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The map label for this gene is tilS [H]

Identifier: 167039445

GI number: 167039445

Start: 823582

End: 824958

Strand: Direct

Name: tilS [H]

Synonym: Teth514_0791

Alternate gene names: 167039445

Gene position: 823582-824958 (Clockwise)

Preceding gene: 167039444

Following gene: 167039446

Centisome position: 33.52

GC content: 30.21

Gene sequence:

>1377_bases
ATGATTGATAAGGTTGTTTCCACAATTAAAAGGTATAAAATGATTGAGACAAATGATAAAATTGTAATGGGGGTTTCCGG
TGGACCTGATTCTTTGTGTATGCTGGATATTTTATACAATTTAAAAGATTTATTTAATCTTAAGTTGTATATAGTACATG
TTAATCATATGATAAGAGGAGAAGCAGCTAAAAGGGATGCGCAATTTGTTGAAGATATGTGTCGCAAATTGAAACTCCCA
TTTTTTTTATTTGAAAAAGATGTAAAAAAAATAGCCAAAGAAATGGGATATTCAGAAGAAGAGGCTGGTCGATATGTGAG
GTATCAGGCTTTTGAGGAAGTATTAAAAGAAGTTAAGGGTAATAAAATAGCAGTAGCTCATAATAAAAATGATGTAGTGG
AAACAGTTTTTTTAAATTTAATAAGAGGTTCTGGAATGGCTGGCCTCATTGGAATAAAACCGGTAAATGGAAATATAATA
AGGCCTCTCATTGAAATAGAAAGGGAAGAAATAGAGAAATACCTTAAGGAAAAAGATTTAAAGCCAGCAATAGATTTTAC
AAATTATGAGGATTTATATAAGAGAAATAAAGTAAGACTTAAATTAATTCCCTTTATAAATGAAACTTTTGAAGTAGATA
TAATAGAAAATATCTACAGGATGGCAAAAATTATTTTAGAGGAGAATGACTATTTAGAAAAAGAGTGTGAAAAGATTTTT
AATGAGGTTTGCTATTTTAATCAAGAGGAAATTTTTGCTGATATAAAAAAAATGAAGACACAGCATCCCGCTATTCAGCG
GCGCTTAGTTAGGCTTATGTACCAAAAATTAAAAGGAGATATTTATGGGTTAGAATACATCCATGTAGAGGATGTTTTAA
GCCTTTTAGACAAACCAACTTCCTCAAAAATTGATTTACCCTTTGAAATTGAAGCTTTAAAAAGCTATAATAATCTTATA
ATGAGAAAAGTAAAAGAAAAAGAGGTCAAGACTTTTTGGGCAAAATTAAATATTCCTGGAATTACTAAGATATGTGGTAT
TGGTCAATTTAAAACTGCTGTTTTAGCTATTGATGAGGTAAAAAAACTCAACATAGGAAAATACACGAAATTTTTTGACT
ATGATAAAATACAGGGAGATGTGGTAGTTAGAAATAGGCAAGCAGGGGATATTTTTTCTCCTCTTAACTTGGGGGGGAGT
AAAAAATTAAAAGAATTTTTTATTGACGAGAAAATACCCCGCGAAATAAGAGATAGCATTCCTTTATTAGCCATAGACAA
TGAAATTGTGTGGGTTGTAGGTTATAGAATGAGTGATAAATTTAAAGTAGATGAAAATACAAGAAAAGTCCTTGTGATAG
AGTATACCAAAGGATAA

Upstream 100 bases:

>100_bases
GAATACTTTTGTGGAGGGGTTTTTTAGGTTTGATTTAGAAAGGAGGATATTATATAATCATTATATAACATGAGTATATA
TCGTTATAAGGGGTTAAGAA

Downstream 100 bases:

>100_bases
AATAAAAAAAGAGGAGGTTAAATTTATGTCGAGTCCAACACAAGATATACAGGAGATTTTGATTACGGAAGAGCAATTAA
AAGAAAAAGTGAAAGAGTTA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MIDKVVSTIKRYKMIETNDKIVMGVSGGPDSLCMLDILYNLKDLFNLKLYIVHVNHMIRGEAAKRDAQFVEDMCRKLKLP
FFLFEKDVKKIAKEMGYSEEEAGRYVRYQAFEEVLKEVKGNKIAVAHNKNDVVETVFLNLIRGSGMAGLIGIKPVNGNII
RPLIEIEREEIEKYLKEKDLKPAIDFTNYEDLYKRNKVRLKLIPFINETFEVDIIENIYRMAKIILEENDYLEKECEKIF
NEVCYFNQEEIFADIKKMKTQHPAIQRRLVRLMYQKLKGDIYGLEYIHVEDVLSLLDKPTSSKIDLPFEIEALKSYNNLI
MRKVKEKEVKTFWAKLNIPGITKICGIGQFKTAVLAIDEVKKLNIGKYTKFFDYDKIQGDVVVRNRQAGDIFSPLNLGGS
KKLKEFFIDEKIPREIRDSIPLLAIDNEIVWVVGYRMSDKFKVDENTRKVLVIEYTKG

