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The map label for this gene is thlA [H]

Identifier: 163844614

GI number: 163844614

Start: 437143

End: 438333

Strand: Reverse

Name: thlA [H]

Synonym: BSUIS_B0449

Alternate gene names: 163844614

Gene position: 438333-437143 (Counterclockwise)

Preceding gene: 163844615

Following gene: 163844613

Centisome position: 31.29

GC content: 62.3

Gene sequence:

>1191_bases
ATGTCCAAGAGCGATCCTATTGTCATCGTCGGTGCTTCGCGCACCCCCATGGGCGGTTTTCAGGGTGACTTCACAAATGC
GCAGGCAACCGACCTTGGCGCTTCCGCCATCGGCGGCGCGCTTGCCGGTGCAGGCCTTGCCCCGGAAGCCGTCGAGGAGG
TTATCATGGGCTGTGTCCTGCCTGCTGGTCAGGGTCAGGCGCCTGCGCGTCAGGCCTCGCTCAAGGCCGGGCTGCCGCTT
GGCACCGGTGCCACGACCGTCAACAAGATGTGCGGCTCGGGCATGAAGGCCGCGATGCTTGCCCATGACCTCATCCTTGC
CGGTTCCGCCGATGTCATCGTCGCGGGCGGCATGGAAAGCATGACAAATGCCCCCTATCTTCTGCCCAAGGCGCGCGGGG
GCTATCGCATGGGCCATGGACAGGTGCTCGACCATATGTTCCTCGATGGGCTGGAAGATGCCTATGACAAGGGGCGCCTG
ATGGGCACGTTTGCGGAAGATTGCGCCGAGGCCTACCAGTTCACGCGCGAGGCGCAGGACGCATTTGCGATATCCTCCCT
GACCCGCGCGCAGAACGCGATCAAGGATGGTCTTTTTGCGGCGGAAATCACGCCGGTCAAGGTCAAGTCCGGCCGCGCCG
AGGTGGAAGTTACCATTGACGAGCAGCCGGGCAAGGCAAAGCTGGACAAGATCCCCACGCTGCGCCCCGCCTTCCGCGAA
GGCGGCACGGTAACGGCGGCCAATTCCTCTTCCATTTCCGATGGCGCGGCAGCACTTCTGCTCATGCGCGCATCGGAGGC
AGAAAAACGTGGCCTCACCCCGCACGCGGTCATCACCGGCCACGCAACCTATGCCGACAAGCCAAATCTCTTCTCCACGG
CCCCCATTGGCGCGATCCGCAAACTGTCTGAAAAGACTGGCTGGGATTTGAAGGATGTCGATCTCTTCGAGATCAATGAA
GCCTTTGCCGTTGTTGCCATGGCCGCCATGCGCGATCTCGACCTGCCGCACGACAAGGTCAATATTCATGGCGGCGCCTG
TGCACTTGGCCACCCCATCGGCGCATCGGGCGCACGTATTCTCGTAACCCTGCTGGCGGCACTTGAAACACATGGCCTGA
AGCGCGGCATCGCGGGCATCTGCCTTGGCGGCGGCGAGGCAACCGCAATGGGTATCGAAAGGATCATCTGA

Upstream 100 bases:

>100_bases
TCGACGGCGCACTGCGTATGGCCGCCAAGTAAAGGCCGCGAAGTAAAGCCAAGGCAATTTCTGACGAAATTGCGCACAGA
CAAAGCAGCGAGGAACAGTC

Downstream 100 bases:

>100_bases
TGCTTCTGACCGATACGCAGGAACAGATCCGCGAAGCCGCCCGCGACTTCGCACAGGAGCGCCTTGCCCCCGGCGCCGCG
GCGCGTGACCGTGAACATGC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKSDPIVIVGASRTPMGGFQGDFTNAQATDLGASAIGGALAGAGLAPEAVEEVIMGCVLPAGQGQAPARQASLKAGLPL
GTGATTVNKMCGSGMKAAMLAHDLILAGSADVIVAGGMESMTNAPYLLPKARGGYRMGHGQVLDHMFLDGLEDAYDKGRL
MGTFAEDCAEAYQFTREAQDAFAISSLTRAQNAIKDGLFAAEITPVKVKSGRAEVEVTIDEQPGKAKLDKIPTLRPAFRE
GGTVTAANSSSISDGAAALLLMRASEAEKRGLTPHAVITGHATYADKPNLFSTAPIGAIRKLSEKTGWDLKDVDLFEINE
AFAVVAMAAMRDLDLPHDKVNIHGGACALGHPIGASGARILVTLLAALETHGLKRGIAGICLGGGEATAMGIERII

Sequences:

