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The map label for this gene is cueO [H]

Identifier: 163844833

GI number: 163844833

Start: 670450

End: 672054

Strand: Reverse

Name: cueO [H]

Synonym: BSUIS_B0689

Alternate gene names: 163844833

Gene position: 672054-670450 (Counterclockwise)

Preceding gene: 163844834

Following gene: 163844832

Centisome position: 47.97

GC content: 58.75

Gene sequence:

>1605_bases
ATGACTGGAATTACCCGCCGGCGCCTGCTTGCGCTTGGCGCAAGTGCTGCCTGTGTTGCTGCCCTTCGCCCGCTTGGGGC
CTTTGCGCAGGATGCGCATCAGCATATGAGGCACGGCGCCGTGGATGCGCCTGTATTAAAGGCGCCACCTCTGCCTCTGC
CGCCGCTTGTCGAGCCGGATGCTTCTGGCATCGTGAGGTTGAAAGTGCAAAAGGGCCGCCACTCCTTCGCGAAGGGAAGC
ACGGCGGCCTCTGCCGGTATTAACGGTGCCTATCTTGGGCCTCTGGTGCGCCTGATGAGCGGTGAGAGCGTTACGCTTTC
CGTTGAAAACGCGATGGATGAGGAAACGACGCTGCATTGGCACGGCCTGTTCGTTCCCTCACATCTCGATGGCGGGCCGC
ATAATGTTATTGCACCCGGTGCAAAATGGGAGCCGAAGGTGGCTGTCAACCAGCCGGCCTCGTTCAACTGGTTCCATCCG
CATCTGCATGGCCACACTGCCCGTCAGGCACATATGAGCATTGCCGGGCTGATGATCGTGCGTGACGGCAAGGACGCGGA
GCGCGGCCTGCCGGAAACTTACGGCGTGGACGATCTTCCGCTGCTCCTGCAAGACCGGCGCGTCATTGAGGGCGATGCGG
TCTATGCGCCGGATATCATGGACCTGATACATGGGTTCCGTGGTGACTGGCTGATCGTCAACGGGGCTATTGCACCGGAA
GCACGGGTTCCGGCAGCGATGGTGCGCCTGCGCTTGCTCAACGGTGCCAATGCGCGCAATTTCCACATTCGCTTTGCCGA
TGGCCGTCCCTTGCTGGTCATTGCTTCCGATGGTGGCTTCATAAGCCAGCCGGTATCGATTGAACAACTGACGATCAGCC
CCGGCGAACGGTATGAAGTGCTGGTGGATTTTTCAAACGGCGAGGCGGTCGATCTTGTCACTTATGGGGATAATGGCAGC
GGCGATGGCTTGCATCTCATGCGCTTTGCCGTTGATCCGGCGCTGGAGGGCAGGGTGGCGAAGCCGCCGGTGAGCCTCGA
TGGTCCGGCTGCGCCAGACGAAAAGCTTTCCGTTCAAAGGCGCTCCTTTTTCTTCGATGAACGCATGGCTGAAAATATGA
AGCTAATGATGCGACAGCCTTCCAGCAATCCCCATGCCAGCGGGGACGACATGGATCATATGGAGATGGGTTCGATGGCT
GGCATGGACCATGATATGCATGGCAGCCGAAGTGCAGCCGACGCAGGCCCGGCACTCGATGCGCTGACCTCGGGCGTGCA
GATGGCAATTGCCGACAAGCCCTTCGACATGGAACGCATAGATGTGGAAGCAAAGCTCGGTTCCTGGGAAATATGGGAAC
TGACTAGCCGAGAAATGGCGCATCCATTCCATATCCACGGCGCATCGTTTCGTATTCTTTCCATGAACGGCAAAAAGCCG
CCTGCCCATCAGACGGGCTGGAAGGACACGGCACTGATCGACGGCAAGGCTGAAATCCTCGTCCATTTCGACCGTGAAGC
GGCCCGCAGCCACCCGTTCATGTTCCATTGTCATTTGCTGGAACATGAGGACGTGGGCATGATGGCGCAATTCGTGACCG
TTTGA

Upstream 100 bases:

>100_bases
CGCATGGAGTGTTGCGCCCTCCAATAGTTCTTCATGCCTGATAGAACAAATCGCTGCCGGTTTTCCGGCCGCAATGACGA
TTATTGTCTGGAGACTTATC

Downstream 100 bases:

>100_bases
GAGCAAGGCCCGATGCGCCCGGCTCATCGGGCCTTGATTGACCTTATGCGGTGGTAAGCTCCTTCAAAGAGCGTGGTGCA
TTGCCATCCCGGTGTTCTTA

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTGITRRRLLALGASAACVAALRPLGAFAQDAHQHMRHGAVDAPVLKAPPLPLPPLVEPDASGIVRLKVQKGRHSFAKGS
TAASAGINGAYLGPLVRLMSGESVTLSVENAMDEETTLHWHGLFVPSHLDGGPHNVIAPGAKWEPKVAVNQPASFNWFHP
HLHGHTARQAHMSIAGLMIVRDGKDAERGLPETYGVDDLPLLLQDRRVIEGDAVYAPDIMDLIHGFRGDWLIVNGAIAPE
ARVPAAMVRLRLLNGANARNFHIRFADGRPLLVIASDGGFISQPVSIEQLTISPGERYEVLVDFSNGEAVDLVTYGDNGS
GDGLHLMRFAVDPALEGRVAKPPVSLDGPAAPDEKLSVQRRSFFFDERMAENMKLMMRQPSSNPHASGDDMDHMEMGSMA
GMDHDMHGSRSAADAGPALDALTSGVQMAIADKPFDMERIDVEAKLGSWEIWELTSREMAHPFHIHGASFRILSMNGKKP
PAHQTGWKDTALIDGKAEILVHFDREAARSHPFMFHCHLLEHEDVGMMAQFVTV

Sequences:

>Translated_534_residues
MTGITRRRLLALGASAACVAALRPLGAFAQDAHQHMRHGAVDAPVLKAPPLPLPPLVEPDASGIVRLKVQKGRHSFAKGS
TAASAGINGAYLGPLVRLMSGESVTLSVENAMDEETTLHWHGLFVPSHLDGGPHNVIAPGAKWEPKVAVNQPASFNWFHP
HLHGHTARQAHMSIAGLMIVRDGKDAERGLPETYGVDDLPLLLQDRRVIEGDAVYAPDIMDLIHGFRGDWLIVNGAIAPE
ARVPAAMVRLRLLNGANARNFHIRFADGRPLLVIASDGGFISQPVSIEQLTISPGERYEVLVDFSNGEAVDLVTYGDNGS
GDGLHLMRFAVDPALEGRVAKPPVSLDGPAAPDEKLSVQRRSFFFDERMAENMKLMMRQPSSNPHASGDDMDHMEMGSMA
GMDHDMHGSRSAADAGPALDALTSGVQMAIADKPFDMERIDVEAKLGSWEIWELTSREMAHPFHIHGASFRILSMNGKKP
PAHQTGWKDTALIDGKAEILVHFDREAARSHPFMFHCHLLEHEDVGMMAQFVTV
>Mature_533_residues
TGITRRRLLALGASAACVAALRPLGAFAQDAHQHMRHGAVDAPVLKAPPLPLPPLVEPDASGIVRLKVQKGRHSFAKGST
AASAGINGAYLGPLVRLMSGESVTLSVENAMDEETTLHWHGLFVPSHLDGGPHNVIAPGAKWEPKVAVNQPASFNWFHPH
LHGHTARQAHMSIAGLMIVRDGKDAERGLPETYGVDDLPLLLQDRRVIEGDAVYAPDIMDLIHGFRGDWLIVNGAIAPEA
RVPAAMVRLRLLNGANARNFHIRFADGRPLLVIASDGGFISQPVSIEQLTISPGERYEVLVDFSNGEAVDLVTYGDNGSG
DGLHLMRFAVDPALEGRVAKPPVSLDGPAAPDEKLSVQRRSFFFDERMAENMKLMMRQPSSNPHASGDDMDHMEMGSMAG
MDHDMHGSRSAADAGPALDALTSGVQMAIADKPFDMERIDVEAKLGSWEIWELTSREMAHPFHIHGASFRILSMNGKKPP
AHQTGWKDTALIDGKAEILVHFDREAARSHPFMFHCHLLEHEDVGMMAQFVTV

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=497, Percent_Identity=40.2414486921529, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1789394, Length=318, Percent_Identity=38.0503144654088, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 58012; Mature: 57881

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGITRRRLLALGASAACVAALRPLGAFAQDAHQHMRHGAVDAPVLKAPPLPLPPLVEPD
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
ASGIVRLKVQKGRHSFAKGSTAASAGINGAYLGPLVRLMSGESVTLSVENAMDEETTLHW
CCCEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEE
HGLFVPSHLDGGPHNVIAPGAKWEPKVAVNQPASFNWFHPHLHGHTARQAHMSIAGLMIV
EEEEECCCCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHEEEEEEEE
RDGKDAERGLPETYGVDDLPLLLQDRRVIEGDAVYAPDIMDLIHGFRGDWLIVNGAIAPE
ECCCHHHHCCCCCCCCCCCCHHCCCCEEECCCCEECCHHHHHHHCCCCCEEEEECCCCCC
ARVPAAMVRLRLLNGANARNFHIRFADGRPLLVIASDGGFISQPVSIEQLTISPGERYEV
CCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEE
LVDFSNGEAVDLVTYGDNGSGDGLHLMRFAVDPALEGRVAKPPVSLDGPAAPDEKLSVQR
EEEECCCCEEEEEEECCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RSFFFDERMAENMKLMMRQPSSNPHASGDDMDHMEMGSMAGMDHDMHGSRSAADAGPALD
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALTSGVQMAIADKPFDMERIDVEAKLGSWEIWELTSREMAHPFHIHGASFRILSMNGKKP
HHHCCEEEEEECCCCCCEEEEEEEECCCEEEEEECHHHCCCEEEEECCEEEEEEECCCCC
PAHQTGWKDTALIDGKAEILVHFDREAARSHPFMFHCHLLEHEDVGMMAQFVTV
CCCCCCCCCEEEECCCEEEEEEECHHHHHCCCEEEEEEEECCCCCCHHHHHHCC
>Mature Secondary Structure 
TGITRRRLLALGASAACVAALRPLGAFAQDAHQHMRHGAVDAPVLKAPPLPLPPLVEPD
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
ASGIVRLKVQKGRHSFAKGSTAASAGINGAYLGPLVRLMSGESVTLSVENAMDEETTLHW
CCCEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEE
HGLFVPSHLDGGPHNVIAPGAKWEPKVAVNQPASFNWFHPHLHGHTARQAHMSIAGLMIV
EEEEECCCCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHEEEEEEEE
RDGKDAERGLPETYGVDDLPLLLQDRRVIEGDAVYAPDIMDLIHGFRGDWLIVNGAIAPE
ECCCHHHHCCCCCCCCCCCCHHCCCCEEECCCCEECCHHHHHHHCCCCCEEEEECCCCCC
ARVPAAMVRLRLLNGANARNFHIRFADGRPLLVIASDGGFISQPVSIEQLTISPGERYEV
CCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEE
LVDFSNGEAVDLVTYGDNGSGDGLHLMRFAVDPALEGRVAKPPVSLDGPAAPDEKLSVQR
EEEECCCCEEEEEEECCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RSFFFDERMAENMKLMMRQPSSNPHASGDDMDHMEMGSMAGMDHDMHGSRSAADAGPALD
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALTSGVQMAIADKPFDMERIDVEAKLGSWEIWELTSREMAHPFHIHGASFRILSMNGKKP
HHHCCEEEEEECCCCCCEEEEEEEECCCEEEEEECHHHCCCEEEEECCEEEEEEECCCCC
PAHQTGWKDTALIDGKAEILVHFDREAARSHPFMFHCHLLEHEDVGMMAQFVTV
CCCCCCCCCEEEECCCEEEEEEECHHHHHCCCEEEEEEEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]