Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 163844912

GI number: 163844912

Start: 755246

End: 756859

Strand: Reverse

Name: appA [H]

Synonym: BSUIS_B0778

Alternate gene names: 163844912

Gene position: 756859-755246 (Counterclockwise)

Preceding gene: 163844915

Following gene: 163844911

Centisome position: 54.03

GC content: 57.06

Gene sequence:

>1614_bases
ATGTCTGGGTTTGTTGCGTCGCGCAGGGCGTTTCTCGTTGGCTCGACCTCTCTGGCGGCGCTCGCATTCTGGCCAAAAGC
TACATGGGCAGCGCAAGAAGTGCAGAAGGGTGGGCGGCTGGTTGTGGCAGCGGATTCCGAGCCACGCAATCTGAACCCGG
CCATTATCGCTTCCAACGGCGTATTTTTTGTGGCGAGCAAGGTGATCGAGCCTCTGGCGGAGGCTGCCTATGAAGGGCAA
GGCGGTCTTGCGCCGCGTCTTGCCACAAGCTGGGAAGGCTCTGCCGACGGCCTTACAGCCACTTTTCGCTTGCGTGAAGG
CGTCAACTGGCACGATGGCAAGCCGTTTACTTCAGCCGATGTCGCCTTTTCCGCGATGGAAGTCTGGAAGCCACTGCAAA
ACCTTGGCCGTGTCGTGTTCAAATCGCTGGAAAAGGTCGAGACGCCGGACGAACATACCGCAATCTTCCATTTTTCCGAG
CCAACGCCATTTCAGCTCATCCGCAATGCGCTGCCTGCGCTGACCGCCGTTTTGCCAAAGCATGTCTTTGCCGGCACCGA
TATTGCCGCCAATCCGGCAAACGAAAAGCTCGTCGGCACGGGGCCGTTCAAATATGCAGAACACAAGACGGGCGAATATT
ATCGGCTGGTGAAAAATGCGGACTATTGGGGCGAGGGTGAGCCCTATCTGGATGAAGTAATCTACCGTGTGCTGCCGGAT
CGTGCGGCCGCGGCTGATGCGCTGGAAGCCGGGGAGATCGATCTTGCAGCTTTTTCCGCAGTGCCGCTTGCCGATCTCGA
CCGTATATCGAAAGTGCCGGACCTTGCCGTTATTTCCAAAGGCTATGAAGGGCTGACCTATCAGCTTGTGGTGGAAATCA
ATCACCGTCGCAAGGAACTGGCAGACCTGAAGGTACGCCAGGCAATCGCCCATGCCATCGACCGCGATTTCGTGGTGAAG
ACGATTTTCCAGGGGTTTGCCGTGCCCTCGACCGGGCCGGTGCCGCGCTATGACAGCCAGTTCTATACGGATGATGTGCC
GACCTATCCCTTTGATGTGAAAAAGGCGAACGCACTTTTGGATGAAGCGGGCTACAGGCGCGGCAAGGACGGAATGCGCT
TTGCGCTCAAGCTTCTGCCTGCACCCTATTTCAACGAGACGAAGCAGTTCGGTGATTATCTGCGGCAGGCGCTTGCCGCC
ATCGGTATCGATGCGCACATCGTCAACAATGATGCCGCCGCGCATCAGAAGGCAGTCTATACGGATCACGATTTCGATCT
CGCCATCACGCCGACCGTCTATCGCGGCGATCCGGCCATTTCGACCACCATTCTGGTGGAAAGCGGCATTCCGGCGGGTG
TTCCTTTCTCCAATCAGGGCGGCTATGCCAATGCCGAACTCGATGCATTGATTGCCAAAGCCGCAAGCACGACCGATGAA
AAGGAACGCACCGCGCTCTACCAAAAATTCCAGCAGATGGTGGAAGCAGACCTGCCGCTCATCAATGTCGCGGAATGGGG
GTTCATTACGGTTGCCAGCGAGCGCGTGAAAAATGTTTCCAACAATCCGCGCTGGGCGGTTTCCAACTGGGCTGACACAT
GGCTGGAACCATGA

Upstream 100 bases:

>100_bases
GATTTTCGATATTTATTGTCGCGGCAACGGATTTGGCCCTGCCATTTTGAGACGGCTGATATAGAAATAGGCCGTATCGA
AGAAAAATGGGGCGGTAAGC

Downstream 100 bases:

>100_bases
TGAAGCAACCGGATTGATTGTATCTTGCTGACGTGAAACAGATTCTGGCCCTTTTGCGAAAACGTCTGGTTGGCAGCATG
GTGGTGCTGCTGATTGTCGT

Product: hypothetical protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQ
GGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSE
PTPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLVGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPDLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVK
TIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAA
IGIDAHIVNNDAAAHQKAVYTDHDFDLAITPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP

Sequences:

>Translated_537_residues
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQ
GGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSE
PTPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLVGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPDLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVK
TIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAA
IGIDAHIVNNDAAAHQKAVYTDHDFDLAITPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
>Mature_536_residues
SGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQG
GLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEP
TPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLVGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPDR
AAAADALEAGEIDLAAFSAVPLADLDRISKVPDLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVKT
IFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAI
GIDAHIVNNDAAAHQKAVYTDHDFDLAITPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDEK
ERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=26.3671875, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787052, Length=449, Percent_Identity=28.0623608017817, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=498, Percent_Identity=26.3052208835341, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=512, Percent_Identity=25.1953125, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787495, Length=507, Percent_Identity=23.6686390532544, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=24.6435845213849, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081878, Length=518, Percent_Identity=24.3243243243243, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787551, Length=448, Percent_Identity=22.5446428571429, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58750; Mature: 58619

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNG
CCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCC
VFFVASKVIEPLAEAAYEGQGGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
VAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEPTPFQLIRNALPALTAVLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
HVFAGTDIAANPANEKLVGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
HHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPDLAVISKGYEGLTYQLVVEINHRRKEL
HHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEEEECHHHHHH
ADLKVRQAIAHAIDRDFVVKTIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAIGIDAHIVNNDAAAHQKAVY
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCEE
TDHDFDLAITPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
CCCCCCEEECCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHCCCCCCCEEECCCHHHCCCC
>Mature Secondary Structure 
SGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNG
CCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCC
VFFVASKVIEPLAEAAYEGQGGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
VAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEPTPFQLIRNALPALTAVLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
HVFAGTDIAANPANEKLVGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
HHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPDLAVISKGYEGLTYQLVVEINHRRKEL
HHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEEEECHHHHHH
ADLKVRQAIAHAIDRDFVVKTIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAIGIDAHIVNNDAAAHQKAVY
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCEE
TDHDFDLAITPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
CCCCCCEEECCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHCCCCCCCEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]