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The map label for this gene is dnaN [H]

Identifier: 167038677

GI number: 167038677

Start: 1762

End: 2874

Strand: Direct

Name: dnaN [H]

Synonym: Teth514_0002

Alternate gene names: 167038677

Gene position: 1762-2874 (Clockwise)

Preceding gene: 167038676

Following gene: 167038678

Centisome position: 0.07

GC content: 31.18

Gene sequence:

>1113_bases
ATGAAATTTGTGTGTGATAAAAATTCATTATTTGAAGGAGTAAATATTGCAATAAGGGGGGTATCTTCCCGCACTACTCT
TCCTATTCTTCAAGGTATAAAAATTTCGGCTTTGAAAGGTCAAGTCAAATTTTCAGGAACTGATCTGGACATAGGAATAG
AGTGTCAAATTCCTGCAGTGATAGAAGAAGAGGGAGAAATAGTAGTACCTGCAAAAATTTTCTCAGAATTAGTTCGTAAA
TTGCCTGAGGGAGATGTAAAAATAGAAAGTGATTCTCAAAACACGGTAAATGTAGTTTGCGATAGTATAAATTTCACTAT
TGCAGGAAGTGACGCTGTAGAGTTTCCTGAAATTCCTCTAGTATCTAAAGAAAATTCTTTTAAGTTGACGCAAAATATTC
TAAAAGACTTAATAAGAAAAACAGTCTTTTGTATTGCCCAGGAGCAAACAAGACCTATTTTGACAGGTGTTCTATTTGAA
GTGTTTCAAACTGAAGTTAAAGCAGTAGCTTTAGATGGATTTAGAATGGCTATATATTCTTATCAGAGTGAGGAAAAATT
CTTTGACGAGGATATAGAAAAATATTCTATAGTAATTCCAGGTAAAACTTTAGAAGAGATATACAGAATCTTAAAAGATG
AGGAAACAGAGATAGAAATATACCACACTGCTAATCAAGTGTTATTCCAAATTGAAAATACAAAGGTAATATCGAGGCTT
TTAGAAGGAAGCTTTATAAACTACAATGCTGTTTTACCAAAGGATTATAAGACTGAAGTGGTAGTCAAAAGAGAAGTTTT
AACTGAAAGTATTGAAAGAGCTTCACTAATTGCAGAAAGCAAAAATAATTTGATTAAGTTTGAGATTGGTGATAAATTTA
TTACTGTCTCTTCTAATTCTGAAAAGGGCAAGATGATAGAAAAAATAGAAGTTGATGTAAAAGGAATGTTTCTTGAGATC
GCTTTTAACTCAAGATATTTATTAGATTCTTTAAGAGCCATCGATGAAGAAGAAATAACGCTTTATTTCATAAATAGTAT
AAATCCGTTAATAATAAAACCTATAGGAAGTAAAGATTATCTTTATATGATACTTCCTGTAAAGCTCAATTGA

Upstream 100 bases:

>100_bases
AACAAACTAACATCTTCTATTACTATGACTACTAAACGCTTATCTTATCTATTATATGATTTGACTCAAAAAAATATTCT
CAAAAACAGGAGTGAAAAAA

Downstream 100 bases:

>100_bases
AAATAAGAGGTGTTTTTTAAATGATAGAAGTGCCTATAGAGACGGAATATATTACCTTAGGGCAATTTTTAAAGTATATG
AAAATATGTCAAACTGGAGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKFVCDKNSLFEGVNIAIRGVSSRTTLPILQGIKISALKGQVKFSGTDLDIGIECQIPAVIEEEGEIVVPAKIFSELVRK
LPEGDVKIESDSQNTVNVVCDSINFTIAGSDAVEFPEIPLVSKENSFKLTQNILKDLIRKTVFCIAQEQTRPILTGVLFE
VFQTEVKAVALDGFRMAIYSYQSEEKFFDEDIEKYSIVIPGKTLEEIYRILKDEETEIEIYHTANQVLFQIENTKVISRL
LEGSFINYNAVLPKDYKTEVVVKREVLTESIERASLIAESKNNLIKFEIGDKFITVSSNSEKGKMIEKIEVDVKGMFLEI
AFNSRYLLDSLRAIDEEEITLYFINSINPLIIKPIGSKDYLYMILPVKLN

Sequences:

