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The map label for this gene is purH [H]

Identifier: 167039184

GI number: 167039184

Start: 540731

End: 542257

Strand: Direct

Name: purH [H]

Synonym: Teth514_0525

Alternate gene names: 167039184

Gene position: 540731-542257 (Clockwise)

Preceding gene: 167039183

Following gene: 167039185

Centisome position: 22.01

GC content: 35.17

Gene sequence:

>1527_bases
ATGGCCAAAAAAGCGTTAATAAGTGTTTCAAAAAAAGAAGGCATAGTTGAATTTGCCAAAAAGCTTAATGAATTAGGATA
TGAAATAATATCAACAGGTGGGACTTATAATCTTCTTAAAGAAAATAGAGTAAATGTAGTAAAGGTATCAGACATAACAG
GTTTCCCTGAAATTATGGACGGGAGAGTAAAGACGCTTCACCCTAAAATTCATGGAGGGCTTCTTGCAATAAGAGACAAT
GAAGAGCACATTAAAGCTCTTAAAGAACATGGCATTGAACCAATAGATATAGTTGTCATAAATTTATATCCTTTTAAAGA
AACAATCCTCAAAGAAAATGTGACTTTAGAAGAAGCGATAGAAAATATAGACATTGGCGGTCCTTCAATGATAAGGGCTG
CAGCTAAAAACTACAAATATGTAACTATTCTTGTAGATCCAAAGGATTATGATACAGTCATTGAAGAAATAAAACAATAT
GGCAATACAAAAGAAGAGACGAGATTTTATCTGGCAGCAAAAGCTTTTGGCCATACAGCCCTTTACGATTCTTTGATATA
CAATTATTTGATACAAAAAAATAACATAGAGTTTCCCGAAGTTATGGCCTTTGCCTATGAAAAAGCTCAAGACATGAGAT
ATGGTGAAAATCCTCATCAAAAGGCGGCTTTTTACAAAAATCCCATAAAAGCCTATGGCATTGCAGAATGTGAGCAGCTG
CACGGTAAAGAGCTCTCTTTTAACAATATAAACGATGCAAATGCCGCAATAGAACTTTTAAGAGAGTTTAAAGAGCCTGC
AGCAGTTGCCGTAAAGCACACAAATCCTTGTGGAGTAGCTATTGCAGATAATATATACAATGCTTACTTAAAAGCTTATG
AGAGTGACCCTGTTTCTATTTTTGGGGGAATTGTAGCATTAAATAGAACTGTAGATGTTAAAACAGCTGAAGAACTTATA
AAAATATTCTTAGAAATAGTAATTGCTCCAGACTTTGAAGAAGAGGCCTTTGAGATTTTAAAGAAAAAGAAAAACTTAAG
GATATTGAGGTTAAAAGAAGGATATGAAAAAGAATATGATTTAAAGAAAGTAGAAGGCGGACTTTTAGTACAAGAAAAAG
ATGAAATAGATTTAGATGAGAATAATTTGAAAGTAGTTACTAAAAAAGCACCTACGCAAAAAGAGATGGAAGATTTAAGG
TTTGCCTGGAAGGTAGTAAAGCACGTAAAATCTAATGCGATTGTTTTAGCAAAAGATGGAGCTACAGTAGGTATTGGCGT
TGGACAAGTCAACAGAATATGGCCAACAGAGCAAGCAATCAAACAAGCAGGTAGCAAAGCAAAAGGAAGTGTCCTTGCAT
CAGATGCCTTTTTCCCATTTCCAGATGTTGTGGAAGCAGCTGTAAAAGGCGGTATAACTGCCATAATCCAACCAGGCGGC
TCACAAAACGATGCTTTATCAATTGAAGCTGCAGATAAAGGCGGCGTATCAATGATATTCACAGGCATAAGGCATTTTAA
ACATTGA

Upstream 100 bases:

>100_bases
TTAAGCTTTTTACAGAGGGGAAGTTAAAAGTCGAAGGTAGAAAAGTGAAAATATTAAAGTTTTAAAAGGGATTATAAAAC
TGTAAAAAGGAGGCAATTCT

Downstream 100 bases:

>100_bases
GGAGGAATTTGTATGAAGGTTTTAGTCATAGGGGGAGGGGGAAGAGAACATGCAATTGTTCACAAACTTTCACAAAGTCC
TAAAGTGGATAAAATCTACT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAKKALISVSKKEGIVEFAKKLNELGYEIISTGGTYNLLKENRVNVVKVSDITGFPEIMDGRVKTLHPKIHGGLLAIRDN
EEHIKALKEHGIEPIDIVVINLYPFKETILKENVTLEEAIENIDIGGPSMIRAAAKNYKYVTILVDPKDYDTVIEEIKQY
GNTKEETRFYLAAKAFGHTALYDSLIYNYLIQKNNIEFPEVMAFAYEKAQDMRYGENPHQKAAFYKNPIKAYGIAECEQL
HGKELSFNNINDANAAIELLREFKEPAAVAVKHTNPCGVAIADNIYNAYLKAYESDPVSIFGGIVALNRTVDVKTAEELI
KIFLEIVIAPDFEEEAFEILKKKKNLRILRLKEGYEKEYDLKKVEGGLLVQEKDEIDLDENNLKVVTKKAPTQKEMEDLR
FAWKVVKHVKSNAIVLAKDGATVGIGVGQVNRIWPTEQAIKQAGSKAKGSVLASDAFFPFPDVVEAAVKGGITAIIQPGG
SQNDALSIEAADKGGVSMIFTGIRHFKH

Sequences:

>Translated_508_residues
MAKKALISVSKKEGIVEFAKKLNELGYEIISTGGTYNLLKENRVNVVKVSDITGFPEIMDGRVKTLHPKIHGGLLAIRDN
EEHIKALKEHGIEPIDIVVINLYPFKETILKENVTLEEAIENIDIGGPSMIRAAAKNYKYVTILVDPKDYDTVIEEIKQY
GNTKEETRFYLAAKAFGHTALYDSLIYNYLIQKNNIEFPEVMAFAYEKAQDMRYGENPHQKAAFYKNPIKAYGIAECEQL
HGKELSFNNINDANAAIELLREFKEPAAVAVKHTNPCGVAIADNIYNAYLKAYESDPVSIFGGIVALNRTVDVKTAEELI
KIFLEIVIAPDFEEEAFEILKKKKNLRILRLKEGYEKEYDLKKVEGGLLVQEKDEIDLDENNLKVVTKKAPTQKEMEDLR
FAWKVVKHVKSNAIVLAKDGATVGIGVGQVNRIWPTEQAIKQAGSKAKGSVLASDAFFPFPDVVEAAVKGGITAIIQPGG
SQNDALSIEAADKGGVSMIFTGIRHFKH
>Mature_507_residues
AKKALISVSKKEGIVEFAKKLNELGYEIISTGGTYNLLKENRVNVVKVSDITGFPEIMDGRVKTLHPKIHGGLLAIRDNE
EHIKALKEHGIEPIDIVVINLYPFKETILKENVTLEEAIENIDIGGPSMIRAAAKNYKYVTILVDPKDYDTVIEEIKQYG
NTKEETRFYLAAKAFGHTALYDSLIYNYLIQKNNIEFPEVMAFAYEKAQDMRYGENPHQKAAFYKNPIKAYGIAECEQLH
GKELSFNNINDANAAIELLREFKEPAAVAVKHTNPCGVAIADNIYNAYLKAYESDPVSIFGGIVALNRTVDVKTAEELIK
IFLEIVIAPDFEEEAFEILKKKKNLRILRLKEGYEKEYDLKKVEGGLLVQEKDEIDLDENNLKVVTKKAPTQKEMEDLRF
AWKVVKHVKSNAIVLAKDGATVGIGVGQVNRIWPTEQAIKQAGSKAKGSVLASDAFFPFPDVVEAAVKGGITAIIQPGGS
QNDALSIEAADKGGVSMIFTGIRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=482, Percent_Identity=37.7593360995851, Blast_Score=261, Evalue=8e-70,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=50.763358778626, Blast_Score=521, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=32.6732673267327, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71985574, Length=311, Percent_Identity=29.2604501607717, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=38.5106382978723, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6323768, Length=478, Percent_Identity=37.0292887029289, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24649832, Length=471, Percent_Identity=37.791932059448, Blast_Score=288, Evalue=9e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56560; Mature: 56428

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKALISVSKKEGIVEFAKKLNELGYEIISTGGTYNLLKENRVNVVKVSDITGFPEIMD
CCCHHHEEEHHHCCHHHHHHHHHHCCHHEEECCCCEEEHHCCCCCEEEEECCCCCHHHHC
GRVKTLHPKIHGGLLAIRDNEEHIKALKEHGIEPIDIVVINLYPFKETILKENVTLEEAI
CCCEEECHHHCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCCHHHHH
ENIDIGGPSMIRAAAKNYKYVTILVDPKDYDTVIEEIKQYGNTKEETRFYLAAKAFGHTA
HHCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHEEEEEHHHHCHHH
LYDSLIYNYLIQKNNIEFPEVMAFAYEKAQDMRYGENPHQKAAFYKNPIKAYGIAECEQL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHCCCHHHHHH
HGKELSFNNINDANAAIELLREFKEPAAVAVKHTNPCGVAIADNIYNAYLKAYESDPVSI
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCCCHHH
FGGIVALNRTVDVKTAEELIKIFLEIVIAPDFEEEAFEILKKKKNLRILRLKEGYEKEYD
HHHEEEECCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
LKKVEGGLLVQEKDEIDLDENNLKVVTKKAPTQKEMEDLRFAWKVVKHVKSNAIVLAKDG
HHHCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
ATVGIGVGQVNRIWPTEQAIKQAGSKAKGSVLASDAFFPFPDVVEAAVKGGITAIIQPGG
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEEECCCC
SQNDALSIEAADKGGVSMIFTGIRHFKH
CCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKALISVSKKEGIVEFAKKLNELGYEIISTGGTYNLLKENRVNVVKVSDITGFPEIMD
CCHHHEEEHHHCCHHHHHHHHHHCCHHEEECCCCEEEHHCCCCCEEEEECCCCCHHHHC
GRVKTLHPKIHGGLLAIRDNEEHIKALKEHGIEPIDIVVINLYPFKETILKENVTLEEAI
CCCEEECHHHCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCCHHHHH
ENIDIGGPSMIRAAAKNYKYVTILVDPKDYDTVIEEIKQYGNTKEETRFYLAAKAFGHTA
HHCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHEEEEEHHHHCHHH
LYDSLIYNYLIQKNNIEFPEVMAFAYEKAQDMRYGENPHQKAAFYKNPIKAYGIAECEQL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHCCCHHHHHH
HGKELSFNNINDANAAIELLREFKEPAAVAVKHTNPCGVAIADNIYNAYLKAYESDPVSI
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCCCHHH
FGGIVALNRTVDVKTAEELIKIFLEIVIAPDFEEEAFEILKKKKNLRILRLKEGYEKEYD
HHHEEEECCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
LKKVEGGLLVQEKDEIDLDENNLKVVTKKAPTQKEMEDLRFAWKVVKHVKSNAIVLAKDG
HHHCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
ATVGIGVGQVNRIWPTEQAIKQAGSKAKGSVLASDAFFPFPDVVEAAVKGGITAIIQPGG
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEEECCCC
SQNDALSIEAADKGGVSMIFTGIRHFKH
CCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA