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The map label for this gene is gsiB [H]

Identifier: 163845195

GI number: 163845195

Start: 1055232

End: 1056851

Strand: Reverse

Name: gsiB [H]

Synonym: BSUIS_B1080

Alternate gene names: 163845195

Gene position: 1056851-1055232 (Counterclockwise)

Preceding gene: 163845196

Following gene: 163845192

Centisome position: 75.44

GC content: 52.9

Gene sequence:

>1620_bases
ATGCTATTCAAGACAACGGGAACAAGGTCTGGATCATTGAAAACATTGCGAAGCCTGATGATGGCGGCAACCTTGCTGAC
GGCATTCTCGGGGATTGCCTCGGCACAAAGTGCCAATTCACTGGTTATCGCGCGAAATATGGATATCAACTCGCTCGATC
CGCACCGGACCTTCTGCGATACCTGCCAGATCTACGATTCTGCCGTCTATGAGGGTTTGCTTTCGCTGGATAAGGACAAC
AAGGTTGTGCCAGTGCTTGCCGAAAGCTATGCAGCCAATGGCGACCAGACCGAGTTTACGTTCAAAATCAATCCAAAGGC
CGTATTCTCGGATGGTTCACGGGTGGAAGCAAAAGACGTCAAATGGTCGTGGGAACGGCTTAAAAACGTCAAGGGCAGCC
CGTCGTTCCTGGCCGATACTATTGCTTCGATAGAAACGCCCGACGAAGCAACAGTCGTGGTCAAAACCTGGGCACCGAAT
TCTGAATTCTTCAATGTTGTAGCCTCGCCTTATTTCGGTATCGTGAACAGCGATGTGGCCACGACCGAGGCTAAGGCGGA
AAATGGGGCGGACGCAGCGGAGAAAGATAATGCGGAAGCGTGGTTTCTCGCGCATTCGGCTGGCAGCGGTCCCTTCGTCC
TCAGCGCTTATGAGCCAAATTCTGAACTGCGTCTAAAGCGAAACGAAAAATACTGGCGGGAAGCGCCGAAGGTGGATGAG
GTCATCTTCCGCCAAGTGAAGGACGCTGTGGCACAGGCACAAATGCTGGAGAATGGCACTGCAGACATCGCGATGCAGAT
TGATCCCGAGACGGCCAAGACATTCCGTAACAAGGACATCAAGGTCGATTCCATCCATTCCTACAACTTCATCTATCTGG
CGCTTTCGCCGGGTGCAAAATCGAACGCCGTGCCGCTGACTGCAGACATTCGAGAGGCCATTTCTATTGCGATCGACCGT
GAATCGCTGCTCGACTTCATGCTTGGAGACAAAGGTCAGTTGATCGGAACGGCAATTCCGATAGGTTTTCCGGGCGGAAC
GGGGCATAAAATTCCCGAATATAATCCCGAAAAAGCCAAGGAGCTGCTTGCCAAGGCCGGTCATCCTAATGGTTTCACGC
TGGAAGCCGTTTATCCGAACCTGAACGTCTATGGCGTAGATTTCACCCAGATGATGCAAAAGATCCAGCAGGATCTTTCT
CAGATCAATGTCAAGGTCGAGCTGCAGCCGGTGCCGTTTGCAAGCTGGCGTGAAAAGGCTAATGGCGACGGAATCCCGAT
GACAGCCGTTTATTACGCGCCGGATTTCTTCGGCACATCCCAGTATGTGGATGCATTTGGATTGGCGAAGGGCAGTCTCT
GGGCAAAACGCGCCGGGGAGGCTCGCGATCCATCATTCGTAAAGACTGAAATTCCGCAGATCATGGCAGATGCCCTCAGA
GCACCGCTAGATGAGGCGGACAAGGTCTGGTTCAAGGCGGGCGAGGCCATGGCCGCGTCGAATGTTATCATTCCGATGCT
GAGCCCAGACGTTATTCTGGCATATAACAACAGGGTGAACGGCGTGCGCTATTCCGCCTGCTGCAATCTGCCATTGCAGG
AGCTTTCCGTATCCAGATAG

Upstream 100 bases:

>100_bases
TAAACAAGGCCGCCCTCGGATCGGCCGACAAGGTCCGGTCGGCCCTTGAGCAGATTACGCATTCCGCATGACAAAAAACG
TAAAATACGAGGAGGACAAA

Downstream 100 bases:

>100_bases
GCATGACGGGAACCTGTTTTTGAAAAGAAAAAGCCACCGATCCCGCATCGGTGGCTTTTTGCTATGGCAGGCTTTTGTGT
CGCTCAGCCTGCCGCGGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTWAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR

Sequences:

>Translated_539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTWAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
>Mature_539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTWAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=396, Percent_Identity=28.7878787878788, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787762, Length=433, Percent_Identity=26.0969976905312, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789887, Length=416, Percent_Identity=25.7211538461538, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789966, Length=446, Percent_Identity=23.3183856502242, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787551, Length=431, Percent_Identity=22.737819025522, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58906; Mature: 58906

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCD
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHH
TCQIYDSAVYEGLLSLDKDNKVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDV
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECCEEEECCCCEEECCCC
KWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTWAPNSEFFNVVASPYFGIVNSDVA
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEECCCCC
TTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHCCCHHH
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAK
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEEEECCEEEEEEECCCCC
SNAVPLTADIREAISIAIDRESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAK
CCCCEEHHHHHHHHHEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLSQINVKVELQPVPFASWREKA
HHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCC
NGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
CCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHHCCCC
>Mature Secondary Structure
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCD
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHH
TCQIYDSAVYEGLLSLDKDNKVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDV
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECCEEEECCCCEEECCCC
KWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTWAPNSEFFNVVASPYFGIVNSDVA
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEECCCCC
TTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHCCCHHH
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAK
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEEEECCEEEEEEECCCCC
SNAVPLTADIREAISIAIDRESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAK
CCCCEEHHHHHHHHHEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLSQINVKVELQPVPFASWREKA
HHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCC
NGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
CCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA