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The map label for this gene is gltX [H]

Identifier: 167039498

GI number: 167039498

Start: 881351

End: 882808

Strand: Direct

Name: gltX [H]

Synonym: Teth514_0844

Alternate gene names: 167039498

Gene position: 881351-882808 (Clockwise)

Preceding gene: 167039497

Following gene: 167039499

Centisome position: 35.87

GC content: 33.81

Gene sequence:

>1458_bases
ATGAACAATTTGAGAGTTAGATTTGCGCCAAGCCCAACTGGAGCTATTCATATAGGGAATATAAGAACTGCTTTATTTAA
TTATCTTTTTTCAAGAAGTGAAGGGGCTACTTTTGTTTTAAGGATTGAAGACACGGACTTAGAAAGGTCTTCAAAAGAGT
TTGAAGAATTAATTTTTAAAGAATTAGAGTGGTTAGGTATAGAATGGGACGAAGGACCAGATAAACCAGGTCCCTATGGC
CCTTATAGGCAAAGTGAAAGACTTGAAATTTATCATAAATTTGCCCAGAAACTTATTGAAGAAAAAAAGGCATATAGATG
TTATTGTACTCCGGAGGAATTAGAAGAAGATAGAAGAAAGGCAGTAGAAAGAGGAGATATACCTCGTTATTCTGGAAGGT
GTAGGTATCTTACAAAGGAGCAAGAAGAGGCTTTTATAAGAGAGGGAAGAAAACCTGTAATAAGGTTTATTATTCCTGAC
GATGAAGTTATAGAGTTTGAAGACATGATAAAAGGAAAAATTACAATAAAAAGTGATACTTTAGGCGGGGACATGGTAAT
TGTCAAATCAGACGGAATGCCTACTTACAATTTTGCAGTTGTAATAGATGACGCTCTTATGAAGATTACCCATGTCATAA
GAGGAGAAGACCATATATACAATACTCCGAAACAGATACTCATTTACAAGGCTTTAGGATTTGAAATTCCTAAGTTTGCC
CATGCGCCTTTAATATTGGGACCAGATAGGACTAAACTTTCTAAAAGGCATGGGAATACTTATATAGGCCAGTATAGAGA
ATTGGGATATTTGCCAGAAGCTATGTTTAACTTTTTATCTCTTTTAAGTTGGTCACCGGAGGATAATGTAGAAATCATGT
CAAAAGAAGAAATAATAAAGAAATTTAATTTCAGAAGGATTCACAGTGCTAATCCTGTCTTTGATATTGAAAAGCTTAAT
TGGATGAATCAACAGTATATACAAAAAAGTTCTATTGAGAGAATTGTAGATTTGGCTATTCCACATTTAAGAAGGGCAGG
ATACATTGACGGAATAGATGATATGGTGTATAATTGGCTAAAGGATGTAATATCCCTTTACAAAGATGGACTCCAATATG
TGGCTCAAATTACTGAGAAAGCTAAGATGTTTTTTGTAGAAGAAGTTGAGTATTCTGATGAAACGGTGAAAATATTGAAT
AGCCCAAATAGTAAAATTGTACTAGAAGTAGTTAAAAAAGTCATAGAGGAAGCTGATGAAATAACAGAGGAATATGTTAA
AGATTTGCTTAAAAAATTGCAAAAAGAAACCAAGGTAAAAGGGAAAGAATTTTTCATGCCAATCAGAGTAGCAATAACAG
GAGAAGACCATGGACCTGAATTGGTGAAGATTATTCCTCTTTTGGGGAAGGATAAAGTGATAAATAGGCTGAAAAAAGCG
ATAAATTTAATAAAGTAA

Upstream 100 bases:

>100_bases
TTTTACACACAATTGTCACTTCATCTATTTAAAAAGCGCAAAATTTGTTATAATTAAATCTAGTATAAAGTATTAACTAA
AAAAGGATGGAGTGACACAC

Downstream 100 bases:

>100_bases
AAAGCGATGAGGAGAAGAGTATTATAACATAAGCCTTCAGAGAAGAGCTACCATAGGCTGCGAGTAGCTCGAGGATATGT
TATAAGAAGTGCATCTCCGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNNLRVRFAPSPTGAIHIGNIRTALFNYLFSRSEGATFVLRIEDTDLERSSKEFEELIFKELEWLGIEWDEGPDKPGPYG
PYRQSERLEIYHKFAQKLIEEKKAYRCYCTPEELEEDRRKAVERGDIPRYSGRCRYLTKEQEEAFIREGRKPVIRFIIPD
DEVIEFEDMIKGKITIKSDTLGGDMVIVKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HAPLILGPDRTKLSKRHGNTYIGQYRELGYLPEAMFNFLSLLSWSPEDNVEIMSKEEIIKKFNFRRIHSANPVFDIEKLN
WMNQQYIQKSSIERIVDLAIPHLRRAGYIDGIDDMVYNWLKDVISLYKDGLQYVAQITEKAKMFFVEEVEYSDETVKILN
SPNSKIVLEVVKKVIEEADEITEEYVKDLLKKLQKETKVKGKEFFMPIRVAITGEDHGPELVKIIPLLGKDKVINRLKKA
INLIK

Sequences:

>Translated_485_residues
MNNLRVRFAPSPTGAIHIGNIRTALFNYLFSRSEGATFVLRIEDTDLERSSKEFEELIFKELEWLGIEWDEGPDKPGPYG
PYRQSERLEIYHKFAQKLIEEKKAYRCYCTPEELEEDRRKAVERGDIPRYSGRCRYLTKEQEEAFIREGRKPVIRFIIPD
DEVIEFEDMIKGKITIKSDTLGGDMVIVKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HAPLILGPDRTKLSKRHGNTYIGQYRELGYLPEAMFNFLSLLSWSPEDNVEIMSKEEIIKKFNFRRIHSANPVFDIEKLN
WMNQQYIQKSSIERIVDLAIPHLRRAGYIDGIDDMVYNWLKDVISLYKDGLQYVAQITEKAKMFFVEEVEYSDETVKILN
SPNSKIVLEVVKKVIEEADEITEEYVKDLLKKLQKETKVKGKEFFMPIRVAITGEDHGPELVKIIPLLGKDKVINRLKKA
INLIK
>Mature_485_residues
MNNLRVRFAPSPTGAIHIGNIRTALFNYLFSRSEGATFVLRIEDTDLERSSKEFEELIFKELEWLGIEWDEGPDKPGPYG
PYRQSERLEIYHKFAQKLIEEKKAYRCYCTPEELEEDRRKAVERGDIPRYSGRCRYLTKEQEEAFIREGRKPVIRFIIPD
DEVIEFEDMIKGKITIKSDTLGGDMVIVKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HAPLILGPDRTKLSKRHGNTYIGQYRELGYLPEAMFNFLSLLSWSPEDNVEIMSKEEIIKKFNFRRIHSANPVFDIEKLN
WMNQQYIQKSSIERIVDLAIPHLRRAGYIDGIDDMVYNWLKDVISLYKDGLQYVAQITEKAKMFFVEEVEYSDETVKILN
SPNSKIVLEVVKKVIEEADEITEEYVKDLLKKLQKETKVKGKEFFMPIRVAITGEDHGPELVKIIPLLGKDKVINRLKKA
INLIK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=514, Percent_Identity=31.7120622568093, Blast_Score=280, Evalue=3e-75,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=39.0852390852391, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=34.4, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786895, Length=215, Percent_Identity=26.046511627907, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=32.4435318275154, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=32.7345309381238, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=224, Percent_Identity=25.4464285714286, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=332, Percent_Identity=24.6987951807229, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=34.8088531187123, Blast_Score=290, Evalue=1e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56472; Mature: 56472

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLRVRFAPSPTGAIHIGNIRTALFNYLFSRSEGATFVLRIEDTDLERSSKEFEELIFK
CCCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
ELEWLGIEWDEGPDKPGPYGPYRQSERLEIYHKFAQKLIEEKKAYRCYCTPEELEEDRRK
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
AVERGDIPRYSGRCRYLTKEQEEAFIREGRKPVIRFIIPDDEVIEFEDMIKGKITIKSDT
HHHHCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHCCEEEEEECC
LGGDMVIVKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHCCCCHHEEEEHHHCCCCCCCC
HAPLILGPDRTKLSKRHGNTYIGQYRELGYLPEAMFNFLSLLSWSPEDNVEIMSKEEIIK
CCCEEECCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHH
KFNFRRIHSANPVFDIEKLNWMNQQYIQKSSIERIVDLAIPHLRRAGYIDGIDDMVYNWL
HCCCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KDVISLYKDGLQYVAQITEKAKMFFVEEVEYSDETVKILNSPNSKIVLEVVKKVIEEADE
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ITEEYVKDLLKKLQKETKVKGKEFFMPIRVAITGEDHGPELVKIIPLLGKDKVINRLKKA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHEECCCCCHHHHHHHHHH
INLIK
HHHCC
>Mature Secondary Structure
MNNLRVRFAPSPTGAIHIGNIRTALFNYLFSRSEGATFVLRIEDTDLERSSKEFEELIFK
CCCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
ELEWLGIEWDEGPDKPGPYGPYRQSERLEIYHKFAQKLIEEKKAYRCYCTPEELEEDRRK
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
AVERGDIPRYSGRCRYLTKEQEEAFIREGRKPVIRFIIPDDEVIEFEDMIKGKITIKSDT
HHHHCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHCCEEEEEECC
LGGDMVIVKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHCCCCHHEEEEHHHCCCCCCCC
HAPLILGPDRTKLSKRHGNTYIGQYRELGYLPEAMFNFLSLLSWSPEDNVEIMSKEEIIK
CCCEEECCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHH
KFNFRRIHSANPVFDIEKLNWMNQQYIQKSSIERIVDLAIPHLRRAGYIDGIDDMVYNWL
HCCCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KDVISLYKDGLQYVAQITEKAKMFFVEEVEYSDETVKILNSPNSKIVLEVVKKVIEEADE
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ITEEYVKDLLKKLQKETKVKGKEFFMPIRVAITGEDHGPELVKIIPLLGKDKVINRLKKA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHEECCCCCHHHHHHHHHH
INLIK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA