETINMWPMVRKGEEKWIFPYQEQADVMFN
SFLPYELPVLKKYAEPLLKKVSQDNPAYSIAKEILEFLSYFLPLEDELAIPPNSIIREFIGGSCLDV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0572

COG function: function code F; Uridine kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788013, Length=190, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 62740; Mature: 62740

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAGDIYEYPEGTKLEDIAKDFEHLYNGIIVAAKVDNELKDLNCTVSKDSTVEFVDT
CEEEEECCHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHCCEEEECCCCCCHHHHH
TIEEGTRIYRRSLTFVFIKAVKELLPGATVTIEHSLGKGLYCEIHGYSLNNKIVSMIKKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHH
MEEIIKKDFKIERKMLPKEEAIKLFKKEGLTEKIKLFKDTDKEKVSVYYCDGTVDFFYSP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCEEEEEECC
CVPSTGYLKVFDIRFYFPGIILIAPDVYNPRSLPVFVDVPKLASIFKEAEDWASILNISY
CCCCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
VSSLNDMIKQGRGRDLILVSEAFHEKKISKIADYIASNKMIKVVLIAGPSSSGKTSFIHR
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHEEEE
LSVQLRVNGLKPFPISLDNYFVPRELTPKDEFGNYDFESIDALDLPLFNEHLIKLIQGEE
EEEEEEECCCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCEECCCHHHHHHHHHHCCCC
VEIPIFNFKTGEREPEGRKVKLDKNQIILMEGIHGLNEKLTLQIPKDNKYKIYVSAITQL
EEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEC
NLDEHNRIPTTQTRLIRRIVRDSQFRSSDAAETINMWPMVRKGEEKWIFPYQEQADVMFN
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEECCCCCEECCCCHHHHHHHH
SFLPYELPVLKKYAEPLLKKVSQDNPAYSIAKEILEFLSYFLPLEDELAIPPNSIIREFI
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GGSCLDV
CCCCCCC
>Mature Secondary Structure
MKIAIAGDIYEYPEGTKLEDIAKDFEHLYNGIIVAAKVDNELKDLNCTVSKDSTVEFVDT
CEEEEECCHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHCCEEEECCCCCCHHHHH
TIEEGTRIYRRSLTFVFIKAVKELLPGATVTIEHSLGKGLYCEIHGYSLNNKIVSMIKKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHH
MEEIIKKDFKIERKMLPKEEAIKLFKKEGLTEKIKLFKDTDKEKVSVYYCDGTVDFFYSP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCEEEEEECC
CVPSTGYLKVFDIRFYFPGIILIAPDVYNPRSLPVFVDVPKLASIFKEAEDWASILNISY
CCCCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
VSSLNDMIKQGRGRDLILVSEAFHEKKISKIADYIASNKMIKVVLIAGPSSSGKTSFIHR
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHEEEE
LSVQLRVNGLKPFPISLDNYFVPRELTPKDEFGNYDFESIDALDLPLFNEHLIKLIQGEE
EEEEEEECCCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCEECCCHHHHHHHHHHCCCC
VEIPIFNFKTGEREPEGRKVKLDKNQIILMEGIHGLNEKLTLQIPKDNKYKIYVSAITQL
EEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEC
NLDEHNRIPTTQTRLIRRIVRDSQFRSSDAAETINMWPMVRKGEEKWIFPYQEQADVMFN
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEECCCCCEECCCCHHHHHHHH
SFLPYELPVLKKYAEPLLKKVSQDNPAYSIAKEILEFLSYFLPLEDELAIPPNSIIREFI
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GGSCLDV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA