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The map label for this gene is aegA [H]

Identifier: 167039310

GI number: 167039310

Start: 677720

End: 679120

Strand: Reverse

Name: aegA [H]

Synonym: Teth514_0651

Alternate gene names: 167039310

Gene position: 679120-677720 (Counterclockwise)

Preceding gene: 167039311

Following gene: 167039302

Centisome position: 27.64

GC content: 45.4

Gene sequence:

>1401_bases
ATGCCGTTAAATAGAAGTAAAAAGAAAACACCCATGCCTACCCAAAAGCCTGACGTCCGCAGGCGTAATTTTAGCGAAGT
AGCTTTGGGCTATTCGGAAGAGGAGGCAGTAAGTGAAGCCCAGAGGTGCCTTCAGTGTAAAAAACCAGGATGTGTTGAAG
GGTGCCCCGTTCAGGTACAAATTCCCCAGTTTATAAAGCGTATTGCAGAAAGAGATTTTGAAGGAGCTATAAAGATTATA
AAGGAAACCAACAGCTTGCCCGCCATATGTGGCAGAGTCTGTCCACAAGAAGAGCAATGCGAGAAAAACTGTGTTTTGGG
CAAAGTAGGTGAACCTGTAGCAATAGGTCGCCTAGAACGATTTGCAGCAGACTGGGAAAGAGCCAAAGGCATCCGTCCCT
CGGTAATTCCCAAAAAGCTTGGTAAAAAAGTGGCTATCATCGGTTCCGGGCCCGCCGGTCTCACCTGTGCAGGAGATTTA
GCTAAACTCGGCTACGATGTAACGATCTTTGAAGCTCTTCACAAACCCGGCGGCGTATTGATCTACGGTATTCCTGAATT
CAGACTACCGAAAATTATAGTGGAACAAGAAGTAGAATTTATACAACAGCTAGGCGTAGAAATTAAAACTAACATGGTTA
TGGGTAAAGTTTTAACGATAGACGACCTTTTTGAAATGGGGTATGAGGCTGTCTTTACCGGCACTGGAGCTGGTCTCCCG
AAATTTATGGGCATACCTGGTGAAAACTACCTGGGCGTGTATTCAGCCAACGAGTTTCTAACCAGGATAAACCTCATGAA
AGCCTATTCTTTTCCCAACACTGACACCCCCATAAAGGTAGGTAAAAAAGTAGCAGTAATAGGGGGTGGCAATGTAGCCA
TGGATGCAGCCAGGTCTGCAGTAAGAATGGGAGCTGAAGAGGTTCATATTGTGTATCGTCGTTCCGAGGAAGAAATGCCC
GCAAGAAAAGAAGAAGTTGAGAACGCTAAAGAGGAAGGTATTATATTCGATTTGCTTACAAATCCAGCGAAAATAATAGG
CAACGAAAATGGTTGGGTCAAAGGTATTGAGTGTATCCGCATGGAACTTGGAGAACCTGACGCTTCGGGTCGTCGCCGTC
CAGTTCCTATAATGGGTTCTGAACTTGTTATGGATATCGATACGGTAGTAATCGCCATAGGTACTGGCCCTAATCCCCTC
CTCACAAAGGCCACTGAAGGCTTAGAACTTACAAAACATGGTTATATCGCTGTAGATGAGGAGGGACGGACTTCCAGAGA
AGGGGTATGGGCTGGAGGCGATATTGTAACAGGTTCAGCTACTGTGATTTTGGCCATGGGTGCCGGAAAAAAAGCAGCTC
GTTCCATCCATGAATATCTACAAAACAAAAAATGGCGGTAA

Upstream 100 bases:

>100_bases
AGATTGGCCACTTATAAAGAGGAAGAAAAAATTTCCCTCGAAAGGTTTAATAAAGCTCATGAATGCAAATTAATCAACAA
AATAGAAGGAGTTGATAAAG

Downstream 100 bases:

>100_bases
ACATCCTTCGATAAACTAAAGAGACGATAGCAAGTCGGTACTGTGGAAGGAGATACATCCAGCTATCGTCTCTTTTATTT
ATGAATTTATCATTTTGACA

Product: glutamate synthase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPLNRSKKKTPMPTQKPDVRRRNFSEVALGYSEEEAVSEAQRCLQCKKPGCVEGCPVQVQIPQFIKRIAERDFEGAIKII
KETNSLPAICGRVCPQEEQCEKNCVLGKVGEPVAIGRLERFAADWERAKGIRPSVIPKKLGKKVAIIGSGPAGLTCAGDL
AKLGYDVTIFEALHKPGGVLIYGIPEFRLPKIIVEQEVEFIQQLGVEIKTNMVMGKVLTIDDLFEMGYEAVFTGTGAGLP
KFMGIPGENYLGVYSANEFLTRINLMKAYSFPNTDTPIKVGKKVAVIGGGNVAMDAARSAVRMGAEEVHIVYRRSEEEMP
ARKEEVENAKEEGIIFDLLTNPAKIIGNENGWVKGIECIRMELGEPDASGRRRPVPIMGSELVMDIDTVVIAIGTGPNPL
LTKATEGLELTKHGYIAVDEEGRTSREGVWAGGDIVTGSATVILAMGAGKKAARSIHEYLQNKKWR

Sequences:

>Translated_466_residues
MPLNRSKKKTPMPTQKPDVRRRNFSEVALGYSEEEAVSEAQRCLQCKKPGCVEGCPVQVQIPQFIKRIAERDFEGAIKII
KETNSLPAICGRVCPQEEQCEKNCVLGKVGEPVAIGRLERFAADWERAKGIRPSVIPKKLGKKVAIIGSGPAGLTCAGDL
AKLGYDVTIFEALHKPGGVLIYGIPEFRLPKIIVEQEVEFIQQLGVEIKTNMVMGKVLTIDDLFEMGYEAVFTGTGAGLP
KFMGIPGENYLGVYSANEFLTRINLMKAYSFPNTDTPIKVGKKVAVIGGGNVAMDAARSAVRMGAEEVHIVYRRSEEEMP
ARKEEVENAKEEGIIFDLLTNPAKIIGNENGWVKGIECIRMELGEPDASGRRRPVPIMGSELVMDIDTVVIAIGTGPNPL
LTKATEGLELTKHGYIAVDEEGRTSREGVWAGGDIVTGSATVILAMGAGKKAARSIHEYLQNKKWR
>Mature_465_residues
PLNRSKKKTPMPTQKPDVRRRNFSEVALGYSEEEAVSEAQRCLQCKKPGCVEGCPVQVQIPQFIKRIAERDFEGAIKIIK
ETNSLPAICGRVCPQEEQCEKNCVLGKVGEPVAIGRLERFAADWERAKGIRPSVIPKKLGKKVAIIGSGPAGLTCAGDLA
KLGYDVTIFEALHKPGGVLIYGIPEFRLPKIIVEQEVEFIQQLGVEIKTNMVMGKVLTIDDLFEMGYEAVFTGTGAGLPK
FMGIPGENYLGVYSANEFLTRINLMKAYSFPNTDTPIKVGKKVAVIGGGNVAMDAARSAVRMGAEEVHIVYRRSEEEMPA
RKEEVENAKEEGIIFDLLTNPAKIIGNENGWVKGIECIRMELGEPDASGRRRPVPIMGSELVMDIDTVVIAIGTGPNPLL
TKATEGLELTKHGYIAVDEEGRTSREGVWAGGDIVTGSATVILAMGAGKKAARSIHEYLQNKKWR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=484, Percent_Identity=33.2644628099174, Blast_Score=215, Evalue=9e-56,
Organism=Homo sapiens, GI237757300, Length=121, Percent_Identity=34.7107438016529, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788811, Length=451, Percent_Identity=39.4678492239468, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1789606, Length=459, Percent_Identity=40.958605664488, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082180, Length=476, Percent_Identity=36.344537815126, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=34.0807174887892, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789243, Length=424, Percent_Identity=33.2547169811321, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=33.7748344370861, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=27.8118609406953, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=33.125, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=475, Percent_Identity=29.2631578947368, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24640763, Length=461, Percent_Identity=34.056399132321, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI18858217, Length=461, Percent_Identity=34.056399132321, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24665547, Length=480, Percent_Identity=30.2083333333333, Blast_Score=188, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24665543, Length=480, Percent_Identity=30.2083333333333, Blast_Score=188, Evalue=7e-48,
Organism=Drosophila melanogaster, GI28574881, Length=480, Percent_Identity=30.2083333333333, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24665539, Length=480, Percent_Identity=30.2083333333333, Blast_Score=188, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50853; Mature: 50722

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNRSKKKTPMPTQKPDVRRRNFSEVALGYSEEEAVSEAQRCLQCKKPGCVEGCPVQVQ
CCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
IPQFIKRIAERDFEGAIKIIKETNSLPAICGRVCPQEEQCEKNCVLGKVGEPVAIGRLER
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHCCEECCCCCCHHHHHHHH
FAADWERAKGIRPSVIPKKLGKKVAIIGSGPAGLTCAGDLAKLGYDVTIFEALHKPGGVL
HHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEECCHHHHCCCCEEEHHHHCCCCCEE
IYGIPEFRLPKIIVEQEVEFIQQLGVEIKTNMVMGKVLTIDDLFEMGYEAVFTGTGAGLP
EECCCCCCCCHHHHHHHHHHHHHCCCEEEECEEECEEEHHHHHHHCCCCEEEECCCCCCH
KFMGIPGENYLGVYSANEFLTRINLMKAYSFPNTDTPIKVGKKVAVIGGGNVAMDAARSA
HHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
VRMGAEEVHIVYRRSEEEMPARKEEVENAKEEGIIFDLLTNPAKIIGNENGWVKGIECIR
HHCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCHHEECCCCCCCHHHHHEE
MELGEPDASGRRRPVPIMGSELVMDIDTVVIAIGTGPNPLLTKATEGLELTKHGYIAVDE
ECCCCCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHCCCCCEEECCCEEEECC
EGRTSREGVWAGGDIVTGSATVILAMGAGKKAARSIHEYLQNKKWR
CCCCCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLNRSKKKTPMPTQKPDVRRRNFSEVALGYSEEEAVSEAQRCLQCKKPGCVEGCPVQVQ
CCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
IPQFIKRIAERDFEGAIKIIKETNSLPAICGRVCPQEEQCEKNCVLGKVGEPVAIGRLER
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHCCEECCCCCCHHHHHHHH
FAADWERAKGIRPSVIPKKLGKKVAIIGSGPAGLTCAGDLAKLGYDVTIFEALHKPGGVL
HHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEECCHHHHCCCCEEEHHHHCCCCCEE
IYGIPEFRLPKIIVEQEVEFIQQLGVEIKTNMVMGKVLTIDDLFEMGYEAVFTGTGAGLP
EECCCCCCCCHHHHHHHHHHHHHCCCEEEECEEECEEEHHHHHHHCCCCEEEECCCCCCH
KFMGIPGENYLGVYSANEFLTRINLMKAYSFPNTDTPIKVGKKVAVIGGGNVAMDAARSA
HHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
VRMGAEEVHIVYRRSEEEMPARKEEVENAKEEGIIFDLLTNPAKIIGNENGWVKGIECIR
HHCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCHHEECCCCCCCHHHHHEE
MELGEPDASGRRRPVPIMGSELVMDIDTVVIAIGTGPNPLLTKATEGLELTKHGYIAVDE
ECCCCCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHCCCCCEEECCCEEEECC
EGRTSREGVWAGGDIVTGSATVILAMGAGKKAARSIHEYLQNKKWR
CCCCCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]