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The map label for this gene is yieH [H]

Identifier: 163845082

GI number: 163845082

Start: 944467

End: 945138

Strand: Reverse

Name: yieH [H]

Synonym: BSUIS_B0961

Alternate gene names: 163845082

Gene position: 945138-944467 (Counterclockwise)

Preceding gene: 163845084

Following gene: 163845081

Centisome position: 67.47

GC content: 59.23

Gene sequence:

>672_bases
ATGAACAGCGCATACCCATTCGACCTGTTTATTTTCGACTGTGATGGCGTGCTTGTCGATAGCGAGCCGCTTTCCTGCCG
GGCTTTCGAGCAGGTCTATGCTGATTATGGCATGGCGCTGCCGGAGGGGACGGTTGCGCAGGGCATCGGCATGAAACAGG
CTGATATCATGCAAATGATCGAGGATATGACCGGCTATCGCCTGCCGGAAGAGGCGGGCGCCGCATTTTGGCCCGCCACG
CGCACGCTGTTTGCGCAAGCCTTGCAGCCGACCGTCGGCATAGCGGACTTCCTGCGTGATTTGCCGCAAAAGCGTTGTGT
TGCTTCGTCGTCGCAACCCGAGCGCATTGCGTTCAGCCTTCAGAAGACGGGCCTCGACCAATATTTTGGCGATGCGGTCT
ATTCGTCCAGCATGGTCAAGCGCGGCAAGCCCGCGCCGGACCTGTTTCTGTTTGCGGCCGACAAGATGGGTGTCGATCCG
GCGCGTTGTGTGGTGATCGAGGATTCGCCCTTTGGCATTGAAGGCGCTGTTGCGGCAGGCATGACGGCTTTCGGCTATAC
GGGCGGCGGGCATAGCTATGATGGCCATGCCGAAAAACTGATGGCCAAGGGCGCGCGACAGGTTTTTGCGCATTGGGACG
AAATGGTCCGCGAAATTCTGGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
ACACTGACGGGCGCGCATCCATAAACTGCGCGACAAATTACGCATTCCCCCGAACGTGCGGCTCAAAAAGGAAGTCGAGG
GGTCTTCACCTGGAGTTACC

Downstream 100 bases:

>100_bases
GCGGCAAGGGCTTGCCGCTGCGTCTTTATCGGTTAAACAGAAGATGATATTATATCAGCAGGAAATGGCAGGGAATGAAA
AACGCGATCACGCTTGACCA

Product: HAD family hydrolase

Products: glycolate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNSAYPFDLFIFDCDGVLVDSEPLSCRAFEQVYADYGMALPEGTVAQGIGMKQADIMQMIEDMTGYRLPEEAGAAFWPAT
RTLFAQALQPTVGIADFLRDLPQKRCVASSSQPERIAFSLQKTGLDQYFGDAVYSSSMVKRGKPAPDLFLFAADKMGVDP
ARCVVIEDSPFGIEGAVAAGMTAFGYTGGGHSYDGHAEKLMAKGARQVFAHWDEMVREILAAA

Sequences:

>Translated_223_residues
MNSAYPFDLFIFDCDGVLVDSEPLSCRAFEQVYADYGMALPEGTVAQGIGMKQADIMQMIEDMTGYRLPEEAGAAFWPAT
RTLFAQALQPTVGIADFLRDLPQKRCVASSSQPERIAFSLQKTGLDQYFGDAVYSSSMVKRGKPAPDLFLFAADKMGVDP
ARCVVIEDSPFGIEGAVAAGMTAFGYTGGGHSYDGHAEKLMAKGARQVFAHWDEMVREILAAA
>Mature_223_residues
MNSAYPFDLFIFDCDGVLVDSEPLSCRAFEQVYADYGMALPEGTVAQGIGMKQADIMQMIEDMTGYRLPEEAGAAFWPAT
RTLFAQALQPTVGIADFLRDLPQKRCVASSSQPERIAFSLQKTGLDQYFGDAVYSSSMVKRGKPAPDLFLFAADKMGVDP
ARCVVIEDSPFGIEGAVAAGMTAFGYTGGGHSYDGHAEKLMAKGARQVFAHWDEMVREILAAA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=183, Percent_Identity=30.0546448087432, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789046, Length=177, Percent_Identity=34.4632768361582, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787576, Length=190, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137324, Length=183, Percent_Identity=35.5191256830601, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20129151, Length=192, Percent_Identity=30.7291666666667, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116008157, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18

Molecular weight: Translated: 24165; Mature: 24165

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAYPFDLFIFDCDGVLVDSEPLSCRAFEQVYADYGMALPEGTVAQGIGMKQADIMQMI
CCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
EDMTGYRLPEEAGAAFWPATRTLFAQALQPTVGIADFLRDLPQKRCVASSSQPERIAFSL
HHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QKTGLDQYFGDAVYSSSMVKRGKPAPDLFLFAADKMGVDPARCVVIEDSPFGIEGAVAAG
HHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHC
MTAFGYTGGGHSYDGHAEKLMAKGARQVFAHWDEMVREILAAA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSAYPFDLFIFDCDGVLVDSEPLSCRAFEQVYADYGMALPEGTVAQGIGMKQADIMQMI
CCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
EDMTGYRLPEEAGAAFWPATRTLFAQALQPTVGIADFLRDLPQKRCVASSSQPERIAFSL
HHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QKTGLDQYFGDAVYSSSMVKRGKPAPDLFLFAADKMGVDPARCVVIEDSPFGIEGAVAAG
HHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHC
MTAFGYTGGGHSYDGHAEKLMAKGARQVFAHWDEMVREILAAA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-phosphoglycolate; H2O

Specific reaction: 2-phosphoglycolate + H2O = glycolate + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]