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The map label for this gene is ispG [H]

Identifier: 163845355

GI number: 163845355

Start: 1214407

End: 1215669

Strand: Reverse

Name: ispG [H]

Synonym: BSUIS_B1254

Alternate gene names: 163845355

Gene position: 1215669-1214407 (Counterclockwise)

Preceding gene: 163845357

Following gene: 163845353

Centisome position: 86.78

GC content: 60.1

Gene sequence:

>1263_bases
ATGTCGTCCGAGACCGTCAGCTATTTTTCGCATCCATTTCCCCGCCGCCAGTCGGTTGGCGTCAGTGTCGGTGGCGTTAT
CGTCGGCGGCAGTGCGCCGGTGGTGGTCCAGTCCATGACCAACACCGACACGGCCGATGTGGATTCCACGGTTGCACAGG
TGGTCGCGCTTCATCGTGCCGGTTCGGAAATCGTGCGCATCACGGTGGATCGTGATGAATCGGCGGCTGCCGTGCCGAAA
ATCCGCGAGCGGCTGGAGCGTCTTGGCCATGATGTGCCGCTGGTGGGCGATTTCCATTATATCGGTCACAAGCTGCTTGC
CGATCATCCGGCCTGTGCGGAGGCTCTTGCCAAATACCGCATCAATCCCGGCAATGTCGGCTTCAAGGACAAGAAGGACA
AGCAGTTCGCCGATATTGTCGAAATGGCGATCCGCTACGACAAGCCCGTGCGCATCGGCGTCAACTGGGGATCGCTCGAT
CAGGAGCTTTTGACCACGCTGATGGACCGCAATCAGGCCGAGGGTGCGCCGCTTTCCGCGCAGGATGTCATGCGTGAAGC
CATTGTGCAGTCTGCGCTGATTTCGGCCAATCTTGCCGAGGAAATCGGGCTTGGCCGCGACAAGATCATCCTGTCGGCCA
AGGTCAGCCAGGTGCAGGATCTGATTGCCGTCTATACGATGCTGGCGCAGCGTTCCAATCATGCGCTGCATCTGGGACTT
ACCGAAGCGGGGATGGGCACGAAGGGCATTGTCGCGTCGTCGGCTGCCATGGGCATTTTGTTGCAGCAGGGCATTGGCGA
TACGATCCGCATTTCCCTGACGCCGGAACCGGGCGGCGACCGCACGCGTGAAGTGCAGGTGGCGCAGGAATTGCTGCAAA
CCATGGGGTTCCGCCAGTTTGTTCCGATTGTCGCCGCCTGTCCGGGCTGCGGCCGCACGACGTCCACGGTGTTTCAGGAA
CTGGCGCAGACCATTCAGGAAGATATCCGCCGCAATATGCCGCTCTGGCGCGAGAAATATCCGGGTGTCGAGGCGCTTTC
GGTTGCTGTCATGGGCTGTATCGTCAATGGGCCGGGCGAATCGAAACATGCCGATATCGGCATTTCGCTTCCGGGCACCG
GCGAAACCCCGTCCGCCCCGGTTTTTGTGGATGGCAAGAAGGTCGCGACCCTGCGTGGTCCGGGGATTGCCGAGGACTTC
CAGAAGATGGTTGCCGACTATATCGAAAACCGCTTTGGGCTTGGTCGTAAGATCGCGTCGTGA

Upstream 100 bases:

>100_bases
AAGCGAAAGCCGGATATACGCAGACCCGGATCTATGCCAGCGTTGACATTTGTGTCGCGCTACGCCACCTCAAAACGAGT
ACAGTCAAGAACAGAGCAAA

Downstream 100 bases:

>100_bases
GACCTTGTTAATTCTGCCGCTCGGCGATGAAGCGGGCCGCTTGCTTGAGGGGTTCGGCGTCTTCTCCAAAGGGTGCGAGC
AGGCTTTCGGCCTGTGCGAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVVALHRAGSEIVRITVDRDESAAAVPK
IRERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLD
QELLTTLMDRNQAEGAPLSAQDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGL
TEAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQE
LAQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFVDGKKVATLRGPGIAEDF
QKMVADYIENRFGLGRKIAS

Sequences:

>Translated_420_residues
MSSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVVALHRAGSEIVRITVDRDESAAAVPK
IRERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLD
QELLTTLMDRNQAEGAPLSAQDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGL
TEAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQE
LAQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFVDGKKVATLRGPGIAEDF
QKMVADYIENRFGLGRKIAS
>Mature_419_residues
SSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVVALHRAGSEIVRITVDRDESAAAVPKI
RERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLDQ
ELLTTLMDRNQAEGAPLSAQDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGLT
EAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQEL
AQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFVDGKKVATLRGPGIAEDFQ
KMVADYIENRFGLGRKIAS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=380, Percent_Identity=33.4210526315789, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45058; Mature: 44927

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVVALHRA
CCCCCCHHHCCCCCCCCCCCEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
GSEIVRITVDRDESAAAVPKIRERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYR
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHC
INPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNQAEGAPLSA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCH
QDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TEAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQF
HHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHH
VPIVAACPGCGRTTSTVFQELAQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECCCCC
SKHADIGISLPGTGETPSAPVFVDGKKVATLRGPGIAEDFQKMVADYIENRFGLGRKIAS
CCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVVALHRA
CCCCCHHHCCCCCCCCCCCEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
GSEIVRITVDRDESAAAVPKIRERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYR
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHC
INPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNQAEGAPLSA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCH
QDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TEAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQF
HHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHH
VPIVAACPGCGRTTSTVFQELAQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECCCCC
SKHADIGISLPGTGETPSAPVFVDGKKVATLRGPGIAEDFQKMVADYIENRFGLGRKIAS
CCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA