Click here to switch to the map view.

The map label for this gene is rpoH2 [H]

Identifier: 163845340

GI number: 163845340

Start: 1203031

End: 1203966

Strand: Reverse

Name: rpoH2 [H]

Synonym: BSUIS_B1239

Alternate gene names: 163845340

Gene position: 1203966-1203031 (Counterclockwise)

Preceding gene: 163845342

Following gene: 163845334

Centisome position: 85.95

GC content: 58.55

Gene sequence:

>936_bases
ATGAAGCCGTCATCCATGAGCAGTTCTTCCACAGTTCCAGCCAATCGTATGCCTATGGCGGTTCGCAGTTTCCCCGCAGC
CGCCGCTTCCTCCCAGTCCATGATCCGCAGCGCAATGTCCGCGCCCTATCTGGAGCGCGAGGAAGAGCGCGCGCTCGCCA
TCCGCTGGAAGGAGACGAAGGATCAGGATGCACTGCATCGGATTACATCCGCCCATATGCGGCTGGTCACTTCCATGGCC
GGCCGTTTTCGCAAGTTCAAGCTGCCGATGAGTGATCTCATACAGGAGGGCTATGTCGGGCTTCTGGAAGCGGCGGCGCG
TTTCGATCCCGACCGCGATGTGCGTTTTTCGACCTATGCATCCTGGTGGATTCGTGCCTCCATGCAGGATTATATCCTGC
GCAACTGGTCTATCGTTCGTGGTGGCACAAGCTCGGCGCAGAAAGCACTGTTTTTCAACCTGTGCCGCCTGCGTTCCAAG
CTCGCGCAGACTGACGACAACATGACCAAAGCCGAAATTTATCGGGAGATTTCCGACCAACTTGGTGTTTCTACGAGCGA
TGTCGAGTTCATGGACAGCCGCCTCTCGGGGCCGGACAGTTCGTTATCCGCCCCGATGAACGGCGAGGAGACCGGCGCTT
CCCGGCGTATGGATTTTCTGGTGGACGACCATCCCTTGCAGGATGAGATCGCCGAAAGCTCTATCGATATGGAGCGCCGC
AACTGCTGGCTCACACGCGCGCTCGACACGTTGAACGAGCGCGAATTGCACATTATTCGCGAGCGCCGCCTGGATGATGA
TGGTGTAACGCTGGAGGCGCTGGGCGAGAAGCTTGGCATTTCCAAGGAGCGGGTCCGCCAGATCGAGAGCCGGGCGATGG
AAAAGCTGAAGATCGCGCTTCTCAGCCAGCATGAGCTTGCCGCCTATCAGGCCTGA

Upstream 100 bases:

>100_bases
AAAGAGTTTGGGGCAATATGTTGCCTGGGGCATTCTTTTGGGACTGGCATATCCTTCCACTTAGCGTCCGGAGGGCGTGA
GTCCAGAACAGGAGAACCAG

Downstream 100 bases:

>100_bases
GCCGCCGAAAGGCGGCAGGCGCTGAACGGGGGCAGAAAGGCTCCATGTTCTTACGCATTATCCGATGCATCGAAGCGGGA
TTGGAAATCAATCCGGTGAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKPSSMSSSSTVPANRMPMAVRSFPAAAASSQSMIRSAMSAPYLEREEERALAIRWKETKDQDALHRITSAHMRLVTSMA
GRFRKFKLPMSDLIQEGYVGLLEAAARFDPDRDVRFSTYASWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLCRLRSK
LAQTDDNMTKAEIYREISDQLGVSTSDVEFMDSRLSGPDSSLSAPMNGEETGASRRMDFLVDDHPLQDEIAESSIDMERR
NCWLTRALDTLNERELHIIRERRLDDDGVTLEALGEKLGISKERVRQIESRAMEKLKIALLSQHELAAYQA

Sequences:

>Translated_311_residues
MKPSSMSSSSTVPANRMPMAVRSFPAAAASSQSMIRSAMSAPYLEREEERALAIRWKETKDQDALHRITSAHMRLVTSMA
GRFRKFKLPMSDLIQEGYVGLLEAAARFDPDRDVRFSTYASWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLCRLRSK
LAQTDDNMTKAEIYREISDQLGVSTSDVEFMDSRLSGPDSSLSAPMNGEETGASRRMDFLVDDHPLQDEIAESSIDMERR
NCWLTRALDTLNERELHIIRERRLDDDGVTLEALGEKLGISKERVRQIESRAMEKLKIALLSQHELAAYQA
>Mature_311_residues
MKPSSMSSSSTVPANRMPMAVRSFPAAAASSQSMIRSAMSAPYLEREEERALAIRWKETKDQDALHRITSAHMRLVTSMA
GRFRKFKLPMSDLIQEGYVGLLEAAARFDPDRDVRFSTYASWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLCRLRSK
LAQTDDNMTKAEIYREISDQLGVSTSDVEFMDSRLSGPDSSLSAPMNGEETGASRRMDFLVDDHPLQDEIAESSIDMERR
NCWLTRALDTLNERELHIIRERRLDDDGVTLEALGEKLGISKERVRQIESRAMEKLKIALLSQHELAAYQA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=288, Percent_Identity=35.4166666666667, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789098, Length=265, Percent_Identity=30.9433962264151, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=28.099173553719, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35294; Mature: 35294

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSSMSSSSTVPANRMPMAVRSFPAAAASSQSMIRSAMSAPYLEREEERALAIRWKETK
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
DQDALHRITSAHMRLVTSMAGRFRKFKLPMSDLIQEGYVGLLEAAARFDPDRDVRFSTYA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLCRLRSKLAQTDDNMTKAEIYREISDQ
HHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGVSTSDVEFMDSRLSGPDSSLSAPMNGEETGASRRMDFLVDDHPLQDEIAESSIDMERR
HCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCHHHEECCCCCHHHHHHHHCCHHHH
NCWLTRALDTLNERELHIIRERRLDDDGVTLEALGEKLGISKERVRQIESRAMEKLKIAL
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LSQHELAAYQA
HHHHHHHHCCC
>Mature Secondary Structure
MKPSSMSSSSTVPANRMPMAVRSFPAAAASSQSMIRSAMSAPYLEREEERALAIRWKETK
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
DQDALHRITSAHMRLVTSMAGRFRKFKLPMSDLIQEGYVGLLEAAARFDPDRDVRFSTYA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLCRLRSKLAQTDDNMTKAEIYREISDQ
HHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGVSTSDVEFMDSRLSGPDSSLSAPMNGEETGASRRMDFLVDDHPLQDEIAESSIDMERR
HCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCHHHEECCCCCHHHHHHHHCCHHHH
NCWLTRALDTLNERELHIIRERRLDDDGVTLEALGEKLGISKERVRQIESRAMEKLKIAL
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LSQHELAAYQA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]