Click here to switch to the map view.

The map label for this gene is thcD [H]

Identifier: 163844860

GI number: 163844860

Start: 702039

End: 703268

Strand: Reverse

Name: thcD [H]

Synonym: BSUIS_B0721

Alternate gene names: 163844860

Gene position: 703268-702039 (Counterclockwise)

Preceding gene: 163844861

Following gene: 163844858

Centisome position: 50.2

GC content: 60.0

Gene sequence:

>1230_bases
ATGGACGACAGATGCATTATCATCGGGGCTGGCCATGCCGGTTCGCAGGCTGCGGTCAGCCTTCGCCAGGAAGGGTATGC
AGGCGAAATCATTCTCATCAATGACGAAACGGATATTCCCTACCACAAGCCGCCGCTCTCCAAGTCCTATCTGAAAGCGC
CGGAAAAAGGCAGTCTGGTGCTGCGCCCGGAATCGGCCTATCGCGACAATAATATTGAGATGCTCTTTGGCGCGCATGTC
GATGCCGTATCCATTGCGGAGCGGACGGTGACGCTTGGCGACGGCCGCGTGTTGCCATGGTCTGAACTGGTTTTCGCAAC
GGGTGCGAGGGCGCGCATCCCCGATGTGCCGGGGGTTGCGCTTGAGGGCGTTGTAACACTGCGGCGGATGGAAGATGCGC
GCCGGATCGCCGCCATGATGCCGGACGTGCGCAATGTCGTGATTATCGGCGGCGGCTTCATCGGCCTTGAAATGGCGCAT
AGCGCGATTGCGCTCGGCAAGAAGACCGTATTGATCGAGGCTGCCCCTCGCGTCCTTGGCCGTTCGGTTGCGGCGCCCGT
TTCCGCCCATGTGGAGGCGCGAAGCCGCGCTGCCGATATCACACTCCTGACGGGCCTTGGCGTCGCTTCCATCGAAGGGG
AGAATGGGCGCGCGATAGGTGTAACGGCTGGCGATGGCACATTTTTCCCGGCTGATCTCGTGGTTATCGGCACGGGTGCG
GTTCCCAATGTGGAACTGGCGGCGAAAGCGGGCCTCAGCATCGATAACGGGATCAGGGTGGACGAGCATATGCGGACGTC
TGCCCCCCATGTCTATGCGATAGGCGATTGTGCGAGCTATGCTCATTTTCATGCAGGCCGCCATGTGCGCCTGGAATCGG
TGCAGAATGCCACCGATCAGGCAAAGCATGTCGCACGCACGATCGTTGGGCGGGAGACGCCTTATCGCGAGGTTGCCTGG
TTCTGGTCCGATCAGGGCGATATGAAGCTTCAGACCGCCGGTCTTTCGTTTGATGCTGACCGGCATGTTCTTTCCGGGGA
GCCGGAAGAGAATGCTTTCTCGGTCTTTCATTTCAAGGGCGACAGGCTGGTGGCGGTCGATTCCATCAATCGCCCGGCAG
ATCATATGATTGCGCGTCGTCTGCTTGCGGCGGGGATCAGCCCAAGCGAGGCCGATATTATGGCTGGTATGCCGCGCCTC
AAGGAATTGCTGGCGGTTGTGGCGCGATAA

Upstream 100 bases:

>100_bases
GCTTTGCCGAACCGCTCCATCTCCCTGTTGTGTCCAGACATGCTTTATTCACGCGATGGGCCGCGACGCATTTATGATGA
GCGGAAATTTCGGAGGTCCT

Downstream 100 bases:

>100_bases
AAGTTTTATGGAAAAGACCGGACTTGGGGGGGAGGAGAGTGTCCGGTCTTTGATCGCCGAGGGAATGGGAGGAGGAGAAA
CACCCGGCGATATTCTGGAA

Product: hypothetical protein

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDDRCIIIGAGHAGSQAAVSLRQEGYAGEIILINDETDIPYHKPPLSKSYLKAPEKGSLVLRPESAYRDNNIEMLFGAHV
DAVSIAERTVTLGDGRVLPWSELVFATGARARIPDVPGVALEGVVTLRRMEDARRIAAMMPDVRNVVIIGGGFIGLEMAH
SAIALGKKTVLIEAAPRVLGRSVAAPVSAHVEARSRAADITLLTGLGVASIEGENGRAIGVTAGDGTFFPADLVVIGTGA
VPNVELAAKAGLSIDNGIRVDEHMRTSAPHVYAIGDCASYAHFHAGRHVRLESVQNATDQAKHVARTIVGRETPYREVAW
FWSDQGDMKLQTAGLSFDADRHVLSGEPEENAFSVFHFKGDRLVAVDSINRPADHMIARRLLAAGISPSEADIMAGMPRL
KELLAVVAR

Sequences:

>Translated_409_residues
MDDRCIIIGAGHAGSQAAVSLRQEGYAGEIILINDETDIPYHKPPLSKSYLKAPEKGSLVLRPESAYRDNNIEMLFGAHV
DAVSIAERTVTLGDGRVLPWSELVFATGARARIPDVPGVALEGVVTLRRMEDARRIAAMMPDVRNVVIIGGGFIGLEMAH
SAIALGKKTVLIEAAPRVLGRSVAAPVSAHVEARSRAADITLLTGLGVASIEGENGRAIGVTAGDGTFFPADLVVIGTGA
VPNVELAAKAGLSIDNGIRVDEHMRTSAPHVYAIGDCASYAHFHAGRHVRLESVQNATDQAKHVARTIVGRETPYREVAW
FWSDQGDMKLQTAGLSFDADRHVLSGEPEENAFSVFHFKGDRLVAVDSINRPADHMIARRLLAAGISPSEADIMAGMPRL
KELLAVVAR
>Mature_409_residues
MDDRCIIIGAGHAGSQAAVSLRQEGYAGEIILINDETDIPYHKPPLSKSYLKAPEKGSLVLRPESAYRDNNIEMLFGAHV
DAVSIAERTVTLGDGRVLPWSELVFATGARARIPDVPGVALEGVVTLRRMEDARRIAAMMPDVRNVVIIGGGFIGLEMAH
SAIALGKKTVLIEAAPRVLGRSVAAPVSAHVEARSRAADITLLTGLGVASIEGENGRAIGVTAGDGTFFPADLVVIGTGA
VPNVELAAKAGLSIDNGIRVDEHMRTSAPHVYAIGDCASYAHFHAGRHVRLESVQNATDQAKHVARTIVGRETPYREVAW
FWSDQGDMKLQTAGLSFDADRHVLSGEPEENAFSVFHFKGDRLVAVDSINRPADHMIARRLLAAGISPSEADIMAGMPRL
KELLAVVAR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=400, Percent_Identity=30.75, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI21389617, Length=372, Percent_Identity=31.7204301075269, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI65787454, Length=400, Percent_Identity=30.75, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI22202629, Length=352, Percent_Identity=25, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4757732, Length=352, Percent_Identity=25, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788892, Length=380, Percent_Identity=33.6842105263158, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=28.391167192429, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789765, Length=283, Percent_Identity=27.9151943462898, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=377, Percent_Identity=28.1167108753316, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639257, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281359715, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281359713, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639250, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI18543267, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639252, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24585130, Length=383, Percent_Identity=25.5874673629243, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=24.4444444444444, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=25, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43659; Mature: 43659

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRCIIIGAGHAGSQAAVSLRQEGYAGEIILINDETDIPYHKPPLSKSYLKAPEKGSLV
CCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCEE
LRPESAYRDNNIEMLFGAHVDAVSIAERTVTLGDGRVLPWSELVFATGARARIPDVPGVA
ECCCHHCCCCCEEEEEECCCCEEECCEEEEEECCCCCCCHHHHHEECCCCCCCCCCCCHH
LEGVVTLRRMEDARRIAAMMPDVRNVVIIGGGFIGLEMAHSAIALGKKTVLIEAAPRVLG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEEECHHHHH
RSVAAPVSAHVEARSRAADITLLTGLGVASIEGENGRAIGVTAGDGTFFPADLVVIGTGA
HHHCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEECCCCCCCCCEEEEEECCC
VPNVELAAKAGLSIDNGIRVDEHMRTSAPHVYAIGDCASYAHFHAGRHVRLESVQNATDQ
CCCEEEHHHCCCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCCCEEEEHHHHCHHHH
AKHVARTIVGRETPYREVAWFWSDQGDMKLQTAGLSFDADRHVLSGEPEENAFSVFHFKG
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
DRLVAVDSINRPADHMIARRLLAAGISPSEADIMAGMPRLKELLAVVAR
CEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MDDRCIIIGAGHAGSQAAVSLRQEGYAGEIILINDETDIPYHKPPLSKSYLKAPEKGSLV
CCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCEE
LRPESAYRDNNIEMLFGAHVDAVSIAERTVTLGDGRVLPWSELVFATGARARIPDVPGVA
ECCCHHCCCCCEEEEEECCCCEEECCEEEEEECCCCCCCHHHHHEECCCCCCCCCCCCHH
LEGVVTLRRMEDARRIAAMMPDVRNVVIIGGGFIGLEMAHSAIALGKKTVLIEAAPRVLG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEEECHHHHH
RSVAAPVSAHVEARSRAADITLLTGLGVASIEGENGRAIGVTAGDGTFFPADLVVIGTGA
HHHCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEECCCCCCCCCEEEEEECCC
VPNVELAAKAGLSIDNGIRVDEHMRTSAPHVYAIGDCASYAHFHAGRHVRLESVQNATDQ
CCCEEEHHHCCCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCCCEEEEHHHHCHHHH
AKHVARTIVGRETPYREVAWFWSDQGDMKLQTAGLSFDADRHVLSGEPEENAFSVFHFKG
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
DRLVAVDSINRPADHMIARRLLAAGISPSEADIMAGMPRLKELLAVVAR
CEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]