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The map label for this gene is gatA [H]

Identifier: 163844297

GI number: 163844297

Start: 95864

End: 97069

Strand: Reverse

Name: gatA [H]

Synonym: BSUIS_B0104

Alternate gene names: 163844297

Gene position: 97069-95864 (Counterclockwise)

Preceding gene: 163844299

Following gene: 163844296

Centisome position: 6.93

GC content: 60.12

Gene sequence:

>1206_bases
ATGGCCTATCTTGATCCTTTCAATGCCCTCATTGCCAAACCTGAAAAACCCCTTGCCCATGCCGCAAGCGGCCCGCTTGC
AGGTGAAAGGCTGGCGGTCAAGGACATTTATGACATTGCCGGAATGGTGACCGGATGCGGAAATCCACAAATTCTGGCCG
AATCACCCGTCGCCCGGAAATCCGCCCCAGTGGTGGAAAAACTGCTTGCGGCCGGCGCCGAATTCATCGGCAAGGCGCAG
ACGGATGAAGTGGCTTTTTCCATGATGGGCCAGAATTCGCATTACCCCTATCCGATCAATCCGGCGGCCCCTGATCGCGT
TACTGGCGGCTCCTCATCCGGTTCGGCTGCGGCAGTCGCGGGAAAGCTTGCCAATATCGCGCTCGGCTCGGACACGGGCG
GCTCCATCCGCACGCCTGCAAGCTTTTGCGGGCTGATTGGCCTGCGCTCCGCCCATGGCCGCATTCCGCTCGAAGGCATC
ATGTCGCTTGCGCCTTCGCTGGATACTATCGGCTGGTTCGCACGCGATATTGCGCTTTATGAAAAGGTGGGCGCGGTCCT
GCTTGGCGACGACGCACAGGAATTCAAGCTGACCCAGCTTCTCTATATGCCGGTTTTGGAACAGCTCTTGCTGGGGCAAG
CTGAAACCGATGCCTACCGCGCCATGTTTGCCAAGGTGCGCCCGCATTTTGCAACTCTCAAGGCGGCAAGCCAGCCGACG
CTTTCCATTGATGAACTGTATCTCGCCTTCCGCCAGATACAGGGCGCGGAACCATGGGAAAACCATGGCGCATGGATTTC
CTCCGGCAATCGCCAGCTTGGCCCCGGTATTGCCGACCGCTTCGCTTACGGTGCCGAAATGACCGCCGATATGGTCAAAA
ACCAGCGCGCAAGGCGCGCCCGCTTCGCGGAAGAATTTGAGGCCATTATCGGCGAAAGCGCCGTTCTGGCGCTGCCAACC
GTGCCGGGCGCGGCCCCGCTCACCGGCGAACCTTTCGAGGCATTGCAGGCCTATCGCGAACAGGCCCTGCGCCTGCTCTG
CCTTTCGGGGCTTTCGGGCCTGCCACAGATTACATTACCCCTGGGATCATTGAGGCCGGGCGGCACGATGCATGACGCAC
CCTTTGGCATTTCCTTCATCGGACCACGCGGCAGCGACCGGACGCTGATCGCGCTGGCTCAAACCATCATCAGCGAGCGA
AGTTAG

Upstream 100 bases:

>100_bases
ACCCTATGCTTGCCATTGTTCAATATAAGTGAAACATTTCGATGTCGGACTTCCGTTCTGACGCCCCAAAAAACCTTTTA
TTTATCGCATGTTGTGAACA

Downstream 100 bases:

>100_bases
ACCATGGACACGCTGACACGCATGCGCGCCTTCATCGACGTGGTCGAGGCGGAAGGCTTTTCCGCCGCAGCGCGCAAGAT
CGGGCGGTCCAAGGCACTTC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAYLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARKSAPVVEKLLAAGAEFIGKAQ
TDEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVAGKLANIALGSDTGGSIRTPASFCGLIGLRSAHGRIPLEGI
MSLAPSLDTIGWFARDIALYEKVGAVLLGDDAQEFKLTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAASQPT
LSIDELYLAFRQIQGAEPWENHGAWISSGNRQLGPGIADRFAYGAEMTADMVKNQRARRARFAEEFEAIIGESAVLALPT
VPGAAPLTGEPFEALQAYREQALRLLCLSGLSGLPQITLPLGSLRPGGTMHDAPFGISFIGPRGSDRTLIALAQTIISER
S

Sequences:

>Translated_401_residues
MAYLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARKSAPVVEKLLAAGAEFIGKAQ
TDEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVAGKLANIALGSDTGGSIRTPASFCGLIGLRSAHGRIPLEGI
MSLAPSLDTIGWFARDIALYEKVGAVLLGDDAQEFKLTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAASQPT
LSIDELYLAFRQIQGAEPWENHGAWISSGNRQLGPGIADRFAYGAEMTADMVKNQRARRARFAEEFEAIIGESAVLALPT
VPGAAPLTGEPFEALQAYREQALRLLCLSGLSGLPQITLPLGSLRPGGTMHDAPFGISFIGPRGSDRTLIALAQTIISER
S
>Mature_400_residues
AYLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARKSAPVVEKLLAAGAEFIGKAQT
DEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVAGKLANIALGSDTGGSIRTPASFCGLIGLRSAHGRIPLEGIM
SLAPSLDTIGWFARDIALYEKVGAVLLGDDAQEFKLTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAASQPTL
SIDELYLAFRQIQGAEPWENHGAWISSGNRQLGPGIADRFAYGAEMTADMVKNQRARRARFAEEFEAIIGESAVLALPTV
PGAAPLTGEPFEALQAYREQALRLLCLSGLSGLPQITLPLGSLRPGGTMHDAPFGISFIGPRGSDRTLIALAQTIISERS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI222831590, Length=177, Percent_Identity=35.5932203389831, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=413, Percent_Identity=24.455205811138, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=415, Percent_Identity=26.0240963855422, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=137, Percent_Identity=34.3065693430657, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=442, Percent_Identity=25.7918552036199, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=169, Percent_Identity=37.8698224852071, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648113, Length=159, Percent_Identity=37.1069182389937, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 42353; Mature: 42222

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARK
CCCCCCHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
SAPVVEKLLAAGAEFIGKAQTDEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
GKLANIALGSDTGGSIRTPASFCGLIGLRSAHGRIPLEGIMSLAPSLDTIGWFARDIALY
HHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
EKVGAVLLGDDAQEFKLTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAASQPT
HHHCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCC
LSIDELYLAFRQIQGAEPWENHGAWISSGNRQLGPGIADRFAYGAEMTADMVKNQRARRA
CHHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
RFAEEFEAIIGESAVLALPTVPGAAPLTGEPFEALQAYREQALRLLCLSGLSGLPQITLP
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGSLRPGGTMHDAPFGISFIGPRGSDRTLIALAQTIISERS
CCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AYLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARK
CCCCCHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
SAPVVEKLLAAGAEFIGKAQTDEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
GKLANIALGSDTGGSIRTPASFCGLIGLRSAHGRIPLEGIMSLAPSLDTIGWFARDIALY
HHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
EKVGAVLLGDDAQEFKLTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAASQPT
HHHCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCC
LSIDELYLAFRQIQGAEPWENHGAWISSGNRQLGPGIADRFAYGAEMTADMVKNQRARRA
CHHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
RFAEEFEAIIGESAVLALPTVPGAAPLTGEPFEALQAYREQALRLLCLSGLSGLPQITLP
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGSLRPGGTMHDAPFGISFIGPRGSDRTLIALAQTIISERS
CCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA