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The map label for this gene is alkB [H]

Identifier: 163844692

GI number: 163844692

Start: 514116

End: 514754

Strand: Reverse

Name: alkB [H]

Synonym: BSUIS_B0529

Alternate gene names: 163844692

Gene position: 514754-514116 (Counterclockwise)

Preceding gene: 163844693

Following gene: 163844690

Centisome position: 36.75

GC content: 57.59

Gene sequence:

>639_bases
ATGAAAGACCTCTTTGACAATTTGAACACGCGCGAGATTATGGCGCCGGGGGCCGTGCTTCTGCGCGGTTTTGCGCTTGC
CTGTGAAGAATCGATCCTGAGCGCGGTGGCCAGGGTTTGCGCAGCAGCACCTTTTCGGCATATGACCACACCCGGCGGTT
ATCGCATGTCTGTCGCCATGACCAATTGCGGCCCTGTGGGATGGGTGACAGACCGCACCGGATACCGATACAGCCCTGCC
GATCCCGAAACCGGCAAGCCATGGCCATCCATGCCCGATACGTTCCTTGCCCTTGCCCAGGACGCAGCGCGGCAAGCAGG
CTATCCTGATTTTACGCCTGATGCCTGCCTCATCAATCGTTATGAGCCGGGCGCAAGGCTTTCGCTCCATCAGGACAAGG
ACGAACGGAATTTTTCCAATCCCATCGTTTCCGTTTCACTTGGCCTGCCGGCAACGTTTCAGTTTGGCGGAATGAAACGG
ACGGACCCGATCACCAAATATATCCTGCACCATGGCGACGTGGTTGTCTGGGGCGGGCCGTCGCGGCTTTTCTATCACGG
TATTCTGCCATTGAAGTCTGGCGAGCATGAGCGGCTGGGGCCGTTTCGGCTTAATCTGACCTTCCGAAAGGCGTTCTAG

Upstream 100 bases:

>100_bases
TTTCGGTACGAATGGATGAATAAGCGGGGCTTCGGCCCCGCCTGGGATTTGAAACAGTTTTCGGGACGTTTAAAGATCGG
CCTTATGATAAAAGGGGCTG

Downstream 100 bases:

>100_bases
TGGATTGAATTTGACGTTTGAATCCCGGCTGTTACATATGCTGTTTCAAACGTTAAATTCTAAAAATCCACTAGATACAT
ATACTTACGAGTGGTCTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKDLFDNLNTREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSVAMTNCGPVGWVTDRTGYRYSPA
DPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLINRYEPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKR
TDPITKYILHHGDVVVWGGPSRLFYHGILPLKSGEHERLGPFRLNLTFRKAF

Sequences:

>Translated_212_residues
MKDLFDNLNTREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSVAMTNCGPVGWVTDRTGYRYSPA
DPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLINRYEPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKR
TDPITKYILHHGDVVVWGGPSRLFYHGILPLKSGEHERLGPFRLNLTFRKAF
>Mature_212_residues
MKDLFDNLNTREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSVAMTNCGPVGWVTDRTGYRYSPA
DPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLINRYEPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKR
TDPITKYILHHGDVVVWGGPSRLFYHGILPLKSGEHERLGPFRLNLTFRKAF

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=132, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788541, Length=212, Percent_Identity=63.2075471698113, Blast_Score=272, Evalue=1e-74,
Organism=Drosophila melanogaster, GI45555401, Length=108, Percent_Identity=37.962962962963, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23432; Mature: 23432

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLFDNLNTREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSVAM
CCCHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
TNCGPVGWVTDRTGYRYSPADPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLINR
CCCCCCCEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEC
YEPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKRTDPITKYILHHGDVVVWGGP
CCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHCCCEEEECCC
SRLFYHGILPLKSGEHERLGPFRLNLTFRKAF
CEEEEEECEECCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MKDLFDNLNTREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSVAM
CCCHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
TNCGPVGWVTDRTGYRYSPADPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLINR
CCCCCCCEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEC
YEPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKRTDPITKYILHHGDVVVWGGP
CCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHCCCEEEECCC
SRLFYHGILPLKSGEHERLGPFRLNLTFRKAF
CEEEEEECEECCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]