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The map label for this gene is ispF [H]

Identifier: 167039495

GI number: 167039495

Start: 878721

End: 879218

Strand: Direct

Name: ispF [H]

Synonym: Teth514_0841

Alternate gene names: 167039495

Gene position: 878721-879218 (Clockwise)

Preceding gene: 167039494

Following gene: 167039496

Centisome position: 35.76

GC content: 33.53

Gene sequence:

>498_bases
ATGATTGAAGTGAAAGTAGGGTTAGGATATGACGTCCACAAGTTTGAAAAAGGAAGAAAGTTAATACTGGGAGGAGTAGA
AATTCCCTATGATAAAGGTTTGGCAGGCCATTCTGATGCAGATGTTTTGATACACGCCTTAATTGATGCTATTTTAGGAG
CAGCTGGTTTAGGGGATATAGGGGAGCACTTTCCTGATACGGAGATAGCCTATAAAGATATTGATAGTAGCCTTTTATTA
AAAAAAGTGTTGGCAATAATAGAGAATAAATTCAGAGTAAATAATGTGGATTGTATTATTGTAGCGCAAAGGCCTAAGTT
ATCTCCTTATAAAGAGCAAATAAGAATAAAATTATCAGAATTATTAAAAATTGAGAAAGAAAGAATAAATGTAAAAGCAA
AAACAGAGGAAGGATTAGGTTTTACAGGTAGACAAGAGGGAATAAAAGCCTATGTTATTGTCAGTTTAGAAGAATTAGGT
GTTGACATAAGAAAGTAG

Upstream 100 bases:

>100_bases
AATAAAGGGAATTTTGTAAAAAAGATTAAATCATCTCGCTTTGCAAGAGGTGTAAGCATGAGTTTAGAAGTTGATAAAGA
AGGGAATTTTAGCGTAAAGG

Downstream 100 bases:

>100_bases
GTAAAATAAAATTTACTTAAAATTATGCACTTAGGAGGATGGAGATGAGGTTATCGCAATTATTAGTTCCGACTTTAAGA
GAAATTCCTGCTGAAGCTGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIEVKVGLGYDVHKFEKGRKLILGGVEIPYDKGLAGHSDADVLIHALIDAILGAAGLGDIGEHFPDTEIAYKDIDSSLLL
KKVLAIIENKFRVNNVDCIIVAQRPKLSPYKEQIRIKLSELLKIEKERINVKAKTEEGLGFTGRQEGIKAYVIVSLEELG
VDIRK

Sequences:

>Translated_165_residues
MIEVKVGLGYDVHKFEKGRKLILGGVEIPYDKGLAGHSDADVLIHALIDAILGAAGLGDIGEHFPDTEIAYKDIDSSLLL
KKVLAIIENKFRVNNVDCIIVAQRPKLSPYKEQIRIKLSELLKIEKERINVKAKTEEGLGFTGRQEGIKAYVIVSLEELG
VDIRK
>Mature_165_residues
MIEVKVGLGYDVHKFEKGRKLILGGVEIPYDKGLAGHSDADVLIHALIDAILGAAGLGDIGEHFPDTEIAYKDIDSSLLL
KKVLAIIENKFRVNNVDCIIVAQRPKLSPYKEQIRIKLSELLKIEKERINVKAKTEEGLGFTGRQEGIKAYVIVSLEELG
VDIRK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=49.3506493506493, Blast_Score=141, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 18261; Mature: 18261

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVKVGLGYDVHKFEKGRKLILGGVEIPYDKGLAGHSDADVLIHALIDAILGAAGLGDI
CEEEEECCCCCHHHHCCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
GEHFPDTEIAYKDIDSSLLLKKVLAIIENKFRVNNVDCIIVAQRPKLSPYKEQIRIKLSE
HHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHH
LLKIEKERINVKAKTEEGLGFTGRQEGIKAYVIVSLEELGVDIRK
HHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCCCCCC
>Mature Secondary Structure
MIEVKVGLGYDVHKFEKGRKLILGGVEIPYDKGLAGHSDADVLIHALIDAILGAAGLGDI
CEEEEECCCCCHHHHCCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
GEHFPDTEIAYKDIDSSLLLKKVLAIIENKFRVNNVDCIIVAQRPKLSPYKEQIRIKLSE
HHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHH
LLKIEKERINVKAKTEEGLGFTGRQEGIKAYVIVSLEELGVDIRK
HHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]