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The map label for this gene is hsdS [H]

Identifier: 167039866

GI number: 167039866

Start: 1263177

End: 1264568

Strand: Reverse

Name: hsdS [H]

Synonym: Teth514_1221

Alternate gene names: 167039866

Gene position: 1264568-1263177 (Counterclockwise)

Preceding gene: 167039867

Following gene: 167039865

Centisome position: 51.46

GC content: 36.71

Gene sequence:

>1392_bases
ATGCTGAAGCCCTATCCAAAATATAAAGAAACTCCTGCTCTATGGCTTAATAGTATCCCAAACCATTGGGAATCTCATAA
AATACGTGAACTTTTTGTGGAAAGAAGTGAAAAAGTATCAGATAAGGATTATTCACCTCTTTCTGTAAGTAAAGCTGGGG
TTGTCCCTCAAATTGCTACTGTTGCAAAAACAAATAATGGTGATAATCGTAAACTTGTTATAAAAGGAGATTTTGTAATA
AATAGTCGTTCGGATAGAAGAGGTTCCAGCGGCATATCCAATTATGATGGTTCTGTTTCACTAATTAACATAGTGTTAAA
GCCGCGCTCTTTTGTTAATGGTCGATATATGCATTATCTGCTTAAAAGCCATTATTTTATCGAAGAGTTTTACAGAAATG
GTCGAGGAATTGTTGCTGACCTGTGGACAACTAGATATACAGAAATGAAATCTATATATCTGCCAGTTCCAAGCATTGAG
GAACAAGACCAGATTGTGCGTTTTCTTGACTGGAAGCTTGCTAAAATCAATAAACTTATCCAGGCAAAGAAAAAGCAGAT
CGCACTGCTGACCGAGTACCGCAAGGCTACCATTGATAACGTAATTATGTATGGAATTAATCCACACGCAAATAGAAAAG
AAAGTGGAGTAATCTGGTTAGGTGAAATTCCGTCGCATTGGTCTGTTATGAAGCTTAAGCGTATTTGCCGAATTAATGCA
TCCATTACATCTCAACTAGAGAAATACTCTTTAGAAGATTATGTAGTCTTTCTACCAATGGAGAATATTTCATCGGATGG
AAAAATTGATTGTTGCGAAAAACGCAAACTCAAAGATGTAAGAAATGGTTTTTCATCTTTTGCTAAAAATGACGTTATTG
TTGCAAAGATTACCCCATGTTTTGAAAATGGTAAGGGTGCTTGCTTAGATACCCTTGAAACAAATATTGGTTTTGGTACT
ACAGAATTAATTGTATTAAGAGCAAACGAAAAAGTTCTGCCACGCTATCTATATATGATTACACAATTACAACAATTTCG
TATAGAAGGCGCTAATGTAATGACAGGTTCTGCCGGGCAAAAACGGGTTCCCAGCAGCTTCATAAGTAACTTCGAGTTGG
GAATTCCTAGTATAGCCGAGCAAAGTGAAATTCTTGAATATCTCGATAATCGTTTAGCTAAGTTCGATAAGTTGTACGAA
ACATTAAATCGTGAAATAGAACTATTAACAGAATACCGTATCCGCCTTATTTCGGATGTGGTAACAGGGAAAGTCGATGT
ACGTGATATCGAAATACCTGAATATGAAGGCGACTCTGATGAAACTATCGACGGTGAAATTGATGATAATATCGTCCTTG
AGGAAGAAGGCGGAGAAATGGAGGTGGAATAA

Upstream 100 bases:

>100_bases
CCAATTCAGCTGCGAACACTAGAAGAAATAACAGCAGACATTCGTGCTCTCGAAGTAGAGACAGACGGATTACTTGCTGA
GATTATAGGGGGTTGAGAAT

Downstream 100 bases:

>100_bases
CATGACCGACGATATTCATATGGAAATATCTATACCAGCAGATAACGATGGATATATACTTTTGCAATGCCCCAACTGTG
GTACTTTCTTCAAAGCTACT

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.StySJI; Type I restriction enzyme StySJI specificity protein; S protein [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLKPYPKYKETPALWLNSIPNHWESHKIRELFVERSEKVSDKDYSPLSVSKAGVVPQIATVAKTNNGDNRKLVIKGDFVI
NSRSDRRGSSGISNYDGSVSLINIVLKPRSFVNGRYMHYLLKSHYFIEEFYRNGRGIVADLWTTRYTEMKSIYLPVPSIE
EQDQIVRFLDWKLAKINKLIQAKKKQIALLTEYRKATIDNVIMYGINPHANRKESGVIWLGEIPSHWSVMKLKRICRINA
SITSQLEKYSLEDYVVFLPMENISSDGKIDCCEKRKLKDVRNGFSSFAKNDVIVAKITPCFENGKGACLDTLETNIGFGT
TELIVLRANEKVLPRYLYMITQLQQFRIEGANVMTGSAGQKRVPSSFISNFELGIPSIAEQSEILEYLDNRLAKFDKLYE
TLNREIELLTEYRIRLISDVVTGKVDVRDIEIPEYEGDSDETIDGEIDDNIVLEEEGGEMEVE

