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The map label for this gene is lepA [H]

Identifier: 163845152

GI number: 163845152

Start: 1012826

End: 1014634

Strand: Reverse

Name: lepA [H]

Synonym: BSUIS_B1034

Alternate gene names: 163845152

Gene position: 1014634-1012826 (Counterclockwise)

Preceding gene: 163845153

Following gene: 163845150

Centisome position: 72.43

GC content: 57.55

Gene sequence:

>1809_bases
ATGAGCACACCACTTGACCATATTCGCAATTTTTCGATCGTCGCCCATATCGACCACGGCAAATCGACGCTGGCCGACCG
CCTCATTCAATTGACCGGCGGGCTGGACACGCGCGAGATGAAGGATCAGGTCCTCGACTCGATGGATATCGAGCGCGAGC
GCGGCATCACCATCAAGGCGCAGACCGTTCGTCTCAGCTACAAGGCAAAGAACGGCGAAGATTATGTGCTGAACCTGATC
GACACACCCGGCCACGTTGACTTCGCCTATGAAGTCTCGCGTTCGCTGGCCGCATGTGAAGGTTCGCTTCTGGTGGTGGA
TGCGTCGCAGGGCGTGGAAGCGCAGACGCTGGCCAATGTCTATCAGGCCATCGACAACAACCACGAAATCGTGGTCGTGC
TGAACAAGATCGATTTGCCTGCCGCCGAACCTGAGCGCGTCAAGCAACAGATCGAGGAAGTGATCGGCATTGACGCCTCC
GACGCGGTGGAGATTTCCGCCAAGACCGGCCTCGGTATCGAGGACGTGTTGGAAGCTATCGTCAACAAGCTGCCTGCGCC
GAAGGAAGGCGACCGCAATGCCCCGCTGAAAGCCATGCTGGTGGATAGCTGGTATGATTCCTATCTCGGCGTTATCGTTC
TGGTGCGCGTCATCGATGGTGTGCTGAAAAAGGGTCAGACCATCCGCATGATGGGCACGGGCGCGAAATATCCGGTGGAA
CGCACCGGTGTCTTTACGCCCAAGATGGTTCAGGTGGACGATCTTGGTCCCGGTGAGCTTGGCTTCATCACCGCTTCCAT
CAAGGAAGTGGCCGATACCCGCGTCGGTGATACGATTACCGAGGATCGCCGCCCCACGGAAAACATGCTGTCCGGCTTCA
AGCCTGCGCAGCCGGTTGTATTCTGCGGCCTGTTCCCGGTCGATGCGGCGGATTTTGAAGATCTGCGCGGCGCCATGGGC
AAGCTGCGCCTCAACGATGCGTCGTTCTCGTTTGAAATGGAAACGTCTGCCGCGCTCGGCTTTGGTTTCCGTTGCGGGTT
CCTTGGCCTTCTGCATCTGGAAATCATTCAGGAACGTCTTGAACGCGAGTTCAATCTTGATCTTATCACCACCGCGCCTT
CGGTTGTCTATCGCCTCAACATGACGGACGGGACGCACAAGGAACTGCACAACCCGGCTGATATGCCGGATGTGGTGAAG
ATTGCCTCCATCGAGGAGCCGTGGATCAAGGCGACCATCATGACGCCGGATGATTATCTCGGCGCGATCATGAAGCTGTG
TCAGGAGCGCCGCGGCATCCAGATCGATCTCACCTATGTCGGCCCACGCGCCATGATTACCTATGATCTGCCGCTCAACG
AAGTGGTGTTCGATTTCTACGACCGCCTGAAGTCGATCTCCAAGGGCTATGCCTCGTTCGACTATAATCTTTCGGATTAT
CGCGAGGGCGATCTGGTCAAGATGTCGATCCTCGTCAACGAAGAGCCGGTCGATGCGCTGTCGATGCTGGTGCACCGCTC
GGCTGCTGAAAAGCGTGGCCGTGCGCTGTGCGAAAAGCTCAAGGAACTGATCCCGCAACATATGTTCAAGATTCCGATCC
AGGCGGCCATCGGTGGTCGCATCGTGGCTCGCGAGACGATCTCGGCGCTGCGCAAGGACGTGACGGCGAAGTGCTACGGC
GGCGACGTGACGCGCAAGCGCAAACTTCTGGAAAAGCAGAAGGAAGGCAAGAAGCGCATGCGCCAGTTCGGCAAGGTCGA
AATCCCGCAGGAGGCCTTCATTCAGGCACTCAAGATGGGCGATGATTGA

Upstream 100 bases:

>100_bases
AAAGGGGACTTGTTGTCACGATTGCGAAAGCTGCCGGTTTTCCCGCATTTTGGCGCGAATACTCCTTTGAAATTTGGTGG
CTTGCTGTTAAATCGCGCGC

Downstream 100 bases:

>100_bases
TCGCTTGGTGATCTGTTCAATATTGTCTTGGATTGGGCGCTTCGGCGCCCATTCTTTTTGAACTTTGAAAACGCCAATCT
CGTCCTCATCCTGAGGAGCC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQLTGGLDTREMKDQVLDSMDIERERGITIKAQTVRLSYKAKNGEDYVLNLI
DTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKIDLPAAEPERVKQQIEEVIGIDAS
DAVEISAKTGLGIEDVLEAIVNKLPAPKEGDRNAPLKAMLVDSWYDSYLGVIVLVRVIDGVLKKGQTIRMMGTGAKYPVE
RTGVFTPKMVQVDDLGPGELGFITASIKEVADTRVGDTITEDRRPTENMLSGFKPAQPVVFCGLFPVDAADFEDLRGAMG
KLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLITTAPSVVYRLNMTDGTHKELHNPADMPDVVK
IASIEEPWIKATIMTPDDYLGAIMKLCQERRGIQIDLTYVGPRAMITYDLPLNEVVFDFYDRLKSISKGYASFDYNLSDY
REGDLVKMSILVNEEPVDALSMLVHRSAAEKRGRALCEKLKELIPQHMFKIPIQAAIGGRIVARETISALRKDVTAKCYG
GDVTRKRKLLEKQKEGKKRMRQFGKVEIPQEAFIQALKMGDD

Sequences:

>Translated_602_residues
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQLTGGLDTREMKDQVLDSMDIERERGITIKAQTVRLSYKAKNGEDYVLNLI
DTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKIDLPAAEPERVKQQIEEVIGIDAS
DAVEISAKTGLGIEDVLEAIVNKLPAPKEGDRNAPLKAMLVDSWYDSYLGVIVLVRVIDGVLKKGQTIRMMGTGAKYPVE
RTGVFTPKMVQVDDLGPGELGFITASIKEVADTRVGDTITEDRRPTENMLSGFKPAQPVVFCGLFPVDAADFEDLRGAMG
KLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLITTAPSVVYRLNMTDGTHKELHNPADMPDVVK
IASIEEPWIKATIMTPDDYLGAIMKLCQERRGIQIDLTYVGPRAMITYDLPLNEVVFDFYDRLKSISKGYASFDYNLSDY
REGDLVKMSILVNEEPVDALSMLVHRSAAEKRGRALCEKLKELIPQHMFKIPIQAAIGGRIVARETISALRKDVTAKCYG
GDVTRKRKLLEKQKEGKKRMRQFGKVEIPQEAFIQALKMGDD
>Mature_601_residues
STPLDHIRNFSIVAHIDHGKSTLADRLIQLTGGLDTREMKDQVLDSMDIERERGITIKAQTVRLSYKAKNGEDYVLNLID
TPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNNHEIVVVLNKIDLPAAEPERVKQQIEEVIGIDASD
AVEISAKTGLGIEDVLEAIVNKLPAPKEGDRNAPLKAMLVDSWYDSYLGVIVLVRVIDGVLKKGQTIRMMGTGAKYPVER
TGVFTPKMVQVDDLGPGELGFITASIKEVADTRVGDTITEDRRPTENMLSGFKPAQPVVFCGLFPVDAADFEDLRGAMGK
LRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLITTAPSVVYRLNMTDGTHKELHNPADMPDVVKI
ASIEEPWIKATIMTPDDYLGAIMKLCQERRGIQIDLTYVGPRAMITYDLPLNEVVFDFYDRLKSISKGYASFDYNLSDYR
EGDLVKMSILVNEEPVDALSMLVHRSAAEKRGRALCEKLKELIPQHMFKIPIQAAIGGRIVARETISALRKDVTAKCYGG
DVTRKRKLLEKQKEGKKRMRQFGKVEIPQEAFIQALKMGDD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.3377483443709, Blast_Score=616, Evalue=1e-176,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=48.1203007518797, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI18390331, Length=185, Percent_Identity=36.2162162162162, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI25306283, Length=156, Percent_Identity=42.9487179487179, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.2777777777778, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=156, Percent_Identity=42.9487179487179, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=156, Percent_Identity=42.9487179487179, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=44.5454545454545, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=44.5454545454545, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=44.5454545454545, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=37.3134328358209, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=37.3134328358209, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=42.2680412371134, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729339, Length=223, Percent_Identity=28.6995515695067, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI53729337, Length=223, Percent_Identity=28.6995515695067, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=57.8595317725752, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=523, Percent_Identity=28.2982791586998, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1789738, Length=186, Percent_Identity=35.4838709677419, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=31.6770186335404, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=235, Percent_Identity=29.3617021276596, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=616, Percent_Identity=39.6103896103896, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17556745, Length=194, Percent_Identity=35.5670103092784, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=39.8550724637681, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=39.8550724637681, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=38.7755102040816, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=35.8974358974359, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=229, Percent_Identity=31.0043668122271, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566303, Length=252, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=250, Percent_Identity=28.4, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=45.364238410596, Blast_Score=544, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=35.9375, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=39.5833333333333, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=39.5833333333333, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=139, Percent_Identity=38.1294964028777, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=35.8823529411765, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=244, Percent_Identity=29.0983606557377, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=45.1986754966887, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=39.8773006134969, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=43.7956204379562, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=35.8108108108108, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=35.8108108108108, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=35.8108108108108, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357743, Length=169, Percent_Identity=34.9112426035503, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221458488, Length=142, Percent_Identity=40.8450704225352, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=226, Percent_Identity=30.5309734513274, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66896; Mature: 66765

