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The map label for this gene is gatA [H]

Identifier: 163844746

GI number: 163844746

Start: 580321

End: 581802

Strand: Reverse

Name: gatA [H]

Synonym: BSUIS_B0591

Alternate gene names: 163844746

Gene position: 581802-580321 (Counterclockwise)

Preceding gene: 163844747

Following gene: 163844745

Centisome position: 41.53

GC content: 60.26

Gene sequence:

>1482_bases
ATGAGTGAACTGACCGCACTGACCATTGCCGAAGCGCGCGACAAGTTGAAAGCCAAGGCCATTACCGCGACCGAGCTGAC
GGACGCCTATCTTTCCGCTATCGATGCCGCCAATGACGCCATCAATGCCTATGTGGCCGTCACCCACGATCAGGCGCGTT
CCATGGCCAAGGCTTCGGATGAACGCATCGCGAAGGGCGAAGCGGGCGCATTGGAGGGCATTCCGCTCGGCGTGAAGGAC
CTGTTTGCAACCAAGGGCGTGCACACGCAGGCCTGCTCGCACATTCTGGACGGTTTCAAGCCGGAATATGAATCGACCGT
CACCGCCAATTTATGGGCTGATGGCGCGGTCATGCTCGGCAAGCTCAATATGGATGAGTTCGCCATGGGCTCCTCCAACG
AAACTTCCTATTACGGTCCGGTGAAAAATCCGTGGCGCGCCAAGGGTTCCAATGCCGATCTGGTGCCGGGCGGCTCGTCC
GGCGGTTCGGCTGCCGCCGTTGCCGCGCATCTTTGCGCGGGCGCGACTGCGACCGATACGGGCGGCTCGATCCGCCAGCC
TGCCGCCTTTACCGGTACGGTCGGCATCAAGCCAACCTATGGCCGTGTTTCGCGCTGGGGTACGGTGGCTTTCGCCTCGT
CGCTCGATCAGGCAGGCCCGATTGCGCGCGACGTGCGCGATGCTGCGATCCTTATGAAGTCCACGGCTTCGCTCGATCTC
AAGGACACAACTTCCGTTGACCTGCCGGTGCCGGATTATGAAGCAGCCCTCGGCAGGTCTGTGAAGGGCATGAAGATCGG
CATTCCAAGGGAATATCGTGTCGATGGCATGCCGGGCGAAATCGAGGAACTCTGGCAGAAGGGCATCCAGTATCTGAAGG
ATGCAGGTGCCGAGATTGTCGATATTTCGCTGCCGCATACCAAATATGCGCTGCCTGCTTATTATATCGTGGCGCCTGCG
GAAGCTTCGTCCAACCTCGCACGTTATGATGGCGTTCGTTATGGCCTGCGCGTGCCCGGCAAGGATATTGCCGACATGTA
CGAACAGACCCGCGCCGCAGGCTTCGGCAAGGAAGTGAAGCGCCGCATCATGATCGGCACCTATGTCCTGTCGGCGGGCT
ATTACGACGCTTATTACCTCCGCGCCCAGAAGGTGCGTACGCTGATCAAGAAGGATTTCGAGGACGTTTTCGCCAAGGGT
GTGGATGCTATCCTGACCCCGGCCACGCCTTCGGCGGCTTTCGGCCTTGCCGATGAAGTCCTCGCCAACGACCCGGTCAA
GATGTACCTGAACGATATCTTTACCGTCACCGTGAACATGGCGGGATTGCCGGGCATCGCGGTGCCTGCCGGGCTGAACG
GGCAGGGCCTGCCGCTTGGCCTCCAGCTTATCGGCCGCCCCTTCGAGGAAGAAACGCTGTTTCAGGCAGCCCATGTCATC
GAGCAGGCTGCCGGGCGTTTCACGCCAGCGAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
ACGGAAGACAATTTCTTCGTGGTTCCGAAGGTCGTGGAGTAAGGCCTTCCAGCAACACACGAAAGCGCCCGCCGGAGCGG
CGCATTGATAGGATTTGACG

Downstream 100 bases:

>100_bases
AACCGGGGTTCTATCGGCCCAGGATTGCGTCGGCCTGAAAATCGGCCAATTCACGATGCAATGCCAAAAGATATCGAGCG
CCCCGCAATATCGGGGCGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKD
LFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSTASLDL
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKG
VDAILTPATPSAAFGLADEVLANDPVKMYLNDIFTVTVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
EQAAGRFTPAKWW

Sequences:

>Translated_493_residues
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKD
LFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSTASLDL
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKG
VDAILTPATPSAAFGLADEVLANDPVKMYLNDIFTVTVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
EQAAGRFTPAKWW
>Mature_492_residues
SELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASDERIAKGEAGALEGIPLGVKDL
FATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSG
GSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSTASLDLK
DTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGV
DAILTPATPSAAFGLADEVLANDPVKMYLNDIFTVTVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVIE
QAAGRFTPAKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=41.010101010101, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI195972892, Length=509, Percent_Identity=25.9332023575639, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI166795287, Length=258, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=36.036036036036, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=534, Percent_Identity=26.5917602996255, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538252, Length=269, Percent_Identity=24.5353159851301, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=534, Percent_Identity=23.2209737827715, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=205, Percent_Identity=33.6585365853659, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=208, Percent_Identity=31.7307692307692, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=35.1851851851852, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=429, Percent_Identity=26.8065268065268, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=38.0368098159509, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=23.6, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=23.6, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=23.6, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=23.6, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=23.9299610894942, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI161078093, Length=528, Percent_Identity=25.1893939393939, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=514, Percent_Identity=25.875486381323, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=519, Percent_Identity=23.6994219653179, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648437, Length=519, Percent_Identity=23.6994219653179, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550774, Length=520, Percent_Identity=23.4615384615385, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648441, Length=447, Percent_Identity=23.489932885906, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=447, Percent_Identity=23.489932885906, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52417; Mature: 52286

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
ERIAKGEAGALEGIPLGVKDLFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSTASLDL
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIV
CCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGVDAILTPATPSAAFGLADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHCCHHHH
LANDPVKMYLNDIFTVTVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
HCCCCHHHHHHCEEEEEEEECCCCCEECCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHH
EQAAGRFTPAKWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
SELTALTIAEARDKLKAKAITATELTDAYLSAIDAANDAINAYVAVTHDQARSMAKASD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
ERIAKGEAGALEGIPLGVKDLFATKGVHTQACSHILDGFKPEYESTVTANLWADGAVMLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKGSNADLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRVSRWGTVAFASSLDQAGPIARDVRDAAILMKSTASLDL
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
KDTTSVDLPVPDYEAALGRSVKGMKIGIPREYRVDGMPGEIEELWQKGIQYLKDAGAEIV
CCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIADMYEQTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDVFAKGVDAILTPATPSAAFGLADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHCCHHHH
LANDPVKMYLNDIFTVTVNMAGLPGIAVPAGLNGQGLPLGLQLIGRPFEEETLFQAAHVI
HCCCCHHHHHHCEEEEEEEECCCCCEECCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHH
EQAAGRFTPAKWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA