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The map label for this gene is puuB [H]

Identifier: 163845022

GI number: 163845022

Start: 873963

End: 875246

Strand: Reverse

Name: puuB [H]

Synonym: BSUIS_B0898

Alternate gene names: 163845022

Gene position: 875246-873963 (Counterclockwise)

Preceding gene: 163845023

Following gene: 163845020

Centisome position: 62.48

GC content: 59.27

Gene sequence:

>1284_bases
ATGCTGAACGATCCGCGCTCCCATGGTCTTTGGGAAAAGACTGCTCCTGCCGCTCCCAAGACGACTGCGCTGCAAGGCGA
TCTGGCTGCGGATGTGGTGATCGTGGGCGGTGGCTTCACCGGCCTTTCCACGGCGCTGCATCTGGCCGAAAAAGGCGTGT
GTGCCATCGTTCTTGAAGGGATCGAGATCGGTTATGGCGGTTCGGGCCGCAATGTCGGTCTGGTGAATGCAGGCATGTGG
GTTATGCCGGACGATCTGCCGGGCGTGCTCGGCCAGAAATATGGCGACCGGCTGCTTGATGTTCTGGGCAAAGCACCGAA
GCTCGTCTTCGAGATCATCGAAAAACACAAGATCGCCTGCGAGGTTGAAAAGCAGGGCACGCTGCACTGTGCGGTTGGCC
AAAAGGGTCTGAAAGAACTTGAAGACCGCCATGAGCAATGGGCGCGCCGCGGCGCCAATGTGAAGCTGCTTGATGCCAGG
GAAACCGAAGCCAGGGTTGGCACAAGGGCCTATACAGGTTCGTTGCTCGATCTGCGGGCAGGCACGGTGCAGCCACTTGC
CTATGTGCGCGGCCTTGCCCATGCGGCCATTGCCGCTGGCGCGACCGTTTTTACCGGCAGTCCGGTGATTGGCGCTGAGG
AAAAGAACGGCAAATGGGTGGTCAGGACCGCCAGAGGTTCCGTGACGGCAAACTGGGTGGTTGTGGCGACCAATGCCTAT
ACCAATGCGCCGTGGGCCGAGGTGCGCGCCGAGCTGGTGCATCTGCCTTATTTCAACATGGCAACCAAGCCGCTTTCCGA
CAATCTGAAGAAGAGCATCCTGCCGGAACGCCAGGGTGTGTGGGATACGAAGGAAGTGCTTTCGTCCTTCCGTTTTGACC
AGCAGGGCCGCCTTGTTTTTGGAAGCGTCGGCGCGCTGCGCAATACGGGTGCTGCCGTGCACAAGGCATGGGCAAAGAAA
TCATTGAAGCGTCTCTTCCCGCAGATTGGCGCGGTGGAGTTCGAGGCCGAATGGTATGGCAAGATCGGCATGACCAATGA
CAGCCTGCCGAAATTCCATCGTCTTGCGCGCAATGTGGTCGGCTTCTCCGGCTATAATGGACGCGGCATTGCACCGGGCA
CGGTGTTTGGAAAACTGCTGTCGCAGCTTGTGTCCGGCGAGATTTCGGAAGCCGATCTGCCGCTGCCGGTCAGCGACCTG
AAGGAACAGAATTTCCGCGGCCTGCGCGAAGCCTATTACGAGGCCGGTGCGCAGGTTGCACATGTGGCCGAAATGGTCAT
CTGA

Upstream 100 bases:

>100_bases
AATTGTTGCGCAAATAACGCTGTCCAACCGGCTTTCGCGGCGTGAAATAGAAGCAGTCGGGCAGGGTGCCCGTGTTTCAC
TATTTTAATGGTTGATCTCA

Downstream 100 bases:

>100_bases
GTTTCCGGGCCGCTCATAAATCCACCATATCCCCTTCCGCAGAGGAATATTCTGCGGAAGGGTTTTGTTTGTCGGGCACC
GGATGGGTGCCGGTTTATTT

Product: hypothetical protein

Products: glycine; formaldehyde; H2O2

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLNDPRSHGLWEKTAPAAPKTTALQGDLAADVVIVGGGFTGLSTALHLAEKGVCAIVLEGIEIGYGGSGRNVGLVNAGMW
VMPDDLPGVLGQKYGDRLLDVLGKAPKLVFEIIEKHKIACEVEKQGTLHCAVGQKGLKELEDRHEQWARRGANVKLLDAR
ETEARVGTRAYTGSLLDLRAGTVQPLAYVRGLAHAAIAAGATVFTGSPVIGAEEKNGKWVVRTARGSVTANWVVVATNAY
TNAPWAEVRAELVHLPYFNMATKPLSDNLKKSILPERQGVWDTKEVLSSFRFDQQGRLVFGSVGALRNTGAAVHKAWAKK
SLKRLFPQIGAVEFEAEWYGKIGMTNDSLPKFHRLARNVVGFSGYNGRGIAPGTVFGKLLSQLVSGEISEADLPLPVSDL
KEQNFRGLREAYYEAGAQVAHVAEMVI

Sequences:

>Translated_427_residues
MLNDPRSHGLWEKTAPAAPKTTALQGDLAADVVIVGGGFTGLSTALHLAEKGVCAIVLEGIEIGYGGSGRNVGLVNAGMW
VMPDDLPGVLGQKYGDRLLDVLGKAPKLVFEIIEKHKIACEVEKQGTLHCAVGQKGLKELEDRHEQWARRGANVKLLDAR
ETEARVGTRAYTGSLLDLRAGTVQPLAYVRGLAHAAIAAGATVFTGSPVIGAEEKNGKWVVRTARGSVTANWVVVATNAY
TNAPWAEVRAELVHLPYFNMATKPLSDNLKKSILPERQGVWDTKEVLSSFRFDQQGRLVFGSVGALRNTGAAVHKAWAKK
SLKRLFPQIGAVEFEAEWYGKIGMTNDSLPKFHRLARNVVGFSGYNGRGIAPGTVFGKLLSQLVSGEISEADLPLPVSDL
KEQNFRGLREAYYEAGAQVAHVAEMVI
>Mature_427_residues
MLNDPRSHGLWEKTAPAAPKTTALQGDLAADVVIVGGGFTGLSTALHLAEKGVCAIVLEGIEIGYGGSGRNVGLVNAGMW
VMPDDLPGVLGQKYGDRLLDVLGKAPKLVFEIIEKHKIACEVEKQGTLHCAVGQKGLKELEDRHEQWARRGANVKLLDAR
ETEARVGTRAYTGSLLDLRAGTVQPLAYVRGLAHAAIAAGATVFTGSPVIGAEEKNGKWVVRTARGSVTANWVVVATNAY
TNAPWAEVRAELVHLPYFNMATKPLSDNLKKSILPERQGVWDTKEVLSSFRFDQQGRLVFGSVGALRNTGAAVHKAWAKK
SLKRLFPQIGAVEFEAEWYGKIGMTNDSLPKFHRLARNVVGFSGYNGRGIAPGTVFGKLLSQLVSGEISEADLPLPVSDL
KEQNFRGLREAYYEAGAQVAHVAEMVI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=421, Percent_Identity=33.4916864608076, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 45997; Mature: 45997

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDPRSHGLWEKTAPAAPKTTALQGDLAADVVIVGGGFTGLSTALHLAEKGVCAIVLEG
CCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEEEC
IEIGYGGSGRNVGLVNAGMWVMPDDLPGVLGQKYGDRLLDVLGKAPKLVFEIIEKHKIAC
EEECCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEE
EVEKQGTLHCAVGQKGLKELEDRHEQWARRGANVKLLDARETEARVGTRAYTGSLLDLRA
EECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCCCEEEECC
GTVQPLAYVRGLAHAAIAAGATVFTGSPVIGAEEKNGKWVVRTARGSVTANWVVVATNAY
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCC
TNAPWAEVRAELVHLPYFNMATKPLSDNLKKSILPERQGVWDTKEVLSSFRFDQQGRLVF
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEE
GSVGALRNTGAAVHKAWAKKSLKRLFPQIGAVEFEAEWYGKIGMTNDSLPKFHRLARNVV
ECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHC
GFSGYNGRGIAPGTVFGKLLSQLVSGEISEADLPLPVSDLKEQNFRGLREAYYEAGAQVA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
HVAEMVI
HHHHHCC
>Mature Secondary Structure
MLNDPRSHGLWEKTAPAAPKTTALQGDLAADVVIVGGGFTGLSTALHLAEKGVCAIVLEG
CCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEEEC
IEIGYGGSGRNVGLVNAGMWVMPDDLPGVLGQKYGDRLLDVLGKAPKLVFEIIEKHKIAC
EEECCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEE
EVEKQGTLHCAVGQKGLKELEDRHEQWARRGANVKLLDARETEARVGTRAYTGSLLDLRA
EECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCCCEEEECC
GTVQPLAYVRGLAHAAIAAGATVFTGSPVIGAEEKNGKWVVRTARGSVTANWVVVATNAY
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCC
TNAPWAEVRAELVHLPYFNMATKPLSDNLKKSILPERQGVWDTKEVLSSFRFDQQGRLVF
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEE
GSVGALRNTGAAVHKAWAKKSLKRLFPQIGAVEFEAEWYGKIGMTNDSLPKFHRLARNVV
ECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHC
GFSGYNGRGIAPGTVFGKLLSQLVSGEISEADLPLPVSDLKEQNFRGLREAYYEAGAQVA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
HVAEMVI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]