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The map label for this gene is glpF [H]

Identifier: 167039669

GI number: 167039669

Start: 1048994

End: 1049722

Strand: Direct

Name: glpF [H]

Synonym: Teth514_1019

Alternate gene names: 167039669

Gene position: 1048994-1049722 (Clockwise)

Preceding gene: 167039668

Following gene: 167039670

Centisome position: 42.69

GC content: 41.02

Gene sequence:

>729_bases
ATGAGTGACCTTGCAAAATATGTTGCTGAATTTTTTGGTACTATGATACTTATTTGGTTAGGTGATGGCGTTGTTGCAAA
CGTTGTCTTGAATAAATCTAAAGGGCAAAATAGCGGCTGGATTGTAATCACAGCGGGATGGGGATTTGCAGTAATGGTAG
CTGTTTATGTAGTTGGATGGATAAGTGGTGCTCACATAAATCCAGCTGTGACTCTTGGTTTAGCAACGATAGGGAACTTT
CCATGGAGTTTAGTGCCAGGCTACATTATAGCACAAGTTTTGGGAGCTTTTGTGGGAGCCATAATTGTTTATTTGATGTA
CATGGATCATTTTGCTGCAACTGATGATCCCACAGCTAAGCTTGGTACTTTTGCAACTATTCCAGCTATAAGAAATTTGG
GTAAGAATTTTATGACAGAGGCATTTGGTACTGCCATGCTTTTAATAGGAATACTTGGAATTACTAATAGCAATAACTCT
GTTGGAGGAATGGGAGCGCTTTTAGTAGGACTTCTCGTATGGGCGATAGGTTTAAGCTTAGGTGGACCTACAGGTTATGC
AATTAATCCGGCGAGAGACTTCGGCCCGAGACTAGCTCATGCTGTTCTTCCTATTCCAGGTAAAGGGGATTCTGATTGGG
GATACGGCTTAATAGTTCCAATATTTGGGCCGATAGTCGGAGGAATACTTGGAGCCATAGTTTACCAACTTATTGTGAAT
ATACATTAG

Upstream 100 bases:

>100_bases
TCTGTGGTTTATAGAATGAAAGGAGGGATAGTTTTAACATATTGCATAATTATTTTAAAAGTTAAACGATTTGAAATTTG
GAATTATGGAGGGATATTTT

Downstream 100 bases:

>100_bases
TGAAAGAGCTTAAATCGGCGAGAGTTTTTCTCTCGCCGACAAATATAACAAAAATTATGAGGGGGAAAAGAGATGGCAAA
GTACATTATGGCTTTTGACC

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSDLAKYVAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVAVYVVGWISGAHINPAVTLGLATIGNF
PWSLVPGYIIAQVLGAFVGAIIVYLMYMDHFAATDDPTAKLGTFATIPAIRNLGKNFMTEAFGTAMLLIGILGITNSNNS
VGGMGALLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAVLPIPGKGDSDWGYGLIVPIFGPIVGGILGAIVYQLIVN
IH

Sequences:

>Translated_242_residues
MSDLAKYVAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVAVYVVGWISGAHINPAVTLGLATIGNF
PWSLVPGYIIAQVLGAFVGAIIVYLMYMDHFAATDDPTAKLGTFATIPAIRNLGKNFMTEAFGTAMLLIGILGITNSNNS
VGGMGALLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAVLPIPGKGDSDWGYGLIVPIFGPIVGGILGAIVYQLIVN
IH
>Mature_241_residues
SDLAKYVAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVAVYVVGWISGAHINPAVTLGLATIGNFP
WSLVPGYIIAQVLGAFVGAIIVYLMYMDHFAATDDPTAKLGTFATIPAIRNLGKNFMTEAFGTAMLLIGILGITNSNNSV
GGMGALLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAVLPIPGKGDSDWGYGLIVPIFGPIVGGILGAIVYQLIVNI
H

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=257, Percent_Identity=40.8560311284047, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI157266307, Length=262, Percent_Identity=39.3129770992366, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI4502187, Length=250, Percent_Identity=36.8, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI22538420, Length=252, Percent_Identity=35.7142857142857, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=37.5690607734807, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=37.5690607734807, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI4502179, Length=236, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4755125, Length=234, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502181, Length=234, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI37694062, Length=244, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=253, Percent_Identity=37.9446640316205, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787101, Length=190, Percent_Identity=32.6315789473684, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=34.136546184739, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=34.6938775510204, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=34.6938775510204, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=252, Percent_Identity=36.5079365079365, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=33.6032388663968, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=248, Percent_Identity=34.2741935483871, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=248, Percent_Identity=34.2741935483871, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=31.0204081632653, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=239, Percent_Identity=27.1966527196653, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=240, Percent_Identity=27.5, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=198, Percent_Identity=39.3939393939394, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322985, Length=285, Percent_Identity=29.1228070175439, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=30.6382978723404, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=30.6382978723404, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550503, Length=241, Percent_Identity=24.4813278008299, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25229; Mature: 25098

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLAKYVAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVAVYVVGW
CCHHHHHHHHHHHHEEHHHHCCCCEEHHEEECCCCCCCCEEEEECCHHHHHHHHHHHHHH
ISGAHINPAVTLGLATIGNFPWSLVPGYIIAQVLGAFVGAIIVYLMYMDHFAATDDPTAK
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LGTFATIPAIRNLGKNFMTEAFGTAMLLIGILGITNSNNSVGGMGALLVGLLVWAIGLSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
GGPTGYAINPARDFGPRLAHAVLPIPGKGDSDWGYGLIVPIFGPIVGGILGAIVYQLIVN
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
IH
CC
>Mature Secondary Structure 
SDLAKYVAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVAVYVVGW
CHHHHHHHHHHHHEEHHHHCCCCEEHHEEECCCCCCCCEEEEECCHHHHHHHHHHHHHH
ISGAHINPAVTLGLATIGNFPWSLVPGYIIAQVLGAFVGAIIVYLMYMDHFAATDDPTAK
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LGTFATIPAIRNLGKNFMTEAFGTAMLLIGILGITNSNNSVGGMGALLVGLLVWAIGLSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
GGPTGYAINPARDFGPRLAHAVLPIPGKGDSDWGYGLIVPIFGPIVGGILGAIVYQLIVN
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
IH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]