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The map label for this gene is pntB [H]

Identifier: 163845084

GI number: 163845084

Start: 946752

End: 948152

Strand: Reverse

Name: pntB [H]

Synonym: BSUIS_B0963

Alternate gene names: 163845084

Gene position: 948152-946752 (Counterclockwise)

Preceding gene: 163845085

Following gene: 163845082

Centisome position: 67.68

GC content: 59.31

Gene sequence:

>1401_bases
ATGAGCGTTAATCTCGCAGCCTTCCTTTATCTCGTCTCCGGCGTGCTGTTCATTCTGGCGCTGCGCGGCCTGTCGCATCC
GACAACCAGCCGTCAGGGCAATATGTACGGCATGATCGGCATGGCGATCTCCATCGTCACCACGCTTTTGCTGGCGCGCC
CGAGCTTCGGCGGTCTGGTGCTGATTATCGTCGGCATCGCTATTGGCGGCGGCATCGGTGCTGTCATTGCCCGCCGTATC
GCCATGACAGCCATGCCGCAGCTTGTCGCCGCCTTCCACTCACTGATCGGCCTTGCTGCCGTTCTGGTGGCTGCTGCCGC
ACTTTATTCGCCGCATTCCTTCGGGATTGGCGAAGTGGGCCAGATCCACGGTCAGGCGCTCATCGAAATGTCGCTCGGCG
TGGCCATTGGCGCAATCACCTTCACCGGCTCGATCATTGCCTTTCTGAAACTCGATGGCCGCATGTCGGGCAAGCCGATC
ATGCTGCCGGGCCGCCACGTCATCAATGCGGCTCTGGCCGCAGCCATCGTGCTTCTGATCGTCGTGCTGACCAACACGGA
AAGCCATTTCGTGTTCTGGCTGATCGTGCTTCTGTCGCTCGTCTTCGGCGTTCTTATCATCGTGCCCATTGGCGGCGCGG
ATATGCCGGTCGTCGTTTCCATGCTGAACTCCTATTCGGGTTGGGCGGCTGCTGGCATCGGCTTCACGCTTGGCAATCTG
GCGCTCATTATCACCGGCGCGCTGGTGGGTTCGTCAGGTGCGATCCTGTCCTACATCATGTGCAAAGGCATGAACCGTTC
GTTCATTTCGGTCATTCTCGGCGGCTTTGGCGGCGATACGTCTTCGGCTGCTGGCGGCGAGGTCGAGCAGCGCCCGGTCA
AGCAGGGTTCTGCCGACGATGCGGCCTTCATCCTGAAGAACGCTTCCAAGGTCATCATCGTGCCGGGCTATGGCATGGCG
GTGGCGCAGGCCCAGCATGCGCTGCGTGAAATGGCCGATAAACTGAAGGCTGAAGGCGTGGAAGTGAAATACGCCATCCA
CCCGGTCGCGGGCCGTATGCCGGGCCATATGAACGTGCTTCTGGCCGAGGCCAATGTGCCTTACGATGAAGTGTTCGAGC
TGGAAGACATCAATTCGGAATTCGCAACCGCCGATGTGGCCTTCGTGATTGGCGCCAACGACGTCACCAATCCGGCGGCG
AAGACTGATCCGAAGTCACCGATTTTCGGCATGCCGATCCTTGATGTGGACAAGGCCGGAACGGTCCTCTTCATCAAGCG
CGGCATGGGTTCCGGCTATGCGGGCGTGGAGAACGAACTCTTCTTCCGCGACAACACCATGATGCTCTTTGCCGATGCGA
AGAAGATGGTTGAGAGCATTGTGAAGGCGTTGGACCATTAA

Upstream 100 bases:

>100_bases
TGCGCTCATTCTGGCGAGCGTCAATATTTTCGGCGGCTTTCTGGTCACCCAGCGCATGCTTGCCATGTACAAGAAAAAAG
AAAAGTGAGGCTGAACCATT

Downstream 100 bases:

>100_bases
GGGAAGGCAGTAGGGCAGTATGTTTGATTTGCAAACATGTTCCCCCTATTCGCCTTACTCCCTTACTCCCTTACTCCCTT
ACTCCCTTAAAACATACTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSVNLAAFLYLVSGVLFILALRGLSHPTTSRQGNMYGMIGMAISIVTTLLLARPSFGGLVLIIVGIAIGGGIGAVIARRI
AMTAMPQLVAAFHSLIGLAAVLVAAAALYSPHSFGIGEVGQIHGQALIEMSLGVAIGAITFTGSIIAFLKLDGRMSGKPI
MLPGRHVINAALAAAIVLLIVVLTNTESHFVFWLIVLLSLVFGVLIIVPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNL
ALIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDTSSAAGGEVEQRPVKQGSADDAAFILKNASKVIIVPGYGMA
VAQAQHALREMADKLKAEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFATADVAFVIGANDVTNPAA
KTDPKSPIFGMPILDVDKAGTVLFIKRGMGSGYAGVENELFFRDNTMMLFADAKKMVESIVKALDH