Sequences:

>Translated_463_residues
MLKPYPKYKETPALWLNSIPNHWESHKIRELFVERSEKVSDKDYSPLSVSKAGVVPQIATVAKTNNGDNRKLVIKGDFVI
NSRSDRRGSSGISNYDGSVSLINIVLKPRSFVNGRYMHYLLKSHYFIEEFYRNGRGIVADLWTTRYTEMKSIYLPVPSIE
EQDQIVRFLDWKLAKINKLIQAKKKQIALLTEYRKATIDNVIMYGINPHANRKESGVIWLGEIPSHWSVMKLKRICRINA
SITSQLEKYSLEDYVVFLPMENISSDGKIDCCEKRKLKDVRNGFSSFAKNDVIVAKITPCFENGKGACLDTLETNIGFGT
TELIVLRANEKVLPRYLYMITQLQQFRIEGANVMTGSAGQKRVPSSFISNFELGIPSIAEQSEILEYLDNRLAKFDKLYE
TLNREIELLTEYRIRLISDVVTGKVDVRDIEIPEYEGDSDETIDGEIDDNIVLEEEGGEMEVE
>Mature_463_residues
MLKPYPKYKETPALWLNSIPNHWESHKIRELFVERSEKVSDKDYSPLSVSKAGVVPQIATVAKTNNGDNRKLVIKGDFVI
NSRSDRRGSSGISNYDGSVSLINIVLKPRSFVNGRYMHYLLKSHYFIEEFYRNGRGIVADLWTTRYTEMKSIYLPVPSIE
EQDQIVRFLDWKLAKINKLIQAKKKQIALLTEYRKATIDNVIMYGINPHANRKESGVIWLGEIPSHWSVMKLKRICRINA
SITSQLEKYSLEDYVVFLPMENISSDGKIDCCEKRKLKDVRNGFSSFAKNDVIVAKITPCFENGKGACLDTLETNIGFGT
TELIVLRANEKVLPRYLYMITQLQQFRIEGANVMTGSAGQKRVPSSFISNFELGIPSIAEQSEILEYLDNRLAKFDKLYE
TLNREIELLTEYRIRLISDVVTGKVDVRDIEIPEYEGDSDETIDGEIDDNIVLEEEGGEMEVE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 52996; Mature: 52996

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPYPKYKETPALWLNSIPNHWESHKIRELFVERSEKVSDKDYSPLSVSKAGVVPQIAT
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VAKTNNGDNRKLVIKGDFVINSRSDRRGSSGISNYDGSVSLINIVLKPRSFVNGRYMHYL
HEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCHHHCHHHHHHH
LKSHYFIEEFYRNGRGIVADLWTTRYTEMKSIYLPVPSIEEQDQIVRFLDWKLAKINKLI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QAKKKQIALLTEYRKATIDNVIMYGINPHANRKESGVIWLGEIPSHWSVMKLKRICRINA
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCH
SITSQLEKYSLEDYVVFLPMENISSDGKIDCCEKRKLKDVRNGFSSFAKNDVIVAKITPC
HHHHHHHHCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCH
FENGKGACLDTLETNIGFGTTELIVLRANEKVLPRYLYMITQLQQFRIEGANVMTGSAGQ
HHCCCCCEEHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KRVPSSFISNFELGIPSIAEQSEILEYLDNRLAKFDKLYETLNREIELLTEYRIRLISDV
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTGKVDVRDIEIPEYEGDSDETIDGEIDDNIVLEEEGGEMEVE
HCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEECC
>Mature Secondary Structure
MLKPYPKYKETPALWLNSIPNHWESHKIRELFVERSEKVSDKDYSPLSVSKAGVVPQIAT
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VAKTNNGDNRKLVIKGDFVINSRSDRRGSSGISNYDGSVSLINIVLKPRSFVNGRYMHYL
HEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCHHHCHHHHHHH
LKSHYFIEEFYRNGRGIVADLWTTRYTEMKSIYLPVPSIEEQDQIVRFLDWKLAKINKLI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QAKKKQIALLTEYRKATIDNVIMYGINPHANRKESGVIWLGEIPSHWSVMKLKRICRINA
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCH
SITSQLEKYSLEDYVVFLPMENISSDGKIDCCEKRKLKDVRNGFSSFAKNDVIVAKITPC
HHHHHHHHCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCH
FENGKGACLDTLETNIGFGTTELIVLRANEKVLPRYLYMITQLQQFRIEGANVMTGSAGQ
HHCCCCCEEHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KRVPSSFISNFELGIPSIAEQSEILEYLDNRLAKFDKLYETLNREIELLTEYRIRLISDV
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTGKVDVRDIEIPEYEGDSDETIDGEIDDNIVLEEEGGEMEVE
HCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2838725; 11677609; 3025838 [H]