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The map label for this gene is ycdT [H]

Identifier: 163845119

GI number: 163845119

Start: 980735

End: 981856

Strand: Reverse

Name: ycdT [H]

Synonym: BSUIS_B0999

Alternate gene names: 163845119

Gene position: 981856-980735 (Counterclockwise)

Preceding gene: 163845120

Following gene: 163845118

Centisome position: 70.09

GC content: 59.36

Gene sequence:

>1122_bases
GTGAAATTCGTTCCACCACTGGTTATCCTGCTTTTTGCTGCGACGATGCTGGGCGTCTGGATGCTTGACACGAAGCGCAA
GCATCTGCTGCTGTTTGGTCTGAGTTTCGTGGGCTTCGGCCTTGGTTTTCTTGCGCCGGTTTCCCAATTGTCGGATAGCC
TGGCGATAACGACGCCTATTGCAACCCTGTTCCATATTGCAGGCGCGCTGACCTTCTGCGAAGCCGTGATGGTGCGCATG
GGAAAACATTTCTCGCGGCTGGTTGGTGGAACGCTGGCGGTCGTCGTTTTCGGGGTTGTTATTGCCTTCATCTGCTTTGA
TGGCCGCTACGCCTTTATCGCGCCAACGGTTAATATGGGGCTGGGGCTTTTAACGGCGGCTTTGTGCGTGCAGGCGCTCG
CAGTCTTTTCTGCGCATTTTATCGTGCAGGCGGTGCTGGCTTTCTTCTTCGTTGGGGATGCCGCCGCCCCATCGGAACTT
GTCCATTCGCGCTATTGGCTGGGCCTCATGGTTGCCGTGTTGCTGGTGGGCGTTTTGGTCGGCTTGCTGATCCTCGTCGT
AGCGACGGCGGATGTGGTGAACGAACTCCAGCGCGAGCGGGATTCCGACCCGCTGACGGGGCTGTTGAACCGTCGCGGGC
TTGAGAGGCAGGCCGATCTTCGCCTGCACACACCGCAATGCCTCTCCTATGGCGTCATCACTGCCGATATCGATCATTTC
AAGGCGATCAACGATGCCTACGGCCATCCGGTGGGGGACCAGGTGCTGGTGGAGTTTGCCCGCATTCTTTCGTCCGCCAG
CAAGGACCATGCGATCGTGGGCCGCGTCGGCGGTGAGGAATTTGTGCTGCTGGTGAGCGGTACTGCCGAAGAATGCGAGG
CGCTGGGCAACCGGCTGTGCGGGCTGGTTGCGCGCCATGCCTTCTCTAAATTGCCGGCAGGACAGAAGGTCACATCCAGT
TTCGGCATCGCGATGGTGCGCAGCGGCGAGACATTCTGGGAAACGGCCGCTCGTGCCGACCTGGCCCTGATGCGGGTCAA
AAACCTTGGGCGCAACCGTGTTGCGGTGGAGGGGCTGGAATTCCCCAGCGCCATGCGGGGTACTTATCTTCTCACCGCAT
AA

Upstream 100 bases:

>100_bases
GCGGAGGAAATATTAGCTTGACTTTCACTTCAGTTTGTAATTGCACATAGGTTCGATCCTGGAATTTACGGTTTTATTTT
AAACGATTTAGGCTCTGTTT

Downstream 100 bases:

>100_bases
AACCGGGCTCTCTTGGGAATCTATGGGGCGCCCGATATTTCTCTTCACTTGACGCCATCCTGTAAAGGATTTGGTTACCA
TAATTAGCGATGCTTGGAGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKFVPPLVILLFAATMLGVWMLDTKRKHLLLFGLSFVGFGLGFLAPVSQLSDSLAITTPIATLFHIAGALTFCEAVMVRM
GKHFSRLVGGTLAVVVFGVVIAFICFDGRYAFIAPTVNMGLGLLTAALCVQALAVFSAHFIVQAVLAFFFVGDAAAPSEL
VHSRYWLGLMVAVLLVGVLVGLLILVVATADVVNELQRERDSDPLTGLLNRRGLERQADLRLHTPQCLSYGVITADIDHF
KAINDAYGHPVGDQVLVEFARILSSASKDHAIVGRVGGEEFVLLVSGTAEECEALGNRLCGLVARHAFSKLPAGQKVTSS
FGIAMVRSGETFWETAARADLALMRVKNLGRNRVAVEGLEFPSAMRGTYLLTA

Sequences:

>Translated_373_residues
MKFVPPLVILLFAATMLGVWMLDTKRKHLLLFGLSFVGFGLGFLAPVSQLSDSLAITTPIATLFHIAGALTFCEAVMVRM
GKHFSRLVGGTLAVVVFGVVIAFICFDGRYAFIAPTVNMGLGLLTAALCVQALAVFSAHFIVQAVLAFFFVGDAAAPSEL
VHSRYWLGLMVAVLLVGVLVGLLILVVATADVVNELQRERDSDPLTGLLNRRGLERQADLRLHTPQCLSYGVITADIDHF
KAINDAYGHPVGDQVLVEFARILSSASKDHAIVGRVGGEEFVLLVSGTAEECEALGNRLCGLVARHAFSKLPAGQKVTSS
FGIAMVRSGETFWETAARADLALMRVKNLGRNRVAVEGLEFPSAMRGTYLLTA
>Mature_373_residues
MKFVPPLVILLFAATMLGVWMLDTKRKHLLLFGLSFVGFGLGFLAPVSQLSDSLAITTPIATLFHIAGALTFCEAVMVRM
GKHFSRLVGGTLAVVVFGVVIAFICFDGRYAFIAPTVNMGLGLLTAALCVQALAVFSAHFIVQAVLAFFFVGDAAAPSEL
VHSRYWLGLMVAVLLVGVLVGLLILVVATADVVNELQRERDSDPLTGLLNRRGLERQADLRLHTPQCLSYGVITADIDHF
KAINDAYGHPVGDQVLVEFARILSSASKDHAIVGRVGGEEFVLLVSGTAEECEALGNRLCGLVARHAFSKLPAGQKVTSS
FGIAMVRSGETFWETAARADLALMRVKNLGRNRVAVEGLEFPSAMRGTYLLTA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=209, Percent_Identity=32.5358851674641, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=38.562091503268, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=39.2638036809816, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786584, Length=204, Percent_Identity=32.843137254902, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=30.2857142857143, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=34.7305389221557, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787802, Length=183, Percent_Identity=32.7868852459016, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=124, Percent_Identity=32.258064516129, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=33.1288343558282, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40033; Mature: 40033

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHCCCEEEEC
>Mature Secondary Structure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CCHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]