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The map label for this gene is tolB [H]

Identifier: 163845276

GI number: 163845276

Start: 1148220

End: 1149551

Strand: Reverse

Name: tolB [H]

Synonym: BSUIS_B1172

Alternate gene names: 163845276

Gene position: 1149551-1148220 (Counterclockwise)

Preceding gene: 163845277

Following gene: 163845275

Centisome position: 82.06

GC content: 59.31

Gene sequence:

>1332_bases
ATGAAAATCGGCATCATCAATACAAAGATCCGGACGGTCTTTTCGGCTTTCGCGTGTATGATTGCCGCAAGCCTCGTCTG
CACCATGCCGGCGCGCGCTGTCGTGGAAATCAACATCAACAAGGGTGTGATCGAGCCGCTGCCGATCGCCATCACCGATT
TTCTTTCGGCTGATCAGCTTGGATCGAATATCACGTCGGTCATTGCGGCCGATCTTGAGCGGTCCGGCCTCTTCGCCCCC
ATAGACAAGGGCGCTTTCATCGAAAAGATCTCCAATCCGGATGCGGCGCCGCGTTTCGAGGACTGGAAGGTCATCAATGC
GCAGGCGCTCGTTACGGGCCGCATCACCAAACAGCCCGATGGCAGGCTCAAGGCTGAGTTCCATCTCTGGGATACGTTTG
GCGGCCAGCAGATGATCGGCCAGCAATTTTTCACCACGCCGGATAACTGGCGTCGTGTTGCCCATATCATCGCGGACGCC
ATCTATGAGCGCCTGACCGGTGACAAAGGCTATTTCGATACCCGTGTCGTTTTCGTGGATGAATCAGGCCCGGCGCAGAA
GCGTGTGAAGCGCCTTGCCATCATGGACCAGGACGGCGCCAATGTGCGTTTTATTTCTGATGGCCGCGCCCTTTCGCTGA
CGCCGCGCTTCTCGCCGAACCGGCAGGAAGTGACCTATATGTCGTTTGAGGGCGGCTCGCCGAAGGTCTATCTGCTGCAA
CTTGAAACCGGCCAGCGCGAGCTTGTCGGCAACTTCCCCGGCATGACTATTGCGCCGCGTTTTTCGCCCGACGGCCAGAA
GGTCGTGATGAGCCTGCTGCAGGATGACGGCAGCGCCAATATCTATACGATGGATCTGCGCAATCGCACGACCACGCGCC
TGACCAGCAGCCAGGCGATCGATACGGGCGCTTCCTATTCGCCGGATGGTTCGCAGATTGTCTTCACATCCGACCGTGGC
GGGCGCCCGCAGCTCTATGTCATGGGTGCCGATGGCTCCAATCCGCGCCGTATCTCGATGGGTGACGGCAGCTATTCCAC
GCCGGTCTGGTCCCCGCGCGGCGATCTCATCGCTTTCACCAAGCAATCGCAGGGCCAGTTCTCCATTGGTGTGACGAAGA
CCGATGGTTCGGGTGAGCGTCTGCTGACATCGGGCTTTCACAATGAAGGCCCGACATGGGCCCCGAATGGCCGCGTGCTG
ATGTTTTTCCGCAAGGCAGCCGGTGCAGGCGGCCCGAAACTCTTCACCATCGACCTGACCGGCCGTAACGAACGCCAGAT
CCAGACGCCGAATTTTGCTTCCGACCCGGCCTGGTCGCCGCTGCTCGAATAG

Upstream 100 bases:

>100_bases
TGGTCGGAAGTGATCGTCAATTTCGACCCAAGCGAAATGTTCTGACCGGGGTTCCGGTCGCCAACCCATCACTGCCAAGG
ACAAAGAGGTCCCAGAAGAC

Downstream 100 bases:

>100_bases
CGATCATCGAAAAAATGACAGGGTTGAAGGCGTGAAGCTTCGGTTTCGCGCCTTTATTTATGGCTGATTGCCACAATCAG
GGGGGCAAAGCCGTGTTTCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFHLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVTKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE

Sequences:

>Translated_443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFHLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVTKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE
>Mature_443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFHLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVTKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=434, Percent_Identity=30.4147465437788, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48480; Mature: 48480

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCHHHH
GSNITSVIAADLERSGLFAPIDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPD
CCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
GRLKAEFHLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADAIYERLTGDKGYFDTRVVFVD
CCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
CCCCHHHHHHHHEEECCCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEE
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAI
ECCCCHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCC
DTGASYSPDGSQIVFTSDRGGRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFT
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KQSQGQFSIGVTKTDGSGERLLTSGFHNEGPTWAPNGRVLMFFRKAAGAGGPKLFTIDLT
ECCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
GRNERQIQTPNFASDPAWSPLLE
CCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCHHHH
GSNITSVIAADLERSGLFAPIDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPD
CCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
GRLKAEFHLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADAIYERLTGDKGYFDTRVVFVD
CCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
CCCCHHHHHHHHEEECCCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEE
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAI
ECCCCHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCC
DTGASYSPDGSQIVFTSDRGGRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFT
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KQSQGQFSIGVTKTDGSGERLLTSGFHNEGPTWAPNGRVLMFFRKAAGAGGPKLFTIDLT
ECCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
GRNERQIQTPNFASDPAWSPLLE
CCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA