Click here to switch to the map view.

The map label for this gene is yitJ [H]

Identifier: 167039855

GI number: 167039855

Start: 1251401

End: 1252312

Strand: Direct

Name: yitJ [H]

Synonym: Teth514_1210

Alternate gene names: 167039855

Gene position: 1251401-1252312 (Clockwise)

Preceding gene: 167039853

Following gene: 167039856

Centisome position: 50.93

GC content: 35.42

Gene sequence:

>912_bases
ATGTTGAAAAATGAAAATTTGTGTACTAAACTTAAGAAAAGGAAGTTTGTTGTGACAGCAGAAATTTCTACGCCAAAAGG
GGTAGATATAACGAAAACTATTGAAGAAGCGAGAAAATTAAAAGGCATTGTAGATGCCTTAAATATAACGGACTGTCCTA
TGGCGAATATGCGGATGAGTCCTATAGCAGTCGCTCACCTTTTACAAGACCACTTAAACATTGAAGCTATTTTTCATTTG
ACCTGTAGAGACAGAAATCTGATAGGCCTTCAGTCAGAGCTTTTAGGAGCTTATGCATTAGGGGTGAGAAATATACTTGC
ATTGACAGGAGATGACCCTTCCCGTGGAGATACTCCTCAGGCTAAAGGGGTATATGATGTAGATTCTATTGGGCTTTTAA
AGATTATCAGTGCTTTTAATAGAGGTTATGATTACAATGGAAATAAATTAGATTCCCCAACAGAATTTTGCGCCGGTACG
ACTGCAAATCCAAATGATTTAAGGGATGAAGCACTAGAGAAGCTTAAAGTTAAGATAGAGGCAGGGGCAGAATTTATTCA
AACACAACCGGTTTATGATGAGGAAATTTTATTTAAGTTTATTGAAAAGATTAAAAATTTCAATGTACCACTTTTGATAG
GCATATTACCTCTTAAAAGCTATAAAATGGCAGTCAATTTAAATGAAAAAGTGCCAGGTATAGATATTCCAGAAAAAGTT
TTAGAAAGGCTAAAGGATGGAGGAAGAGAAGAGGGAATAAACATAGCTGTAGAGTTTATAAAGCGGATACGCAGTGAAGT
TGCAGGAATTCACATTATGCCTTTAGGGAGTACAAATGTTGTGTTAGAAATAATTTCAAGAGTCGATGATTTAGTCTGTG
ATTTTGCAGAAAATAACGTACAGATGAATTAA

Upstream 100 bases:

>100_bases
GTGCCAATTCCAGCAGCGTTTCGCTGAAAGATGAGAGAGTTTTGGTGTCTCTTCTTTTGGCGAAGAGACTTTTTTATTTT
TAAAAATGGAGGCGATGTAG

Downstream 100 bases:

>100_bases
ATGGAGGTTAAAAATGTTAGACATTTTTAAAGAACTTTCAAATAGAGTGATTGTTTTTGATGGAGCAATGGGGACACAAC
TTCAAGAGAGGGGTTTAAAA

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLKNENLCTKLKKRKFVVTAEISTPKGVDITKTIEEARKLKGIVDALNITDCPMANMRMSPIAVAHLLQDHLNIEAIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDDPSRGDTPQAKGVYDVDSIGLLKIISAFNRGYDYNGNKLDSPTEFCAGT
TANPNDLRDEALEKLKVKIEAGAEFIQTQPVYDEEILFKFIEKIKNFNVPLLIGILPLKSYKMAVNLNEKVPGIDIPEKV
LERLKDGGREEGINIAVEFIKRIRSEVAGIHIMPLGSTNVVLEIISRVDDLVCDFAENNVQMN

Sequences:

>Translated_303_residues
MLKNENLCTKLKKRKFVVTAEISTPKGVDITKTIEEARKLKGIVDALNITDCPMANMRMSPIAVAHLLQDHLNIEAIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDDPSRGDTPQAKGVYDVDSIGLLKIISAFNRGYDYNGNKLDSPTEFCAGT
TANPNDLRDEALEKLKVKIEAGAEFIQTQPVYDEEILFKFIEKIKNFNVPLLIGILPLKSYKMAVNLNEKVPGIDIPEKV
LERLKDGGREEGINIAVEFIKRIRSEVAGIHIMPLGSTNVVLEIISRVDDLVCDFAENNVQMN
>Mature_303_residues
MLKNENLCTKLKKRKFVVTAEISTPKGVDITKTIEEARKLKGIVDALNITDCPMANMRMSPIAVAHLLQDHLNIEAIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDDPSRGDTPQAKGVYDVDSIGLLKIISAFNRGYDYNGNKLDSPTEFCAGT
TANPNDLRDEALEKLKVKIEAGAEFIQTQPVYDEEILFKFIEKIKNFNVPLLIGILPLKSYKMAVNLNEKVPGIDIPEKV
LERLKDGGREEGINIAVEFIKRIRSEVAGIHIMPLGSTNVVLEIISRVDDLVCDFAENNVQMN

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325234, Length=305, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 33631; Mature: 33631

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNENLCTKLKKRKFVVTAEISTPKGVDITKTIEEARKLKGIVDALNITDCPMANMRMS
CCCCCHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
PIAVAHLLQDHLNIEAIFHLTCRDRNLIGLQSELLGAYALGVRNILALTGDDPSRGDTPQ
HHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
AKGVYDVDSIGLLKIISAFNRGYDYNGNKLDSPTEFCAGTTANPNDLRDEALEKLKVKIE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
AGAEFIQTQPVYDEEILFKFIEKIKNFNVPLLIGILPLKSYKMAVNLNEKVPGIDIPEKV
HCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHH
LERLKDGGREEGINIAVEFIKRIRSEVAGIHIMPLGSTNVVLEIISRVDDLVCDFAENNV
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
QMN
CCC
>Mature Secondary Structure
MLKNENLCTKLKKRKFVVTAEISTPKGVDITKTIEEARKLKGIVDALNITDCPMANMRMS
CCCCCHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
PIAVAHLLQDHLNIEAIFHLTCRDRNLIGLQSELLGAYALGVRNILALTGDDPSRGDTPQ
HHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
AKGVYDVDSIGLLKIISAFNRGYDYNGNKLDSPTEFCAGTTANPNDLRDEALEKLKVKIE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
AGAEFIQTQPVYDEEILFKFIEKIKNFNVPLLIGILPLKSYKMAVNLNEKVPGIDIPEKV
HCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHH
LERLKDGGREEGINIAVEFIKRIRSEVAGIHIMPLGSTNVVLEIISRVDDLVCDFAENNV
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
QMN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]