Sequences:

>Translated_466_residues
MSVNLAAFLYLVSGVLFILALRGLSHPTTSRQGNMYGMIGMAISIVTTLLLARPSFGGLVLIIVGIAIGGGIGAVIARRI
AMTAMPQLVAAFHSLIGLAAVLVAAAALYSPHSFGIGEVGQIHGQALIEMSLGVAIGAITFTGSIIAFLKLDGRMSGKPI
MLPGRHVINAALAAAIVLLIVVLTNTESHFVFWLIVLLSLVFGVLIIVPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNL
ALIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDTSSAAGGEVEQRPVKQGSADDAAFILKNASKVIIVPGYGMA
VAQAQHALREMADKLKAEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFATADVAFVIGANDVTNPAA
KTDPKSPIFGMPILDVDKAGTVLFIKRGMGSGYAGVENELFFRDNTMMLFADAKKMVESIVKALDH
>Mature_465_residues
SVNLAAFLYLVSGVLFILALRGLSHPTTSRQGNMYGMIGMAISIVTTLLLARPSFGGLVLIIVGIAIGGGIGAVIARRIA
MTAMPQLVAAFHSLIGLAAVLVAAAALYSPHSFGIGEVGQIHGQALIEMSLGVAIGAITFTGSIIAFLKLDGRMSGKPIM
LPGRHVINAALAAAIVLLIVVLTNTESHFVFWLIVLLSLVFGVLIIVPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNLA
LIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDTSSAAGGEVEQRPVKQGSADDAAFILKNASKVIIVPGYGMAV
AQAQHALREMADKLKAEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFATADVAFVIGANDVTNPAAK
TDPKSPIFGMPILDVDKAGTVLFIKRGMGSGYAGVENELFFRDNTMMLFADAKKMVESIVKALDH

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=469, Percent_Identity=46.4818763326226, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI122939153, Length=469, Percent_Identity=46.4818763326226, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1787886, Length=469, Percent_Identity=55.863539445629, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17550456, Length=462, Percent_Identity=47.1861471861472, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48510; Mature: 48379

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNLAAFLYLVSGVLFILALRGLSHPTTSRQGNMYGMIGMAISIVTTLLLARPSFGGLV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
LIIVGIAIGGGIGAVIARRIAMTAMPQLVAAFHSLIGLAAVLVAAAALYSPHSFGIGEVG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
QIHGQALIEMSLGVAIGAITFTGSIIAFLKLDGRMSGKPIMLPGRHVINAALAAAIVLLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHH
VVLTNTESHFVFWLIVLLSLVFGVLIIVPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNL
HHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
ALIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDTSSAAGGEVEQRPVKQGSADD
HHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCC
AAFILKNASKVIIVPGYGMAVAQAQHALREMADKLKAEGVEVKYAIHPVAGRMPGHMNVL
HHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEE
LAEANVPYDEVFELEDINSEFATADVAFVIGANDVTNPAAKTDPKSPIFGMPILDVDKAG
EEECCCCHHHHHHHHHCCCCHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCC
TVLFIKRGMGSGYAGVENELFFRDNTMMLFADAKKMVESIVKALDH
CEEEEEECCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVNLAAFLYLVSGVLFILALRGLSHPTTSRQGNMYGMIGMAISIVTTLLLARPSFGGLV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
LIIVGIAIGGGIGAVIARRIAMTAMPQLVAAFHSLIGLAAVLVAAAALYSPHSFGIGEVG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
QIHGQALIEMSLGVAIGAITFTGSIIAFLKLDGRMSGKPIMLPGRHVINAALAAAIVLLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHH
VVLTNTESHFVFWLIVLLSLVFGVLIIVPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNL
HHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
ALIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDTSSAAGGEVEQRPVKQGSADD
HHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCC
AAFILKNASKVIIVPGYGMAVAQAQHALREMADKLKAEGVEVKYAIHPVAGRMPGHMNVL
HHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEE
LAEANVPYDEVFELEDINSEFATADVAFVIGANDVTNPAAKTDPKSPIFGMPILDVDKAG
EEECCCCHHHHHHHHHCCCCHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCC
TVLFIKRGMGSGYAGVENELFFRDNTMMLFADAKKMVESIVKALDH
CEEEEEECCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]