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The map label for this gene is thiH [C]

Identifier: 167039779

GI number: 167039779

Start: 1171258

End: 1172316

Strand: Reverse

Name: thiH [C]

Synonym: Teth514_1130

Alternate gene names: 167039779

Gene position: 1172316-1171258 (Counterclockwise)

Preceding gene: 167039799

Following gene: 167039773

Centisome position: 47.71

GC content: 32.86

Gene sequence:

>1059_bases
GTGATATTCATGATAAAAGAAAAAGATATTGATGAATTAATTGAAAAATTAGAAACACATCACAACATTGATAAAGAAGA
ACTTGTGACACTTTTAGCTTTAGAAGACCCTTCCAAGATTTATCAAGCTGCGGATAGAGTTAGAAAAAAATATGTAGGAG
ATGAAGTACATTTAAGAGGTCTTATAGAATTCAGTAACTACTGCAGCAATACTTGTTTTTATTGTGGCCTTCGAGGTCCT
AACAGGACAATTAAACGATACAGAATGGAACCCGAAGAAATCTTACAATGTGTAAAATATGGAGCATCAGTTGGACTAAA
AACAATAGTCCTTCAAAGCGGCGAGGATAAATATTTTAAAATAACTACTTTATGTAAAATAATAGAAGAAATTAAAAAAT
TAGATATTGCAGTAACTTTAAGTATTGGAGAACTTTCCACTAAAGACTACGCTGAATTAAAAAAAGCAGGAGCCGATAGA
TATCTACTTCGCATTGAAACTACAAACAAAGAGCTTTATCAAAAATTACATCCCGGCATGAGTTATGAAAATCGCGTAAG
GTGCCTTATGGATTTAAGAGAATTAGGTTATGAAGTCGGAACAGGAAGCCTTGTAGGCCTCCCGGGACAAACTTTAGAAA
TGCTGGCACAAGACCTCATATTCTTTAAAAAAATAGATGCAGATATGCTAGGAATAGGTCCTTTTATACCTTGCGAAAAC
ACACCTTTAGAAAGGGAAAAAGGCGGCAATGTGGAAATTGTATTAAAAATGTTAGCTTTATCAAGACTACTATTGCCTGA
CATTAACATACCTGCTACTACTGCTTTGGCTGTTAAAGACAAAGCAGGTTATATTAAAGGACTAAAGTGTGGAGCTAATG
TAATAATGCCAAATATAGGTATAGATGAGTATAAAAAATTATATAAACTTTATCCTGGAAAAGTACCTGACAATCCCTCT
GAAGCTGTAAATAGCCTTGAAAATATCAAAAAGCTTATTTTATCCCAAAATAGGACAATTGGAAAAGATAAAGGCTATCG
TAAAAAAATATTAAATTGA

Upstream 100 bases:

>100_bases
CTTATGTCAATAAAATATCAAAAATTTGTTAAGAAAATAGTTTAACAATTTTATTGATTTTTTATTAACAATTGTTTTAT
ACTAATTAGGGAAAGAGAGA

Downstream 100 bases:

>100_bases
TATACGATAGCCTTTTTTCTATGAAATTGCTCCTACAGGGCAAATATCAATACATCTGCCACAGTCTCTGCACTTCTTAG
GGTCTATATAGGCAATTCCA

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIFMIKEKDIDELIEKLETHHNIDKEELVTLLALEDPSKIYQAADRVRKKYVGDEVHLRGLIEFSNYCSNTCFYCGLRGP
NRTIKRYRMEPEEILQCVKYGASVGLKTIVLQSGEDKYFKITTLCKIIEEIKKLDIAVTLSIGELSTKDYAELKKAGADR
YLLRIETTNKELYQKLHPGMSYENRVRCLMDLRELGYEVGTGSLVGLPGQTLEMLAQDLIFFKKIDADMLGIGPFIPCEN
TPLEREKGGNVEIVLKMLALSRLLLPDINIPATTALAVKDKAGYIKGLKCGANVIMPNIGIDEYKKLYKLYPGKVPDNPS
EAVNSLENIKKLILSQNRTIGKDKGYRKKILN

Sequences:

>Translated_352_residues
MIFMIKEKDIDELIEKLETHHNIDKEELVTLLALEDPSKIYQAADRVRKKYVGDEVHLRGLIEFSNYCSNTCFYCGLRGP
NRTIKRYRMEPEEILQCVKYGASVGLKTIVLQSGEDKYFKITTLCKIIEEIKKLDIAVTLSIGELSTKDYAELKKAGADR
YLLRIETTNKELYQKLHPGMSYENRVRCLMDLRELGYEVGTGSLVGLPGQTLEMLAQDLIFFKKIDADMLGIGPFIPCEN
TPLEREKGGNVEIVLKMLALSRLLLPDINIPATTALAVKDKAGYIKGLKCGANVIMPNIGIDEYKKLYKLYPGKVPDNPS
EAVNSLENIKKLILSQNRTIGKDKGYRKKILN
>Mature_352_residues
MIFMIKEKDIDELIEKLETHHNIDKEELVTLLALEDPSKIYQAADRVRKKYVGDEVHLRGLIEFSNYCSNTCFYCGLRGP
NRTIKRYRMEPEEILQCVKYGASVGLKTIVLQSGEDKYFKITTLCKIIEEIKKLDIAVTLSIGELSTKDYAELKKAGADR
YLLRIETTNKELYQKLHPGMSYENRVRCLMDLRELGYEVGTGSLVGLPGQTLEMLAQDLIFFKKIDADMLGIGPFIPCEN
TPLEREKGGNVEIVLKMLALSRLLLPDINIPATTALAVKDKAGYIKGLKCGANVIMPNIGIDEYKKLYKLYPGKVPDNPS
EAVNSLENIKKLILSQNRTIGKDKGYRKKILN

Specific function: Required For The Synthesis Of The Thiazole Moiety Of Thiamine. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 39830; Mature: 39830

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFMIKEKDIDELIEKLETHHNIDKEELVTLLALEDPSKIYQAADRVRKKYVGDEVHLRG
CEEEEECCCHHHHHHHHHHHCCCCHHHHEEHHEECCHHHHHHHHHHHHHHHCCCHHHHHH
LIEFSNYCSNTCFYCGLRGPNRTIKRYRMEPEEILQCVKYGASVGLKTIVLQSGEDKYFK
HHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEECCCCCEEH
ITTLCKIIEEIKKLDIAVTLSIGELSTKDYAELKKAGADRYLLRIETTNKELYQKLHPGM
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCC
SYENRVRCLMDLRELGYEVGTGSLVGLPGQTLEMLAQDLIFFKKIDADMLGIGPFIPCEN
CHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TPLEREKGGNVEIVLKMLALSRLLLPDINIPATTALAVKDKAGYIKGLKCGANVIMPNIG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCEEECCCC
IDEYKKLYKLYPGKVPDNPSEAVNSLENIKKLILSQNRTIGKDKGYRKKILN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MIFMIKEKDIDELIEKLETHHNIDKEELVTLLALEDPSKIYQAADRVRKKYVGDEVHLRG
CEEEEECCCHHHHHHHHHHHCCCCHHHHEEHHEECCHHHHHHHHHHHHHHHCCCHHHHHH
LIEFSNYCSNTCFYCGLRGPNRTIKRYRMEPEEILQCVKYGASVGLKTIVLQSGEDKYFK
HHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEECCCCCEEH
ITTLCKIIEEIKKLDIAVTLSIGELSTKDYAELKKAGADRYLLRIETTNKELYQKLHPGM
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCC
SYENRVRCLMDLRELGYEVGTGSLVGLPGQTLEMLAQDLIFFKKIDADMLGIGPFIPCEN
CHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TPLEREKGGNVEIVLKMLALSRLLLPDINIPATTALAVKDKAGYIKGLKCGANVIMPNIG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCEEECCCC
IDEYKKLYKLYPGKVPDNPSEAVNSLENIKKLILSQNRTIGKDKGYRKKILN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]