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLDHIRNFSIVAHIDHGKSTLADRLIQLTGGLDTREMKDQVLDSMDIERERGITIKA
CCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QTVRLSYKAKNGEDYVLNLIDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANV
EEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YQAIDNNHEIVVVLNKIDLPAAEPERVKQQIEEVIGIDASDAVEISAKTGLGIEDVLEAI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
VNKLPAPKEGDRNAPLKAMLVDSWYDSYLGVIVLVRVIDGVLKKGQTIRMMGTGAKYPVE
HHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RTGVFTPKMVQVDDLGPGELGFITASIKEVADTRVGDTITEDRRPTENMLSGFKPAQPVV
CCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCCCEE
FCGLFPVDAADFEDLRGAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERL
EEEECCCCCCCHHHHHHHHCEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLITTAPSVVYRLNMTDGTHKELHNPADMPDVVKIASIEEPWIKATIMTPDDYL
HHHCCCEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEECCCCCCCEEEEEECCHHHH
GAIMKLCQERRGIQIDLTYVGPRAMITYDLPLNEVVFDFYDRLKSISKGYASFDYNLSDY
HHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCC
REGDLVKMSILVNEEPVDALSMLVHRSAAEKRGRALCEKLKELIPQHMFKIPIQAAIGGR
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
IVARETISALRKDVTAKCYGGDVTRKRKLLEKQKEGKKRMRQFGKVEIPQEAFIQALKMG
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DD
CC
>Mature Secondary Structure 
STPLDHIRNFSIVAHIDHGKSTLADRLIQLTGGLDTREMKDQVLDSMDIERERGITIKA
CCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QTVRLSYKAKNGEDYVLNLIDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANV
EEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YQAIDNNHEIVVVLNKIDLPAAEPERVKQQIEEVIGIDASDAVEISAKTGLGIEDVLEAI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
VNKLPAPKEGDRNAPLKAMLVDSWYDSYLGVIVLVRVIDGVLKKGQTIRMMGTGAKYPVE
HHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RTGVFTPKMVQVDDLGPGELGFITASIKEVADTRVGDTITEDRRPTENMLSGFKPAQPVV
CCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCCCEE
FCGLFPVDAADFEDLRGAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERL
EEEECCCCCCCHHHHHHHHCEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLITTAPSVVYRLNMTDGTHKELHNPADMPDVVKIASIEEPWIKATIMTPDDYL
HHHCCCEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEECCCCCCCEEEEEECCHHHH
GAIMKLCQERRGIQIDLTYVGPRAMITYDLPLNEVVFDFYDRLKSISKGYASFDYNLSDY
HHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCC
REGDLVKMSILVNEEPVDALSMLVHRSAAEKRGRALCEKLKELIPQHMFKIPIQAAIGGR
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
IVARETISALRKDVTAKCYGGDVTRKRKLLEKQKEGKKRMRQFGKVEIPQEAFIQALKMG
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA