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The map label for this gene is amtB [C]

Identifier: 167039214

GI number: 167039214

Start: 577995

End: 579335

Strand: Direct

Name: amtB [C]

Synonym: Teth514_0555

Alternate gene names: 167039214

Gene position: 577995-579335 (Clockwise)

Preceding gene: 167039213

Following gene: 167039215

Centisome position: 23.52

GC content: 41.61

Gene sequence:

>1341_bases
ATGGATAAGGTAAAGAAAATATTTTTAAGCTTTATGATTTTAATTTTTGTAGTAACGAGTGGAATTTGCACTGCAAAAGC
AGCAGACCAAATAACACCAGAAAAAGTTGCAACAGCTATTGACAATGTTTGGGTACTTGTATCAGCTTTCTTGGTGTTCT
TCATGCAAGCAGGGTTTGCAATGGTTGAAGCGGGGTTCACAAGAGCAAAGAATGCAAGCAACATTGTTATGAAAAACCTA
ATGGACTTTGCAATTGGTTCAGTTATGTTCTGGTTGGTAGGATTTGCAATAATGTTCGGAAAAGATGCAGGTGGATTTAT
AGGAACCTCAGGGTTTTTCTTCAGTGATTCATTTGAACATTTAGAGCTTTCAATTCCTTTGATGTCCTTTTTAATTTTCC
AGACAGTATTTGCAGCAACAGCCGCAACAATTGTATCTGGTGCTATGGCTGAAAGAACAAAATTTGTTGCTTACTGCATT
TATAGTGCTGTAATTTCACTCATTATCTATCCAGTTGTAGGACACTGGGCATGGGGAGGAGGATGGTTGAGCAAATTAGG
ATTTATCGACTTTGCAGGGTCTACTGTTGTTCACTCTGTTGGCGGTTGGGCAGCGTTGATTGGTGCAAAAATGCTTGGAC
CAAGAATAGGGAAATACACAAAAGACGGAAAGGTCAATGCGATTCCAGGGCATAGCATAACATTGGCAGCGCTTGGTACC
TTCATTTTATGGTTTGGATGGTTTGGATTCAACCCAGGTTCAACACTTTCAGGTATGAGTGACAAAATTGGCGATATTGC
TGTCAATACCAATTTAGCAGCTGCAATGGGTGCAACCCTCGCGATGATTTATACATGGCTAAAGTACAAAAAACCCGATG
TTAGCATGACACTAAACGGAGCTTTGGCTGGGCTTGTTGCAATTACAGCAGGGTGTGCGTCGGTGAGCACATGGGGTGCT
GCAATAATAGGTGGGCTTGCTGGAATCTTGGTTGTTATATCTGTTGAGTTTATTGATAAAAAGCTCAAGATAGACGATCC
TGTTGGTGCAATATCGGTACATGGAGTTTGCGGTGCGTTTGGGACATTGATGGTTGGGCTTTTTGCAACAGAAGGAGGAC
TGTTCTATGGTGGTGGGATAAAGCAATTTTTAGTCCAACTTGCCGGGGTTGCTTCAACATTTGTATGGACTACTGTGACA
GCGTTTATTCTCTTTGCGATAATCAAACTCACAGTCGGTTTGCGAGTTTCCGAAGAGGAAGAGGTAGAAGGTCTGGATGT
TACAGAACATGGTGCGACAGCTTATGGTGACTTTGTAACGAAGTCTGAGGTAGTAAACTAA

Upstream 100 bases:

>100_bases
TCAAATCAATTCAAATTTGAAAAGAGGGGTGCAAGTAGGTGTACTTAATGGTTTATAAGTCTTCTTAAAACTTAATTCAA
TTATGAGGAGGTTTATAAAA

Downstream 100 bases:

