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The map label for this gene is fumC [H]

Identifier: 163844380

GI number: 163844380

Start: 177301

End: 178692

Strand: Reverse

Name: fumC [H]

Synonym: BSUIS_B0195

Alternate gene names: 163844380

Gene position: 178692-177301 (Counterclockwise)

Preceding gene: 163844381

Following gene: 163844379

Centisome position: 12.76

GC content: 59.77

Gene sequence:

>1392_bases
ATGGCCGCCACGCGTACCGAAACAGACACTTTCGGACCGATCGATGTTCCCGCAGACCGTTATTGGGGCGCACAGACACA
GCGTTCCCTGCAAAATTTCAGGATCGGCGGCGAGCGCATGCCCCTGCCCCTGGTTCACGCGCTGGGCGTGGTAAAGCGCG
CGGCGGCGGAAACCAATATCGCGCTCGGCAAGCTTGATCCCGTCCTGGGTCAGGTGATTGCGGTTGCCGCTTCCGAAGTC
ATTGAGGGCAAGCTCGACGACCATTTCCCGCTCGTAGTCTGGCAGACAGGCTCCGGCACGCAATCGAACATGAATGCCAA
TGAAGTGATCTCGAACCGGGCTATCGAGTTGCTCGGCGGGGAAATGGGCTCCAAAAAGCCGATCCACCCGAACGACCATG
TCAATATGAGCCAGTCTTCCAATGACAGTTTCCCGACGGCGATCCACATCGCCATGGCGGTCGAAACCGTCAACCGGCTC
TATCCCGCGCTCGAACACCTCACCAAAGCCCTGAAGGTCAAGGAAGAAGCATTCAAGGACATCATCAAGATTGGCCGCAC
CCACACGCAGGACGCAACACCTGTGACGCTGGGGCAGGAGTTTTCGGGCTACCGCGCGGCACTTGAATATGCCCGCCATC
GGCTCGAACAATCGCTTGCCGACGTCTTCCTGCTGGCGCAGGGCGGCACGGCTGTCGGCACCGGTCTCAACGCACCTGTC
GGTTTCGACAAGGGCTTTGCCGAAGCGGTGAGCGAGATTACCGGGCTTTCCTTCAAGACCGCGCCAAACAAGTTCGAGGC
GCTGGCAAGCCACGGCGCCGTTCTCAACTTCCACGGCAGCCTGAATGCGCTTGCAGCCGATCTCTTCAAGATCGCCAACG
ATATCCGGTTTCTCGGTTCCGGCCCGCGTTCCGGCCTTGGCGAGCTTTCTCTGCCGGAAAACGAACCCGGCTCCTCCATC
ATGCCGGGCAAGGTCAACCCCACACAGGCCGAAGCCATGACCATGGTGGCAACCCAGGTTTTCGGCAACCAGACCGCCGT
AACCGTCGCCGCCAGCCAGGGGCATTTCGAGCTGAACGTCTTCAAACCGGTCATTGCCTATAATGTGCTGCAATCGATCC
GGCTTTTGAGCGACACCATGGTTTCCTTTGCCGATCATTGCGTCGAGGGGATTGAACCCAATACGGCCCGCATCAAGGAA
TTGCTGGAACGCTCGCTCATGCTCGTCACGGCGCTTGCGCCTGCCATCGGCTATGACAATGCGGCCAGGATCGCCAAAAC
CGCCCATAAGAACGGCACCACGCTTCGCGAGGAAGCATTGGCAAGCGGCCTCGTATCGGAAGAAGATTATGACCGGCTGG
TGCGCGCCGAGCGGATGATCGCCCCACAATGA

Upstream 100 bases:

>100_bases
TCATGTGTTCATCAATTAAACGTTTTGCCCGCCAGCAATCTGGCAGTGCTATAGAATGCGAAAAGGGCTCGTCCGAGCCT
CAGCCAGCCGGAGACATGAA

Downstream 100 bases:

>100_bases
ACTGCACGCAAATAGTGAACAAACTGTCGAGAATTAAGCATCTTTTCTTGACAATTACGAATCGCTAATTGATGGATCAA
TTCAACAGGAGAAAGCCCAC

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEV
IEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIAMAVETVNRL
YPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPV
GFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSI
MPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKE
LLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ

Sequences:

>Translated_463_residues
MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEV
IEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIAMAVETVNRL
YPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPV
GFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSI
MPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKE
LLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ
>Mature_462_residues
AATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEVI
EGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIAMAVETVNRLY
PALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPVG
FDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIM
PGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKEL
LERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=63.7744034707158, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI266458395, Length=66, Percent_Identity=54.5454545454545, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1787896, Length=460, Percent_Identity=58.2608695652174, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=37.7440347071584, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=65.8064516129032, Blast_Score=629, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=64.9390243902439, Blast_Score=432, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=63.9913232104121, Blast_Score=604, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=64.2857142857143, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=64.2857142857143, Blast_Score=606, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=61.3390928725702, Blast_Score=580, Evalue=1e-165,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=60.9071274298056, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=53.2327586206897, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 49655; Mature: 49524

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNI
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
ALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
EMGSKKPIHPNDHVNMSQSSNDSFPTAIHIAMAVETVNRLYPALEHLTKALKVKEEAFKD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPV
HHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCCC
GFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGS
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
GPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEH
FKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNI
CCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
ALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
EMGSKKPIHPNDHVNMSQSSNDSFPTAIHIAMAVETVNRLYPALEHLTKALKVKEEAFKD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPV
HHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCCC
GFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGS
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
GPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEH
FKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]