>Translated_396_residues
MSKSDPIVIVGASRTPMGGFQGDFTNAQATDLGASAIGGALAGAGLAPEAVEEVIMGCVLPAGQGQAPARQASLKAGLPL
GTGATTVNKMCGSGMKAAMLAHDLILAGSADVIVAGGMESMTNAPYLLPKARGGYRMGHGQVLDHMFLDGLEDAYDKGRL
MGTFAEDCAEAYQFTREAQDAFAISSLTRAQNAIKDGLFAAEITPVKVKSGRAEVEVTIDEQPGKAKLDKIPTLRPAFRE
GGTVTAANSSSISDGAAALLLMRASEAEKRGLTPHAVITGHATYADKPNLFSTAPIGAIRKLSEKTGWDLKDVDLFEINE
AFAVVAMAAMRDLDLPHDKVNIHGGACALGHPIGASGARILVTLLAALETHGLKRGIAGICLGGGEATAMGIERII
>Mature_395_residues
SKSDPIVIVGASRTPMGGFQGDFTNAQATDLGASAIGGALAGAGLAPEAVEEVIMGCVLPAGQGQAPARQASLKAGLPLG
TGATTVNKMCGSGMKAAMLAHDLILAGSADVIVAGGMESMTNAPYLLPKARGGYRMGHGQVLDHMFLDGLEDAYDKGRLM
GTFAEDCAEAYQFTREAQDAFAISSLTRAQNAIKDGLFAAEITPVKVKSGRAEVEVTIDEQPGKAKLDKIPTLRPAFREG
GTVTAANSSSISDGAAALLLMRASEAEKRGLTPHAVITGHATYADKPNLFSTAPIGAIRKLSEKTGWDLKDVDLFEINEA
FAVVAMAAMRDLDLPHDKVNIHGGACALGHPIGASGARILVTLLAALETHGLKRGIAGICLGGGEATAMGIERII

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=47.4619289340102, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=45.9390862944162, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=35.9897172236504, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=35.5, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=31.1163895486936, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=28.1725888324873, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=43.4447300771208, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=49.2307692307692, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1787663, Length=393, Percent_Identity=37.1501272264631, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=37.5621890547264, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788683, Length=404, Percent_Identity=29.7029702970297, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17535921, Length=391, Percent_Identity=44.2455242966752, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=40.5684754521964, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=37.4358974358974, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17535917, Length=391, Percent_Identity=32.7365728900256, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=31.4420803782506, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=47.2361809045226, Blast_Score=335, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=46.1538461538462, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=45.8646616541353, Blast_Score=317, Evalue=7e-87,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=36.2025316455696, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.7757009345794, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40746; Mature: 40615

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSDPIVIVGASRTPMGGFQGDFTNAQATDLGASAIGGALAGAGLAPEAVEEVIMGCVL
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCC
PAGQGQAPARQASLKAGLPLGTGATTVNKMCGSGMKAAMLAHDLILAGSADVIVAGGMES
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHEECCCCEEEECCHHH
MTNAPYLLPKARGGYRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEDCAEAYQFTREAQD
HCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
AFAISSLTRAQNAIKDGLFAAEITPVKVKSGRAEVEVTIDEQPGKAKLDKIPTLRPAFRE
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEEEEECCCCCCHHCCCCCCCHHHHC
GGTVTAANSSSISDGAAALLLMRASEAEKRGLTPHAVITGHATYADKPNLFSTAPIGAIR
CCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCHHHHH
KLSEKTGWDLKDVDLFEINEAFAVVAMAAMRDLDLPHDKVNIHGGACALGHPIGASGARI
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCHHHH
LVTLLAALETHGLKRGIAGICLGGGEATAMGIERII
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
SKSDPIVIVGASRTPMGGFQGDFTNAQATDLGASAIGGALAGAGLAPEAVEEVIMGCVL
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCC
PAGQGQAPARQASLKAGLPLGTGATTVNKMCGSGMKAAMLAHDLILAGSADVIVAGGMES
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHEECCCCEEEECCHHH
MTNAPYLLPKARGGYRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEDCAEAYQFTREAQD
HCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
AFAISSLTRAQNAIKDGLFAAEITPVKVKSGRAEVEVTIDEQPGKAKLDKIPTLRPAFRE
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEEEEECCCCCCHHCCCCCCCHHHHC
GGTVTAANSSSISDGAAALLLMRASEAEKRGLTPHAVITGHATYADKPNLFSTAPIGAIR
CCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCHHHHH
KLSEKTGWDLKDVDLFEINEAFAVVAMAAMRDLDLPHDKVNIHGGACALGHPIGASGARI
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCHHHH
LVTLLAALETHGLKRGIAGICLGGGEATAMGIERII
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]