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The map label for this gene is virB4 [H]

Identifier: 163844264

GI number: 163844264

Start: 62962

End: 65457

Strand: Reverse

Name: virB4 [H]

Synonym: BSUIS_B0070

Alternate gene names: 163844264

Gene position: 65457-62962 (Counterclockwise)

Preceding gene: 163844265

Following gene: 163844263

Centisome position: 4.67

GC content: 55.61

Gene sequence:

>2496_bases
ATGGGCGCTCAATCCAAATACGCGCAACAGCTCAATAATGAACGGTCACTTGCACCGTTCATTCCCTTCAGGAGCCAGGT
TGGCCCGACCACCGTGATAACGCGTGACGGTGATTTCGTGCGCACATGGCGCATCGCGGGCCTGGCTTTCGAGACGCAGG
ACAAGGAGGAACTGTTGATCCGCAAGGACCAGCTTAACACCTTGTTCCGTGCCATCGCCTCAAACAATGTGGCGCTGTGG
TCCCACAACGTACGGCGCAGAACCTGGGATCACTTGAAATCCTTCTTTTCCAATCCGTTCTGCGACGCTCTCGACAAGAA
GTATTACGGATCGTTTTCCGGCTACCGAATGATGTCAAACGAACTGTATCTGACGGTTATTTACCGGCCAGTGCCGGCGA
AGATCAGCAGGTTGTTCAACGTGGCCGTTCATCGTTCGCATGCGGAAATCTTACAGGAGCAGCAGCTTGCCATCCGCAAG
CTGGACGAAATCGGCAATCAAATTGAAACCAGCTTGCGTCGCTACGGTGGCGATGACGGGCGTGGCATCGAAGTGCTGAG
CACCTATGAGGACAAACACGGCGCACTGTGCTCACAGCAGCTTGAATTTTATAACTTCCTTCTCTCAGGCGAATGGCAGA
AGGTGCGCGTGCCATCCTGCCCACTGGATGAATATCTGGGCACCGGGTGGGTGTATGCTGGCACTGAAACCATCGAGATA
CGCACCGCGAACGCGACGCGCTATGCGCGTGGCATCGATTTCAAGGACTATGCGAGCCACACCGAGCCGGGCATTTTGAA
CGGCCTGATGTATAGCGATTACGAGTACGTCATCACACAATCCTTCAGCTTCATGACAAAGCGGGATGGCAAGGAGTTCC
TGACACGGCAAAAGCAGCGGCTCCAAAATACCGAAGACGGCAGCGCATCCCAGATCATGGAAATGGACATTGCCATCGAC
CAGCTCGGCCGGGGCGATTTCGTCATGGGTGAATATCATTATTCGCTGCTGGTATTTGCCGAAGACATGGAGACGGTGCG
CCACAACACATCACATGCGATGAACATCCTGCAAGACAATGGTTTTCTGGCGACGGTGATCGCCACGGCCACGGATGCGG
CGTTCTATGCACAACTGCCATGTAACTGGCGGTATCGGCCGCGCGTGGCGGGCCTGACCTCGCTCAACTTCGCCGGTCTG
TCATGTTTCCATAATTTCCGCGCAGGCAAGCGGGATGGCAACCCCTGGGGGCAGGCATTGACGCTGCTCAAAACCCCTTC
CGGACAGCCCGCCTACCTGAACTTCCACTATTCAAAAGGCGACGAAGACAACTTCGACAAGAAGCTTCTGGGCAATACCC
GCATCATCGGCCAGTCCGGCGCTGGTAAAACTGTCCTGATGAACTTCTGCCTCGCGCAAGCGCAGAAGTATTTGCACAAT
GCTCCGATGGGTATGTGCAACGTCTTTTTCGACAAGGATCAAGGCGCGAAAGGGACTATTCTTGCCATTGGCGGCAAATA
CCTTGCCATCAGGAACGGCGAACCCACCGGGTTCAACCCATTCCAGATGGAGCCAACAGCGGGGAACATCCTGTTCCTTG
AAAAACTCGTACAGGTACTGGTATCGCGTGATGGCCAGCACGTGACCACAACGGACGAAAGCCGGATCAGTCATGCCATA
CGTACGGTCATGCGCATGCGCCCGGAGCTGCGGCGTCTGTCGACGGTGCTGCAAAACGTTACCGAAGGCAGCGACCGCCA
AGATCGTGAAAACAGCGTGGCGAAGCGGCTCGCGAAGTGGTGTTTTGATGACGGCACCGGCAAGCGGGGCACATTCTGGT
GGGTGCTGGATTGCCCGCAAGACCAGATCGACTTCAACACGCATAGCAATTACGGTTTCGATGGCACCGACTTCCTCGAC
AACGCCGACGTGCGTACGCCGATTTCCATGTACCTGCTGCATCGTATGGAATTGGCTATCGACGGGCGTCGTTTCATCTA
CTGGATGGACGAGGCGTGGAAGTGGGTAGACGACGAAGCATTCAGCGAGTTCGCCAATAACAAGCAGCTTACCATCCGCA
AGCAAAACGGCCTGGGCGTGTTTGCCACGCAAATGCCCTCCAGCCTGCTCAACTCCAAAGTCGCGTCGGCCCTGGTGCAA
CAGGTGGCGACGGAAATCTATCTGCCGAATCCGAAAGCCGATTATCACGAGTACACGGACGGCTTCAAGGTGACGAACGA
GGAGTTCGACATCATCCGCAGTATGAGCGAGGAAAGCCGCATGTTCCTGGTCAAGCAGGGGCATCATTCGATGATTTGCC
GCCTGGAGCTTAATGGCTTTGATGACGAACTGGCGATCCTCTCAGGCAGCTCGGACAACAACGAGTTGCTGGACCAGGTC
ATTGCCGAAGTCGGGGACGACCCTTCGGTATGGCTTCCGGTGTTCCAGGAGCACCGGAAAGCACGCATTGCGTCGTCCAA
ATCAACAGGAAGGTGA