>Translated_370_residues
MKFVCDKNSLFEGVNIAIRGVSSRTTLPILQGIKISALKGQVKFSGTDLDIGIECQIPAVIEEEGEIVVPAKIFSELVRK
LPEGDVKIESDSQNTVNVVCDSINFTIAGSDAVEFPEIPLVSKENSFKLTQNILKDLIRKTVFCIAQEQTRPILTGVLFE
VFQTEVKAVALDGFRMAIYSYQSEEKFFDEDIEKYSIVIPGKTLEEIYRILKDEETEIEIYHTANQVLFQIENTKVISRL
LEGSFINYNAVLPKDYKTEVVVKREVLTESIERASLIAESKNNLIKFEIGDKFITVSSNSEKGKMIEKIEVDVKGMFLEI
AFNSRYLLDSLRAIDEEEITLYFINSINPLIIKPIGSKDYLYMILPVKLN
>Mature_370_residues
MKFVCDKNSLFEGVNIAIRGVSSRTTLPILQGIKISALKGQVKFSGTDLDIGIECQIPAVIEEEGEIVVPAKIFSELVRK
LPEGDVKIESDSQNTVNVVCDSINFTIAGSDAVEFPEIPLVSKENSFKLTQNILKDLIRKTVFCIAQEQTRPILTGVLFE
VFQTEVKAVALDGFRMAIYSYQSEEKFFDEDIEKYSIVIPGKTLEEIYRILKDEETEIEIYHTANQVLFQIENTKVISRL
LEGSFINYNAVLPKDYKTEVVVKREVLTESIERASLIAESKNNLIKFEIGDKFITVSSNSEKGKMIEKIEVDVKGMFLEI
AFNSRYLLDSLRAIDEEEITLYFINSINPLIIKPIGSKDYLYMILPVKLN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=372, Percent_Identity=26.0752688172043, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41853; Mature: 41853

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVCDKNSLFEGVNIAIRGVSSRTTLPILQGIKISALKGQVKFSGTDLDIGIECQIPAV
CCEEECCCCCCCCCEEEEEECCCCCCCHHHCCEEEEEEECEEEEECCCEEEEEEEECCCE
IEEEGEIVVPAKIFSELVRKLPEGDVKIESDSQNTVNVVCDSINFTIAGSDAVEFPEIPL
ECCCCCEEECHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECEEEEEECCCCCCCCCCCE
VSKENSFKLTQNILKDLIRKTVFCIAQEQTRPILTGVLFEVFQTEVKAVALDGFRMAIYS
ECCCCCHHHHHHHHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEE
YQSEEKFFDEDIEKYSIVIPGKTLEEIYRILKDEETEIEIYHTANQVLFQIENTKVISRL
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEEECCHHHHHHH
LEGSFINYNAVLPKDYKTEVVVKREVLTESIERASLIAESKNNLIKFEIGDKFITVSSNS
HCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCC
EKGKMIEKIEVDVKGMFLEIAFNSRYLLDSLRAIDEEEITLYFINSINPLIIKPIGSKDY
CCCCEEEEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCE
LYMILPVKLN
EEEEEEEEEC
>Mature Secondary Structure
MKFVCDKNSLFEGVNIAIRGVSSRTTLPILQGIKISALKGQVKFSGTDLDIGIECQIPAV
CCEEECCCCCCCCCEEEEEECCCCCCCHHHCCEEEEEEECEEEEECCCEEEEEEEECCCE
IEEEGEIVVPAKIFSELVRKLPEGDVKIESDSQNTVNVVCDSINFTIAGSDAVEFPEIPL
ECCCCCEEECHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECEEEEEECCCCCCCCCCCE
VSKENSFKLTQNILKDLIRKTVFCIAQEQTRPILTGVLFEVFQTEVKAVALDGFRMAIYS
ECCCCCHHHHHHHHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEE
YQSEEKFFDEDIEKYSIVIPGKTLEEIYRILKDEETEIEIYHTANQVLFQIENTKVISRL
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEEECCHHHHHHH
LEGSFINYNAVLPKDYKTEVVVKREVLTESIERASLIAESKNNLIKFEIGDKFITVSSNS
HCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCC
EKGKMIEKIEVDVKGMFLEIAFNSRYLLDSLRAIDEEEITLYFINSINPLIIKPIGSKDY
CCCCEEEEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCE
LYMILPVKLN
EEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]