Click here to switch to the map view.

The map label for this gene is bepF [H]

Identifier: 163844514

GI number: 163844514

Start: 316765

End: 318024

Strand: Reverse

Name: bepF [H]

Synonym: BSUIS_B0341

Alternate gene names: 163844514

Gene position: 318024-316765 (Counterclockwise)

Preceding gene: 163844515

Following gene: 163844513

Centisome position: 22.7

GC content: 59.52

Gene sequence:

>1260_bases
ATGGTTGCCTTTTGGACGTGCCGAAACGCTTGGTTCCAACACCTTCCTTTTGCGAAGCGCGGCGACGAAAATGCGCCATC
TGGACCACGCCGGCTGCGCCCCTGGTTCCTCGTTCTGGCTCTGGGGCTTGCAGCGTGCAGCGAAGACAAGTCAGCTCCGC
AGCAAGCCGCTCCCCTGCCCCCGATACCTGTCGGCGTCATCAAGATAACAGAGCGCCCCACCCATCCCCAGCTCTCCTTC
GTCGGACGTGTCGAGGCAACCGATAGCGTCGATCTGATAGCCCGCGTCGATGGCTTTCTCGACAAGCGCACCTTCACCGA
AGGTCAGGCCGTAAAGACCGGGGATTTGCTGTTTGCCCTGCAAAAGGATGCGCTTCAGGCGGCGCTGGATGGGGCGCAGG
CCAATCTTGCAAAAGCGCAGGCCGATGCCGACAACCTGAAACTCCAGACCGAGCGCGCCCGCTCGCTCTACAAGCAGAAG
ACCGTTTCGCAAGCCATGCTTGATGATCGTGTGGCAGCGGAAAAGCAGGCTTTGGCCGTCGTGCAGCAGGCGCAGGCTTC
GCTGGAACAGGCGCAAATCAATCTCGGCTATACCGATATACGCGCGCCTTTTTCCGGACGCATCGGCATGGCCAATTTCA
GCGTCGGCGCCCTTGTCGGGCCGTCCTCAGGCCCTCTGGCAACGATCGTCAGCCAGGACCCGATTTACGTCACCTTCCCC
GTCAGCGACAAGACCATTCTCGATCTAACCGAAGGCGGGAGAACGGCCACAGACCGCAGCAATGTAGCCGTCAGCCTGAC
GCTCTCCAACGGCATGACATATCCGCAAACGGGGGCCATCGACTTTACCGGGATCAAGATTAATCCCAATACCGATACGC
TGATGGTCCGGGCACAGTTTCCCAATCCGAACAATGTCCTGATCGACGGGCAGTATGTTCAGGTAACGGCGGCGAGCAAA
TACCCCGTTGAAGCCCTGCTCGTCCCGCAAAAGGCAATCATGACCGACCAGAGCGGCAATTACGTTCTGGCCGTCGGTGA
AGATAACAAGGTCATCCAGCGCCAGATCACGCAAGGTTCGACCTTTGGCAGCAATGTTGTGGTCAAGTCCGGCCTCGCGG
TGGGCGATCAGGTCGTGGTCGATGGCCTCCAGCGCATCCGGCCCGGCCAGAAGGTCGATCCGCAGATCGTCGATGCAACC
ACGCCCGCCCAAAGCCATGTCGGTGGGGAATTGATCCATGCTGTCATCCGTCTTCATTAA

Upstream 100 bases:

>100_bases
AACCGGCAGCCCTGCAAAAATCGCACGGCGCTTAGCTGGGCCTGAGCGAAATATAACCCGGCCTGTTGACGAACGGGTCG
ATATGCATGGAGTGCTGACG

Downstream 100 bases:

>100_bases
CAGGCCGCGCCTTGCCATTGTGATCGCTATCGTCATCACGCTGGCAGGGCTGATCGCGGTCACACGCATTCCCGTCGCGC
AATTTCCCGATATTGTTCCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFALQKDALQAALDGAQANLAKAQADADNLKLQTERARSLYKQK
TVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFP
VSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASK
YPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDAT
TPAQSHVGGELIHAVIRLH

Sequences:

>Translated_419_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFALQKDALQAALDGAQANLAKAQADADNLKLQTERARSLYKQK
TVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFP
VSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASK
YPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDAT
TPAQSHVGGELIHAVIRLH
>Mature_419_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFALQKDALQAALDGAQANLAKAQADADNLKLQTERARSLYKQK
TVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFP
VSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASK
YPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDAT
TPAQSHVGGELIHAVIRLH

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=390, Percent_Identity=34.6153846153846, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=35.149863760218, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1789665, Length=366, Percent_Identity=33.0601092896175, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082036, Length=417, Percent_Identity=29.4964028776978, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081951, Length=172, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081796, Length=348, Percent_Identity=26.7241379310345, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44912; Mature: 44912

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLP
CEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCC
PIPVGVIKITERPTHPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFAL
CCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEE
QKDALQAALDGAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAV
HHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFP
HHHHHHHHHHHEEECCEEEECCCCCCCEEEEECCEEEEECCCCCCEEEEECCCCEEEEEE
VSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQF
CCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEEC
PNPNNVLIDGQYVQVTAASKYPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGS
CCCCEEEECCEEEEEEECCCCCEEEEEECCHHHEECCCCCEEEEECCCCHHHHHHHHCCC
TFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQSHVGGELIHAVIRLH
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLP
CEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCC
PIPVGVIKITERPTHPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFAL
CCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEE
QKDALQAALDGAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAV
HHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFP
HHHHHHHHHHHEEECCEEEECCCCCCCEEEEECCEEEEECCCCCCEEEEECCCCEEEEEE
VSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQF
CCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEEC
PNPNNVLIDGQYVQVTAASKYPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGS
CCCCEEEECCEEEEEEECCCCCEEEEEECCHHHEECCCCCEEEEECCCCHHHHHHHHCCC
TFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQSHVGGELIHAVIRLH
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]