Upstream 100 bases:

>100_bases
AGGCGCAATTCCGCCTGATCCACTTCAACCGCACCGGGCGCTTCTGGCGGGCATCCGCCTATAGCCCGATTGCCTTCACA
AAGCGCAAAAGAGAATCATG

Downstream 100 bases:

>100_bases
CACTATGAAGAAGATAATTCTCAGCTTCGCATTCGCCCTGACTGTAACCAGCACGGCGCACGCACAGCTCCCGGTGACAG
ATGCCGGCTCCATCGCGCAG

Product: type IV secretion/conjugal transfer ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALW
SHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRK
LDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEI
RTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAID
QLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGL
SCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHN
APMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAI
RTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLD
NADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQ
QVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQV
IAEVGDDPSVWLPVFQEHRKARIASSKSTGR

Sequences:

>Translated_831_residues
MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALW
SHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRK
LDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEI
RTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAID
QLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGL
SCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHN
APMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAI
RTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLD
NADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQ
QVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQV
IAEVGDDPSVWLPVFQEHRKARIASSKSTGR
>Mature_830_residues
GAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALWS
HNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKL
DEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEIR
TANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAIDQ
LGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLS
CFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHNA
PMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAIR
TVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDN
ADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQQ
VATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQVI
AEVGDDPSVWLPVFQEHRKARIASSKSTGR

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 94528; Mature: 94397

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLI
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCHHHHHH
RKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSN
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECC
ELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKLDEIGNQIETSLRRYGGDDG
CEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEI
CCEEEEEECCCHHCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCEEEECCEEEEE
RTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQR
EECCCHHHHCCCCCHHHCCCCCCCHHCCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHH
LQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDN
HCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHHHHHHCCCHHHHHHHHCC
GFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQAL
CEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHEE
TLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHN
EEEECCCCCCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
APMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVL
CCCCEEEEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHH
VSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKW
HCCCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAI
HCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEE
DGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQ
CCCEEEEEECCHHCCCCHHHHHHHCCCCEEEEEECCCCEEEHHHCCHHHHHHHHHHHHHH
QVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGF
HHHHHEECCCCCCCHHHHCCCCEECCHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCC
DDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQEHRKARIASSKSTGR
CCCEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLI
CCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCHHHHHH
RKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSN
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECC
ELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKLDEIGNQIETSLRRYGGDDG
CEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEI
CCEEEEEECCCHHCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCEEEECCEEEEE
RTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQR
EECCCHHHHCCCCCHHHCCCCCCCHHCCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHH
LQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDN
HCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHHHHHHCCCHHHHHHHHCC
GFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQAL
CEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHEE
TLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHN
EEEECCCCCCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
APMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVL
CCCCEEEEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHH
VSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKW
HCCCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAI
HCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEE
DGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQ
CCCEEEEEECCHHCCCCHHHHHHHCCCCEEEEEECCCCEEEHHHCCHHHHHHHHHHHHHH
QVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGF
HHHHHEECCCCCCCHHHHCCCCEECCHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCC
DDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQEHRKARIASSKSTGR
CCCEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]