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The map label for this gene is proS [H]

Identifier: 167039496

GI number: 167039496

Start: 879263

End: 880975

Strand: Direct

Name: proS [H]

Synonym: Teth514_0842

Alternate gene names: 167039496

Gene position: 879263-880975 (Clockwise)

Preceding gene: 167039495

Following gene: 167039497

Centisome position: 35.78

GC content: 36.08

Gene sequence:

>1713_bases
ATGAGGTTATCGCAATTATTAGTTCCGACTTTAAGAGAAATTCCTGCTGAAGCTGAAATTCCCAGCCATATTTTAATGTT
AAAGGCTGCATTGATGAGGAAATTGGCTTCAGGGGTATATATTTACTTGCCATTAGGACAAAGGGTACTTAGAAAAGTGG
AGCAAATTGTAAGAGAAGAGATGGACAGAGCTGGCAGTCAAGAGGTACTAATGTCTGCTCTTATTCCAGCAGAACTTTTG
AAAGAAAGTGGAAGATGGGATGTATTTGGCCCTGAGATGTTTAAGCTAAAAGATAGAAATGAAAGGGACTTTTGCTTAGG
GCCTACACACGAAGAGGTTTTTACAGACCTTATTAGAAATGAAGTGAAGTCTTATAGGCAGCTTCCTCTTATTTTATATC
AAATTCAAACTAAGTTTAGAGATGAAAGGCGCCCGCGATTTGGCGTCATGAGAAGTAGAGAATTTATAATGAAGGACGCT
TACAGCTTTGATGTGGATTGGGAAGGTTTGGACAAGTCCTTTAATAAAATGTATGAGGCTTACTGCCGAATATTTGATAG
ATGTGGACTTAAATATTTGGTGGTAGAAGCTGATTCAGGTGCTATGGGAGGAAAAGACTCAAAAGAGTTTATGGTAATTT
CTAGTATAGGGGAAGCAGTAATAGCTTACTGTGACAATTGTGGTTATGCTGCAAATGAGGAAAAGGCAGAATGCCTTATC
AATCAAGAAATAGTTGAAGAGATGTTGCCAAAAGAAGAAGTATATACACCAAATGTTAGAACAATTGAAGAGTTGGTTAA
TTTTTTGGGAATTACTCCTAATAAATTTGTAAAAACTCTTATATACAAGGCAAAGGACAACGTTGTAGCAGCATTAGTTC
GAGGAGATAGAGACTTAAACGAGACAAAGCTTTTAAATATTTTAGGAATAAGAGAAGAGGAGTTAGAATTGGCAGATGCA
TCAATAGTGGAAAAGGTTACAGGAGCAAAAGTGGGATTTGCAGGACCTATAGGATTAAAAGGAGAAGTAATGATAATAGT
AGATAATGAGATTCCGCAAATGAGGAATTTTATAGTAGGAGCAAACGAGACAGATTATCATATTAAAAATGTCAACTATG
GTAGAGACTTTAAAGCTGATGTTGTGGCGGATATCAAAAATGTTATTGAAGGAGATAAGTGTCCAAGGTGTGGTTCACCG
CTTAAAATTGACAGAGGTATAGAAGTAGGACATATTTTTAAGTTAGGGACAAAGTACAGTGATGCATTGGGAGCTAAGTA
TGTAGATGAAGAGGGGAATGAAAAGCCAATTATAATGGGCTGCTATGGAATAGGAATTAATAGAACGGTGGCGGCTATTA
TAGAGCAACACCATGATGAGAAAGGTATTATATGGCCTATGAGTGTTGCACCCTATCATGTGATTATAGTGCCTGTCAAT
GTTTCTAATGAAGCTCAAAATCGAGTAGCAGAAGATATATATGCTGCTTTGCAAAAAGAGGGAATAGAGGTTTTAATAGA
TGATAGAGATTTAAGAGCTGGTGTCAAATTTAATGACGCCGATTTGTTAGGAATACCTATAAGGATTACAGTTGGGAAAA
AAGTAGATGATGGAATAGTGGAAATAAAATTGAGAGAAAATGAAGAAGCAGAAGAGGTAAAAATTTCTGATGTTGTAGAA
AAGGTGAAAAATATTATAAAAGAGAAAATGTAA

Upstream 100 bases:

>100_bases
TAAAAGCCTATGTTATTGTCAGTTTAGAAGAATTAGGTGTTGACATAAGAAAGTAGGTAAAATAAAATTTACTTAAAATT
ATGCACTTAGGAGGATGGAG

Downstream 100 bases:

>100_bases
GGGATTTCTTAAAAGGAATCCCTTATTTTGCATAAAGAAAGTTTGTATGTGAAAAAATATTTGTCAAAAGGTTGATAAAA
AGGAGGGACTCTTGTGAATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRLSQLLVPTLREIPAEAEIPSHILMLKAALMRKLASGVYIYLPLGQRVLRKVEQIVREEMDRAGSQEVLMSALIPAELL
KESGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLIRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDVDWEGLDKSFNKMYEAYCRIFDRCGLKYLVVEADSGAMGGKDSKEFMVISSIGEAVIAYCDNCGYAANEEKAECLI
NQEIVEEMLPKEEVYTPNVRTIEELVNFLGITPNKFVKTLIYKAKDNVVAALVRGDRDLNETKLLNILGIREEELELADA
SIVEKVTGAKVGFAGPIGLKGEVMIIVDNEIPQMRNFIVGANETDYHIKNVNYGRDFKADVVADIKNVIEGDKCPRCGSP
LKIDRGIEVGHIFKLGTKYSDALGAKYVDEEGNEKPIIMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIIVPVN
VSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIVEIKLRENEEAEEVKISDVVE
KVKNIIKEKM