>100_bases
GTTTGGTGGTTTTGTGATAAATTTTATTTGAAAAGGGTGAAGGTCAATGAAAAAGATTGAGTGTATTATCAGACCTGAAA
AGCTTGAAGAGGTAAAGGAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDKVKKIFLSFMILIFVVTSGICTAKAADQITPEKVATAIDNVWVLVSAFLVFFMQAGFAMVEAGFTRAKNASNIVMKNL
MDFAIGSVMFWLVGFAIMFGKDAGGFIGTSGFFFSDSFEHLELSIPLMSFLIFQTVFAATAATIVSGAMAERTKFVAYCI
YSAVISLIIYPVVGHWAWGGGWLSKLGFIDFAGSTVVHSVGGWAALIGAKMLGPRIGKYTKDGKVNAIPGHSITLAALGT
FILWFGWFGFNPGSTLSGMSDKIGDIAVNTNLAAAMGATLAMIYTWLKYKKPDVSMTLNGALAGLVAITAGCASVSTWGA
AIIGGLAGILVVISVEFIDKKLKIDDPVGAISVHGVCGAFGTLMVGLFATEGGLFYGGGIKQFLVQLAGVASTFVWTTVT
AFILFAIIKLTVGLRVSEEEEVEGLDVTEHGATAYGDFVTKSEVVN

Sequences:

>Translated_446_residues
MDKVKKIFLSFMILIFVVTSGICTAKAADQITPEKVATAIDNVWVLVSAFLVFFMQAGFAMVEAGFTRAKNASNIVMKNL
MDFAIGSVMFWLVGFAIMFGKDAGGFIGTSGFFFSDSFEHLELSIPLMSFLIFQTVFAATAATIVSGAMAERTKFVAYCI
YSAVISLIIYPVVGHWAWGGGWLSKLGFIDFAGSTVVHSVGGWAALIGAKMLGPRIGKYTKDGKVNAIPGHSITLAALGT
FILWFGWFGFNPGSTLSGMSDKIGDIAVNTNLAAAMGATLAMIYTWLKYKKPDVSMTLNGALAGLVAITAGCASVSTWGA
AIIGGLAGILVVISVEFIDKKLKIDDPVGAISVHGVCGAFGTLMVGLFATEGGLFYGGGIKQFLVQLAGVASTFVWTTVT
AFILFAIIKLTVGLRVSEEEEVEGLDVTEHGATAYGDFVTKSEVVN
>Mature_446_residues
MDKVKKIFLSFMILIFVVTSGICTAKAADQITPEKVATAIDNVWVLVSAFLVFFMQAGFAMVEAGFTRAKNASNIVMKNL
MDFAIGSVMFWLVGFAIMFGKDAGGFIGTSGFFFSDSFEHLELSIPLMSFLIFQTVFAATAATIVSGAMAERTKFVAYCI
YSAVISLIIYPVVGHWAWGGGWLSKLGFIDFAGSTVVHSVGGWAALIGAKMLGPRIGKYTKDGKVNAIPGHSITLAALGT
FILWFGWFGFNPGSTLSGMSDKIGDIAVNTNLAAAMGATLAMIYTWLKYKKPDVSMTLNGALAGLVAITAGCASVSTWGA
AIIGGLAGILVVISVEFIDKKLKIDDPVGAISVHGVCGAFGTLMVGLFATEGGLFYGGGIKQFLVQLAGVASTFVWTTVT
AFILFAIIKLTVGLRVSEEEEVEGLDVTEHGATAYGDFVTKSEVVN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=36.919315403423, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17550180, Length=430, Percent_Identity=37.4418604651163, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17550182, Length=430, Percent_Identity=37.2093023255814, Blast_Score=235, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=35.2668213457077, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI133931100, Length=423, Percent_Identity=34.2789598108747, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17563228, Length=341, Percent_Identity=24.9266862170088, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=416, Percent_Identity=31.0096153846154, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=30.6763285024155, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=29.7101449275362, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI161078314, Length=407, Percent_Identity=38.3292383292383, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47126; Mature: 47126

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]