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The map label for this gene is proB [H]

Identifier: 163845421

GI number: 163845421

Start: 1283271

End: 1284407

Strand: Reverse

Name: proB [H]

Synonym: BSUIS_B1322

Alternate gene names: 163845421

Gene position: 1284407-1283271 (Counterclockwise)

Preceding gene: 163845422

Following gene: 163845420

Centisome position: 91.69

GC content: 60.25

Gene sequence:

>1137_bases
ATGCTGAAGAAACTAAAAGACTATCGCCGCATCGTTGTTAAAATAGGCTCTGCCCTTCTGGTGGACCGGGCCACAGGCCT
GAAGCGCGAATGGCTGGAAAGCCTGGGGCAGGATATTGCCGCCTTGCAGCATGCGGGCGTTGAGGTGCTGGTGGTTTCGT
CGGGTGCAATTGCACTGGGGCGCACGGTTCTGGGCCTGCCGAAAAAGGCGCTGAAGCTTGAGGAAAGCCAGGCGGCGGCA
GCAGCCGGGCAGATTGCGCTGGCGAAGGCTTATGCGGATGTGCTGGGCGGACATGGCATCAAGTCGGGACAGATTCTGGT
CACGCTTTCCGATACGGAAGAGCGCCGCCGCTATCTCAATGCGCGCGCAACCATCGAGACCCTGCTGAAGCTCAAGGCCG
TGCCGATCATCAATGAAAACGATACGGTGGCAACCACCGAAATCCGTTATGGCGACAATGACAGGTTGGCGGCGCGCGTC
GCGACCATGATGGGCGCGGACCTTCTGATCCTGCTTTCCGACATTGACGGGCTTTATACCGCGCCGCCGCACAAGAATCC
GGATGCGCAATTTCTGCCCTTTGTCGAAACCATCACGCCGCAGATCGAAGCCATGGCGGGGGCGGCAGCATCCGAGCTTT
CGCGGGGTGGCATGAAGACCAAGCTCGATGCGGGCAAGATTGCAAACGCTGCCGGGACCGCGATGATTATCACTTCGGGC
ACGCGTTTCGGCCCGCTTTCGGCAATTGACCGAGGGGAGCGTGCGACATTGTTCGAGGCTGCCCATGCGCCGGTCAATGC
GTGGAAGACGTGGATTTCCGGCAATCTGGAACCGGCTGGCCGGTTGACTGTGGATGCGGGTGCTGTCAAGGCGCTCAAAT
CCGGCAAATCGCTTTTGCCTGCGGGCGTGAAGGAAGTTGACGGGGATTTCGAGCGTGGCGACACGGTTGCCGTCATGAAC
GAGGATGGGCGGGAGATTGCGCGCGGTCTGATCGCCTATGATGCTGCTGACGCCCGCAAGGTTGCCGGGCATAAAAGCGA
TGAGATCAGTGCCATTCTTGGCTATGATGCCCGCGCGGCGATGATCCATCGCAACGATCTGGTGGTGCGGGCCGCCAGCG
ATGCGAAAGCCGCATAG

Upstream 100 bases:

>100_bases
GCTGCGGCAACTTGCGCGTATCATTGATCTGAGCCGCGCCGAAGAGGCTGGAACGGCGCAAGCCGAAGAGTAAGGCTTCT
GCGGCGAGAGGAATGAAACC

Downstream 100 bases:

>100_bases
GGGGATAGGATGTTGGTCAAGGCGGATATGACGAAGGATATTGCGCAAGTGATGGCGGAGGTTGGCCGCAAGGCAAAAGC
CGCTGCGGCTCCTCTCTCCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLKKLKDYRRIVVKIGSALLVDRATGLKREWLESLGQDIAALQHAGVEVLVVSSGAIALGRTVLGLPKKALKLEESQAAA
AAGQIALAKAYADVLGGHGIKSGQILVTLSDTEERRRYLNARATIETLLKLKAVPIINENDTVATTEIRYGDNDRLAARV
ATMMGADLLILLSDIDGLYTAPPHKNPDAQFLPFVETITPQIEAMAGAAASELSRGGMKTKLDAGKIANAAGTAMIITSG
TRFGPLSAIDRGERATLFEAAHAPVNAWKTWISGNLEPAGRLTVDAGAVKALKSGKSLLPAGVKEVDGDFERGDTVAVMN
EDGREIARGLIAYDAADARKVAGHKSDEISAILGYDARAAMIHRNDLVVRAASDAKAA