Sequences:

>Translated_570_residues
MRLSQLLVPTLREIPAEAEIPSHILMLKAALMRKLASGVYIYLPLGQRVLRKVEQIVREEMDRAGSQEVLMSALIPAELL
KESGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLIRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDVDWEGLDKSFNKMYEAYCRIFDRCGLKYLVVEADSGAMGGKDSKEFMVISSIGEAVIAYCDNCGYAANEEKAECLI
NQEIVEEMLPKEEVYTPNVRTIEELVNFLGITPNKFVKTLIYKAKDNVVAALVRGDRDLNETKLLNILGIREEELELADA
SIVEKVTGAKVGFAGPIGLKGEVMIIVDNEIPQMRNFIVGANETDYHIKNVNYGRDFKADVVADIKNVIEGDKCPRCGSP
LKIDRGIEVGHIFKLGTKYSDALGAKYVDEEGNEKPIIMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIIVPVN
VSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIVEIKLRENEEAEEVKISDVVE
KVKNIIKEKM
>Mature_570_residues
MRLSQLLVPTLREIPAEAEIPSHILMLKAALMRKLASGVYIYLPLGQRVLRKVEQIVREEMDRAGSQEVLMSALIPAELL
KESGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLIRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDVDWEGLDKSFNKMYEAYCRIFDRCGLKYLVVEADSGAMGGKDSKEFMVISSIGEAVIAYCDNCGYAANEEKAECLI
NQEIVEEMLPKEEVYTPNVRTIEELVNFLGITPNKFVKTLIYKAKDNVVAALVRGDRDLNETKLLNILGIREEELELADA
SIVEKVTGAKVGFAGPIGLKGEVMIIVDNEIPQMRNFIVGANETDYHIKNVNYGRDFKADVVADIKNVIEGDKCPRCGSP
LKIDRGIEVGHIFKLGTKYSDALGAKYVDEEGNEKPIIMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIIVPVN
VSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIVEIKLRENEEAEEVKISDVVE
KVKNIIKEKM

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=214, Percent_Identity=44.392523364486, Blast_Score=206, Evalue=6e-53,
Organism=Escherichia coli, GI1786392, Length=577, Percent_Identity=48.7001733102253, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=39.5238095238095, Blast_Score=165, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71991342, Length=175, Percent_Identity=30.2857142857143, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=542, Percent_Identity=32.1033210332103, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24656200, Length=214, Percent_Identity=38.785046728972, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64398; Mature: 64398

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQLLVPTLREIPAEAEIPSHILMLKAALMRKLASGVYIYLPLGQRVLRKVEQIVREE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
MDRAGSQEVLMSALIPAELLKESGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLIRN
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
EVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDAYSFDVDWEGLDKSFNKMYEA
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YCRIFDRCGLKYLVVEADSGAMGGKDSKEFMVISSIGEAVIAYCDNCGYAANEEKAECLI
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHH
NQEIVEEMLPKEEVYTPNVRTIEELVNFLGITPNKFVKTLIYKAKDNVVAALVRGDRDLN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
ETKLLNILGIREEELELADASIVEKVTGAKVGFAGPIGLKGEVMIIVDNEIPQMRNFIVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHEEEC
ANETDYHIKNVNYGRDFKADVVADIKNVIEGDKCPRCGSPLKIDRGIEVGHIFKLGTKYS
CCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEHEEEECCCHH
DALGAKYVDEEGNEKPIIMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIIVPVN
HHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEE
VSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIV
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEEEEEEEECCCCCCCEE
EIKLRENEEAEEVKISDVVEKVKNIIKEKM
EEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLSQLLVPTLREIPAEAEIPSHILMLKAALMRKLASGVYIYLPLGQRVLRKVEQIVREE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
MDRAGSQEVLMSALIPAELLKESGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLIRN
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
EVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDAYSFDVDWEGLDKSFNKMYEA
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YCRIFDRCGLKYLVVEADSGAMGGKDSKEFMVISSIGEAVIAYCDNCGYAANEEKAECLI
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHH
NQEIVEEMLPKEEVYTPNVRTIEELVNFLGITPNKFVKTLIYKAKDNVVAALVRGDRDLN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
ETKLLNILGIREEELELADASIVEKVTGAKVGFAGPIGLKGEVMIIVDNEIPQMRNFIVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHEEEC
ANETDYHIKNVNYGRDFKADVVADIKNVIEGDKCPRCGSPLKIDRGIEVGHIFKLGTKYS
CCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEHEEEECCCHH
DALGAKYVDEEGNEKPIIMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIIVPVN
HHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEE
VSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIV
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEEEEEEEECCCCCCCEE
EIKLRENEEAEEVKISDVVEKVKNIIKEKM
EEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA