| Definition | Thermoanaerobacter sp. X514 chromosome, complete genome. |
|---|---|
| Accession | NC_010320 |
| Length | 2,457,259 |
Click here to switch to the map view.
The map label for this gene is metH [H]
Identifier: 167039856
GI number: 167039856
Start: 1252326
End: 1254749
Strand: Direct
Name: metH [H]
Synonym: Teth514_1211
Alternate gene names: 167039856
Gene position: 1252326-1254749 (Clockwise)
Preceding gene: 167039855
Following gene: 167039857
Centisome position: 50.96
GC content: 35.89
Gene sequence:
>2424_bases ATGTTAGACATTTTTAAAGAACTTTCAAATAGAGTGATTGTTTTTGATGGAGCAATGGGGACACAACTTCAAGAGAGGGG TTTAAAAACTGGAGAGTGTCCCGAATATATGAATATAACCCATCCAGAAGTAGTATTTGATATCCACAGGTCCTATATAG AAGCAGGAGCGGATGTGATTGAGACAAATACCTTTGGCGCTAACAGGATAAAATTAGCAAAATATGGACTGGAAAATGAG GTTTTCAATATTGTCACTCAGGCTGTCAAAATTGCCAAAGAAGCTTCCAAAGATAAACCTGTGGCACTTTCTATAGGACC TATTGGAGAACTTTTGACCCCTTATGGAGACATGACTTTTGACGAAGCTTATGATGTTTTTAAAGAAGTAGTTATAGCTG CAGAAAGAGCGGGAGCAGATATTGTTTTAATTGAAACAATGTCAGATATGCTGGAGGCAAAAGCTGCCATTCTTGCTGCT AAAGAGAATTCAAACATGAAAGTTATATGTACAATGACTTTTCAAGAAGATGGAAGGACTCTCATGGGGTCAGACCCTAT AACTGTAGTAGTTAGTTTACAAGGGTTAGGATTAGATGCCATTGGCGTTAACTGCTCGACAGGTCCCGATAAAATGGTAA GTGTAGTGGAAAAAATGTCTCAAGTTTCTCGCATACCTATTATTGCACAACCTAATGCAGGTATGCCTGTCATTCGCGAC GGCAAAACAGTTTACGACTTAAAACCAGAAGAATTTGCTAGTTTCTTTCCTTCTCTTGTTGAAAAAGGAGCATCTATAGT GGGAGGGTGCTGCGGTACTACTCCTCACTATATAAAACTTGTTAAAAAAGCTGTAAAAGATTTAAAGCCAAAAGTCAAAG TCAACAAATTTACAGCTGTAGCTTCCAATACAAAAACAGTTTTCATAGGCCACGATTATTCTTTAAGGGTTATAGGGGAA CGTATAAATCCTACTGGAAAAAAGAAGTTAAGTGAGGCTTTTTTGGCGGGAAATGTGAGTTTAGCAGTAGAGGAAGCTAT AAAACAGCAAAAATGCGGAGCGGAAATATTGGATGTAAATGTGGGAGTTCCTGGGGTAAACGAAGAAGAATTACTTCCTA AAGTAGTTTCTGAAATACAAAATGTGGTAGACATTCCTCTTCAAATAGATAGCACTAATATTAAAGCTGTAGAAAAAGCT ATAAGAATTTTAAGAGGTAGACCCATAATCAACTCTGTAAGTGCAAAAGAGGAAAGTTTAAAAGAAGTGCTTCCTATTGT CAAAAAATACGGAGCTTGTGTTGTAGGACTTACTGTAGGAGATAAAGGGCTTCCCAAAGATAGGCATGAAAGAATTGAAA ATGCGAAAAAAATTATAAAAAAAGCGGAAGAGTATGGCATACCTAAAGAGGACATATTGATAGATTGTATTGTGCTGACG GTTTCTTCTGAGCAGGAGGCCGCAATTGAGACTTTAGAAGCGATAAAATTGGCTAAAGAAGAGTTAGGAGCAAATACTGT TGTGGGACTTAGCAATGTTTCCTTTGGTTTACCTGAAAGAAGGTTGATAAACTCTACTTTTTTAGCAATGGCAGCATCTT ATGGGCTTACTACTGCTATTATAAATCCCTGCGATGAAGCCATGATGGACACTTTGAGGGCATCAATGGTTTTACTAAAT AAAGATAAAGGCAGTGTAAATTATCTAAAAATATATGGTAACAGGCAAAAAGAGGAAGGTAAAGAAAAAGAACAGCAAAA AATTCAGGAAGAAGATTTAAAGTCAAAGTTCTATATCCAAATTTTAGAAGGTAAAAAATCAGGAGTAGAAGATATAGTAA AAAATATTTTAGAGAAGGAAGTTCAGCCTCTTTCTATAGTTGATAACATAATAATTCCTGCCTTAAAAGAAGTGGGAGAT AGATATGAAAAAGGCATATATTTCTTGCCACAACTTTTGAGCTCTGCAGAAGTGGTGCAAAATGCTTTTAGGATAATAAA GGAAAAGCTTCCAAAAGGTTCAGTATCTAAAGGCAAAATAATACTTGCTACAGTTGAAGGAGATGTACATGATATAGGAA AAAATATAGTAAAAGTTTTGCTTGAAAACTATGGATATGATGTAATTGACTTAGGAAAAGATGTCAAAGGAGAAGTGATA TTAGAGGAAGTAAAGCGAACAGGGGCACCGTTAGTAGGTCTAAGTGCTTTGATGACGACAACTTTGTTTAATATGGAGAA AATTATCAAACTGCTTAAAGCAAATACTGATGTGAAAATAATGGTAGGCGGGGCTGTTTTGACTGAGGAATATGCCTATA AGATTGGAGCAGATTATTACGGAAAAACGGCTCAAGATGCAGTTAAAATTGCTGATAAGTTTTTCTTAAAAAATGCTCTT TTATGTAAAACTTGTGGTATTTAA
Upstream 100 bases:
>100_bases GGGAGTACAAATGTTGTGTTAGAAATAATTTCAAGAGTCGATGATTTAGTCTGTGATTTTGCAGAAAATAACGTACAGAT GAATTAAATGGAGGTTAAAA
Downstream 100 bases:
>100_bases AGGTGGTGTAGAGGTGTCAGTAGAAGCGGTGAAAGAGTTTTTTAAAGAGAAAGGATATGATATTAAAATAAAAATTTTTG AAGATACAAGCACTGTGGAA
Product: homocysteine S-methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 807; Mature: 807
Protein sequence:
>807_residues MLDIFKELSNRVIVFDGAMGTQLQERGLKTGECPEYMNITHPEVVFDIHRSYIEAGADVIETNTFGANRIKLAKYGLENE VFNIVTQAVKIAKEASKDKPVALSIGPIGELLTPYGDMTFDEAYDVFKEVVIAAERAGADIVLIETMSDMLEAKAAILAA KENSNMKVICTMTFQEDGRTLMGSDPITVVVSLQGLGLDAIGVNCSTGPDKMVSVVEKMSQVSRIPIIAQPNAGMPVIRD GKTVYDLKPEEFASFFPSLVEKGASIVGGCCGTTPHYIKLVKKAVKDLKPKVKVNKFTAVASNTKTVFIGHDYSLRVIGE RINPTGKKKLSEAFLAGNVSLAVEEAIKQQKCGAEILDVNVGVPGVNEEELLPKVVSEIQNVVDIPLQIDSTNIKAVEKA IRILRGRPIINSVSAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERIENAKKIIKKAEEYGIPKEDILIDCIVLT VSSEQEAAIETLEAIKLAKEELGANTVVGLSNVSFGLPERRLINSTFLAMAASYGLTTAIINPCDEAMMDTLRASMVLLN KDKGSVNYLKIYGNRQKEEGKEKEQQKIQEEDLKSKFYIQILEGKKSGVEDIVKNILEKEVQPLSIVDNIIIPALKEVGD RYEKGIYFLPQLLSSAEVVQNAFRIIKEKLPKGSVSKGKIILATVEGDVHDIGKNIVKVLLENYGYDVIDLGKDVKGEVI LEEVKRTGAPLVGLSALMTTTLFNMEKIIKLLKANTDVKIMVGGAVLTEEYAYKIGADYYGKTAQDAVKIADKFFLKNAL LCKTCGI
Sequences:
>Translated_807_residues MLDIFKELSNRVIVFDGAMGTQLQERGLKTGECPEYMNITHPEVVFDIHRSYIEAGADVIETNTFGANRIKLAKYGLENE VFNIVTQAVKIAKEASKDKPVALSIGPIGELLTPYGDMTFDEAYDVFKEVVIAAERAGADIVLIETMSDMLEAKAAILAA KENSNMKVICTMTFQEDGRTLMGSDPITVVVSLQGLGLDAIGVNCSTGPDKMVSVVEKMSQVSRIPIIAQPNAGMPVIRD GKTVYDLKPEEFASFFPSLVEKGASIVGGCCGTTPHYIKLVKKAVKDLKPKVKVNKFTAVASNTKTVFIGHDYSLRVIGE RINPTGKKKLSEAFLAGNVSLAVEEAIKQQKCGAEILDVNVGVPGVNEEELLPKVVSEIQNVVDIPLQIDSTNIKAVEKA IRILRGRPIINSVSAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERIENAKKIIKKAEEYGIPKEDILIDCIVLT VSSEQEAAIETLEAIKLAKEELGANTVVGLSNVSFGLPERRLINSTFLAMAASYGLTTAIINPCDEAMMDTLRASMVLLN KDKGSVNYLKIYGNRQKEEGKEKEQQKIQEEDLKSKFYIQILEGKKSGVEDIVKNILEKEVQPLSIVDNIIIPALKEVGD RYEKGIYFLPQLLSSAEVVQNAFRIIKEKLPKGSVSKGKIILATVEGDVHDIGKNIVKVLLENYGYDVIDLGKDVKGEVI LEEVKRTGAPLVGLSALMTTTLFNMEKIIKLLKANTDVKIMVGGAVLTEEYAYKIGADYYGKTAQDAVKIADKFFLKNAL LCKTCGI >Mature_807_residues MLDIFKELSNRVIVFDGAMGTQLQERGLKTGECPEYMNITHPEVVFDIHRSYIEAGADVIETNTFGANRIKLAKYGLENE VFNIVTQAVKIAKEASKDKPVALSIGPIGELLTPYGDMTFDEAYDVFKEVVIAAERAGADIVLIETMSDMLEAKAAILAA KENSNMKVICTMTFQEDGRTLMGSDPITVVVSLQGLGLDAIGVNCSTGPDKMVSVVEKMSQVSRIPIIAQPNAGMPVIRD GKTVYDLKPEEFASFFPSLVEKGASIVGGCCGTTPHYIKLVKKAVKDLKPKVKVNKFTAVASNTKTVFIGHDYSLRVIGE RINPTGKKKLSEAFLAGNVSLAVEEAIKQQKCGAEILDVNVGVPGVNEEELLPKVVSEIQNVVDIPLQIDSTNIKAVEKA IRILRGRPIINSVSAKEESLKEVLPIVKKYGACVVGLTVGDKGLPKDRHERIENAKKIIKKAEEYGIPKEDILIDCIVLT VSSEQEAAIETLEAIKLAKEELGANTVVGLSNVSFGLPERRLINSTFLAMAASYGLTTAIINPCDEAMMDTLRASMVLLN KDKGSVNYLKIYGNRQKEEGKEKEQQKIQEEDLKSKFYIQILEGKKSGVEDIVKNILEKEVQPLSIVDNIIIPALKEVGD RYEKGIYFLPQLLSSAEVVQNAFRIIKEKLPKGSVSKGKIILATVEGDVHDIGKNIVKVLLENYGYDVIDLGKDVKGEVI LEEVKRTGAPLVGLSALMTTTLFNMEKIIKLLKANTDVKIMVGGAVLTEEYAYKIGADYYGKTAQDAVKIADKFFLKNAL LCKTCGI
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=863, Percent_Identity=30.0115874855156, Blast_Score=311, Evalue=1e-84, Organism=Homo sapiens, GI157266337, Length=300, Percent_Identity=26, Blast_Score=70, Evalue=8e-12, Organism=Escherichia coli, GI1790450, Length=848, Percent_Identity=30.188679245283, Blast_Score=342, Evalue=4e-95, Organism=Escherichia coli, GI1786456, Length=297, Percent_Identity=26.9360269360269, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17535405, Length=868, Percent_Identity=29.6082949308756, Blast_Score=306, Evalue=3e-83, Organism=Drosophila melanogaster, GI24585077, Length=297, Percent_Identity=25.9259259259259, Blast_Score=78, Evalue=3e-14,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 88139; Mature: 88139
Theoretical pI: Translated: 5.78; Mature: 5.78
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLDIFKELSNRVIVFDGAMGTQLQERGLKTGECPEYMNITHPEVVFDIHRSYIEAGADVI CCCHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE ETNTFGANRIKLAKYGLENEVFNIVTQAVKIAKEASKDKPVALSIGPIGELLTPYGDMTF ECCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCH DEAYDVFKEVVIAAERAGADIVLIETMSDMLEAKAAILAAKENSNMKVICTMTFQEDGRT HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCE LMGSDPITVVVSLQGLGLDAIGVNCSTGPDKMVSVVEKMSQVSRIPIIAQPNAGMPVIRD EECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEC GKTVYDLKPEEFASFFPSLVEKGASIVGGCCGTTPHYIKLVKKAVKDLKPKVKVNKFTAV CCEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEE ASNTKTVFIGHDYSLRVIGERINPTGKKKLSEAFLAGNVSLAVEEAIKQQKCGAEILDVN ECCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE VGVPGVNEEELLPKVVSEIQNVVDIPLQIDSTNIKAVEKAIRILRGRPIINSVSAKEESL CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH KEVLPIVKKYGACVVGLTVGDKGLPKDRHERIENAKKIIKKAEEYGIPKEDILIDCIVLT HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEE VSSEQEAAIETLEAIKLAKEELGANTVVGLSNVSFGLPERRLINSTFLAMAASYGLTTAI ECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHH INPCDEAMMDTLRASMVLLNKDKGSVNYLKIYGNRQKEEGKEKEQQKIQEEDLKSKFYIQ HCCHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHEEEE ILEGKKSGVEDIVKNILEKEVQPLSIVDNIIIPALKEVGDRYEKGIYFLPQLLSSAEVVQ EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH NAFRIIKEKLPKGSVSKGKIILATVEGDVHDIGKNIVKVLLENYGYDVIDLGKDVKGEVI HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH LEEVKRTGAPLVGLSALMTTTLFNMEKIIKLLKANTDVKIMVGGAVLTEEYAYKIGADYY HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCC GKTAQDAVKIADKFFLKNALLCKTCGI CCCHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MLDIFKELSNRVIVFDGAMGTQLQERGLKTGECPEYMNITHPEVVFDIHRSYIEAGADVI CCCHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE ETNTFGANRIKLAKYGLENEVFNIVTQAVKIAKEASKDKPVALSIGPIGELLTPYGDMTF ECCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCH DEAYDVFKEVVIAAERAGADIVLIETMSDMLEAKAAILAAKENSNMKVICTMTFQEDGRT HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCE LMGSDPITVVVSLQGLGLDAIGVNCSTGPDKMVSVVEKMSQVSRIPIIAQPNAGMPVIRD EECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEC GKTVYDLKPEEFASFFPSLVEKGASIVGGCCGTTPHYIKLVKKAVKDLKPKVKVNKFTAV CCEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEE ASNTKTVFIGHDYSLRVIGERINPTGKKKLSEAFLAGNVSLAVEEAIKQQKCGAEILDVN ECCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE VGVPGVNEEELLPKVVSEIQNVVDIPLQIDSTNIKAVEKAIRILRGRPIINSVSAKEESL CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH KEVLPIVKKYGACVVGLTVGDKGLPKDRHERIENAKKIIKKAEEYGIPKEDILIDCIVLT HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEE VSSEQEAAIETLEAIKLAKEELGANTVVGLSNVSFGLPERRLINSTFLAMAASYGLTTAI ECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHH INPCDEAMMDTLRASMVLLNKDKGSVNYLKIYGNRQKEEGKEKEQQKIQEEDLKSKFYIQ HCCHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHEEEE ILEGKKSGVEDIVKNILEKEVQPLSIVDNIIIPALKEVGDRYEKGIYFLPQLLSSAEVVQ EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH NAFRIIKEKLPKGSVSKGKIILATVEGDVHDIGKNIVKVLLENYGYDVIDLGKDVKGEVI HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH LEEVKRTGAPLVGLSALMTTTLFNMEKIIKLLKANTDVKIMVGGAVLTEEYAYKIGADYY HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCC GKTAQDAVKIADKFFLKNALLCKTCGI CCCHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230 [H]