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The map label for this gene is mjaIIIM [H]

Identifier: 167039119

GI number: 167039119

Start: 471712

End: 472665

Strand: Direct

Name: mjaIIIM [H]

Synonym: Teth514_0458

Alternate gene names: 167039119

Gene position: 471712-472665 (Clockwise)

Preceding gene: 167039117

Following gene: 167039120

Centisome position: 19.2

GC content: 29.56

Gene sequence:

>954_bases
TTGAATTTGATAGAGATTTCAGACAAATCATCAAGCATTTTAAATAATGCTAAGCCTTTTTTAAAATGGGCAGGCGGAAA
AACACAGTTATTAGATGAGTTATATAAGAGGTTGCCATCTTCTTTAATTCAAAGTGGTGAAATAGAGAGATATGTTGAGC
CTTTTGTTGGTGGTGGTGCATTTTTCTTCTTTTTAAAAAGAAATTTCAAATTTAAGGAATCGTTTTTAATCGATATAAAT
AAAGAACTAATAATAGGCTATAAAGTAATTCAAAATAATGTGAATGAATTAATTGATGAGTTAAGTAGCATGGAAGAGAA
ATATCTAAAATTATCAGAGGAACAAAGGAAAGATTTTTACTATAACATTAGAGATGAGTATAATAGACAAAAGGATAATT
TTGATTATGTAAATTACAATTTGGACTGGGTAAAAAGAGCAGCGGACTTAATTTTTTTAAACAAGACATGTTTTAATGGA
TTATATCGTTTAAATAAAAAAGGTGAATTCAATGTACCATTTGGGAAATATAAAAACCCTACTATTTGTGATGCTGACAA
TTTAATCGAAGTAAGTAAAGCGTTAGAGAATACAGAGGTCATTTGTGCAGATTTTGAAGAAAGCAAAAAGTATATACATA
AAAACACTTTGGTTTATCTCGATCCTCCTTATCGCCCTTTAAACAATACTTCGAATTTTACAAGTTATAATGAGAACGGG
TTTGATGATGATGACCAGAGGCGGTTAGCTCAGTTTTTTAAAGAAATGGATAAAAAAGGCGCATATCTTATACTTAGCAA
TTCGGACCCCAAGAATGAAGATGTAGATGACAATTTTTTTGATGAATTGTATGCAGGGTTTATTATTGAAAGGGTAAAAG
CAAAAAGATATATTAACAGTAATGGGGATAAACGAGGAGATATAAGTGAATTGATAATAAGGAATTATGAATAG

Upstream 100 bases:

>100_bases
ATTAGGAGAAATTGCGAACAAAACTTTTTCACTTTAAAAATTGATGATATAATTATTTTAAGAGACAAGCTTAAAGGGAA
GTGATGGTGAAGTGCAAACA

Downstream 100 bases:

>100_bases
GGGGCTTAAAATGAAGGAATTATATATTGAATTCTTAAATGTAGAAAATGAGGAAAGTGTAATAGAAAAATTTCATGATA
CACTTGTGGACACAAATAGG

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNLIEISDKSSSILNNAKPFLKWAGGKTQLLDELYKRLPSSLIQSGEIERYVEPFVGGGAFFFFLKRNFKFKESFLIDIN
KELIIGYKVIQNNVNELIDELSSMEEKYLKLSEEQRKDFYYNIRDEYNRQKDNFDYVNYNLDWVKRAADLIFLNKTCFNG
LYRLNKKGEFNVPFGKYKNPTICDADNLIEVSKALENTEVICADFEESKKYIHKNTLVYLDPPYRPLNNTSNFTSYNENG
FDDDDQRRLAQFFKEMDKKGAYLILSNSDPKNEDVDDNFFDELYAGFIIERVKAKRYINSNGDKRGDISELIIRNYE

Sequences:

>Translated_317_residues
MNLIEISDKSSSILNNAKPFLKWAGGKTQLLDELYKRLPSSLIQSGEIERYVEPFVGGGAFFFFLKRNFKFKESFLIDIN
KELIIGYKVIQNNVNELIDELSSMEEKYLKLSEEQRKDFYYNIRDEYNRQKDNFDYVNYNLDWVKRAADLIFLNKTCFNG
LYRLNKKGEFNVPFGKYKNPTICDADNLIEVSKALENTEVICADFEESKKYIHKNTLVYLDPPYRPLNNTSNFTSYNENG
FDDDDQRRLAQFFKEMDKKGAYLILSNSDPKNEDVDDNFFDELYAGFIIERVKAKRYINSNGDKRGDISELIIRNYE
>Mature_317_residues
MNLIEISDKSSSILNNAKPFLKWAGGKTQLLDELYKRLPSSLIQSGEIERYVEPFVGGGAFFFFLKRNFKFKESFLIDIN
KELIIGYKVIQNNVNELIDELSSMEEKYLKLSEEQRKDFYYNIRDEYNRQKDNFDYVNYNLDWVKRAADLIFLNKTCFNG
LYRLNKKGEFNVPFGKYKNPTICDADNLIEVSKALENTEVICADFEESKKYIHKNTLVYLDPPYRPLNNTSNFTSYNENG
FDDDDQRRLAQFFKEMDKKGAYLILSNSDPKNEDVDDNFFDELYAGFIIERVKAKRYINSNGDKRGDISELIIRNYE

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=295, Percent_Identity=33.5593220338983, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 37304; Mature: 37304

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIEISDKSSSILNNAKPFLKWAGGKTQLLDELYKRLPSSLIQSGEIERYVEPFVGGGA
CCEEEECCCHHHHHHCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
FFFFLKRNFKFKESFLIDINKELIIGYKVIQNNVNELIDELSSMEEKYLKLSEEQRKDFY
EEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YNIRDEYNRQKDNFDYVNYNLDWVKRAADLIFLNKTCFNGLYRLNKKGEFNVPFGKYKNP
EECHHHHCCCCCCCCEEECCHHHHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
TICDADNLIEVSKALENTEVICADFEESKKYIHKNTLVYLDPPYRPLNNTSNFTSYNENG
CEECCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCC
FDDDDQRRLAQFFKEMDKKGAYLILSNSDPKNEDVDDNFFDELYAGFIIERVKAKRYINS
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NGDKRGDISELIIRNYE
CCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MNLIEISDKSSSILNNAKPFLKWAGGKTQLLDELYKRLPSSLIQSGEIERYVEPFVGGGA
CCEEEECCCHHHHHHCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
FFFFLKRNFKFKESFLIDINKELIIGYKVIQNNVNELIDELSSMEEKYLKLSEEQRKDFY
EEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YNIRDEYNRQKDNFDYVNYNLDWVKRAADLIFLNKTCFNGLYRLNKKGEFNVPFGKYKNP
EECHHHHCCCCCCCCEEECCHHHHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
TICDADNLIEVSKALENTEVICADFEESKKYIHKNTLVYLDPPYRPLNNTSNFTSYNENG
CEECCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCC
FDDDDQRRLAQFFKEMDKKGAYLILSNSDPKNEDVDDNFFDELYAGFIIERVKAKRYINS
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NGDKRGDISELIIRNYE
CCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]