Sequences:

>Translated_458_residues
MIDKVVSTIKRYKMIETNDKIVMGVSGGPDSLCMLDILYNLKDLFNLKLYIVHVNHMIRGEAAKRDAQFVEDMCRKLKLP
FFLFEKDVKKIAKEMGYSEEEAGRYVRYQAFEEVLKEVKGNKIAVAHNKNDVVETVFLNLIRGSGMAGLIGIKPVNGNII
RPLIEIEREEIEKYLKEKDLKPAIDFTNYEDLYKRNKVRLKLIPFINETFEVDIIENIYRMAKIILEENDYLEKECEKIF
NEVCYFNQEEIFADIKKMKTQHPAIQRRLVRLMYQKLKGDIYGLEYIHVEDVLSLLDKPTSSKIDLPFEIEALKSYNNLI
MRKVKEKEVKTFWAKLNIPGITKICGIGQFKTAVLAIDEVKKLNIGKYTKFFDYDKIQGDVVVRNRQAGDIFSPLNLGGS
KKLKEFFIDEKIPREIRDSIPLLAIDNEIVWVVGYRMSDKFKVDENTRKVLVIEYTKG
>Mature_458_residues
MIDKVVSTIKRYKMIETNDKIVMGVSGGPDSLCMLDILYNLKDLFNLKLYIVHVNHMIRGEAAKRDAQFVEDMCRKLKLP
FFLFEKDVKKIAKEMGYSEEEAGRYVRYQAFEEVLKEVKGNKIAVAHNKNDVVETVFLNLIRGSGMAGLIGIKPVNGNII
RPLIEIEREEIEKYLKEKDLKPAIDFTNYEDLYKRNKVRLKLIPFINETFEVDIIENIYRMAKIILEENDYLEKECEKIF
NEVCYFNQEEIFADIKKMKTQHPAIQRRLVRLMYQKLKGDIYGLEYIHVEDVLSLLDKPTSSKIDLPFEIEALKSYNNLI
MRKVKEKEVKTFWAKLNIPGITKICGIGQFKTAVLAIDEVKKLNIGKYTKFFDYDKIQGDVVVRNRQAGDIFSPLNLGGS
KKLKEFFIDEKIPREIRDSIPLLAIDNEIVWVVGYRMSDKFKVDENTRKVLVIEYTKG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=430, Percent_Identity=25.5813953488372, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787606, Length=180, Percent_Identity=27.2222222222222, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53391; Mature: 53391

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKVVSTIKRYKMIETNDKIVMGVSGGPDSLCMLDILYNLKDLFNLKLYIVHVNHMIRG
CHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECHHHHC
EAAKRDAQFVEDMCRKLKLPFFLFEKDVKKIAKEMGYSEEEAGRYVRYQAFEEVLKEVKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCC
NKIAVAHNKNDVVETVFLNLIRGSGMAGLIGIKPVNGNIIRPLIEIEREEIEKYLKEKDL
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
KPAIDFTNYEDLYKRNKVRLKLIPFINETFEVDIIENIYRMAKIILEENDYLEKECEKIF
CCCCCCCCHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NEVCYFNQEEIFADIKKMKTQHPAIQRRLVRLMYQKLKGDIYGLEYIHVEDVLSLLDKPT
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCC
SSKIDLPFEIEALKSYNNLIMRKVKEKEVKTFWAKLNIPGITKICGIGQFKTAVLAIDEV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHH
KKLNIGKYTKFFDYDKIQGDVVVRNRQAGDIFSPLNLGGSKKLKEFFIDEKIPREIRDSI
HHCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCC
PLLAIDNEIVWVVGYRMSDKFKVDENTRKVLVIEYTKG
CEEEECCEEEEEEEEEECCCEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MIDKVVSTIKRYKMIETNDKIVMGVSGGPDSLCMLDILYNLKDLFNLKLYIVHVNHMIRG
CHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECHHHHC
EAAKRDAQFVEDMCRKLKLPFFLFEKDVKKIAKEMGYSEEEAGRYVRYQAFEEVLKEVKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCC
NKIAVAHNKNDVVETVFLNLIRGSGMAGLIGIKPVNGNIIRPLIEIEREEIEKYLKEKDL
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
KPAIDFTNYEDLYKRNKVRLKLIPFINETFEVDIIENIYRMAKIILEENDYLEKECEKIF
CCCCCCCCHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NEVCYFNQEEIFADIKKMKTQHPAIQRRLVRLMYQKLKGDIYGLEYIHVEDVLSLLDKPT
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCC
SSKIDLPFEIEALKSYNNLIMRKVKEKEVKTFWAKLNIPGITKICGIGQFKTAVLAIDEV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHH
KKLNIGKYTKFFDYDKIQGDVVVRNRQAGDIFSPLNLGGSKKLKEFFIDEKIPREIRDSI
HHCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCC
PLLAIDNEIVWVVGYRMSDKFKVDENTRKVLVIEYTKG
CEEEECCEEEEEEEEEECCCEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]