Sequences:

>Translated_378_residues
MLKKLKDYRRIVVKIGSALLVDRATGLKREWLESLGQDIAALQHAGVEVLVVSSGAIALGRTVLGLPKKALKLEESQAAA
AAGQIALAKAYADVLGGHGIKSGQILVTLSDTEERRRYLNARATIETLLKLKAVPIINENDTVATTEIRYGDNDRLAARV
ATMMGADLLILLSDIDGLYTAPPHKNPDAQFLPFVETITPQIEAMAGAAASELSRGGMKTKLDAGKIANAAGTAMIITSG
TRFGPLSAIDRGERATLFEAAHAPVNAWKTWISGNLEPAGRLTVDAGAVKALKSGKSLLPAGVKEVDGDFERGDTVAVMN
EDGREIARGLIAYDAADARKVAGHKSDEISAILGYDARAAMIHRNDLVVRAASDAKAA
>Mature_378_residues
MLKKLKDYRRIVVKIGSALLVDRATGLKREWLESLGQDIAALQHAGVEVLVVSSGAIALGRTVLGLPKKALKLEESQAAA
AAGQIALAKAYADVLGGHGIKSGQILVTLSDTEERRRYLNARATIETLLKLKAVPIINENDTVATTEIRYGDNDRLAARV
ATMMGADLLILLSDIDGLYTAPPHKNPDAQFLPFVETITPQIEAMAGAAASELSRGGMKTKLDAGKIANAAGTAMIITSG
TRFGPLSAIDRGERATLFEAAHAPVNAWKTWISGNLEPAGRLTVDAGAVKALKSGKSLLPAGVKEVDGDFERGDTVAVMN
EDGREIARGLIAYDAADARKVAGHKSDEISAILGYDARAAMIHRNDLVVRAASDAKAA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=270, Percent_Identity=34.0740740740741, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI21361368, Length=272, Percent_Identity=33.8235294117647, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=42.9347826086957, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17569967, Length=256, Percent_Identity=34.765625, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=256, Percent_Identity=34.765625, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=30.4347826086957, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=31.2348668280872, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=31.986531986532, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39874; Mature: 39874

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLKDYRRIVVKIGSALLVDRATGLKREWLESLGQDIAALQHAGVEVLVVSSGAIALG
CCCHHHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
RTVLGLPKKALKLEESQAAAAAGQIALAKAYADVLGGHGIKSGQILVTLSDTEERRRYLN
HHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
ARATIETLLKLKAVPIINENDTVATTEIRYGDNDRLAARVATMMGADLLILLSDIDGLYT
HHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
APPHKNPDAQFLPFVETITPQIEAMAGAAASELSRGGMKTKLDAGKIANAAGTAMIITSG
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECC
TRFGPLSAIDRGERATLFEAAHAPVNAWKTWISGNLEPAGRLTVDAGAVKALKSGKSLLP
CCCCCHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCC
AGVKEVDGDFERGDTVAVMNEDGREIARGLIAYDAADARKVAGHKSDEISAILGYDARAA
CCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCCEEE
MIHRNDLVVRAASDAKAA
EEECCCEEEEECCCCCCC
>Mature Secondary Structure
MLKKLKDYRRIVVKIGSALLVDRATGLKREWLESLGQDIAALQHAGVEVLVVSSGAIALG
CCCHHHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
RTVLGLPKKALKLEESQAAAAAGQIALAKAYADVLGGHGIKSGQILVTLSDTEERRRYLN
HHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
ARATIETLLKLKAVPIINENDTVATTEIRYGDNDRLAARVATMMGADLLILLSDIDGLYT
HHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
APPHKNPDAQFLPFVETITPQIEAMAGAAASELSRGGMKTKLDAGKIANAAGTAMIITSG
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECC
TRFGPLSAIDRGERATLFEAAHAPVNAWKTWISGNLEPAGRLTVDAGAVKALKSGKSLLP
CCCCCHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCC
AGVKEVDGDFERGDTVAVMNEDGREIARGLIAYDAADARKVAGHKSDEISAILGYDARAA
CCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCCEEE
MIHRNDLVVRAASDAKAA
EEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA