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The map label for this gene is mjaIIIR [H]

Identifier: 167039120

GI number: 167039120

Start: 472676

End: 473599

Strand: Direct

Name: mjaIIIR [H]

Synonym: Teth514_0459

Alternate gene names: 167039120

Gene position: 472676-473599 (Clockwise)

Preceding gene: 167039119

Following gene: 167039121

Centisome position: 19.24

GC content: 28.68

Gene sequence:

>924_bases
ATGAAGGAATTATATATTGAATTCTTAAATGTAGAAAATGAGGAAAGTGTAATAGAAAAATTTCATGATACACTTGTGGA
CACAAATAGGAGTTATGACTTTTTTGTTGATTGGGACAAAGTAAGAAAACACGTTGAAGAACATAAAATAGAATTTAATA
TATTAAATAGTTTAATAGGAAGTAATCGGTTTGATAATGACTTAAGAAAAATTTTATTAAATTATCCTCGAGTGCTTCCT
GTTATACCGATTTTATTAGCAATACGAGACCTTAATTTTAAAGTAATTCGCGATTTTACAGATAAAAACTTGGATATTGT
GAATTATGACTTTAACACTAGAACTCTTACATCAGAAGATATTGATAATATTATAGAATTTTTTGATAAGACAGGCTTAA
AGAGATTTTTTCTGGAAATGGGTACGAAGAGCGTACAAGATTATGTAATAGGTGTGGAAGTAGGCATGGATACCCATGCG
AGGAAAAATAGAAGTGGAGATGCGATGGAATTATTATTAAAGCCAATTATTGAAGATATAGCAACAAAGGAAAAAGGATT
TGAAAGAGTTTTATTTCAGAAAAATTTCGGATATTTGGAAACTAATTATGGTATAGAGGTTAACTCTTCAATTAAAAACA
GAAAAGCTGATTTTATTTTAATCAAAGACAACAAAAAAGTTATCAATATCGAAGTGAATTTTTATTCAGGAACTGGTTCA
AAACCTCAAGAAATAGTGGATTCCTACATAGAAAGACAGAATGAACTTAAAAATAATGGATTTGAATTTATTTGGATTAC
AGACGGAGAGGGATGGAGAGGACAGAAAAATCAAATTCATAAAGGATTTGAGAAAATAGATTATTTGTTAAATCTTCATT
TTGTGCGTATTGGTTTATTGGAGGAAATATTATGCAGGATATAG

Upstream 100 bases:

>100_bases
ATTGAAAGGGTAAAAGCAAAAAGATATATTAACAGTAATGGGGATAAACGAGGAGATATAAGTGAATTGATAATAAGGAA
TTATGAATAGGGGGCTTAAA

Downstream 100 bases:

>100_bases
ATTTTAAAAAAGAAATAACTACTGTATGGTCGTTTCCTGAAAGGGGAAAATGGAAAACGCATAAGGGAAATTACAGAGGC
AATTTTGCACCTCAAATTCC

Product: Type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.MjaIII; Endonuclease MjaIII; Type II restriction enzyme MjaIII [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKELYIEFLNVENEESVIEKFHDTLVDTNRSYDFFVDWDKVRKHVEEHKIEFNILNSLIGSNRFDNDLRKILLNYPRVLP
VIPILLAIRDLNFKVIRDFTDKNLDIVNYDFNTRTLTSEDIDNIIEFFDKTGLKRFFLEMGTKSVQDYVIGVEVGMDTHA
RKNRSGDAMELLLKPIIEDIATKEKGFERVLFQKNFGYLETNYGIEVNSSIKNRKADFILIKDNKKVINIEVNFYSGTGS
KPQEIVDSYIERQNELKNNGFEFIWITDGEGWRGQKNQIHKGFEKIDYLLNLHFVRIGLLEEILCRI

Sequences:

>Translated_307_residues
MKELYIEFLNVENEESVIEKFHDTLVDTNRSYDFFVDWDKVRKHVEEHKIEFNILNSLIGSNRFDNDLRKILLNYPRVLP
VIPILLAIRDLNFKVIRDFTDKNLDIVNYDFNTRTLTSEDIDNIIEFFDKTGLKRFFLEMGTKSVQDYVIGVEVGMDTHA
RKNRSGDAMELLLKPIIEDIATKEKGFERVLFQKNFGYLETNYGIEVNSSIKNRKADFILIKDNKKVINIEVNFYSGTGS
KPQEIVDSYIERQNELKNNGFEFIWITDGEGWRGQKNQIHKGFEKIDYLLNLHFVRIGLLEEILCRI
>Mature_307_residues
MKELYIEFLNVENEESVIEKFHDTLVDTNRSYDFFVDWDKVRKHVEEHKIEFNILNSLIGSNRFDNDLRKILLNYPRVLP
VIPILLAIRDLNFKVIRDFTDKNLDIVNYDFNTRTLTSEDIDNIIEFFDKTGLKRFFLEMGTKSVQDYVIGVEVGMDTHA
RKNRSGDAMELLLKPIIEDIATKEKGFERVLFQKNFGYLETNYGIEVNSSIKNRKADFILIKDNKKVINIEVNFYSGTGS
KPQEIVDSYIERQNELKNNGFEFIWITDGEGWRGQKNQIHKGFEKIDYLLNLHFVRIGLLEEILCRI

Specific function: Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme DpnII family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021191
- InterPro:   IPR007637 [H]

Pfam domain/function: PF04556 DpmII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 36167; Mature: 36167

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELYIEFLNVENEESVIEKFHDTLVDTNRSYDFFVDWDKVRKHVEEHKIEFNILNSLIG
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
SNRFDNDLRKILLNYPRVLPVIPILLAIRDLNFKVIRDFTDKNLDIVNYDFNTRTLTSED
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEEECCCCCEECHHH
IDNIIEFFDKTGLKRFFLEMGTKSVQDYVIGVEVGMDTHARKNRSGDAMELLLKPIIEDI
HHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ATKEKGFERVLFQKNFGYLETNYGIEVNSSIKNRKADFILIKDNKKVINIEVNFYSGTGS
HHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCEEEEECCCEEEEEEEEEECCCCC
KPQEIVDSYIERQNELKNNGFEFIWITDGEGWRGQKNQIHKGFEKIDYLLNLHFVRIGLL
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEILCRI
HHHHHCC
>Mature Secondary Structure
MKELYIEFLNVENEESVIEKFHDTLVDTNRSYDFFVDWDKVRKHVEEHKIEFNILNSLIG
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
SNRFDNDLRKILLNYPRVLPVIPILLAIRDLNFKVIRDFTDKNLDIVNYDFNTRTLTSED
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEEECCCCCEECHHH
IDNIIEFFDKTGLKRFFLEMGTKSVQDYVIGVEVGMDTHARKNRSGDAMELLLKPIIEDI
HHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ATKEKGFERVLFQKNFGYLETNYGIEVNSSIKNRKADFILIKDNKKVINIEVNFYSGTGS
HHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCEEEEECCCEEEEEEEEEECCCCC
KPQEIVDSYIERQNELKNNGFEFIWITDGEGWRGQKNQIHKGFEKIDYLLNLHFVRIGLL
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEILCRI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]