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The map label for this gene is cisA [H]

Identifier: 167039356

GI number: 167039356

Start: 729848

End: 731368

Strand: Reverse

Name: cisA [H]

Synonym: Teth514_0698

Alternate gene names: 167039356

Gene position: 731368-729848 (Counterclockwise)

Preceding gene: 167039357

Following gene: 167039351

Centisome position: 29.76

GC content: 34.98

Gene sequence:

>1521_bases
ATGTCAAGTGCAGAAATAAGAGCTGCTGCATATGCAAGATACTCCAGCGACAATCAAAGGGAGGAAAGTATTGAAGCTCA
ATTAAGAGCTATAAAAGAGTACTGCGAAAAGAATAATATCCAGCTAGTAAAGATATACACTGATGAAGCAAAAAGTGCAA
CAACTGATGATAGGCCGGCATTCCTGCAGATGATAAAGGACAGCTCACTGGGGCTGTTTAACGCTGTTATTGTTCATAAA
CTGGACAGGTTTGCACGCAACAGATATGACAGCGCTTTCTACAGGAGACAGTTAAAAAAGAACGGCGTAAAACTCATTTC
AGTACTTGAACAGTTAGACGATTCACCAGAAAGCATTATATTGGAAAGTGTTTTAGAAGGAATGGCCGAATATTATTCTA
AAAACCTCGCAAGGGAGGTAATGAAGGGATTGAAAGAAAATGCTTTTCAGGCCAAATTTAATGGAGGTATACCACCTTTA
GGATATGATATTGTAGATGAAAAATATGTGATAAACGAAAAAGAAGCTGAGGCAGTAAGATTGATTTTTGAATTATATGC
ACAGGGGTATGGATATAGACACATAATTGATGAATTAAATTTAAGAGGCTACAGAACTAAGAAAGGAAATCCATTCAGCA
AAAATAGCATTCACGAAATTTTGAAAAATGAGAAATACATTGGTAAATACATATTTAACAAAGGCACAAAGAAAAACCAT
AGAATGGTAAAAGAAGACGTAATCGTTATAGATAATGCTATTCCTGCAATAATAGATAAAGACCTGTTCGAACAGGTACA
AAAGAAAATAGAAGAAAGAAAAATAACAAGAGGAGCTAATTCTGCAAAAAGGGTTTATCTTTTGTCAGGTCTTGTTTACT
GCGGAATATGCGATTCTAAAATGGTAGGGTGTTTAAGCAGAGGGAAATATGTAACTTATAGATGCAGCAGAAAGGACAGA
ACAAAGCAGTGCACAAACAGAGATATAAACAAAGAAAAACTGGAAGCTTTTGTCATAGATGAGCTGAACAAGAGAGTACT
TGACCCAAAAGTAGCACCAGTATTAGCTAAACTTTTAAATGAAGAAAATCAGAAATTAAATAAAGAAAGAGTAGAAGAAA
TTACTCTTTTAAAAAAGCGCTATAAGGAGGTTGAAGAGCAGATGAAAAACATAGTAGAAGCTATTGCTCAAGGTATGTTC
CAGCCGATTATGAAGGAAAAAATGAATGAATTAGAAGCAATGAGAAATGAACTCGCTTACAGAATTAAAGAGCTGGAAAA
TAAAAAAGAAACAAGCTTTATTACACCAGAAATGATTTTGAAAATCTTTGAAAAGGATAGGGAAATAATTAATTCATCGA
AGGATCCTCATGAAATTAAAGAAGTGCTTAAAAACTACATAAAAAAAATAATCGTCACCCCAGATGAAATAAAGTGTGAA
TTCTACTTCTGGATAGGTGACGATTTCCGTTCTTTGCTGGTAGCGGCGAGTGGATTTGAACCACCGACACTGCGGGTATG
A

Upstream 100 bases:

>100_bases
AAGAAGAAGAATTTAACGACAATGAATTTGATGATTGGTAATAAGAGGTGTTGAAAGCAATCTTACAGCAAACTCAAAAT
AAATCTAGAGGAGGAGTTAT

Downstream 100 bases:

>100_bases
ACCGCATGCTCTGGCCAACTGAGCTACGCCGCCATATTTTTGGTTGCGGGGGTAGGATTTGAACCTACGACCTCCGGGTT
ATGAGCCCGACGAGCTACCG

Product: recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSSAEIRAAAYARYSSDNQREESIEAQLRAIKEYCEKNNIQLVKIYTDEAKSATTDDRPAFLQMIKDSSLGLFNAVIVHK
LDRFARNRYDSAFYRRQLKKNGVKLISVLEQLDDSPESIILESVLEGMAEYYSKNLAREVMKGLKENAFQAKFNGGIPPL
GYDIVDEKYVINEKEAEAVRLIFELYAQGYGYRHIIDELNLRGYRTKKGNPFSKNSIHEILKNEKYIGKYIFNKGTKKNH
RMVKEDVIVIDNAIPAIIDKDLFEQVQKKIEERKITRGANSAKRVYLLSGLVYCGICDSKMVGCLSRGKYVTYRCSRKDR
TKQCTNRDINKEKLEAFVIDELNKRVLDPKVAPVLAKLLNEENQKLNKERVEEITLLKKRYKEVEEQMKNIVEAIAQGMF
QPIMKEKMNELEAMRNELAYRIKELENKKETSFITPEMILKIFEKDREIINSSKDPHEIKEVLKNYIKKIIVTPDEIKCE
FYFWIGDDFRSLLVAASGFEPPTLRV

Sequences:

>Translated_506_residues
MSSAEIRAAAYARYSSDNQREESIEAQLRAIKEYCEKNNIQLVKIYTDEAKSATTDDRPAFLQMIKDSSLGLFNAVIVHK
LDRFARNRYDSAFYRRQLKKNGVKLISVLEQLDDSPESIILESVLEGMAEYYSKNLAREVMKGLKENAFQAKFNGGIPPL
GYDIVDEKYVINEKEAEAVRLIFELYAQGYGYRHIIDELNLRGYRTKKGNPFSKNSIHEILKNEKYIGKYIFNKGTKKNH
RMVKEDVIVIDNAIPAIIDKDLFEQVQKKIEERKITRGANSAKRVYLLSGLVYCGICDSKMVGCLSRGKYVTYRCSRKDR
TKQCTNRDINKEKLEAFVIDELNKRVLDPKVAPVLAKLLNEENQKLNKERVEEITLLKKRYKEVEEQMKNIVEAIAQGMF
QPIMKEKMNELEAMRNELAYRIKELENKKETSFITPEMILKIFEKDREIINSSKDPHEIKEVLKNYIKKIIVTPDEIKCE
FYFWIGDDFRSLLVAASGFEPPTLRV
>Mature_505_residues
SSAEIRAAAYARYSSDNQREESIEAQLRAIKEYCEKNNIQLVKIYTDEAKSATTDDRPAFLQMIKDSSLGLFNAVIVHKL
DRFARNRYDSAFYRRQLKKNGVKLISVLEQLDDSPESIILESVLEGMAEYYSKNLAREVMKGLKENAFQAKFNGGIPPLG
YDIVDEKYVINEKEAEAVRLIFELYAQGYGYRHIIDELNLRGYRTKKGNPFSKNSIHEILKNEKYIGKYIFNKGTKKNHR
MVKEDVIVIDNAIPAIIDKDLFEQVQKKIEERKITRGANSAKRVYLLSGLVYCGICDSKMVGCLSRGKYVTYRCSRKDRT
KQCTNRDINKEKLEAFVIDELNKRVLDPKVAPVLAKLLNEENQKLNKERVEEITLLKKRYKEVEEQMKNIVEAIAQGMFQ
PIMKEKMNELEAMRNELAYRIKELENKKETSFITPEMILKIFEKDREIINSSKDPHEIKEVLKNYIKKIIVTPDEIKCEF
YFWIGDDFRSLLVAASGFEPPTLRV

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786756, Length=513, Percent_Identity=23.1968810916179, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 58781; Mature: 58649

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAEIRAAAYARYSSDNQREESIEAQLRAIKEYCEKNNIQLVKIYTDEAKSATTDDRPA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHH
FLQMIKDSSLGLFNAVIVHKLDRFARNRYDSAFYRRQLKKNGVKLISVLEQLDDSPESII
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHH
LESVLEGMAEYYSKNLAREVMKGLKENAFQAKFNGGIPPLGYDIVDEKYVINEKEAEAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCCCCCCCCHHHHHHH
LIFELYAQGYGYRHIIDELNLRGYRTKKGNPFSKNSIHEILKNEKYIGKYIFNKGTKKNH
HHHHHHHCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHH
RMVKEDVIVIDNAIPAIIDKDLFEQVQKKIEERKITRGANSAKRVYLLSGLVYCGICDSK
HHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
MVGCLSRGKYVTYRCSRKDRTKQCTNRDINKEKLEAFVIDELNKRVLDPKVAPVLAKLLN
HHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EENQKLNKERVEEITLLKKRYKEVEEQMKNIVEAIAQGMFQPIMKEKMNELEAMRNELAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKELENKKETSFITPEMILKIFEKDREIINSSKDPHEIKEVLKNYIKKIIVTPDEIKCE
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
FYFWIGDDFRSLLVAASGFEPPTLRV
EEEEECHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSAEIRAAAYARYSSDNQREESIEAQLRAIKEYCEKNNIQLVKIYTDEAKSATTDDRPA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHH
FLQMIKDSSLGLFNAVIVHKLDRFARNRYDSAFYRRQLKKNGVKLISVLEQLDDSPESII
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHH
LESVLEGMAEYYSKNLAREVMKGLKENAFQAKFNGGIPPLGYDIVDEKYVINEKEAEAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCCCCCCCCHHHHHHH
LIFELYAQGYGYRHIIDELNLRGYRTKKGNPFSKNSIHEILKNEKYIGKYIFNKGTKKNH
HHHHHHHCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHH
RMVKEDVIVIDNAIPAIIDKDLFEQVQKKIEERKITRGANSAKRVYLLSGLVYCGICDSK
HHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
MVGCLSRGKYVTYRCSRKDRTKQCTNRDINKEKLEAFVIDELNKRVLDPKVAPVLAKLLN
HHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EENQKLNKERVEEITLLKKRYKEVEEQMKNIVEAIAQGMFQPIMKEKMNELEAMRNELAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKELENKKETSFITPEMILKIFEKDREIINSSKDPHEIKEVLKNYIKKIIVTPDEIKCE
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
FYFWIGDDFRSLLVAASGFEPPTLRV
EEEEECHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]