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The map label for this gene is regA [H]

Identifier: 167039722

GI number: 167039722

Start: 1108512

End: 1109504

Strand: Direct

Name: regA [H]

Synonym: Teth514_1072

Alternate gene names: 167039722

Gene position: 1108512-1109504 (Clockwise)

Preceding gene: 167039721

Following gene: 167039723

Centisome position: 45.11

GC content: 35.75

Gene sequence:

>993_bases
ATGGCTAATATTAGAGAAGTTGCTAAAAGAGCAGGAGTCTCTGTTGCAACAGTATCAAGAGTTTTAAATGGCAGTGACTC
GGTGTCTGAAGAAACAAAGGAAAGAGTATTAAAAGCGATAAAAGAATTAAATTATCATCCAAATCTCCTTGGAAGAAATT
TAAGACGTTCTGAAACGAAAATGATATTGGCTTTGATGCCTAATGTCTCAAATCCTTTTTATGCAAGGGTTGTAAAAGGA
ATAGAAGATGTAGCCCATAAAAATGGTTATAATGTAATGCTTTGCAATACTGATTCTGATATTAACAGAGAAAAGGTATA
CCTTGAAATTTTAAAAAACAGATTAGCGGATGGCGTTATTTTTATGGCGCCTGTGATGGGAAAAGAAGAATTGACATTGA
TAGGCCAAAATTATCCTGTCGTTCAGTGTTGTGAATACATAGAAGGAGCTGGCGTTTCATATGTATCTATAGACAACTTT
TCTGCAGCTTACAAAGCAGTAAAGCACTTGGTAAGTTTAGGACATAAAAAAATAGGAATGATAAGCTGTGAAAATAACTT
TGTATCTACGAAGCAGAGGGAAGCGAGTTATAAAAAAGCTCTTGAAGATTCAGGGATAGAATTTGACGAAAAGCTTATAA
AATACGGAGATTACAGCTTTAAAAGCGGAGTAAGAGCAGCGAAACAGCTTTTGGTGATGGAAGAAAGGCCTACTGCTATA
TTTGCAATCTCTGACATCATGGCTATTGGAGCTATAAGAGCGATAAAGGAAGAGGGGCTTAAAGTCCCTGAGGATATAGC
GGTTGTAGGCTTTGATGATATAAGCTTTGCTTCTATGTATGACCCTATGCTTACAACTATTTCACAGCCAAAATACGATT
TAGGATGTGTTGCTATGGAACTACTGATAAAACACATGAGTGGCAAATTAGAAGAACCACAGAGGATTTTTTTAGAACAT
GAGTTAATAATAAGAGAGTCGACAGTAAAATAA

Upstream 100 bases:

>100_bases
ACCGCCTGCTGGGAATAGGCACTCTCTGAAAGGGCGGACTATAAATAGCCCAGCCTTCTAAACTAGTGCAGTTTTGCACA
GTTTAGATGACCAGGTTACA

Downstream 100 bases:

>100_bases
AGAATAAAGTTTTGTCTTAAAAATACAAATTTAAATATAATCATGGGAGGAATTTTTATGGTAAAAGAAAAACTTAGAGT
AGGAATTATAGGTTGTGGAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MANIREVAKRAGVSVATVSRVLNGSDSVSEETKERVLKAIKELNYHPNLLGRNLRRSETKMILALMPNVSNPFYARVVKG
IEDVAHKNGYNVMLCNTDSDINREKVYLEILKNRLADGVIFMAPVMGKEELTLIGQNYPVVQCCEYIEGAGVSYVSIDNF
SAAYKAVKHLVSLGHKKIGMISCENNFVSTKQREASYKKALEDSGIEFDEKLIKYGDYSFKSGVRAAKQLLVMEERPTAI
FAISDIMAIGAIRAIKEEGLKVPEDIAVVGFDDISFASMYDPMLTTISQPKYDLGCVAMELLIKHMSGKLEEPQRIFLEH
ELIIRESTVK

Sequences:

>Translated_330_residues
MANIREVAKRAGVSVATVSRVLNGSDSVSEETKERVLKAIKELNYHPNLLGRNLRRSETKMILALMPNVSNPFYARVVKG
IEDVAHKNGYNVMLCNTDSDINREKVYLEILKNRLADGVIFMAPVMGKEELTLIGQNYPVVQCCEYIEGAGVSYVSIDNF
SAAYKAVKHLVSLGHKKIGMISCENNFVSTKQREASYKKALEDSGIEFDEKLIKYGDYSFKSGVRAAKQLLVMEERPTAI
FAISDIMAIGAIRAIKEEGLKVPEDIAVVGFDDISFASMYDPMLTTISQPKYDLGCVAMELLIKHMSGKLEEPQRIFLEH
ELIIRESTVK
>Mature_329_residues
ANIREVAKRAGVSVATVSRVLNGSDSVSEETKERVLKAIKELNYHPNLLGRNLRRSETKMILALMPNVSNPFYARVVKGI
EDVAHKNGYNVMLCNTDSDINREKVYLEILKNRLADGVIFMAPVMGKEELTLIGQNYPVVQCCEYIEGAGVSYVSIDNFS
AAYKAVKHLVSLGHKKIGMISCENNFVSTKQREASYKKALEDSGIEFDEKLIKYGDYSFKSGVRAAKQLLVMEERPTAIF
AISDIMAIGAIRAIKEEGLKVPEDIAVVGFDDISFASMYDPMLTTISQPKYDLGCVAMELLIKHMSGKLEEPQRIFLEHE
LIIRESTVK

Specific function: Involved in the regulation of amylase production [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=37.8048780487805, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=36.0606060606061, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=35.7575757575758, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788474, Length=312, Percent_Identity=33.6538461538462, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=31.2312312312312, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=32.5342465753425, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787580, Length=331, Percent_Identity=29.0030211480363, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786540, Length=327, Percent_Identity=30.2752293577982, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=26.875, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=28.4457478005865, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787906, Length=334, Percent_Identity=24.8502994011976, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=25.3246753246753, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=22.7414330218069, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36840; Mature: 36709

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIREVAKRAGVSVATVSRVLNGSDSVSEETKERVLKAIKELNYHPNLLGRNLRRSETK
CCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHH
MILALMPNVSNPFYARVVKGIEDVAHKNGYNVMLCNTDSDINREKVYLEILKNRLADGVI
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEE
FMAPVMGKEELTLIGQNYPVVQCCEYIEGAGVSYVSIDNFSAAYKAVKHLVSLGHKKIGM
EEECCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEE
ISCENNFVSTKQREASYKKALEDSGIEFDEKLIKYGDYSFKSGVRAAKQLLVMEERPTAI
EEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEE
FAISDIMAIGAIRAIKEEGLKVPEDIAVVGFDDISFASMYDPMLTTISQPKYDLGCVAME
EHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LLIKHMSGKLEEPQRIFLEHELIIRESTVK
HHHHHHCCCCCCHHHHHHHHHHHEECCCCC
>Mature Secondary Structure 
ANIREVAKRAGVSVATVSRVLNGSDSVSEETKERVLKAIKELNYHPNLLGRNLRRSETK
CCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHH
MILALMPNVSNPFYARVVKGIEDVAHKNGYNVMLCNTDSDINREKVYLEILKNRLADGVI
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEE
FMAPVMGKEELTLIGQNYPVVQCCEYIEGAGVSYVSIDNFSAAYKAVKHLVSLGHKKIGM
EEECCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEE
ISCENNFVSTKQREASYKKALEDSGIEFDEKLIKYGDYSFKSGVRAAKQLLVMEERPTAI
EEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEE
FAISDIMAIGAIRAIKEEGLKVPEDIAVVGFDDISFASMYDPMLTTISQPKYDLGCVAME
EHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LLIKHMSGKLEEPQRIFLEHELIIRESTVK
HHHHHHCCCCCCHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7539689 [H]